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The map label for this gene is pgk

Identifier: 93005511

GI number: 93005511

Start: 807450

End: 808652

Strand: Direct

Name: pgk

Synonym: Pcryo_0681

Alternate gene names: 93005511

Gene position: 807450-808652 (Clockwise)

Preceding gene: 93005509

Following gene: 93005512

Centisome position: 26.39

GC content: 45.89

Gene sequence:

>1203_bases
ATGAATTTTTTGCGTATGAGCGAGCTGAGTTTGACCGATAAAGTAGTGTTGATTCGTGAAGACCTTAATGTCCCTATTAA
AGCGGGTAAAGTGGCTAGTGATGCACGCCTGCAAGCAAGTTTGCCGACGATTAAGATGGCGCTAGAAAAGGGTGCAGCAG
TGATAGTCTGCTCGCATTTAGGACGCCCGACAGAAGGCAATCCTGAAGCCATTTATTCGTTAGCACCCGTTGCTGACTAT
TTAAGCGATAAACTATCCGCGCCTGTTACGCTGAATAGTGACTACTTCACTCAAGGCGTCGCTATCGAAGCAGGTGAAGT
CGTACTGCTAGAGAACGTACGTTTTAATGAAGGCGAAAAGAAGAATGATGCAGCGCTTGCACAAAAATATGCTGATTTGT
GCGATGTATTTGTGATGGACGCTTTTGGCACAGCACATCGCGCCCAAGCATCAACAGAAGGCGTAACCCAAGCGATGCAT
AAAGCAGGTAAGACCGCCTGTGCTGGACCTTTATTGGCAGCAGAGCTTGATGCTTTAAGCCTAGCACTTGAAACGCCAGC
ACAACCTATGCTCGCTATCGTTGGTGGCTCAAAAGTTTCTACCAAATTAGAGGTTTTACATAGTTTGGCTGAGCTTTGCT
CACAGATTATCGTTGGTGGCGGGATTGCAAATACCTTCTTAGCCGCGCAAGGTCATAGTGTTGGTGCGTCACTTTATGAA
GCAGATCTGCTCGATACAGCTCGTGAAATCATGGGTAAAACTGAGATTTTATTGCCTGAATATGTCGTCGTTGCTGATAA
AAGCGACATTGATTTTGCTGACTTCACTGGCTCATTACAACAAGCCTCTGCGACCATCAAATCTGTCGACACGATTGGCG
AAAATGACATGATATTAGATATCGCGCCAGATAGTGCTATAAAATTGGCAGAGGCAATTACCAAGGCGAAAACTATCTTA
TGGAATGGTCCAGTCGGCGTTTTTGAAGTGGATGCGTTTGGTCAGGGTACTCAGATAGTGGCAGAAGCGGTCAAAAATAG
CCAAGGTTTCTCGATTGCAGGTGGCGGTGATACACTTGCTGCGATTGATAAATATCAAGTAGCGGATGGCGTCAGCTATA
TGTCAACGGGTGGCGGTGCGTTCTTAGAGTTTGTAGAAGGCAAAGTCTTGCCAGCGGTTGCTGCTTTAGAGATTAATGCA
TAA

Upstream 100 bases:

>100_bases
AGCAATAGGATTTGATGAAATAAATTTGCTAAAGAGTATCCAATAAACCGTTGGCGAACCGTCATACAGATTGATAACAG
ATGATATATAGGAGCAGGTA

Downstream 100 bases:

>100_bases
CCTCTTAACGATACTTCAGCAGATGAGTAACATTAGCCGTACAATTTAGCCGCAGTATCTAAGGTACGCTGTACGGATAA
TATTTTGGCTACTATTCATA

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 400; Mature: 400

Protein sequence:

>400_residues
MNFLRMSELSLTDKVVLIREDLNVPIKAGKVASDARLQASLPTIKMALEKGAAVIVCSHLGRPTEGNPEAIYSLAPVADY
LSDKLSAPVTLNSDYFTQGVAIEAGEVVLLENVRFNEGEKKNDAALAQKYADLCDVFVMDAFGTAHRAQASTEGVTQAMH
KAGKTACAGPLLAAELDALSLALETPAQPMLAIVGGSKVSTKLEVLHSLAELCSQIIVGGGIANTFLAAQGHSVGASLYE
ADLLDTAREIMGKTEILLPEYVVVADKSDIDFADFTGSLQQASATIKSVDTIGENDMILDIAPDSAIKLAEAITKAKTIL
WNGPVGVFEVDAFGQGTQIVAEAVKNSQGFSIAGGGDTLAAIDKYQVADGVSYMSTGGGAFLEFVEGKVLPAVAALEINA

Sequences:

>Translated_400_residues
MNFLRMSELSLTDKVVLIREDLNVPIKAGKVASDARLQASLPTIKMALEKGAAVIVCSHLGRPTEGNPEAIYSLAPVADY
LSDKLSAPVTLNSDYFTQGVAIEAGEVVLLENVRFNEGEKKNDAALAQKYADLCDVFVMDAFGTAHRAQASTEGVTQAMH
KAGKTACAGPLLAAELDALSLALETPAQPMLAIVGGSKVSTKLEVLHSLAELCSQIIVGGGIANTFLAAQGHSVGASLYE
ADLLDTAREIMGKTEILLPEYVVVADKSDIDFADFTGSLQQASATIKSVDTIGENDMILDIAPDSAIKLAEAITKAKTIL
WNGPVGVFEVDAFGQGTQIVAEAVKNSQGFSIAGGGDTLAAIDKYQVADGVSYMSTGGGAFLEFVEGKVLPAVAALEINA
>Mature_400_residues
MNFLRMSELSLTDKVVLIREDLNVPIKAGKVASDARLQASLPTIKMALEKGAAVIVCSHLGRPTEGNPEAIYSLAPVADY
LSDKLSAPVTLNSDYFTQGVAIEAGEVVLLENVRFNEGEKKNDAALAQKYADLCDVFVMDAFGTAHRAQASTEGVTQAMH
KAGKTACAGPLLAAELDALSLALETPAQPMLAIVGGSKVSTKLEVLHSLAELCSQIIVGGGIANTFLAAQGHSVGASLYE
ADLLDTAREIMGKTEILLPEYVVVADKSDIDFADFTGSLQQASATIKSVDTIGENDMILDIAPDSAIKLAEAITKAKTIL
WNGPVGVFEVDAFGQGTQIVAEAVKNSQGFSIAGGGDTLAAIDKYQVADGVSYMSTGGGAFLEFVEGKVLPAVAALEINA

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family

Homologues:

Organism=Homo sapiens, GI4505763, Length=428, Percent_Identity=40.4205607476636, Blast_Score=253, Evalue=3e-67,
Organism=Homo sapiens, GI31543397, Length=426, Percent_Identity=39.2018779342723, Blast_Score=246, Evalue=3e-65,
Organism=Escherichia coli, GI1789294, Length=400, Percent_Identity=61.75, Blast_Score=471, Evalue=1e-134,
Organism=Caenorhabditis elegans, GI17508823, Length=428, Percent_Identity=40.8878504672897, Blast_Score=268, Evalue=5e-72,
Organism=Saccharomyces cerevisiae, GI6319857, Length=422, Percent_Identity=38.6255924170616, Blast_Score=235, Evalue=9e-63,
Organism=Drosophila melanogaster, GI17136394, Length=431, Percent_Identity=39.4431554524362, Blast_Score=236, Evalue=1e-62,
Organism=Drosophila melanogaster, GI24581203, Length=423, Percent_Identity=37.5886524822695, Blast_Score=224, Evalue=1e-58,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): PGK_PSYCK (Q1QCY9)

Other databases:

- EMBL:   CP000323
- RefSeq:   YP_579948.1
- ProteinModelPortal:   Q1QCY9
- SMR:   Q1QCY9
- STRING:   Q1QCY9
- GeneID:   4034374
- GenomeReviews:   CP000323_GR
- KEGG:   pcr:Pcryo_0681
- NMPDR:   fig|335284.3.peg.44
- eggNOG:   COG0126
- HOGENOM:   HBG453500
- OMA:   DCIGDEV
- PhylomeDB:   Q1QCY9
- ProtClustDB:   PRK00073
- BioCyc:   PCRY335284:PCRYO_0681-MONOMER
- GO:   GO:0005737
- GO:   GO:0006096
- HAMAP:   MF_00145
- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824
- Gene3D:   G3DSA:3.40.50.1270
- Gene3D:   G3DSA:3.40.50.1260
- PANTHER:   PTHR11406
- PIRSF:   PIRSF000724
- PRINTS:   PR00477

Pfam domain/function: PF00162 PGK; SSF53748 PGK

EC number: =2.7.2.3

Molecular weight: Translated: 41736; Mature: 41736

Theoretical pI: Translated: 4.31; Mature: 4.31

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: BINDING 36-36 BINDING 114-114 BINDING 147-147 BINDING 202-202 BINDING 329-329

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFLRMSELSLTDKVVLIREDLNVPIKAGKVASDARLQASLPTIKMALEKGAAVIVCSHL
CCCCCCCCCCCCCEEEEEEECCCCCEECCCCCCCCEEECCCCHHHHHHHCCCEEEEEECC
GRPTEGNPEAIYSLAPVADYLSDKLSAPVTLNSDYFTQGVAIEAGEVVLLENVRFNEGEK
CCCCCCCHHHHEEHHHHHHHHHHHCCCCEEECCCCCCCCEEEECCCEEEEECCCCCCCCC
KNDAALAQKYADLCDVFVMDAFGTAHRAQASTEGVTQAMHKAGKTACAGPLLAAELDALS
CCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHCCCHHHHHHHHHH
LALETPAQPMLAIVGGSKVSTKLEVLHSLAELCSQIIVGGGIANTFLAAQGHSVGASLYE
HHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHEECCCCCCHHHHH
ADLLDTAREIMGKTEILLPEYVVVADKSDIDFADFTGSLQQASATIKSVDTIGENDMILD
HHHHHHHHHHHCCCEEECCCEEEEECCCCCCHHHHCCCHHHHHHHHHHHHHCCCCCEEEE
IAPDSAIKLAEAITKAKTILWNGPVGVFEVDAFGQGTQIVAEAVKNSQGFSIAGGGDTLA
ECCCHHHHHHHHHHHHHEEEECCCCCEEEECCCCCCHHHHHHHHHCCCCEEEECCCCCCH
AIDKYQVADGVSYMSTGGGAFLEFVEGKVLPAVAALEINA
HHHHHHHHCCCHHHCCCCCHHHHHHCCCCCCEEEEEEECC
>Mature Secondary Structure
MNFLRMSELSLTDKVVLIREDLNVPIKAGKVASDARLQASLPTIKMALEKGAAVIVCSHL
CCCCCCCCCCCCCEEEEEEECCCCCEECCCCCCCCEEECCCCHHHHHHHCCCEEEEEECC
GRPTEGNPEAIYSLAPVADYLSDKLSAPVTLNSDYFTQGVAIEAGEVVLLENVRFNEGEK
CCCCCCCHHHHEEHHHHHHHHHHHCCCCEEECCCCCCCCEEEECCCEEEEECCCCCCCCC
KNDAALAQKYADLCDVFVMDAFGTAHRAQASTEGVTQAMHKAGKTACAGPLLAAELDALS
CCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHCCCHHHHHHHHHH
LALETPAQPMLAIVGGSKVSTKLEVLHSLAELCSQIIVGGGIANTFLAAQGHSVGASLYE
HHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHEECCCCCCHHHHH
ADLLDTAREIMGKTEILLPEYVVVADKSDIDFADFTGSLQQASATIKSVDTIGENDMILD
HHHHHHHHHHHCCCEEECCCEEEEECCCCCCHHHHCCCHHHHHHHHHHHHHCCCCCEEEE
IAPDSAIKLAEAITKAKTILWNGPVGVFEVDAFGQGTQIVAEAVKNSQGFSIAGGGDTLA
ECCCHHHHHHHHHHHHHEEEECCCCCEEEECCCCCCHHHHHHHHHCCCCEEEECCCCCCH
AIDKYQVADGVSYMSTGGGAFLEFVEGKVLPAVAALEINA
HHHHHHHHCCCHHHCCCCCHHHHHHCCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA