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The map label for this gene is thiE [H]

Identifier: 93005509

GI number: 93005509

Start: 806090

End: 806770

Strand: Direct

Name: thiE [H]

Synonym: Pcryo_0679

Alternate gene names: 93005509

Gene position: 806090-806770 (Clockwise)

Preceding gene: 93005508

Following gene: 93005511

Centisome position: 26.34

GC content: 42.73

Gene sequence:

>681_bases
ATGATTCAGTCATCAGTCTCTACGGTTTCTCATACTATACCTAAGCTATATTTGCTGACTAATGATGATGAGTTTAGCCT
TTTATATCAAAAGTTAGAAGCCGCTTTAGCAACAGGATTGATTGCTCTTTTACAAATACGTCGTAAGCAGATATTAGCAT
TGCCTGACGGCGAATCTGTTCTTTATAAAGAGGCGCTACAAATCGTTAATTTGGCTAAAACCTACAATGTGCCAGTATTG
ATAAATGACAATATTGCTTTGGCCGAAAAACTTGGTATCGGCGTGCATTTGGGACAGCAAGATGGTGATCTTAGTGAGGC
AACACAATCTTTAGCCCCGAATCAAATCATTGGTCGCACTTGTCATGGCGATATAACGCTAGTCAAAGCTGCGAAAACTG
AAGGCGCAAGCTATGCCGCCATGGGTGCAATCTTTGCTTCAACGACCAAGCCCAATGCTGCCACTATCTTACGCCAGCAA
CTGATAGAAGGCTGCCAGCAAGATATAAAAATATGCGTGATAGGCGGTTTAACGGCAGAAAACATCTCAGAGCTGGCAGG
GTTACCTATTGCTTATATAGCAATCGTTGGCGATATCATGGATTTACCTGTCCATCAAATCGCAACGCGTTGTCAGCAGT
GGCAGCAAGTACTTAATAAGTGGAATGTGCCTGCTGAATGA

Upstream 100 bases:

>100_bases
AAACGCGCAGTATCAAAAGACATTTATAATAAAAAGGCTGTTATGTGATATAACAGCCTTTTTATTATGTCAAAAACGTA
TTGATGTTAAGGAGCATTAC

Downstream 100 bases:

>100_bases
ATACTATTTTAAAATATAAGTACGAATTTATTCAGCGTGCTGCATACATAAAGTTGCATAAGGCAATGCTTGCAAGCGCT
GTGCTTCGATAGGCTCAGCG

Product: thiamine-phosphate diphosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase [H]

Number of amino acids: Translated: 226; Mature: 226

Protein sequence:

>226_residues
MIQSSVSTVSHTIPKLYLLTNDDEFSLLYQKLEAALATGLIALLQIRRKQILALPDGESVLYKEALQIVNLAKTYNVPVL
INDNIALAEKLGIGVHLGQQDGDLSEATQSLAPNQIIGRTCHGDITLVKAAKTEGASYAAMGAIFASTTKPNAATILRQQ
LIEGCQQDIKICVIGGLTAENISELAGLPIAYIAIVGDIMDLPVHQIATRCQQWQQVLNKWNVPAE

Sequences:

>Translated_226_residues
MIQSSVSTVSHTIPKLYLLTNDDEFSLLYQKLEAALATGLIALLQIRRKQILALPDGESVLYKEALQIVNLAKTYNVPVL
INDNIALAEKLGIGVHLGQQDGDLSEATQSLAPNQIIGRTCHGDITLVKAAKTEGASYAAMGAIFASTTKPNAATILRQQ
LIEGCQQDIKICVIGGLTAENISELAGLPIAYIAIVGDIMDLPVHQIATRCQQWQQVLNKWNVPAE
>Mature_226_residues
MIQSSVSTVSHTIPKLYLLTNDDEFSLLYQKLEAALATGLIALLQIRRKQILALPDGESVLYKEALQIVNLAKTYNVPVL
INDNIALAEKLGIGVHLGQQDGDLSEATQSLAPNQIIGRTCHGDITLVKAAKTEGASYAAMGAIFASTTKPNAATILRQQ
LIEGCQQDIKICVIGGLTAENISELAGLPIAYIAIVGDIMDLPVHQIATRCQQWQQVLNKWNVPAE

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6325042, Length=218, Percent_Identity=29.3577981651376, Blast_Score=65, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 24369; Mature: 24369

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQSSVSTVSHTIPKLYLLTNDDEFSLLYQKLEAALATGLIALLQIRRKQILALPDGESV
CCCCHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCHH
LYKEALQIVNLAKTYNVPVLINDNIALAEKLGIGVHLGQQDGDLSEATQSLAPNQIIGRT
HHHHHHHHHHHHHHCCCCEEEECCCHHHHHCCCEEEECCCCCCHHHHHHHCCCCHHHCCC
CHGDITLVKAAKTEGASYAAMGAIFASTTKPNAATILRQQLIEGCQQDIKICVIGGLTAE
CCCCEEEEEECCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCEEEEECCCCHH
NISELAGLPIAYIAIVGDIMDLPVHQIATRCQQWQQVLNKWNVPAE
HHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MIQSSVSTVSHTIPKLYLLTNDDEFSLLYQKLEAALATGLIALLQIRRKQILALPDGESV
CCCCHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCHH
LYKEALQIVNLAKTYNVPVLINDNIALAEKLGIGVHLGQQDGDLSEATQSLAPNQIIGRT
HHHHHHHHHHHHHHCCCCEEEECCCHHHHHCCCEEEECCCCCCHHHHHHHCCCCHHHCCC
CHGDITLVKAAKTEGASYAAMGAIFASTTKPNAATILRQQLIEGCQQDIKICVIGGLTAE
CCCCEEEEEECCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCEEEEECCCCHH
NISELAGLPIAYIAIVGDIMDLPVHQIATRCQQWQQVLNKWNVPAE
HHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA