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The map label for this gene is purH [H]

Identifier: 93006475

GI number: 93006475

Start: 2033834

End: 2035414

Strand: Direct

Name: purH [H]

Synonym: Pcryo_1651

Alternate gene names: 93006475

Gene position: 2033834-2035414 (Clockwise)

Preceding gene: 93006474

Following gene: 93006478

Centisome position: 66.47

GC content: 47.25

Gene sequence:

>1581_bases
ATGAGTAAAGCCCCACTAGCACTACTGTCGGTCTCCAATAAATCCAATATCGTTGAGTTCGCTCAAGGTCTGATTAAAGC
AGGTTTTGGTCTGTTATCGACTGGCGGTACTTTCCGTTTGCTAACAGAGCATAATGTCGCTGTCACCGAAGTATCAGACT
ATACGGGTTTCCCTGAGATGATGGATGGTCGGGTTAAAACGCTTCATCCCAAGATTCACGGTGGCATTTTGGGACGCCGC
GGCACAGATGATGCGGTGATGAGTGAGCACGCGATTGAGCGTATTGATTTGGTAGTCGTAAACCTTTATCCATTTGCAGA
AACGATTGCCCGTAGTGACGTTACCATGAATGATGCCATCGAAAATATCGATATTGGCGGACCTACGATGGTGCGTTCAG
CGGCAAAGAATCATGCACACGTTGGTATTGTGACTGATCCAGCTGATTATACGCGTGTACTTGAAGCATTAGGAGATGGG
ACGACATTAACCCATGCCTTACGCTACGATTTAGCGGTCAAAGCGTTTGAGCATACGGCACAATACGACGGGATGATTGC
CAACTTCTTAGGCAGCCGTGTTAACGAGAATCAAGAGCCGGAAGATTTTTCACGTACCTTTAACGTTCAGCTAGAAAAAG
TGCAAGACCTGCGCTACGGTGAAAACCCGCATCAAAAAGCAGCGTTCTATGTTGAAAACAACGCTTTAAAAAGCAAGCAA
GCATCTATTGCTACTGCTAAGCAATTGCAAGGCAAAGCATTGTCTTATAACAATATCGCCGATACTGATGCCGCGCTTGA
ATGTGTGAAAGCTTTTAGTACGCCTGCCTGTGTGATTGTCAAGCATGCCAACCCTTGCGGCGTTGCTGTCGACACTGATC
AAGTAGCAGCATATCGCACTGCCTTTAGCACCGATCCTGAATCTTCTTTTGGCGGCATTATCGCTTTTAACCGCCCGTTA
ACACTTGCCGCTGCTAAAGCCATTATCGACAATCAGTTTGTTGAAGTCATTATTGCCCCAAGCATTGAAGACGGTGTCCT
TGAAGCGACAGCTAGCAAGAAAAACGTTCGCGTTTTGGTCTGCGGCGACTTGCCAGCACCTGAGTTACGTGACCGCCAGC
TTGATTATAAGCGCGTCAATGGGGGCTTACTGGTGCAAGAGCAAGATTTAGGGTTAATTACTGCCAATGATTTAAAAATC
GTCACAGACGTACAACCAACTGAAGCACAAATTGCTGATTTACTGTTTAGCTGGAACGTTGCAAAATACGTTAAATCTAA
CGCCATCGTTTATGCTAAAGGTCAGCGTACCATTGGTGTCGGCGCAGGTCAGATGAGCCGTGTTAACTCAGCCCGTATCG
CTGCTATTAAAGCGGAGCACGCCGGACTTGCAACCGAAGGCGCGGTTATGGCATCTGATGCTTTCTTCCCATTCCGTGAT
GGTATCGATAATGCTGCCGAAGTAGGTATCGCAGCGATCATCCAACCAGGTGGCTCTATGCGTGATGATGAAACCATTGC
TGCGGCAAATGAGCACGGTATCGCCATGGTATTCACGGGTATGCGTCATTTCCGTCATTAA

Upstream 100 bases:

>100_bases
TTGTAGTTTATGATCTTATCACTCACGATATGTTCTATACTGATTGTCATTGTTACCTTGCCAGACGTTTACGTCAGGCT
TTTTTTATGGAAATTTTTTT

Downstream 100 bases:

>100_bases
TTCATTATCAATTATTATCAGTATTAAAAATGCTTACCCTTGAATAAAAAAAGCCACTGTATGATTACAGTGGCTTTTTT
AATGGTTAAAAATAGCTTTA

Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase [H]

Number of amino acids: Translated: 526; Mature: 525

Protein sequence:

>526_residues
MSKAPLALLSVSNKSNIVEFAQGLIKAGFGLLSTGGTFRLLTEHNVAVTEVSDYTGFPEMMDGRVKTLHPKIHGGILGRR
GTDDAVMSEHAIERIDLVVVNLYPFAETIARSDVTMNDAIENIDIGGPTMVRSAAKNHAHVGIVTDPADYTRVLEALGDG
TTLTHALRYDLAVKAFEHTAQYDGMIANFLGSRVNENQEPEDFSRTFNVQLEKVQDLRYGENPHQKAAFYVENNALKSKQ
ASIATAKQLQGKALSYNNIADTDAALECVKAFSTPACVIVKHANPCGVAVDTDQVAAYRTAFSTDPESSFGGIIAFNRPL
TLAAAKAIIDNQFVEVIIAPSIEDGVLEATASKKNVRVLVCGDLPAPELRDRQLDYKRVNGGLLVQEQDLGLITANDLKI
VTDVQPTEAQIADLLFSWNVAKYVKSNAIVYAKGQRTIGVGAGQMSRVNSARIAAIKAEHAGLATEGAVMASDAFFPFRD
GIDNAAEVGIAAIIQPGGSMRDDETIAAANEHGIAMVFTGMRHFRH

Sequences:

>Translated_526_residues
MSKAPLALLSVSNKSNIVEFAQGLIKAGFGLLSTGGTFRLLTEHNVAVTEVSDYTGFPEMMDGRVKTLHPKIHGGILGRR
GTDDAVMSEHAIERIDLVVVNLYPFAETIARSDVTMNDAIENIDIGGPTMVRSAAKNHAHVGIVTDPADYTRVLEALGDG
TTLTHALRYDLAVKAFEHTAQYDGMIANFLGSRVNENQEPEDFSRTFNVQLEKVQDLRYGENPHQKAAFYVENNALKSKQ
ASIATAKQLQGKALSYNNIADTDAALECVKAFSTPACVIVKHANPCGVAVDTDQVAAYRTAFSTDPESSFGGIIAFNRPL
TLAAAKAIIDNQFVEVIIAPSIEDGVLEATASKKNVRVLVCGDLPAPELRDRQLDYKRVNGGLLVQEQDLGLITANDLKI
VTDVQPTEAQIADLLFSWNVAKYVKSNAIVYAKGQRTIGVGAGQMSRVNSARIAAIKAEHAGLATEGAVMASDAFFPFRD
GIDNAAEVGIAAIIQPGGSMRDDETIAAANEHGIAMVFTGMRHFRH
>Mature_525_residues
SKAPLALLSVSNKSNIVEFAQGLIKAGFGLLSTGGTFRLLTEHNVAVTEVSDYTGFPEMMDGRVKTLHPKIHGGILGRRG
TDDAVMSEHAIERIDLVVVNLYPFAETIARSDVTMNDAIENIDIGGPTMVRSAAKNHAHVGIVTDPADYTRVLEALGDGT
TLTHALRYDLAVKAFEHTAQYDGMIANFLGSRVNENQEPEDFSRTFNVQLEKVQDLRYGENPHQKAAFYVENNALKSKQA
SIATAKQLQGKALSYNNIADTDAALECVKAFSTPACVIVKHANPCGVAVDTDQVAAYRTAFSTDPESSFGGIIAFNRPLT
LAAAKAIIDNQFVEVIIAPSIEDGVLEATASKKNVRVLVCGDLPAPELRDRQLDYKRVNGGLLVQEQDLGLITANDLKIV
TDVQPTEAQIADLLFSWNVAKYVKSNAIVYAKGQRTIGVGAGQMSRVNSARIAAIKAEHAGLATEGAVMASDAFFPFRDG
IDNAAEVGIAAIIQPGGSMRDDETIAAANEHGIAMVFTGMRHFRH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family [H]

Homologues:

Organism=Homo sapiens, GI20127454, Length=542, Percent_Identity=35.0553505535055, Blast_Score=243, Evalue=3e-64,
Organism=Escherichia coli, GI1790439, Length=526, Percent_Identity=65.0190114068441, Blast_Score=682, Evalue=0.0,
Organism=Caenorhabditis elegans, GI71985564, Length=508, Percent_Identity=33.8582677165354, Blast_Score=248, Evalue=6e-66,
Organism=Caenorhabditis elegans, GI71985574, Length=339, Percent_Identity=28.023598820059, Blast_Score=92, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI71985556, Length=60, Percent_Identity=51.6666666666667, Blast_Score=78, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6323768, Length=492, Percent_Identity=36.1788617886179, Blast_Score=260, Evalue=4e-70,
Organism=Saccharomyces cerevisiae, GI6323056, Length=494, Percent_Identity=35.2226720647773, Blast_Score=257, Evalue=3e-69,
Organism=Drosophila melanogaster, GI24649832, Length=514, Percent_Identity=34.8249027237354, Blast_Score=248, Evalue=1e-65,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607 [H]

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS [H]

EC number: =2.1.2.3; =3.5.4.10 [H]

Molecular weight: Translated: 56555; Mature: 56424

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKAPLALLSVSNKSNIVEFAQGLIKAGFGLLSTGGTFRLLTEHNVAVTEVSDYTGFPEM
CCCCCEEEEEECCCCHHHHHHHHHHHCCCCEEECCCEEEEEEECCEEEEEECCCCCCHHH
MDGRVKTLHPKIHGGILGRRGTDDAVMSEHAIERIDLVVVNLYPFAETIARSDVTMNDAI
HCCCEEEECCHHHCCCCCCCCCCHHHHHHHHHHEEEEEEEEEEHHHHHHHHCCCCHHHHH
ENIDIGGPTMVRSAAKNHAHVGIVTDPADYTRVLEALGDGTTLTHALRYDLAVKAFEHTA
HCCCCCCHHHHHHHHCCCCEEEEEECCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
QYDGMIANFLGSRVNENQEPEDFSRTFNVQLEKVQDLRYGENPHQKAAFYVENNALKSKQ
HHCCHHHHHHHHHCCCCCCCHHHHHEECEEHHHHHHHHCCCCCCCCEEEEEECCCCCCHH
ASIATAKQLQGKALSYNNIADTDAALECVKAFSTPACVIVKHANPCGVAVDTDQVAAYRT
HHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCEEECCHHHHHHHH
AFSTDPESSFGGIIAFNRPLTLAAAKAIIDNQFVEVIIAPSIEDGVLEATASKKNVRVLV
HCCCCCCCCCCCEEEECCCHHHHHHHHHHCCCEEEEEEECCCCCCCEEECCCCCCEEEEE
CGDLPAPELRDRQLDYKRVNGGLLVQEQDLGLITANDLKIVTDVQPTEAQIADLLFSWNV
ECCCCCCCHHCCCCCCEECCCCEEEEECCCCEEEECCEEEEECCCCCHHHHHHHHHHCCH
AKYVKSNAIVYAKGQRTIGVGAGQMSRVNSARIAAIKAEHAGLATEGAVMASDAFFPFRD
HHHHHCCEEEEECCCEEEEECCCCHHHCCCCEEEEEEECCCCCCCCCCEEECCCCCCHHC
GIDNAAEVGIAAIIQPGGSMRDDETIAAANEHGIAMVFTGMRHFRH
CCCHHHHCCEEEEECCCCCCCCCCEEEECCCCCEEEEEECHHHHCC
>Mature Secondary Structure 
SKAPLALLSVSNKSNIVEFAQGLIKAGFGLLSTGGTFRLLTEHNVAVTEVSDYTGFPEM
CCCCEEEEEECCCCHHHHHHHHHHHCCCCEEECCCEEEEEEECCEEEEEECCCCCCHHH
MDGRVKTLHPKIHGGILGRRGTDDAVMSEHAIERIDLVVVNLYPFAETIARSDVTMNDAI
HCCCEEEECCHHHCCCCCCCCCCHHHHHHHHHHEEEEEEEEEEHHHHHHHHCCCCHHHHH
ENIDIGGPTMVRSAAKNHAHVGIVTDPADYTRVLEALGDGTTLTHALRYDLAVKAFEHTA
HCCCCCCHHHHHHHHCCCCEEEEEECCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
QYDGMIANFLGSRVNENQEPEDFSRTFNVQLEKVQDLRYGENPHQKAAFYVENNALKSKQ
HHCCHHHHHHHHHCCCCCCCHHHHHEECEEHHHHHHHHCCCCCCCCEEEEEECCCCCCHH
ASIATAKQLQGKALSYNNIADTDAALECVKAFSTPACVIVKHANPCGVAVDTDQVAAYRT
HHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCEEECCHHHHHHHH
AFSTDPESSFGGIIAFNRPLTLAAAKAIIDNQFVEVIIAPSIEDGVLEATASKKNVRVLV
HCCCCCCCCCCCEEEECCCHHHHHHHHHHCCCEEEEEEECCCCCCCEEECCCCCCEEEEE
CGDLPAPELRDRQLDYKRVNGGLLVQEQDLGLITANDLKIVTDVQPTEAQIADLLFSWNV
ECCCCCCCHHCCCCCCEECCCCEEEEECCCCEEEECCEEEEECCCCCHHHHHHHHHHCCH
AKYVKSNAIVYAKGQRTIGVGAGQMSRVNSARIAAIKAEHAGLATEGAVMASDAFFPFRD
HHHHHCCEEEEECCCEEEEECCCCHHHCCCCEEEEEEECCCCCCCCCCEEECCCCCCHHC
GIDNAAEVGIAAIIQPGGSMRDDETIAAANEHGIAMVFTGMRHFRH
CCCHHHHCCEEEEECCCCCCCCCCEEEECCCCCEEEEEECHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA