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The map label for this gene is oprM [H]

Identifier: 93006583

GI number: 93006583

Start: 2177288

End: 2179090

Strand: Direct

Name: oprM [H]

Synonym: Pcryo_1759

Alternate gene names: 93006583

Gene position: 2177288-2179090 (Clockwise)

Preceding gene: 93006582

Following gene: 93006584

Centisome position: 71.16

GC content: 45.98

Gene sequence:

>1803_bases
ATGAGTTTACGTTATTTTGTTACCCCCAACTCAGTAACTAAGGCAGTCTCTTTGACTGCCCGCGTTCGCCAAAATAGTGG
ACGTTTAATAGGTCTAACAGCCTTAGCGATGAGCATGGCGGCTTGTAATACCATTCCAAAGGCTGATACACGTCCTGTAT
TGGCGGTGCCTAATATTCCGATCGAACAAACCTATGGCGCGTTTGATAAAGAGACCGTTAGTAGCGCTGAGCAAGCCAGT
CTTGCTAGTCAACGCTGGCAGAACTTTTACAGCGATGAGCGTTTAAAGGGTCTGATTGCTCTAGGTTTAGAGAACAATAA
AGACTTTGAAAGTGCTCGTTTAGCGATTGAAAAAGCCCGTGCACAATATCAAATTACTGATATCAATGATCTACCTCGCA
TTGATGGTAATGCTGGCTATTCGCGCACGCGTCAACGCGGACAAACAGGCGATAACTATAATGTTAACCTTGGTCTTGCC
AATTATGAGCTCGACTTTTGGGGTAAAATTGCCAGCTTAAAAGATCAAGCGTTGCAGAACTTTTTATCAACGACAGCGGC
AAAAGACGCGACCCAGATCAGTCTGATTAGCAACATTGCTCAAAGCTATGCCAATTTGAGCTATAGCTTGGCGCAGCTAC
AACTGGCAGAGGCGACTGTAGAGAGCCGTGAGAAATCGCTATTTATTGCTAGCAAACGCTTTGAAGCTGGTATTGATCCT
AAGCTGCCCTCTTTGCAATCTAGCGCCTCACTTGAAAATGCCCGACTTGCCGTTTTACGTGCCCAAAGCAGTATTTTAAA
ATCACGTAATGCATTGCAGTTTTTAGTTGGTGGACCGATTCCAACCAATCTGATTCCAACGCCTGCGGTCAGTAATATCA
CAAGTCAGCAAGTATTTAGTGCTGGCTTGCCAAGTGAGCTGCTACGCTATCGTCCTGATGTGTTGCAAGCAGAATATAAT
CTGAAAGCAGCTGGTGCGAATATCGAAGTGGCGCGCGCGTCTTATTACCCCTCTATCAGCTTGGCCAGTAATATTGGCTT
AAGCAGCGGCAGTTTAAATGACCTGTTTAAAAGTGGTTCTGTCGGCTGGTCATTCGGTCCAAGCGTTAGTGTGCCTATCT
TTGATGCTGGTCGCTTAGATGCCAACTATGATGTGGCACAAATCGAGCGTAAGCAGACGCTTGCAGGCTATGAGCGCTCA
ATTCAAACCGCTTTCCGTGAAGTGTCGGATGTCTTAGCCACCCGTGCAACTTTGGGCGAGCAGTTAGAGGGGCAGTATCG
TTTGCAAGACAACTTTGAGCAAACGTATCAGATTGCTGATGCGCGCTTTAAAGCAGGTATCTCAAACTACCTAGACGTCC
TTGATGCTCAGCGTTCGTTATTTTCAACGCAACAAGGCATTTTGGATTTAGAGCTACAGAAAATCATCAGTCAAGTTGAG
CTTTATCAAGTGTTGGGTGGCGGTGCCAATCTAGATGTACCAACCGTGATTCCTGTACCGCACCATAGAAATCTGGTACA
AGTAGTCAATGCAGCTGCCAATAGTAACAAGGCAAAAGCTACTGATGCTATCGATGCGGCAAGTTCTGCACGTGTGGTTT
CTGCTCAAGAAGCCCTCGCCATTAAACAGGCACAGCCAGTAGAAAAATCGACCTTTAAGCCAACGGCTGTGGTCGATGTC
AATAACGATGGCAAGACTGATGCTGCTCTCGGGGTGATTACTGAAGAAACCATCTCAAAAGAGACTCGCATTAAAGAAGT
CAGTGTTGAGCAAGTACCAGTCACGCAGATTATTGAGCCATAA

Upstream 100 bases:

>100_bases
CAGAGTATAATGAACCACCGATCACACACGTTGACGACGATATGCAAAACATGACGCTTTCTAAGAGCGCTCAGTCTGCA
AGCTTTGGAGATAATACACA

Downstream 100 bases:

>100_bases
ATCTTTATAAAGCCCTAAGCGCGTGACGTACTTTTATCGCGTTGATAGTCGATAACTACAATCAGCCCTACCGACTCATT
ATAGTAAGTCGGTAGGGCTT

Product: RND efflux system outer membrane lipoprotein NodT

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 600; Mature: 599

Protein sequence:

>600_residues
MSLRYFVTPNSVTKAVSLTARVRQNSGRLIGLTALAMSMAACNTIPKADTRPVLAVPNIPIEQTYGAFDKETVSSAEQAS
LASQRWQNFYSDERLKGLIALGLENNKDFESARLAIEKARAQYQITDINDLPRIDGNAGYSRTRQRGQTGDNYNVNLGLA
NYELDFWGKIASLKDQALQNFLSTTAAKDATQISLISNIAQSYANLSYSLAQLQLAEATVESREKSLFIASKRFEAGIDP
KLPSLQSSASLENARLAVLRAQSSILKSRNALQFLVGGPIPTNLIPTPAVSNITSQQVFSAGLPSELLRYRPDVLQAEYN
LKAAGANIEVARASYYPSISLASNIGLSSGSLNDLFKSGSVGWSFGPSVSVPIFDAGRLDANYDVAQIERKQTLAGYERS
IQTAFREVSDVLATRATLGEQLEGQYRLQDNFEQTYQIADARFKAGISNYLDVLDAQRSLFSTQQGILDLELQKIISQVE
LYQVLGGGANLDVPTVIPVPHHRNLVQVVNAAANSNKAKATDAIDAASSARVVSAQEALAIKQAQPVEKSTFKPTAVVDV
NNDGKTDAALGVITEETISKETRIKEVSVEQVPVTQIIEP

Sequences:

>Translated_600_residues
MSLRYFVTPNSVTKAVSLTARVRQNSGRLIGLTALAMSMAACNTIPKADTRPVLAVPNIPIEQTYGAFDKETVSSAEQAS
LASQRWQNFYSDERLKGLIALGLENNKDFESARLAIEKARAQYQITDINDLPRIDGNAGYSRTRQRGQTGDNYNVNLGLA
NYELDFWGKIASLKDQALQNFLSTTAAKDATQISLISNIAQSYANLSYSLAQLQLAEATVESREKSLFIASKRFEAGIDP
KLPSLQSSASLENARLAVLRAQSSILKSRNALQFLVGGPIPTNLIPTPAVSNITSQQVFSAGLPSELLRYRPDVLQAEYN
LKAAGANIEVARASYYPSISLASNIGLSSGSLNDLFKSGSVGWSFGPSVSVPIFDAGRLDANYDVAQIERKQTLAGYERS
IQTAFREVSDVLATRATLGEQLEGQYRLQDNFEQTYQIADARFKAGISNYLDVLDAQRSLFSTQQGILDLELQKIISQVE
LYQVLGGGANLDVPTVIPVPHHRNLVQVVNAAANSNKAKATDAIDAASSARVVSAQEALAIKQAQPVEKSTFKPTAVVDV
NNDGKTDAALGVITEETISKETRIKEVSVEQVPVTQIIEP
>Mature_599_residues
SLRYFVTPNSVTKAVSLTARVRQNSGRLIGLTALAMSMAACNTIPKADTRPVLAVPNIPIEQTYGAFDKETVSSAEQASL
ASQRWQNFYSDERLKGLIALGLENNKDFESARLAIEKARAQYQITDINDLPRIDGNAGYSRTRQRGQTGDNYNVNLGLAN
YELDFWGKIASLKDQALQNFLSTTAAKDATQISLISNIAQSYANLSYSLAQLQLAEATVESREKSLFIASKRFEAGIDPK
LPSLQSSASLENARLAVLRAQSSILKSRNALQFLVGGPIPTNLIPTPAVSNITSQQVFSAGLPSELLRYRPDVLQAEYNL
KAAGANIEVARASYYPSISLASNIGLSSGSLNDLFKSGSVGWSFGPSVSVPIFDAGRLDANYDVAQIERKQTLAGYERSI
QTAFREVSDVLATRATLGEQLEGQYRLQDNFEQTYQIADARFKAGISNYLDVLDAQRSLFSTQQGILDLELQKIISQVEL
YQVLGGGANLDVPTVIPVPHHRNLVQVVNAAANSNKAKATDAIDAASSARVVSAQEALAIKQAQPVEKSTFKPTAVVDVN
NDGKTDAALGVITEETISKETRIKEVSVEQVPVTQIIEP

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=460, Percent_Identity=37.1739130434783, Blast_Score=293, Evalue=2e-80,
Organism=Escherichia coli, GI2367348, Length=445, Percent_Identity=25.6179775280899, Blast_Score=122, Evalue=5e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 65030; Mature: 64899

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
0.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
0.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLRYFVTPNSVTKAVSLTARVRQNSGRLIGLTALAMSMAACNTIPKADTRPVLAVPNIP
CCEEEEECCCHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHCCCCCCCCCCEEECCCCC
IEQTYGAFDKETVSSAEQASLASQRWQNFYSDERLKGLIALGLENNKDFESARLAIEKAR
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHCEEEEEECCCCCCHHHHHHHHHHHH
AQYQITDINDLPRIDGNAGYSRTRQRGQTGDNYNVNLGLANYELDFWGKIASLKDQALQN
CEEEEECCCCCCCCCCCCCCHHHHHCCCCCCCEEEEEEEEEEEHHHHHHHHHHHHHHHHH
FLSTTAAKDATQISLISNIAQSYANLSYSLAQLQLAEATVESREKSLFIASKRFEAGIDP
HHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCEEEEEEHHHCCCCCC
KLPSLQSSASLENARLAVLRAQSSILKSRNALQFLVGGPIPTNLIPTPAVSNITSQQVFS
CCCCCCCCCCCCHHHEEEHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHH
AGLPSELLRYRPDVLQAEYNLKAAGANIEVARASYYPSISLASNIGLSSGSLNDLFKSGS
CCCCHHHHHCCCCHHHCCCCEEECCCCEEEEECCCCCCEEHHHCCCCCCCCHHHHHHCCC
VGWSFGPSVSVPIFDAGRLDANYDVAQIERKQTLAGYERSIQTAFREVSDVLATRATLGE
CCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QLEGQYRLQDNFEQTYQIADARFKAGISNYLDVLDAQRSLFSTQQGILDLELQKIISQVE
HHCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
LYQVLGGGANLDVPTVIPVPHHRNLVQVVNAAANSNKAKATDAIDAASSARVVSAQEALA
HHHHHCCCCCCCCCEEECCCCCCCHHHHHHHHCCCCCCCHHHHHHCCCCCHHHHHHHHHH
IKQAQPVEKSTFKPTAVVDVNNDGKTDAALGVITEETISKETRIKEVSVEQVPVTQIIEP
HHHCCCCCCCCCCCEEEEECCCCCCCCHHEEEHHHHHHHHHHHHHHCCCCCCCHHHHCCC
>Mature Secondary Structure 
SLRYFVTPNSVTKAVSLTARVRQNSGRLIGLTALAMSMAACNTIPKADTRPVLAVPNIP
CEEEEECCCHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHCCCCCCCCCCEEECCCCC
IEQTYGAFDKETVSSAEQASLASQRWQNFYSDERLKGLIALGLENNKDFESARLAIEKAR
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHCEEEEEECCCCCCHHHHHHHHHHHH
AQYQITDINDLPRIDGNAGYSRTRQRGQTGDNYNVNLGLANYELDFWGKIASLKDQALQN
CEEEEECCCCCCCCCCCCCCHHHHHCCCCCCCEEEEEEEEEEEHHHHHHHHHHHHHHHHH
FLSTTAAKDATQISLISNIAQSYANLSYSLAQLQLAEATVESREKSLFIASKRFEAGIDP
HHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCEEEEEEHHHCCCCCC
KLPSLQSSASLENARLAVLRAQSSILKSRNALQFLVGGPIPTNLIPTPAVSNITSQQVFS
CCCCCCCCCCCCHHHEEEHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHH
AGLPSELLRYRPDVLQAEYNLKAAGANIEVARASYYPSISLASNIGLSSGSLNDLFKSGS
CCCCHHHHHCCCCHHHCCCCEEECCCCEEEEECCCCCCEEHHHCCCCCCCCHHHHHHCCC
VGWSFGPSVSVPIFDAGRLDANYDVAQIERKQTLAGYERSIQTAFREVSDVLATRATLGE
CCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QLEGQYRLQDNFEQTYQIADARFKAGISNYLDVLDAQRSLFSTQQGILDLELQKIISQVE
HHCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
LYQVLGGGANLDVPTVIPVPHHRNLVQVVNAAANSNKAKATDAIDAASSARVVSAQEALA
HHHHHCCCCCCCCCEEECCCCCCCHHHHHHHHCCCCCCCHHHHHHCCCCCHHHHHHHHHH
IKQAQPVEKSTFKPTAVVDVNNDGKTDAALGVITEETISKETRIKEVSVEQVPVTQIIEP
HHHCCCCCCCCCCCEEEEECCCCCCCCHHEEEHHHHHHHHHHHHHHCCCCCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]