| Definition | Psychrobacter cryohalolentis K5 chromosome, complete genome. |
|---|---|
| Accession | NC_007969 |
| Length | 3,059,876 |
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The map label for this gene is mrcB [H]
Identifier: 93006470
GI number: 93006470
Start: 2027215
End: 2029698
Strand: Direct
Name: mrcB [H]
Synonym: Pcryo_1646
Alternate gene names: 93006470
Gene position: 2027215-2029698 (Clockwise)
Preceding gene: 93006468
Following gene: 93006471
Centisome position: 66.25
GC content: 45.21
Gene sequence:
>2484_bases GTGCAAAAATCTAATTCTAAGCAACCACAGTCCTCTAAGCCTGCTATCAATACGCTACCTTCCCAGCAGCGCGGTGTGGT GTTTAGTAGTATTTTATTAATTGTTGTCATCGCTGGCATGGTGCTATTAGCACTATACTTGGTCAAACTTGATCGCACCA TTACTCACAAGTTTGAGGGTAAACGCTGGGATATTCCTGCCAAAGTATATTCGCAGCCATTAGAGCTGTATCAAGGGGCC AACGTCGATAAAGACACGATGAAGAAATGGCTTGAGCTACTTAATTATCAAAGTGATAAAGCCTATGATCGCACGGGTAC TTATCATAAATCGGGTAATACCTATTTTATTCATACACGTGGGTTTACTTATAGCGCTAATGATATAGATCAAGAGCAAG TCATCAAAATGACTATTGCTGGTGATAAGATTGAGTCAGTACAAAGTACTGTGGCGGCGAAAACCGGCATTATCCGTCTT GAACCGGTGAATATCGGTGGTATTTACCCAGATAGTAACGAAGACCGTATGGTTGTCTCGTTAGATCAAGTACCCCAGCC GCTTATTGATGCGCTGATAGCGACAGAAGATCGTGGTTTTTATGAACATAAAGGGGTATCGATACGTGGTATCGCTCGTG CAGTTATCAATAACTTCTCTGGTGGATCACGTCAAGGTGGCTCGACCATCACCCAGCAGCTGATCAAAAACTTTTATCTG AATTCAGACCGCACAATGAAGCGTAAAGCCAATGAAGCCTTGATGGCTGTGCTACTTGAGCTGCATTATAGTAAAGATGA GATTTTGCAAGCATATCTCAACGAGATTTATCTTGGTCAAAATGGCAAACGCTCTATTAATGGTTTTGGGTTGGCATCAC AATTTTATTTTGATAAGCCTCTCAATGAGCTGCGCCTAGATCAGCAAGCATTGCTTGTCGGTATGGCAAAAGGACCGAGC GTTTATAATCCGCGCCGTCATCCAAACGATTCAAAGGCGCGTCGCGATGTTGTGCTTAGCAATATGTTGGCCGTCGGCTC GCTCAGTCAAGAAGACTACGATAAAGCATTAAAAAACTCACTTGGGGTAGTTGATAAGCCTGTTGAAGGTAAAAGCCAAT TCCCTGACTTTTTAGATATCGTCAAGCGTGAACTAAATAAAGTCTATTATTCCGATGATCTCAAAAATGAAGGTCTGATT ATCATCAGTACGATGGACCCTATTGCCCAGTTGGCAGCGGACAAAGCGGTTGAGAGAAAGCTTGGCGAGCTACGCCGTAG TGGTAGCAAAACCAAAGATTTACAAGGGGCACTGGTCAGTGCCAATCCTGAGACGGGTGAGCTGGTTTCTGTTGTCGGTA GCGGCAGCGAGTTTACTGGCTTTAACCGTGCGGTCGATGCCAAACGCCAAGTAGGCTCATTATTAAAGCCGATTATTTAT ATGACTGCTCTCGAAAGTGGTCGCTATAATTTGGCAAGTTCGGTTGATGACTCCCCTATTACGGTCAGTCTAAGCGACGG TACCGAATGGAATCCCAAAAACTATGACAATCGTGATCATGGCTACATACCCTTTACCACAGCGCTGTCGCAATCGTATA ACCATAGTGCCGTACGCTTAGGGATGGAGTTTGGAGTCGACACCTTTGCCAAGCAATTAAAACGTATGGGCGTTAAAGAG AAAATACCTCCTTATCCTGCGGCATTATTGGGCTCAGTCAATCTAAGCCCGATGGACATGCTTGGGGTCTATCAAGTTTT TGCAACGGGTGGTTTCCGTACGCCTATTCATAGTATCCGTAGTGTGATTGATGATCGCGGTAGTATTTTGCAGCGCACAG GCTTAAATACTCAGCGCAGTATTCCGCCTGAGACCAACTTTTTGATTAATTATGCGTTGCAAGATGTCGTCAAGAACGGT ACAGCTAGACGCGTGCAATCGCTTGGTAGCAATCTAAATCTTGCGGGCAAAACAGGTACTACCAATGATTACCGCGATGC GTGGTTTGCCGGTTATAGTGGCAACTATGTCAGCGTGGTTTGGGTTGGACGTGATGATAATAAACCGATTGGGCTTAGTG GCGGTAGCGGTGCTTTGCCAGTTTGGGTTGATTATATGAATCGTCTAAAACTGACACCTGTTGCGCTACCAGAGCCAGAA GGCATCGAATGGCTCTGGTTAGAGAACAACTCAGGCAAGCTGTCTAATGAGCGCTGTGCCAGCGCACGCTACTTGCCTGT TATGTCAGCATATTTGCCCCAAGAAGCCAGCAGCTGTGCGATTGGCTTATACCAACAGGATCGTGCGCGCGAGCAAATGC AGTGGCAAAATCAGCAAGGCGATATCAATAAGCAACGTCGCCGTGAAGGATTAGACATACCAGATGGTGAAGTTATTGAT AATAATAGCAATGATGCCGAGCAAGACGGTGATACACCCAATCGTGTAGATACTTGGTATGATCGTGCTGTTGAATGGTT TTAG
Upstream 100 bases:
>100_bases TGAGAGCAAATGAATGCCTGCGTTGATGATGATAGATTGAAAAATTTGTCAATTTGCGTCATTATGAGGCAGTTTTTCAT CCCAAAATGGTGTGTCATCA
Downstream 100 bases:
>100_bases TGAACTGCTTCTATTTCAGTCGTGATAGCTGAAAATTTGACTTTAAAAGGTTAGTAGCTGAACGATTTTAAGATTTTTAA AACTTAGAAAGGGTTTTAAT
Product: penicillin-binding protein 1B
Products: NA
Alternate protein names: PBP-1b; PBP1b; Murein polymerase; Penicillin-insensitive transglycosylase; Peptidoglycan TGase; Peptidoglycan glycosyltransferase; Penicillin-sensitive transpeptidase; DD-transpeptidase [H]
Number of amino acids: Translated: 827; Mature: 827
Protein sequence:
>827_residues MQKSNSKQPQSSKPAINTLPSQQRGVVFSSILLIVVIAGMVLLALYLVKLDRTITHKFEGKRWDIPAKVYSQPLELYQGA NVDKDTMKKWLELLNYQSDKAYDRTGTYHKSGNTYFIHTRGFTYSANDIDQEQVIKMTIAGDKIESVQSTVAAKTGIIRL EPVNIGGIYPDSNEDRMVVSLDQVPQPLIDALIATEDRGFYEHKGVSIRGIARAVINNFSGGSRQGGSTITQQLIKNFYL NSDRTMKRKANEALMAVLLELHYSKDEILQAYLNEIYLGQNGKRSINGFGLASQFYFDKPLNELRLDQQALLVGMAKGPS VYNPRRHPNDSKARRDVVLSNMLAVGSLSQEDYDKALKNSLGVVDKPVEGKSQFPDFLDIVKRELNKVYYSDDLKNEGLI IISTMDPIAQLAADKAVERKLGELRRSGSKTKDLQGALVSANPETGELVSVVGSGSEFTGFNRAVDAKRQVGSLLKPIIY MTALESGRYNLASSVDDSPITVSLSDGTEWNPKNYDNRDHGYIPFTTALSQSYNHSAVRLGMEFGVDTFAKQLKRMGVKE KIPPYPAALLGSVNLSPMDMLGVYQVFATGGFRTPIHSIRSVIDDRGSILQRTGLNTQRSIPPETNFLINYALQDVVKNG TARRVQSLGSNLNLAGKTGTTNDYRDAWFAGYSGNYVSVVWVGRDDNKPIGLSGGSGALPVWVDYMNRLKLTPVALPEPE GIEWLWLENNSGKLSNERCASARYLPVMSAYLPQEASSCAIGLYQQDRAREQMQWQNQQGDINKQRRREGLDIPDGEVID NNSNDAEQDGDTPNRVDTWYDRAVEWF
Sequences:
>Translated_827_residues MQKSNSKQPQSSKPAINTLPSQQRGVVFSSILLIVVIAGMVLLALYLVKLDRTITHKFEGKRWDIPAKVYSQPLELYQGA NVDKDTMKKWLELLNYQSDKAYDRTGTYHKSGNTYFIHTRGFTYSANDIDQEQVIKMTIAGDKIESVQSTVAAKTGIIRL EPVNIGGIYPDSNEDRMVVSLDQVPQPLIDALIATEDRGFYEHKGVSIRGIARAVINNFSGGSRQGGSTITQQLIKNFYL NSDRTMKRKANEALMAVLLELHYSKDEILQAYLNEIYLGQNGKRSINGFGLASQFYFDKPLNELRLDQQALLVGMAKGPS VYNPRRHPNDSKARRDVVLSNMLAVGSLSQEDYDKALKNSLGVVDKPVEGKSQFPDFLDIVKRELNKVYYSDDLKNEGLI IISTMDPIAQLAADKAVERKLGELRRSGSKTKDLQGALVSANPETGELVSVVGSGSEFTGFNRAVDAKRQVGSLLKPIIY MTALESGRYNLASSVDDSPITVSLSDGTEWNPKNYDNRDHGYIPFTTALSQSYNHSAVRLGMEFGVDTFAKQLKRMGVKE KIPPYPAALLGSVNLSPMDMLGVYQVFATGGFRTPIHSIRSVIDDRGSILQRTGLNTQRSIPPETNFLINYALQDVVKNG TARRVQSLGSNLNLAGKTGTTNDYRDAWFAGYSGNYVSVVWVGRDDNKPIGLSGGSGALPVWVDYMNRLKLTPVALPEPE GIEWLWLENNSGKLSNERCASARYLPVMSAYLPQEASSCAIGLYQQDRAREQMQWQNQQGDINKQRRREGLDIPDGEVID NNSNDAEQDGDTPNRVDTWYDRAVEWF >Mature_827_residues MQKSNSKQPQSSKPAINTLPSQQRGVVFSSILLIVVIAGMVLLALYLVKLDRTITHKFEGKRWDIPAKVYSQPLELYQGA NVDKDTMKKWLELLNYQSDKAYDRTGTYHKSGNTYFIHTRGFTYSANDIDQEQVIKMTIAGDKIESVQSTVAAKTGIIRL EPVNIGGIYPDSNEDRMVVSLDQVPQPLIDALIATEDRGFYEHKGVSIRGIARAVINNFSGGSRQGGSTITQQLIKNFYL NSDRTMKRKANEALMAVLLELHYSKDEILQAYLNEIYLGQNGKRSINGFGLASQFYFDKPLNELRLDQQALLVGMAKGPS VYNPRRHPNDSKARRDVVLSNMLAVGSLSQEDYDKALKNSLGVVDKPVEGKSQFPDFLDIVKRELNKVYYSDDLKNEGLI IISTMDPIAQLAADKAVERKLGELRRSGSKTKDLQGALVSANPETGELVSVVGSGSEFTGFNRAVDAKRQVGSLLKPIIY MTALESGRYNLASSVDDSPITVSLSDGTEWNPKNYDNRDHGYIPFTTALSQSYNHSAVRLGMEFGVDTFAKQLKRMGVKE KIPPYPAALLGSVNLSPMDMLGVYQVFATGGFRTPIHSIRSVIDDRGSILQRTGLNTQRSIPPETNFLINYALQDVVKNG TARRVQSLGSNLNLAGKTGTTNDYRDAWFAGYSGNYVSVVWVGRDDNKPIGLSGGSGALPVWVDYMNRLKLTPVALPEPE GIEWLWLENNSGKLSNERCASARYLPVMSAYLPQEASSCAIGLYQQDRAREQMQWQNQQGDINKQRRREGLDIPDGEVID NNSNDAEQDGDTPNRVDTWYDRAVEWF
Specific function: Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal
COG id: COG0744
COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)
Gene ontology:
Cell location: Cell inner membrane; Single-pass type II membrane protein [H]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: In the C-terminal section; belongs to the transpeptidase family [H]
Homologues:
Organism=Escherichia coli, GI1786343, Length=731, Percent_Identity=38.9876880984952, Blast_Score=478, Evalue=1e-136, Organism=Escherichia coli, GI87082258, Length=243, Percent_Identity=39.917695473251, Blast_Score=172, Evalue=1e-43, Organism=Escherichia coli, GI1788867, Length=563, Percent_Identity=28.7744227353464, Blast_Score=162, Evalue=6e-41, Organism=Escherichia coli, GI1789601, Length=169, Percent_Identity=33.1360946745562, Blast_Score=82, Evalue=1e-16,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR012338 - InterPro: IPR001264 - InterPro: IPR011813 - InterPro: IPR001460 [H]
Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]
EC number: =2.4.1.129 [H]
Molecular weight: Translated: 92002; Mature: 92002
Theoretical pI: Translated: 8.09; Mature: 8.09
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.2 %Cys (Translated Protein) 2.2 %Met (Translated Protein) 2.4 %Cys+Met (Translated Protein) 0.2 %Cys (Mature Protein) 2.2 %Met (Mature Protein) 2.4 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MQKSNSKQPQSSKPAINTLPSQQRGVVFSSILLIVVIAGMVLLALYLVKLDRTITHKFEG CCCCCCCCCCCCCCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC KRWDIPAKVYSQPLELYQGANVDKDTMKKWLELLNYQSDKAYDRTGTYHKSGNTYFIHTR CCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCEECCCCEEEEEEC GFTYSANDIDQEQVIKMTIAGDKIESVQSTVAAKTGIIRLEPVNIGGIYPDSNEDRMVVS CEEECCCCCCHHHEEEEEECCCHHHHHHHHHHHHCCEEEEEEEECCCCCCCCCCCEEEEE LDQVPQPLIDALIATEDRGFYEHKGVSIRGIARAVINNFSGGSRQGGSTITQQLIKNFYL HHHCCHHHHHHHHHCCCCCCHHHCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHC NSDRTMKRKANEALMAVLLELHYSKDEILQAYLNEIYLGQNGKRSINGFGLASQFYFDKP CCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHCCC LNELRLDQQALLVGMAKGPSVYNPRRHPNDSKARRDVVLSNMLAVGSLSQEDYDKALKNS HHHHHCCCHHHHEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHC LGVVDKPVEGKSQFPDFLDIVKRELNKVYYSDDLKNEGLIIISTMDPIAQLAADKAVERK CCCCCCCCCCCCCCCHHHHHHHHHHHHHEECCCCCCCCEEEEECCCHHHHHHHHHHHHHH LGELRRSGSKTKDLQGALVSANPETGELVSVVGSGSEFTGFNRAVDAKRQVGSLLKPIIY HHHHHHCCCCCCCCCCEEEECCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHH MTALESGRYNLASSVDDSPITVSLSDGTEWNPKNYDNRDHGYIPFTTALSQSYNHSAVRL HHHHCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCEEHHHHHHCCCCCHHEEE GMEFGVDTFAKQLKRMGVKEKIPPYPAALLGSVNLSPMDMLGVYQVFATGGFRTPIHSIR HHHHCHHHHHHHHHHCCCHHCCCCCCHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHH SVIDDRGSILQRTGLNTQRSIPPETNFLINYALQDVVKNGTARRVQSLGSNLNLAGKTGT HHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHCCCCCEECCCCCC TNDYRDAWFAGYSGNYVSVVWVGRDDNKPIGLSGGSGALPVWVDYMNRLKLTPVALPEPE CCCCCHHEEECCCCCEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHCCEECEECCCCCC GIEWLWLENNSGKLSNERCASARYLPVMSAYLPQEASSCAIGLYQQDRAREQMQWQNQQG CCEEEEEECCCCCCCCHHHHCCHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHCCCCC DINKQRRREGLDIPDGEVIDNNSNDAEQDGDTPNRVDTWYDRAVEWF CCCHHHHHCCCCCCCCCEECCCCCCCCCCCCCCCHHHHHHHHHHCCC >Mature Secondary Structure MQKSNSKQPQSSKPAINTLPSQQRGVVFSSILLIVVIAGMVLLALYLVKLDRTITHKFEG CCCCCCCCCCCCCCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC KRWDIPAKVYSQPLELYQGANVDKDTMKKWLELLNYQSDKAYDRTGTYHKSGNTYFIHTR CCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCEECCCCEEEEEEC GFTYSANDIDQEQVIKMTIAGDKIESVQSTVAAKTGIIRLEPVNIGGIYPDSNEDRMVVS CEEECCCCCCHHHEEEEEECCCHHHHHHHHHHHHCCEEEEEEEECCCCCCCCCCCEEEEE LDQVPQPLIDALIATEDRGFYEHKGVSIRGIARAVINNFSGGSRQGGSTITQQLIKNFYL HHHCCHHHHHHHHHCCCCCCHHHCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHC NSDRTMKRKANEALMAVLLELHYSKDEILQAYLNEIYLGQNGKRSINGFGLASQFYFDKP CCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHCCC LNELRLDQQALLVGMAKGPSVYNPRRHPNDSKARRDVVLSNMLAVGSLSQEDYDKALKNS HHHHHCCCHHHHEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHC LGVVDKPVEGKSQFPDFLDIVKRELNKVYYSDDLKNEGLIIISTMDPIAQLAADKAVERK CCCCCCCCCCCCCCCHHHHHHHHHHHHHEECCCCCCCCEEEEECCCHHHHHHHHHHHHHH LGELRRSGSKTKDLQGALVSANPETGELVSVVGSGSEFTGFNRAVDAKRQVGSLLKPIIY HHHHHHCCCCCCCCCCEEEECCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHH MTALESGRYNLASSVDDSPITVSLSDGTEWNPKNYDNRDHGYIPFTTALSQSYNHSAVRL HHHHCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCEEHHHHHHCCCCCHHEEE GMEFGVDTFAKQLKRMGVKEKIPPYPAALLGSVNLSPMDMLGVYQVFATGGFRTPIHSIR HHHHCHHHHHHHHHHCCCHHCCCCCCHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHH SVIDDRGSILQRTGLNTQRSIPPETNFLINYALQDVVKNGTARRVQSLGSNLNLAGKTGT HHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHCCCCCEECCCCCC TNDYRDAWFAGYSGNYVSVVWVGRDDNKPIGLSGGSGALPVWVDYMNRLKLTPVALPEPE CCCCCHHEEECCCCCEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHCCEECEECCCCCC GIEWLWLENNSGKLSNERCASARYLPVMSAYLPQEASSCAIGLYQQDRAREQMQWQNQQG CCEEEEEECCCCCCCCHHHHCCHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHCCCCC DINKQRRREGLDIPDGEVIDNNSNDAEQDGDTPNRVDTWYDRAVEWF CCCHHHHHCCCCCCCCCEECCCCCCCCCCCCCCCHHHHHHHHHHCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 6.0
TargetDB status: NA
Availability: NA
References: 10952301 [H]