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The map label for this gene is alkJ [H]

Identifier: 93006670

GI number: 93006670

Start: 2278510

End: 2280153

Strand: Direct

Name: alkJ [H]

Synonym: Pcryo_1846

Alternate gene names: 93006670

Gene position: 2278510-2280153 (Clockwise)

Preceding gene: 93006669

Following gene: 93006672

Centisome position: 74.46

GC content: 46.84

Gene sequence:

>1644_bases
ATGGAACTTGATGGAAACTTTGATTACGTCATCGTTGGTGGCGGTTCAGCTGGCTGTGTGCTTGCCAGCCGATTAACAGA
AAACCCAGACATCAGTGTCTGCTTGTTAGAATATGGTGGTGAGGGCAAAGACTTGGCAGTTCGCGTACCTGCTGGACTGA
TTTTGATGGTGCCGGGCAAGCCACTAAAATTAAACAATTGGTGCTTTCACACTACTCCGCAAATCCATCTTAATAACCGA
CGCGGCTTTCAACCGCGCGGTCAATGTTTGGGTGGCTCCTCCGCTATCAATGCCATGATTTATACGCGTGGCAGTGCGCT
TGATTATGAACGCTGGGTTGAGCAAGGTTGCACAGGCTGGGGTTTTGATGATGTACTGCCTTACTTTATCAAATCAGAAA
ACAATATCCACGGCGCGGACGAGCTGCATGGTGATAGTGGACCACTGCATGTCAGTGATTTACTAAGTCCACGCGATATC
TCAAAAGCTTTTGTAGAAGCTGCTATTGCCAATGGTTTAGATCAAAATGATGACTTTAACGGCAAAAAACAAGACGGTGC
AGGATTATATCAAGTCACCCATTTTCATGGGGAAAAACAAGGTCAACGCTGTTCTGCCGCTGCTGCTTATCTACATCCAG
TACAAAGCCGACCCAATCTAACCGTCATCACCCACGCACAAGCTGACCGCGTCATCTTTGAAGACAAACAAGCGACTGGC
GTGATCTATGAAAAGGATGGTGTCGAGCATACGGTTATGGCTCGCCATGAAGTGATATTATCAGGCGGCACTTTTGGCTC
ACCCAAAGTTTTGATGCTATCAGGCATTGGTCCTGCTGAACATTTGCAGTCTCATGGCATCGAGGTATTGGTAGATGCGC
CCGACGTTGGCGGTAACTTACAAGATCATTTGGACGTCGTTTTTGATTATGAAGTTAATACTACTGATGTCATCGGTATT
GGTATAGCAACTATTTCAACCTTGACCAAAAGTATTCGGCAATGGCGAAAAGATGGCACAGGTCTGTTATCTACCAATTA
TGCAGAAGCTGGCGCGTTCTTTAGCGTCGGTGACGACCCACAAGAATGGCCAAATACCCAGCTGCATTTTGTGATATCTC
GCGTGATTGAACACGGACGTGATCTTAGAAGGGGATTTGCCGTTTCTTGTCATACCTGCTATTTACGCCCTGAAAGCCGT
GGTACGGTCAGACTCGATTCTGCCAATCCCTCTGACGCCGTGTTAATTGATCCAAACTATCTTTCGCATCCGAAAGATGT
AGAGTATATGGTCGCTGGTGCAGAGCGTACGCGCGCCATTATGCAAGAGTCGCCAATGGCTGAATACATCACTGAGGACT
ACCCTGCCCCCTATATCGAAAAAGACGGTATGCTTGGCTATATCCGTAATAAATCAGACACTATCTACCACCCTGTAGGC
ACTTGCCGTATGGGCTCAGACAGCGATTCAGTCGTTGATTTAGAATTAAAAGTACGCGGCGTAAATGGACTAAGGGTCAT
CGATGCTTCAGTGATGCCAACCTTAATCAGTGCCAATACCAACGCACCGACGATTATGATTGCTGAAAAAATAGCAGACC
TTATCAAGGCCAATCACGTGCATGCTGAGTCAGTCCCTGCTTAA

Upstream 100 bases:

>100_bases
ATTTTTACTTCATTATTGCAGCAAAATAATAGATACTCTAAAGCGTAAGCTATTTCAATAAATACGTTTTAATGAGCATG
GATGCTACAGGGAATATAAA

Downstream 100 bases:

>100_bases
CATAGTTTAAGATGATTAATCGAGCCCAAAATCAAGTTTCATTTGGGCTCAATTTGTATTGACTACTCCGCGACATTTTG
GAAATTGGCTACAACCAAAA

Product: glucose-methanol-choline oxidoreductase

Products: Betaine Aldehyde; Reduced Acceptor. [C]

Alternate protein names: NA

Number of amino acids: Translated: 547; Mature: 547

Protein sequence:

>547_residues
MELDGNFDYVIVGGGSAGCVLASRLTENPDISVCLLEYGGEGKDLAVRVPAGLILMVPGKPLKLNNWCFHTTPQIHLNNR
RGFQPRGQCLGGSSAINAMIYTRGSALDYERWVEQGCTGWGFDDVLPYFIKSENNIHGADELHGDSGPLHVSDLLSPRDI
SKAFVEAAIANGLDQNDDFNGKKQDGAGLYQVTHFHGEKQGQRCSAAAAYLHPVQSRPNLTVITHAQADRVIFEDKQATG
VIYEKDGVEHTVMARHEVILSGGTFGSPKVLMLSGIGPAEHLQSHGIEVLVDAPDVGGNLQDHLDVVFDYEVNTTDVIGI
GIATISTLTKSIRQWRKDGTGLLSTNYAEAGAFFSVGDDPQEWPNTQLHFVISRVIEHGRDLRRGFAVSCHTCYLRPESR
GTVRLDSANPSDAVLIDPNYLSHPKDVEYMVAGAERTRAIMQESPMAEYITEDYPAPYIEKDGMLGYIRNKSDTIYHPVG
TCRMGSDSDSVVDLELKVRGVNGLRVIDASVMPTLISANTNAPTIMIAEKIADLIKANHVHAESVPA

Sequences:

>Translated_547_residues
MELDGNFDYVIVGGGSAGCVLASRLTENPDISVCLLEYGGEGKDLAVRVPAGLILMVPGKPLKLNNWCFHTTPQIHLNNR
RGFQPRGQCLGGSSAINAMIYTRGSALDYERWVEQGCTGWGFDDVLPYFIKSENNIHGADELHGDSGPLHVSDLLSPRDI
SKAFVEAAIANGLDQNDDFNGKKQDGAGLYQVTHFHGEKQGQRCSAAAAYLHPVQSRPNLTVITHAQADRVIFEDKQATG
VIYEKDGVEHTVMARHEVILSGGTFGSPKVLMLSGIGPAEHLQSHGIEVLVDAPDVGGNLQDHLDVVFDYEVNTTDVIGI
GIATISTLTKSIRQWRKDGTGLLSTNYAEAGAFFSVGDDPQEWPNTQLHFVISRVIEHGRDLRRGFAVSCHTCYLRPESR
GTVRLDSANPSDAVLIDPNYLSHPKDVEYMVAGAERTRAIMQESPMAEYITEDYPAPYIEKDGMLGYIRNKSDTIYHPVG
TCRMGSDSDSVVDLELKVRGVNGLRVIDASVMPTLISANTNAPTIMIAEKIADLIKANHVHAESVPA
>Mature_547_residues
MELDGNFDYVIVGGGSAGCVLASRLTENPDISVCLLEYGGEGKDLAVRVPAGLILMVPGKPLKLNNWCFHTTPQIHLNNR
RGFQPRGQCLGGSSAINAMIYTRGSALDYERWVEQGCTGWGFDDVLPYFIKSENNIHGADELHGDSGPLHVSDLLSPRDI
SKAFVEAAIANGLDQNDDFNGKKQDGAGLYQVTHFHGEKQGQRCSAAAAYLHPVQSRPNLTVITHAQADRVIFEDKQATG
VIYEKDGVEHTVMARHEVILSGGTFGSPKVLMLSGIGPAEHLQSHGIEVLVDAPDVGGNLQDHLDVVFDYEVNTTDVIGI
GIATISTLTKSIRQWRKDGTGLLSTNYAEAGAFFSVGDDPQEWPNTQLHFVISRVIEHGRDLRRGFAVSCHTCYLRPESR
GTVRLDSANPSDAVLIDPNYLSHPKDVEYMVAGAERTRAIMQESPMAEYITEDYPAPYIEKDGMLGYIRNKSDTIYHPVG
TCRMGSDSDSVVDLELKVRGVNGLRVIDASVMPTLISANTNAPTIMIAEKIADLIKANHVHAESVPA

Specific function: Converts aliphatic medium-chain-length alcohols into aldehydes. May be linked to the electron transfer chain [H]

COG id: COG2303

COG function: function code E; Choline dehydrogenase and related flavoproteins

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GMC oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI217272839, Length=551, Percent_Identity=41.923774954628, Blast_Score=377, Evalue=1e-104,
Organism=Escherichia coli, GI1786503, Length=550, Percent_Identity=39.6363636363636, Blast_Score=360, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI17532301, Length=552, Percent_Identity=35.8695652173913, Blast_Score=334, Evalue=7e-92,
Organism=Drosophila melanogaster, GI45551458, Length=578, Percent_Identity=37.5432525951557, Blast_Score=327, Evalue=2e-89,
Organism=Drosophila melanogaster, GI45549471, Length=577, Percent_Identity=37.6083188908146, Blast_Score=326, Evalue=2e-89,
Organism=Drosophila melanogaster, GI24642039, Length=582, Percent_Identity=36.9415807560137, Blast_Score=318, Evalue=9e-87,
Organism=Drosophila melanogaster, GI24642055, Length=585, Percent_Identity=35.8974358974359, Blast_Score=317, Evalue=9e-87,
Organism=Drosophila melanogaster, GI24642059, Length=574, Percent_Identity=36.2369337979094, Blast_Score=301, Evalue=8e-82,
Organism=Drosophila melanogaster, GI24642042, Length=580, Percent_Identity=35.8620689655172, Blast_Score=301, Evalue=8e-82,
Organism=Drosophila melanogaster, GI18859995, Length=575, Percent_Identity=35.4782608695652, Blast_Score=300, Evalue=2e-81,
Organism=Drosophila melanogaster, GI24642048, Length=602, Percent_Identity=34.3853820598007, Blast_Score=293, Evalue=2e-79,
Organism=Drosophila melanogaster, GI24642037, Length=576, Percent_Identity=35.9375, Blast_Score=293, Evalue=2e-79,
Organism=Drosophila melanogaster, GI24642035, Length=578, Percent_Identity=35.2941176470588, Blast_Score=283, Evalue=3e-76,
Organism=Drosophila melanogaster, GI24650267, Length=575, Percent_Identity=36.3478260869565, Blast_Score=280, Evalue=1e-75,
Organism=Drosophila melanogaster, GI17137792, Length=567, Percent_Identity=32.0987654320988, Blast_Score=263, Evalue=3e-70,
Organism=Drosophila melanogaster, GI18859993, Length=578, Percent_Identity=31.6608996539792, Blast_Score=256, Evalue=4e-68,
Organism=Drosophila melanogaster, GI24642051, Length=590, Percent_Identity=31.0169491525424, Blast_Score=242, Evalue=5e-64,
Organism=Drosophila melanogaster, GI24642057, Length=599, Percent_Identity=28.7145242070117, Blast_Score=168, Evalue=7e-42,
Organism=Drosophila melanogaster, GI24645930, Length=585, Percent_Identity=27.5213675213675, Blast_Score=147, Evalue=2e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012132
- InterPro:   IPR000172
- InterPro:   IPR007867 [H]

Pfam domain/function: PF05199 GMC_oxred_C; PF00732 GMC_oxred_N [H]

EC number: 1.1.99.1 [C]

Molecular weight: Translated: 59534; Mature: 59534

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: PS00624 GMC_OXRED_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELDGNFDYVIVGGGSAGCVLASRLTENPDISVCLLEYGGEGKDLAVRVPAGLILMVPGK
CCCCCCCCEEEEECCCCCHHEEHHHCCCCCEEEEEEEECCCCCEEEEECCCCEEEEECCC
PLKLNNWCFHTTPQIHLNNRRGFQPRGQCLGGSSAINAMIYTRGSALDYERWVEQGCTGW
CEEECCEEEECCCEEEECCCCCCCCCCCCCCCCHHEEEEEEECCCCCCHHHHHHCCCCCC
GFDDVLPYFIKSENNIHGADELHGDSGPLHVSDLLSPRDISKAFVEAAIANGLDQNDDFN
CHHHHHHHHCCCCCCCCCHHHCCCCCCCEEHHHCCCCHHHHHHHHHHHHHCCCCCCCCCC
GKKQDGAGLYQVTHFHGEKQGQRCSAAAAYLHPVQSRPNLTVITHAQADRVIFEDKQATG
CCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEECCCCCE
VIYEKDGVEHTVMARHEVILSGGTFGSPKVLMLSGIGPAEHLQSHGIEVLVDAPDVGGNL
EEEECCCCCEEEEEEEEEEEECCCCCCCCEEEEECCCHHHHHHHCCCEEEEECCCCCCCC
QDHLDVVFDYEVNTTDVIGIGIATISTLTKSIRQWRKDGTGLLSTNYAEAGAFFSVGDDP
CCCEEEEEEEECCCCEEEEEHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCEEECCCCH
QEWPNTQLHFVISRVIEHGRDLRRGFAVSCHTCYLRPESRGTVRLDSANPSDAVLIDPNY
HHCCCCHHHHHHHHHHHCCHHHHCCCEEEEEEEEEEECCCCEEEECCCCCCCEEEECCCC
LSHPKDVEYMVAGAERTRAIMQESPMAEYITEDYPAPYIEKDGMLGYIRNKSDTIYHPVG
CCCCCCCEEEEECCHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCEEEEECCCCEEEECCC
TCRMGSDSDSVVDLELKVRGVNGLRVIDASVMPTLISANTNAPTIMIAEKIADLIKANHV
EEECCCCCCCEEEEEEEEECCCCEEEEECCHHCCEEECCCCCCEEEEHHHHHHHHHCCCC
HAESVPA
CCCCCCC
>Mature Secondary Structure
MELDGNFDYVIVGGGSAGCVLASRLTENPDISVCLLEYGGEGKDLAVRVPAGLILMVPGK
CCCCCCCCEEEEECCCCCHHEEHHHCCCCCEEEEEEEECCCCCEEEEECCCCEEEEECCC
PLKLNNWCFHTTPQIHLNNRRGFQPRGQCLGGSSAINAMIYTRGSALDYERWVEQGCTGW
CEEECCEEEECCCEEEECCCCCCCCCCCCCCCCHHEEEEEEECCCCCCHHHHHHCCCCCC
GFDDVLPYFIKSENNIHGADELHGDSGPLHVSDLLSPRDISKAFVEAAIANGLDQNDDFN
CHHHHHHHHCCCCCCCCCHHHCCCCCCCEEHHHCCCCHHHHHHHHHHHHHCCCCCCCCCC
GKKQDGAGLYQVTHFHGEKQGQRCSAAAAYLHPVQSRPNLTVITHAQADRVIFEDKQATG
CCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEECCCCCE
VIYEKDGVEHTVMARHEVILSGGTFGSPKVLMLSGIGPAEHLQSHGIEVLVDAPDVGGNL
EEEECCCCCEEEEEEEEEEEECCCCCCCCEEEEECCCHHHHHHHCCCEEEEECCCCCCCC
QDHLDVVFDYEVNTTDVIGIGIATISTLTKSIRQWRKDGTGLLSTNYAEAGAFFSVGDDP
CCCEEEEEEEECCCCEEEEEHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCEEECCCCH
QEWPNTQLHFVISRVIEHGRDLRRGFAVSCHTCYLRPESRGTVRLDSANPSDAVLIDPNY
HHCCCCHHHHHHHHHHHCCHHHHCCCEEEEEEEEEEECCCCEEEECCCCCCCEEEECCCC
LSHPKDVEYMVAGAERTRAIMQESPMAEYITEDYPAPYIEKDGMLGYIRNKSDTIYHPVG
CCCCCCCEEEEECCHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCEEEEECCCCEEEECCC
TCRMGSDSDSVVDLELKVRGVNGLRVIDASVMPTLISANTNAPTIMIAEKIADLIKANHV
EEECCCCCCCEEEEEEEEECCCCEEEEECCHHCCEEECCCCCCEEEEHHHHHHHHHCCCC
HAESVPA
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Coenzyme Q [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 7 {choline}} 1.1 {phenazine}} [C]

Substrates: Choline; Acceptor [C]

Specific reaction: Choline + Acceptor = Betaine Aldehyde + Reduced Acceptor. [C]

General reaction: Redox reaction [C]

Inhibitor: 2-Dimethylaminoethanol; Betaine aldehyde; Monoethanolamine; Semicarbazide [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1453953 [H]