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The map label for this gene is iscS [H]

Identifier: 93006480

GI number: 93006480

Start: 2040132

End: 2041358

Strand: Direct

Name: iscS [H]

Synonym: Pcryo_1656

Alternate gene names: 93006480

Gene position: 2040132-2041358 (Clockwise)

Preceding gene: 93006479

Following gene: 93006481

Centisome position: 66.67

GC content: 44.58

Gene sequence:

>1227_bases
ATGAGCCAACATAACACCCTTATATATTTAGATTATGCCGCCACGACGCCAGTTGCTAAGTCAGTCGCTGCCAAGATGAG
CGAATATCTGACGGTAGATGGCATCTTTGGTAACCCAGCATCACGCTCGCATGGCTACGGCTGGCAAGCAGAAGAGGCGG
TAGAAACGGCTCGTCAGCAAGTTGCTGAAGTCATCAATGCTGATCCTCGCGAAATCGTCTTTACCTCTGGTGCGACAGAA
TCTGATAACTTAGCCATCAAAGGTGCTGCTCATTTTTATCAGTCTCGTGGTAAGCATATTATTACCAGTAAAATTGAGCA
CAAAGCGGTATTAGATACCTGCCGTGAACTTGAGCAAGAAGGTTTTGAAATTACGTATCTTGAGCCGCAGCCAAGCACTG
GCATGATTTTACCAGAGCAAGTAAAAGAAGCTCTGCGTGAAGATACTATCTTAGTGTCTCTCATGATGGTGAATAATGAG
CTTGGTACAATTACTGATGTTGCAGCGATTGGTGAAATCACACGTGAAGCGGGTGTTGTTTTTCACGTTGATGGCGCACA
GTCTGTTGGTAAAGTACTTATCAATCTTGCAGAGATGAAAATTGACCTAATGAGTTTCTCAGGTCATAAGGCTTATGGTC
CTAAAGGTATTGGTGCTCTATTCGTTAGTCGTAAGCCACGTATTCGCCTAAAAGCCGAGCAGCATGGTGGTGGTCATGAG
CGCGGTATGCGTTCAGGTACTTTGCCGACTCACCAGATCGTAGGTCTTGGCGCAGCATTTGAGTTAGCTAATCAGCGCTA
TCAAGAAGACCATGCCCATGCGGCAAAATTACGCCAAAAACTGTGGGATGGTCTACAAGATATCGAAGAGATTTATCTAA
ACGGTGATTTAGAAAACAGCGTACCTAATATTGTAAATATCAGCTTTAACTTTGTTGAAGGCGAATCCCTGATGATGTCG
CTAAAAGACTTAGCTGTCTCATCAGGTTCAGCTTGTACGTCAGCGACTCTTGAGCCATCTTATGTCTTGCGCGCTATTGG
TCGCCCAGACGAGTTGGCACATAGCTCAATCAGATTTAGCTTTGGTCGCTACACTACTGAAGAAGACATTGATACGGTTA
TCACTCAGATGCATCAAGCTGTTGATAAATTACGTGCGCTATCGCCACTTTGGGATATGTATCAAGAAGGTGTTGATTTA
GACTCAGTAGAATGGTCTGAGCACTAA

Upstream 100 bases:

>100_bases
TGCAGTCTGTATCAGATCGGCAGCACAGCGATGCTCTAAAAGATATTTCTATAAAAGACATTAACACTATTACTTTATCG
ACCAGCGAGGCAAACCCAGC

Downstream 100 bases:

>100_bases
TACTACATTTAAGCTTTATAATTTAAAAAATAATGGCAACAGCGATATTTTTATTTACTTTTTCTACTAAACATAAAAAT
ATCGCCATAGCTAATGAATC

Product: cysteine desulfurase IscS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 408; Mature: 407

Protein sequence:

>408_residues
MSQHNTLIYLDYAATTPVAKSVAAKMSEYLTVDGIFGNPASRSHGYGWQAEEAVETARQQVAEVINADPREIVFTSGATE
SDNLAIKGAAHFYQSRGKHIITSKIEHKAVLDTCRELEQEGFEITYLEPQPSTGMILPEQVKEALREDTILVSLMMVNNE
LGTITDVAAIGEITREAGVVFHVDGAQSVGKVLINLAEMKIDLMSFSGHKAYGPKGIGALFVSRKPRIRLKAEQHGGGHE
RGMRSGTLPTHQIVGLGAAFELANQRYQEDHAHAAKLRQKLWDGLQDIEEIYLNGDLENSVPNIVNISFNFVEGESLMMS
LKDLAVSSGSACTSATLEPSYVLRAIGRPDELAHSSIRFSFGRYTTEEDIDTVITQMHQAVDKLRALSPLWDMYQEGVDL
DSVEWSEH

Sequences:

>Translated_408_residues
MSQHNTLIYLDYAATTPVAKSVAAKMSEYLTVDGIFGNPASRSHGYGWQAEEAVETARQQVAEVINADPREIVFTSGATE
SDNLAIKGAAHFYQSRGKHIITSKIEHKAVLDTCRELEQEGFEITYLEPQPSTGMILPEQVKEALREDTILVSLMMVNNE
LGTITDVAAIGEITREAGVVFHVDGAQSVGKVLINLAEMKIDLMSFSGHKAYGPKGIGALFVSRKPRIRLKAEQHGGGHE
RGMRSGTLPTHQIVGLGAAFELANQRYQEDHAHAAKLRQKLWDGLQDIEEIYLNGDLENSVPNIVNISFNFVEGESLMMS
LKDLAVSSGSACTSATLEPSYVLRAIGRPDELAHSSIRFSFGRYTTEEDIDTVITQMHQAVDKLRALSPLWDMYQEGVDL
DSVEWSEH
>Mature_407_residues
SQHNTLIYLDYAATTPVAKSVAAKMSEYLTVDGIFGNPASRSHGYGWQAEEAVETARQQVAEVINADPREIVFTSGATES
DNLAIKGAAHFYQSRGKHIITSKIEHKAVLDTCRELEQEGFEITYLEPQPSTGMILPEQVKEALREDTILVSLMMVNNEL
GTITDVAAIGEITREAGVVFHVDGAQSVGKVLINLAEMKIDLMSFSGHKAYGPKGIGALFVSRKPRIRLKAEQHGGGHER
GMRSGTLPTHQIVGLGAAFELANQRYQEDHAHAAKLRQKLWDGLQDIEEIYLNGDLENSVPNIVNISFNFVEGESLMMSL
KDLAVSSGSACTSATLEPSYVLRAIGRPDELAHSSIRFSFGRYTTEEDIDTVITQMHQAVDKLRALSPLWDMYQEGVDLD
SVEWSEH

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=402, Percent_Identity=54.9751243781095, Blast_Score=474, Evalue=1e-134,
Organism=Homo sapiens, GI156713448, Length=422, Percent_Identity=31.7535545023697, Blast_Score=183, Evalue=3e-46,
Organism=Escherichia coli, GI48994898, Length=401, Percent_Identity=67.0822942643392, Blast_Score=592, Evalue=1e-170,
Organism=Escherichia coli, GI1789175, Length=400, Percent_Identity=26, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1787970, Length=412, Percent_Identity=26.6990291262136, Blast_Score=91, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI25143064, Length=403, Percent_Identity=54.8387096774194, Blast_Score=454, Evalue=1e-128,
Organism=Caenorhabditis elegans, GI17533177, Length=329, Percent_Identity=33.434650455927, Blast_Score=160, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6319831, Length=401, Percent_Identity=52.1197007481297, Blast_Score=438, Evalue=1e-124,
Organism=Drosophila melanogaster, GI20129463, Length=402, Percent_Identity=53.2338308457711, Blast_Score=443, Evalue=1e-124,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR010240
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 44932; Mature: 44801

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQHNTLIYLDYAATTPVAKSVAAKMSEYLTVDGIFGNPASRSHGYGWQAEEAVETARQQ
CCCCCEEEEEEECCCCHHHHHHHHHHHHHEEEECCCCCCCCCCCCCCCCHHHHHHHHHHH
VAEVINADPREIVFTSGATESDNLAIKGAAHFYQSRGKHIITSKIEHKAVLDTCRELEQE
HHHHHCCCCCEEEEECCCCCCCCEEEEHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHC
GFEITYLEPQPSTGMILPEQVKEALREDTILVSLMMVNNELGTITDVAAIGEITREAGVV
CCEEEEECCCCCCCCCCHHHHHHHHHHHHHEEEEEEECCCCCCHHHHHHHHHHHHHCCEE
FHVDGAQSVGKVLINLAEMKIDLMSFSGHKAYGPKGIGALFVSRKPRIRLKAEQHGGGHE
EEECCHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCEEEEECCCCCEEEEECCCCCCHH
RGMRSGTLPTHQIVGLGAAFELANQRYQEDHAHAAKLRQKLWDGLQDIEEIYLNGDLENS
CCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
VPNIVNISFNFVEGESLMMSLKDLAVSSGSACTSATLEPSYVLRAIGRPDELAHSSIRFS
CCCEEEEEEEEECCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHEEE
FGRYTTEEDIDTVITQMHQAVDKLRALSPLWDMYQEGVDLDSVEWSEH
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
SQHNTLIYLDYAATTPVAKSVAAKMSEYLTVDGIFGNPASRSHGYGWQAEEAVETARQQ
CCCCEEEEEEECCCCHHHHHHHHHHHHHEEEECCCCCCCCCCCCCCCCHHHHHHHHHHH
VAEVINADPREIVFTSGATESDNLAIKGAAHFYQSRGKHIITSKIEHKAVLDTCRELEQE
HHHHHCCCCCEEEEECCCCCCCCEEEEHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHC
GFEITYLEPQPSTGMILPEQVKEALREDTILVSLMMVNNELGTITDVAAIGEITREAGVV
CCEEEEECCCCCCCCCCHHHHHHHHHHHHHEEEEEEECCCCCCHHHHHHHHHHHHHCCEE
FHVDGAQSVGKVLINLAEMKIDLMSFSGHKAYGPKGIGALFVSRKPRIRLKAEQHGGGHE
EEECCHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCEEEEECCCCCEEEEECCCCCCHH
RGMRSGTLPTHQIVGLGAAFELANQRYQEDHAHAAKLRQKLWDGLQDIEEIYLNGDLENS
CCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
VPNIVNISFNFVEGESLMMSLKDLAVSSGSACTSATLEPSYVLRAIGRPDELAHSSIRFS
CCCEEEEEEEEECCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHEEE
FGRYTTEEDIDTVITQMHQAVDKLRALSPLWDMYQEGVDLDSVEWSEH
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA