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The map label for this gene is ttcA [H]

Identifier: 93005608

GI number: 93005608

Start: 916602

End: 917747

Strand: Direct

Name: ttcA [H]

Synonym: Pcryo_0778

Alternate gene names: 93005608

Gene position: 916602-917747 (Clockwise)

Preceding gene: 93005595

Following gene: 93005615

Centisome position: 29.96

GC content: 43.98

Gene sequence:

>1146_bases
ATGACTGCAGCAACCTTATTTACACCATCAATCACGCCTCAGTTTGACAAGGCTTCTATCGATATTGACGTGAATCATCA
TGATAAAACAACTGATATCGAACAGATGTTATATGATGATGTCGATGACAGAAATGATGATTTTATGGATGAGCAAGCAG
ATAACATATTGGCTGATACTCAATCACTGACCGAGTCGGTTCACTTTCGGAAATTGCAAAAGAAGCTTCGTCGACAAGTA
TCATGGGCGATTCGCGACTTTAATATGATTGAAGATGGCGATGTGGTGATGGTCTGCGTATCAGGCGGTAAAGACAGCTA
TACGTTGCTCGATATCTTGCTGCTATTAAAACGTATCGCCCCGATTCATTTTGATATCGTTGCCGTCAATCTTGACCAAA
AGCAGCCCGGCTATCCAGAAGAGGTGTTGCCCGCCTATCTAAACGAGCAGGGCATCGCTCATTATATTCTGGAAAAAGAC
ACTTATAGTATTGTCAAAAGCGTGGTGCCAGAAGGGAAAACCTATTGCTCGGCGTGTTCACGTCTGCGTCGAGGCTCACT
CTATGGTTTCGCCAAACAAATCGGTGCGACCAAGATTGCGCTTGGGCACCATCGCGATGATATGTTGGCGACTTTCTTTT
TAAACTTATTTCATGGTGGCGCGTTGAAATCGATGCCGCCCAAATTGCTCAGTGATGACAAGCAAAACATGCTCATCCGT
CCACTGGCTTATGTGGAAGAAAAAGACATCATTGAATATTCGAACCTAAAAGAATTCCCGATTATTCCGTGTAATCTCTG
TGGCAGCCAGACCAATCTGCAACGGGCCATTATTAATGACATGCTACGAGAATGGGACGATGCTCATCCTCAGCGCCTAG
CGTCTATTTTTAAAGCGATGCAAAATGTTGCGCCCTCGCAATTGGCAGATAGAGAGCTGTTTGATTTTGAGACATTAAGT
CTGGATCGTGACGATAACGAGCGTTTATTTGAAGGCGATAATATTCAAGCGGGGCAAATAGAGAGTCTGGCTGAGATTGG
TTTGCCAGTATCACCAGAAACGCAGATATTCAATCCAGACTTTGCTAATGCAGAAAAGGGCAGCCGTGCACCGAAAAAAA
TTCCAACGATTAATCCAGTGATTTAA

Upstream 100 bases:

>100_bases
GTTAGCCATACGCAGATAGCTTTTATTGCAGCCTTTTGAATAAATCGATTGATTATCTCTTTTGAAGTAACGCCATTCTT
GTCAAGATATTGAGTAACTT

Downstream 100 bases:

>100_bases
TGACTTGATGCTGTCTACGTTCTTCAAATAAAAAAACCAGCTCATGATAGATTCATGGCTGGTTTTTTTATTTTAATCAT
ATTTTAAACAGTTTAGCTTT

Product: C32 tRNA thiolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 381; Mature: 380

Protein sequence:

>381_residues
MTAATLFTPSITPQFDKASIDIDVNHHDKTTDIEQMLYDDVDDRNDDFMDEQADNILADTQSLTESVHFRKLQKKLRRQV
SWAIRDFNMIEDGDVVMVCVSGGKDSYTLLDILLLLKRIAPIHFDIVAVNLDQKQPGYPEEVLPAYLNEQGIAHYILEKD
TYSIVKSVVPEGKTYCSACSRLRRGSLYGFAKQIGATKIALGHHRDDMLATFFLNLFHGGALKSMPPKLLSDDKQNMLIR
PLAYVEEKDIIEYSNLKEFPIIPCNLCGSQTNLQRAIINDMLREWDDAHPQRLASIFKAMQNVAPSQLADRELFDFETLS
LDRDDNERLFEGDNIQAGQIESLAEIGLPVSPETQIFNPDFANAEKGSRAPKKIPTINPVI

Sequences:

>Translated_381_residues
MTAATLFTPSITPQFDKASIDIDVNHHDKTTDIEQMLYDDVDDRNDDFMDEQADNILADTQSLTESVHFRKLQKKLRRQV
SWAIRDFNMIEDGDVVMVCVSGGKDSYTLLDILLLLKRIAPIHFDIVAVNLDQKQPGYPEEVLPAYLNEQGIAHYILEKD
TYSIVKSVVPEGKTYCSACSRLRRGSLYGFAKQIGATKIALGHHRDDMLATFFLNLFHGGALKSMPPKLLSDDKQNMLIR
PLAYVEEKDIIEYSNLKEFPIIPCNLCGSQTNLQRAIINDMLREWDDAHPQRLASIFKAMQNVAPSQLADRELFDFETLS
LDRDDNERLFEGDNIQAGQIESLAEIGLPVSPETQIFNPDFANAEKGSRAPKKIPTINPVI
>Mature_380_residues
TAATLFTPSITPQFDKASIDIDVNHHDKTTDIEQMLYDDVDDRNDDFMDEQADNILADTQSLTESVHFRKLQKKLRRQVS
WAIRDFNMIEDGDVVMVCVSGGKDSYTLLDILLLLKRIAPIHFDIVAVNLDQKQPGYPEEVLPAYLNEQGIAHYILEKDT
YSIVKSVVPEGKTYCSACSRLRRGSLYGFAKQIGATKIALGHHRDDMLATFFLNLFHGGALKSMPPKLLSDDKQNMLIRP
LAYVEEKDIIEYSNLKEFPIIPCNLCGSQTNLQRAIINDMLREWDDAHPQRLASIFKAMQNVAPSQLADRELFDFETLSL
DRDDNERLFEGDNIQAGQIESLAEIGLPVSPETQIFNPDFANAEKGSRAPKKIPTINPVI

Specific function: Required for the thiolation of cytidine in position 32 of tRNA, to form 2-thiocytidine (s(2)C32) [H]

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TtcA family [H]

Homologues:

Organism=Homo sapiens, GI222537719, Length=232, Percent_Identity=25.4310344827586, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1787606, Length=266, Percent_Identity=61.2781954887218, Blast_Score=353, Evalue=1e-98,
Organism=Caenorhabditis elegans, GI193211090, Length=292, Percent_Identity=27.7397260273973, Blast_Score=115, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI17540090, Length=236, Percent_Identity=26.271186440678, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24651972, Length=229, Percent_Identity=24.0174672489083, Blast_Score=65, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012089
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3 [H]

EC number: NA

Molecular weight: Translated: 43047; Mature: 42916

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAATLFTPSITPQFDKASIDIDVNHHDKTTDIEQMLYDDVDDRNDDFMDEQADNILADT
CCCCEEECCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
QSLTESVHFRKLQKKLRRQVSWAIRDFNMIEDGDVVMVCVSGGKDSYTLLDILLLLKRIA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCEEEEEECCCCCCHHHHHHHHHHHHHC
PIHFDIVAVNLDQKQPGYPEEVLPAYLNEQGIAHYILEKDTYSIVKSVVPEGKTYCSACS
CCEEEEEEEEECCCCCCCCHHHHHHHHCCCCCEEHHHHHHHHHHHHHHCCCCHHHHHHHH
RLRRGSLYGFAKQIGATKIALGHHRDDMLATFFLNLFHGGALKSMPPKLLSDDKQNMLIR
HHHCCCHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCCCEEH
PLAYVEEKDIIEYSNLKEFPIIPCNLCGSQTNLQRAIINDMLREWDDAHPQRLASIFKAM
HHHHHCHHCCHHHCCCCCCCCEEHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
QNVAPSQLADRELFDFETLSLDRDDNERLFEGDNIQAGQIESLAEIGLPVSPETQIFNPD
HCCCHHHHHCCHHHCCCEEECCCCCCCCEECCCCCCCCHHHHHHHCCCCCCCCCCCCCCC
FANAEKGSRAPKKIPTINPVI
CCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TAATLFTPSITPQFDKASIDIDVNHHDKTTDIEQMLYDDVDDRNDDFMDEQADNILADT
CCCEEECCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
QSLTESVHFRKLQKKLRRQVSWAIRDFNMIEDGDVVMVCVSGGKDSYTLLDILLLLKRIA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCEEEEEECCCCCCHHHHHHHHHHHHHC
PIHFDIVAVNLDQKQPGYPEEVLPAYLNEQGIAHYILEKDTYSIVKSVVPEGKTYCSACS
CCEEEEEEEEECCCCCCCCHHHHHHHHCCCCCEEHHHHHHHHHHHHHHCCCCHHHHHHHH
RLRRGSLYGFAKQIGATKIALGHHRDDMLATFFLNLFHGGALKSMPPKLLSDDKQNMLIR
HHHCCCHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCCCEEH
PLAYVEEKDIIEYSNLKEFPIIPCNLCGSQTNLQRAIINDMLREWDDAHPQRLASIFKAM
HHHHHCHHCCHHHCCCCCCCCEEHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
QNVAPSQLADRELFDFETLSLDRDDNERLFEGDNIQAGQIESLAEIGLPVSPETQIFNPD
HCCCHHHHHCCHHHCCCEEECCCCCCCCEECCCCCCCCHHHHHHHCCCCCCCCCCCCCCC
FANAEKGSRAPKKIPTINPVI
CCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA