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The map label for this gene is rsmD [H]

Identifier: 93006570

GI number: 93006570

Start: 2162811

End: 2163497

Strand: Direct

Name: rsmD [H]

Synonym: Pcryo_1746

Alternate gene names: 93006570

Gene position: 2162811-2163497 (Clockwise)

Preceding gene: 93006569

Following gene: 93006571

Centisome position: 70.68

GC content: 41.92

Gene sequence:

>687_bases
ATGAAAAAATCTTCAAGCGCGCGTAAGCCTAGTAAAAACCAAAAAACCAAATCTACTGCAGCTGGTGATGTACGTATTAT
CGGTGGTCAGTTTAAACGCCGTATCGTACGTTTTATTGATGCAGAAGGATTACGTCCGACCCCAGACCGGCTACGTGAGA
CCTTATTCAATTGGCTGATTTCTGATATTCATGACGCTCAAGTGCTCGATAGCTGCGCCGGTAGCGGCGTCTTAGGTTTT
GAAGCCTTATCGCGCGGTGCTGCTCATTGTATTTTTATCGAAATGAATGCCGCCCAAACTCAAATGCTACGCCAAAGTGC
CGAGATGTTACGTCTTGATAGTGACAGCCATAAAATCATTCAGGGTACAGCCGAACAAGTTCTACAACAAAAGCAGCTTG
TAACGCGTCCCTTTGATATTGTTTTTATAGATCCACCTTATGCTAAGGATTTATGGCAATCTATTTTGACCACTTTGTTT
ACCCAAGATCTAATCAATACAGACACGCTTATCTACCTAGAAGCGGATAAAGATTTAGATGAGCAATTGAGCCAACTGGC
AACGGTTTTTAATACCACGCTAGCGCTGCAAAAAAACGATGAATCACAAACATTGTATTTTGATTGTCTGAAACAGACCA
AAGTTGGACAAGTTGTTGCTGGACTTTATCGCCTATTATCGTCATAA

Upstream 100 bases:

>100_bases
CAAAGCACTAGAAGCCGCAAAAGAACAGGCTCAAACCGCTACAACGACAGCGACTTCTGACTAACATATTCAGTATAGAG
TAGATAACTAAGCTAATTTT

Downstream 100 bases:

>100_bases
TTGTCCGGTTAATGAATTCTGTTAATAAAATTTATAAATGTTTAAAACCGCTAAAAAACTGGCCAACGCTGCAGTTTAAT
GTATATAAGGCGAAAATAAA

Product: hypothetical protein

Products: NA

Alternate protein names: 16S rRNA m2G966 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase [H]

Number of amino acids: Translated: 228; Mature: 228

Protein sequence:

>228_residues
MKKSSSARKPSKNQKTKSTAAGDVRIIGGQFKRRIVRFIDAEGLRPTPDRLRETLFNWLISDIHDAQVLDSCAGSGVLGF
EALSRGAAHCIFIEMNAAQTQMLRQSAEMLRLDSDSHKIIQGTAEQVLQQKQLVTRPFDIVFIDPPYAKDLWQSILTTLF
TQDLINTDTLIYLEADKDLDEQLSQLATVFNTTLALQKNDESQTLYFDCLKQTKVGQVVAGLYRLLSS

Sequences:

>Translated_228_residues
MKKSSSARKPSKNQKTKSTAAGDVRIIGGQFKRRIVRFIDAEGLRPTPDRLRETLFNWLISDIHDAQVLDSCAGSGVLGF
EALSRGAAHCIFIEMNAAQTQMLRQSAEMLRLDSDSHKIIQGTAEQVLQQKQLVTRPFDIVFIDPPYAKDLWQSILTTLF
TQDLINTDTLIYLEADKDLDEQLSQLATVFNTTLALQKNDESQTLYFDCLKQTKVGQVVAGLYRLLSS
>Mature_228_residues
MKKSSSARKPSKNQKTKSTAAGDVRIIGGQFKRRIVRFIDAEGLRPTPDRLRETLFNWLISDIHDAQVLDSCAGSGVLGF
EALSRGAAHCIFIEMNAAQTQMLRQSAEMLRLDSDSHKIIQGTAEQVLQQKQLVTRPFDIVFIDPPYAKDLWQSILTTLF
TQDLINTDTLIYLEADKDLDEQLSQLATVFNTTLALQKNDESQTLYFDCLKQTKVGQVVAGLYRLLSS

Specific function: Specifically methylates the guanosine in position 966 of 16S rRNA in the assembled 30S particle [H]

COG id: COG0742

COG function: function code L; N6-adenine-specific methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmD family [H]

Homologues:

Organism=Escherichia coli, GI1789875, Length=163, Percent_Identity=41.7177914110429, Blast_Score=127, Evalue=7e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004398 [H]

Pfam domain/function: PF03602 Cons_hypoth95 [H]

EC number: =2.1.1.171 [H]

Molecular weight: Translated: 25584; Mature: 25584

Theoretical pI: Translated: 7.44; Mature: 7.44

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKSSSARKPSKNQKTKSTAAGDVRIIGGQFKRRIVRFIDAEGLRPTPDRLRETLFNWLI
CCCCCCCCCCCCCCCHHHCCCCCEEEECHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
SDIHDAQVLDSCAGSGVLGFEALSRGAAHCIFIEMNAAQTQMLRQSAEMLRLDSDSHKII
HHHHHHHHHHHHCCCCCHHHHHHHCCCCEEEEEEECHHHHHHHHHHHHHHHCCCCCHHHH
QGTAEQVLQQKQLVTRPFDIVFIDPPYAKDLWQSILTTLFTQDLINTDTLIYLEADKDLD
HHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHH
EQLSQLATVFNTTLALQKNDESQTLYFDCLKQTKVGQVVAGLYRLLSS
HHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKKSSSARKPSKNQKTKSTAAGDVRIIGGQFKRRIVRFIDAEGLRPTPDRLRETLFNWLI
CCCCCCCCCCCCCCCHHHCCCCCEEEECHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
SDIHDAQVLDSCAGSGVLGFEALSRGAAHCIFIEMNAAQTQMLRQSAEMLRLDSDSHKII
HHHHHHHHHHHHCCCCCHHHHHHHCCCCEEEEEEECHHHHHHHHHHHHHHHCCCCCHHHH
QGTAEQVLQQKQLVTRPFDIVFIDPPYAKDLWQSILTTLFTQDLINTDTLIYLEADKDLD
HHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHH
EQLSQLATVFNTTLALQKNDESQTLYFDCLKQTKVGQVVAGLYRLLSS
HHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]