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The map label for this gene is metG [H]

Identifier: 93006420

GI number: 93006420

Start: 1955163

End: 1957265

Strand: Direct

Name: metG [H]

Synonym: Pcryo_1596

Alternate gene names: 93006420

Gene position: 1955163-1957265 (Clockwise)

Preceding gene: 93006419

Following gene: 93006422

Centisome position: 63.9

GC content: 44.17

Gene sequence:

>2103_bases
ATGAAAGTGCGTGAGATTTTAGTAACCAGTGCCCTGCCCTACGCCAATGGAGACATTCACTTGGGGCATCTTGTAGAGTA
TATCCAAACTGATATTTGGGTACGGTCGATGAAGGCGCAAGGTCATAAGGTGACTTATGTCTGTGCGGATGATGCTCATG
GTACTGCCATCATGCTCAAAGCCGAAGACAATGGCGTAACACCAGAACAGCAAATTGCCAATGTGCAAGCTGCTCATGAA
GCCGATTTTGCTAAGTTTTTGATTAATTTTGACAATTATCATTCAACGCATTCAGAAGAAAATCGGGAATTTTCTGAGCT
AATTTATCGCCGCCTTAGAGATGCAGGTCATATCTCTACTCGTGATGTGGAGCAGCTATTTGACCCTGAAAAACAGCTGT
TTTTAGCCGATCGTTTTGTTAAAGGTACCTGCCCTGAGTGCGCCGCTACCGACCAATATGGTGATAATTGTGAAGTTTGT
GGTACGACTTATGATGCGACAGAACTTAAAAATCCGTACTCTACGTTATCAAATGCGACGCCTATTCTAAAGACTTCAAA
GCATTATTTCTTTGACTTACCAGAGTTTGAGCAATTTTTAAAGGATTGGACGCGCAGTGATAATCGCTTGCAAACCTCAG
TTGCTAATAAGCTGCAAGAGTGGTTCGATGCCGGCTTGACCAGTTGGGATATCTCGCGTGATGCGCCCTACTTTGGCTTT
CAAATTCCAGACACGCCAAGCGATGAGCCAGACAAATACTTCTACGTATGGCTAGATGCGCCTGTTGGCTATATGGCAAG
CTTTCAGAACTTGTGTGATAAACGTGCAGGTACAGAAGAGGCTCTAGACTTTGACCATTATTGGGCGCAAGAAAACGAGC
ATAAAACCGAGGTTTATCACTTCATCGGTAAAGACATCGTTTATTTCCATGCGCTATTTTGGCCTGCCATGCTTGCTGGT
AGTGAACTCCGTACACCAACTGGCGTCTTTGCTCATGGCTTCTTGATGGTCAACGGCGAAAAAATGAGTAAGTCGCGCGG
CACCTTTATTAAAGCCGACACTTATGCTGAGCATTTGCATCCTGAGTACTTGCGTTATTATTTTGCGAGTAAGTTATCTG
ACAAAGTCGAGGACATTAACCTTGATTTAGAAGACTTTATGCAAAAGGTTAACTCTGATCTCGTTGGTAAAGTAGTCAAT
ATCGCCAGTCGCAGCGCTGGATTTTTGGTCAAAAAGTATGATGGTATGCTGACAGACGTCTGTGCTGAGCCAAGCCTATT
AGAAGACATCACCAAGACGGGCGATGAAATTGCTGCGGCTTATGAAAACCGTGAATTTTCTCGTGCCATGCGCCTGATTA
TGCAGTGTGCTGATAAAGCCAATGAATACATCGATGACAAAAAACCGTGGGCGCTTGCCAAGGTTGAAGGCGCAGAGCAA
GAAGTTCAAGACGTCTGCTCAGTAGCAATCAATATCTTCCGTCAATTAATGGTATATCTTGCGCCTGTATTACCTGAGCT
GACTGCCAATGCTAAAGAATTTTTGAACATTGATGATTTAAGTTTTGCCAGCCGTAATGAGTGGTTGCTTGGTCATCAAA
TTAATAAGTTTAAACCACTGATGCAGCGTATTGAAGAAAAAGATATTGCAGCGATGGTTGAAGATTCTAAAGCCTCATTG
ACACAAGTAGGTGCGCCTACCGCTAGCCAAGACGATAAAGCAGCAGCAAAAAATACCTCGCCTGCAGCAATGCCAAGCAG
TACCGAACAAGCAGACTATATCGGTATCGAGGACTTTGCCAAAGTCGAGATGAAAGTCGCTCATGTTATCGCTTGCAACC
ATGTCGAAGGCGCGGACAAACTGCTTCAGTTCACACTAGATGTTGGTGAAGCACAACCTCGCAACGTCTTTAGTGGTATT
CGCAAATTCTATGAGCCTGAGCAATTACAAGGTAAAAAGGTTATTTGCGTGACCAATTTGGCACCGCGTAAGATGAAATT
TGGCATATCAGAAGGCATGGTATTGTCGTCAGGCGACCCGAAAACAGGTCTGGTGGTAATTACCTTACCTGATGAAGCCG
TTATCGGTGATTCACTGGCTTAA

Upstream 100 bases:

>100_bases
ATTCGTAAGGAATATCACAAATTTAGCCGAATTACGGTATCATAGGCACACTTTTTCAATTTCTATTGATAACCAACCCG
TTTATATAAGAATATAGGTG

Downstream 100 bases:

>100_bases
AAACGTTAAATTAATCAAGTAATAATAAAAGAACAAAACAAAAAACGGTCTAAATAGACCGTTTTTTATGTTAAAAAGAA
TAGAAAGTAAGCTTGCTGTT

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 700; Mature: 700

Protein sequence:

>700_residues
MKVREILVTSALPYANGDIHLGHLVEYIQTDIWVRSMKAQGHKVTYVCADDAHGTAIMLKAEDNGVTPEQQIANVQAAHE
ADFAKFLINFDNYHSTHSEENREFSELIYRRLRDAGHISTRDVEQLFDPEKQLFLADRFVKGTCPECAATDQYGDNCEVC
GTTYDATELKNPYSTLSNATPILKTSKHYFFDLPEFEQFLKDWTRSDNRLQTSVANKLQEWFDAGLTSWDISRDAPYFGF
QIPDTPSDEPDKYFYVWLDAPVGYMASFQNLCDKRAGTEEALDFDHYWAQENEHKTEVYHFIGKDIVYFHALFWPAMLAG
SELRTPTGVFAHGFLMVNGEKMSKSRGTFIKADTYAEHLHPEYLRYYFASKLSDKVEDINLDLEDFMQKVNSDLVGKVVN
IASRSAGFLVKKYDGMLTDVCAEPSLLEDITKTGDEIAAAYENREFSRAMRLIMQCADKANEYIDDKKPWALAKVEGAEQ
EVQDVCSVAINIFRQLMVYLAPVLPELTANAKEFLNIDDLSFASRNEWLLGHQINKFKPLMQRIEEKDIAAMVEDSKASL
TQVGAPTASQDDKAAAKNTSPAAMPSSTEQADYIGIEDFAKVEMKVAHVIACNHVEGADKLLQFTLDVGEAQPRNVFSGI
RKFYEPEQLQGKKVICVTNLAPRKMKFGISEGMVLSSGDPKTGLVVITLPDEAVIGDSLA

Sequences:

>Translated_700_residues
MKVREILVTSALPYANGDIHLGHLVEYIQTDIWVRSMKAQGHKVTYVCADDAHGTAIMLKAEDNGVTPEQQIANVQAAHE
ADFAKFLINFDNYHSTHSEENREFSELIYRRLRDAGHISTRDVEQLFDPEKQLFLADRFVKGTCPECAATDQYGDNCEVC
GTTYDATELKNPYSTLSNATPILKTSKHYFFDLPEFEQFLKDWTRSDNRLQTSVANKLQEWFDAGLTSWDISRDAPYFGF
QIPDTPSDEPDKYFYVWLDAPVGYMASFQNLCDKRAGTEEALDFDHYWAQENEHKTEVYHFIGKDIVYFHALFWPAMLAG
SELRTPTGVFAHGFLMVNGEKMSKSRGTFIKADTYAEHLHPEYLRYYFASKLSDKVEDINLDLEDFMQKVNSDLVGKVVN
IASRSAGFLVKKYDGMLTDVCAEPSLLEDITKTGDEIAAAYENREFSRAMRLIMQCADKANEYIDDKKPWALAKVEGAEQ
EVQDVCSVAINIFRQLMVYLAPVLPELTANAKEFLNIDDLSFASRNEWLLGHQINKFKPLMQRIEEKDIAAMVEDSKASL
TQVGAPTASQDDKAAAKNTSPAAMPSSTEQADYIGIEDFAKVEMKVAHVIACNHVEGADKLLQFTLDVGEAQPRNVFSGI
RKFYEPEQLQGKKVICVTNLAPRKMKFGISEGMVLSSGDPKTGLVVITLPDEAVIGDSLA
>Mature_700_residues
MKVREILVTSALPYANGDIHLGHLVEYIQTDIWVRSMKAQGHKVTYVCADDAHGTAIMLKAEDNGVTPEQQIANVQAAHE
ADFAKFLINFDNYHSTHSEENREFSELIYRRLRDAGHISTRDVEQLFDPEKQLFLADRFVKGTCPECAATDQYGDNCEVC
GTTYDATELKNPYSTLSNATPILKTSKHYFFDLPEFEQFLKDWTRSDNRLQTSVANKLQEWFDAGLTSWDISRDAPYFGF
QIPDTPSDEPDKYFYVWLDAPVGYMASFQNLCDKRAGTEEALDFDHYWAQENEHKTEVYHFIGKDIVYFHALFWPAMLAG
SELRTPTGVFAHGFLMVNGEKMSKSRGTFIKADTYAEHLHPEYLRYYFASKLSDKVEDINLDLEDFMQKVNSDLVGKVVN
IASRSAGFLVKKYDGMLTDVCAEPSLLEDITKTGDEIAAAYENREFSRAMRLIMQCADKANEYIDDKKPWALAKVEGAEQ
EVQDVCSVAINIFRQLMVYLAPVLPELTANAKEFLNIDDLSFASRNEWLLGHQINKFKPLMQRIEEKDIAAMVEDSKASL
TQVGAPTASQDDKAAAKNTSPAAMPSSTEQADYIGIEDFAKVEMKVAHVIACNHVEGADKLLQFTLDVGEAQPRNVFSGI
RKFYEPEQLQGKKVICVTNLAPRKMKFGISEGMVLSSGDPKTGLVVITLPDEAVIGDSLA

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI14043022, Length=609, Percent_Identity=27.7504105090312, Blast_Score=247, Evalue=3e-65,
Organism=Homo sapiens, GI24308436, Length=527, Percent_Identity=23.9089184060721, Blast_Score=128, Evalue=2e-29,
Organism=Escherichia coli, GI1788432, Length=689, Percent_Identity=54.5718432510885, Blast_Score=789, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17540794, Length=637, Percent_Identity=27.4725274725275, Blast_Score=258, Evalue=1e-68,
Organism=Caenorhabditis elegans, GI212645096, Length=525, Percent_Identity=24.1904761904762, Blast_Score=134, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI71992781, Length=350, Percent_Identity=26, Blast_Score=109, Evalue=5e-24,
Organism=Saccharomyces cerevisiae, GI6321704, Length=560, Percent_Identity=29.1071428571429, Blast_Score=241, Evalue=3e-64,
Organism=Saccharomyces cerevisiae, GI6321610, Length=552, Percent_Identity=22.2826086956522, Blast_Score=109, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24655311, Length=562, Percent_Identity=29.1814946619217, Blast_Score=233, Evalue=5e-61,
Organism=Drosophila melanogaster, GI21358483, Length=529, Percent_Identity=22.4952741020794, Blast_Score=124, Evalue=2e-28,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080
- InterPro:   IPR013155 [H]

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g; PF01588 tRNA_bind [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 78779; Mature: 78779

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS50886 TRBD ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVREILVTSALPYANGDIHLGHLVEYIQTDIWVRSMKAQGHKVTYVCADDAHGTAIMLK
CCHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCEEEEE
AEDNGVTPEQQIANVQAAHEADFAKFLINFDNYHSTHSEENREFSELIYRRLRDAGHIST
ECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCH
RDVEQLFDPEKQLFLADRFVKGTCPECAATDQYGDNCEVCGTTYDATELKNPYSTLSNAT
HHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCHHHHHCCCC
PILKTSKHYFFDLPEFEQFLKDWTRSDNRLQTSVANKLQEWFDAGLTSWDISRDAPYFGF
CCEECCCCCEECCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCE
QIPDTPSDEPDKYFYVWLDAPVGYMASFQNLCDKRAGTEEALDFDHYWAQENEHKTEVYH
ECCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCHHHCCCHHHHCCCCCCHHHHHH
FIGKDIVYFHALFWPAMLAGSELRTPTGVFAHGFLMVNGEKMSKSRGTFIKADTYAEHLH
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEECCHHHCCCCCEEEECHHHHHCC
PEYLRYYFASKLSDKVEDINLDLEDFMQKVNSDLVGKVVNIASRSAGFLVKKYDGMLTDV
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCHHHHHHHHHHHCCCCCCEEEHHCCHHHHH
CAEPSLLEDITKTGDEIAAAYENREFSRAMRLIMQCADKANEYIDDKKPWALAKVEGAEQ
HCCCHHHHHHHHCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHH
EVQDVCSVAINIFRQLMVYLAPVLPELTANAKEFLNIDDLSFASRNEWLLGHQINKFKPL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCEEECCCHHHHHHH
MQRIEEKDIAAMVEDSKASLTQVGAPTASQDDKAAAKNTSPAAMPSSTEQADYIGIEDFA
HHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCHHHHH
KVEMKVAHVIACNHVEGADKLLQFTLDVGEAQPRNVFSGIRKFYEPEQLQGKKVICVTNL
HHHHHHHHHEEECCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHCCCEEEEEECC
APRKMKFGISEGMVLSSGDPKTGLVVITLPDEAVIGDSLA
CCCHHHCCCCCCEEEECCCCCCCEEEEECCCCCCCCCCCC
>Mature Secondary Structure
MKVREILVTSALPYANGDIHLGHLVEYIQTDIWVRSMKAQGHKVTYVCADDAHGTAIMLK
CCHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCEEEEE
AEDNGVTPEQQIANVQAAHEADFAKFLINFDNYHSTHSEENREFSELIYRRLRDAGHIST
ECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCH
RDVEQLFDPEKQLFLADRFVKGTCPECAATDQYGDNCEVCGTTYDATELKNPYSTLSNAT
HHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCHHHHHCCCC
PILKTSKHYFFDLPEFEQFLKDWTRSDNRLQTSVANKLQEWFDAGLTSWDISRDAPYFGF
CCEECCCCCEECCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCE
QIPDTPSDEPDKYFYVWLDAPVGYMASFQNLCDKRAGTEEALDFDHYWAQENEHKTEVYH
ECCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCHHHCCCHHHHCCCCCCHHHHHH
FIGKDIVYFHALFWPAMLAGSELRTPTGVFAHGFLMVNGEKMSKSRGTFIKADTYAEHLH
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEECCHHHCCCCCEEEECHHHHHCC
PEYLRYYFASKLSDKVEDINLDLEDFMQKVNSDLVGKVVNIASRSAGFLVKKYDGMLTDV
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCHHHHHHHHHHHCCCCCCEEEHHCCHHHHH
CAEPSLLEDITKTGDEIAAAYENREFSRAMRLIMQCADKANEYIDDKKPWALAKVEGAEQ
HCCCHHHHHHHHCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHH
EVQDVCSVAINIFRQLMVYLAPVLPELTANAKEFLNIDDLSFASRNEWLLGHQINKFKPL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCEEECCCHHHHHHH
MQRIEEKDIAAMVEDSKASLTQVGAPTASQDDKAAAKNTSPAAMPSSTEQADYIGIEDFA
HHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCHHHHH
KVEMKVAHVIACNHVEGADKLLQFTLDVGEAQPRNVFSGIRKFYEPEQLQGKKVICVTNL
HHHHHHHHHEEECCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHCCCEEEEEECC
APRKMKFGISEGMVLSSGDPKTGLVVITLPDEAVIGDSLA
CCCHHHCCCCCCEEEECCCCCCCEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA