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The map label for this gene is rlmN

Identifier: 93005478

GI number: 93005478

Start: 766375

End: 767616

Strand: Direct

Name: rlmN

Synonym: Pcryo_0648

Alternate gene names: 93005478

Gene position: 766375-767616 (Clockwise)

Preceding gene: 93005477

Following gene: 93005479

Centisome position: 25.05

GC content: 44.44

Gene sequence:

>1242_bases
ATGTCAACTGCTCAAAGCCCTATTCAAACTGACGTCCAACATGTACCTGCTAAAACTACTAAGAGTATAAAACTGGTAGA
TGAAGCGCAGGCGAAAACCAATCTACTAGGTATGACACAAGCGCAACTGGCGGATTACTTTAAGAGTATTGGCGAAAAGC
CGTTTCGCTCGACGCAAGTCATCAAGTGGATATATCAGCAGGGTGTCACTGATTTTGAGCAAATGACCAATCTGAGTAAA
TCTTTGCGTGATAAATTATCAGCCAATGCCTGTGTCATTCCGCCAAAAGTGATTCATCGTCAATATAGTGATGACGGCAC
GCGTAAATGGGTATTTGAAGTCACGGGTGGCTCGTTGGTCGAGACCGTACTGATTCCCGCAGATGATAGTAAATTAAACG
GTCGCAAAACTTTGTGTGTCTCTTCGCAAGTGGGCTGTGCGCTCGACTGTAGTTTTTGTAGTACGGGTAAGCAGGGCTTT
GAGCGCGATTTGACGGCGGCTGAGATTCTTGGACAATTATGGGTGGCAAATGCCTCTTATATGACCGATGAAAACGACAG
TTTAGAAAATGTTGACCATAGTTTATGGGAAAACAACGTCACCAACGTCGTCATGATGGGTATGGGTGAGCCGTTATTAA
ACTATCGTCCTGTTGTCAGCTCAATGGAATTGATGCTCAGTGACCATGCTTATGGTTTATCAAAGCGCCGTGTTACTTTA
TCGACTTCAGGTGTGGTGCCAAAAATGTATGAGTTGGCACAGGAGCTTGACGTGGCTTTAGCGATTTCATTGCACGCACC
GAATGACGAGCTGCGTAATGAGTTGGTACCGATTAATAAAAAATATCCATTAGAGCAGCTCATGGCGGCGGCGAGAAATT
ACGTTTTTGACGTGAATCCGCGCCACAAAAAACACGTGACCATTGAATATGTGATGTTAGATGGCGTCAATGATAGTAAT
GAACATGCTGAGCAATTGGTCGCATTGTTGGGTAATTTACCAAGTAAGATTAACCTGATACCGTTTAACCCGTTCCCACA
TGCAAATTATGATAAGTCGAGTAATAATCGTATTCATGCCTTTAGCAACATATTAAGTGAAGCAGGTTTTGTTTGTACCA
TTCGCCAAACGCGCGGCGATGATATTGACGCCGCTTGTGGTCAATTGGTTGGGCAAGTTGCTGACAGAACGCGTCGTTCA
GCCGCATGGCAGCAAAGCATCAAGGATCGCGAGAATAGTTAA

Upstream 100 bases:

>100_bases
ATAACGGATTGCTCATGCACTCATATATTGAGTACGTCTAGCAACACCCATCTCAACAATTGCATCGCTCATACCAGTAG
ATGCAAAAAGGTTATTTATT

Downstream 100 bases:

>100_bases
TTAGGTTAATAAAAATGACTGCTATAGCTATCTAACGTAGCTATAGACGAATTGATGTATTAAAGAGAATCCTACTATAG
TATTTTTTGTAGCCTCTACT

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase

Number of amino acids: Translated: 413; Mature: 412

Protein sequence:

>413_residues
MSTAQSPIQTDVQHVPAKTTKSIKLVDEAQAKTNLLGMTQAQLADYFKSIGEKPFRSTQVIKWIYQQGVTDFEQMTNLSK
SLRDKLSANACVIPPKVIHRQYSDDGTRKWVFEVTGGSLVETVLIPADDSKLNGRKTLCVSSQVGCALDCSFCSTGKQGF
ERDLTAAEILGQLWVANASYMTDENDSLENVDHSLWENNVTNVVMMGMGEPLLNYRPVVSSMELMLSDHAYGLSKRRVTL
STSGVVPKMYELAQELDVALAISLHAPNDELRNELVPINKKYPLEQLMAAARNYVFDVNPRHKKHVTIEYVMLDGVNDSN
EHAEQLVALLGNLPSKINLIPFNPFPHANYDKSSNNRIHAFSNILSEAGFVCTIRQTRGDDIDAACGQLVGQVADRTRRS
AAWQQSIKDRENS

Sequences:

>Translated_413_residues
MSTAQSPIQTDVQHVPAKTTKSIKLVDEAQAKTNLLGMTQAQLADYFKSIGEKPFRSTQVIKWIYQQGVTDFEQMTNLSK
SLRDKLSANACVIPPKVIHRQYSDDGTRKWVFEVTGGSLVETVLIPADDSKLNGRKTLCVSSQVGCALDCSFCSTGKQGF
ERDLTAAEILGQLWVANASYMTDENDSLENVDHSLWENNVTNVVMMGMGEPLLNYRPVVSSMELMLSDHAYGLSKRRVTL
STSGVVPKMYELAQELDVALAISLHAPNDELRNELVPINKKYPLEQLMAAARNYVFDVNPRHKKHVTIEYVMLDGVNDSN
EHAEQLVALLGNLPSKINLIPFNPFPHANYDKSSNNRIHAFSNILSEAGFVCTIRQTRGDDIDAACGQLVGQVADRTRRS
AAWQQSIKDRENS
>Mature_412_residues
STAQSPIQTDVQHVPAKTTKSIKLVDEAQAKTNLLGMTQAQLADYFKSIGEKPFRSTQVIKWIYQQGVTDFEQMTNLSKS
LRDKLSANACVIPPKVIHRQYSDDGTRKWVFEVTGGSLVETVLIPADDSKLNGRKTLCVSSQVGCALDCSFCSTGKQGFE
RDLTAAEILGQLWVANASYMTDENDSLENVDHSLWENNVTNVVMMGMGEPLLNYRPVVSSMELMLSDHAYGLSKRRVTLS
TSGVVPKMYELAQELDVALAISLHAPNDELRNELVPINKKYPLEQLMAAARNYVFDVNPRHKKHVTIEYVMLDGVNDSNE
HAEQLVALLGNLPSKINLIPFNPFPHANYDKSSNNRIHAFSNILSEAGFVCTIRQTRGDDIDAACGQLVGQVADRTRRSA
AWQQSIKDRENS

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family

Homologues:

Organism=Escherichia coli, GI1788865, Length=374, Percent_Identity=54.8128342245989, Blast_Score=406, Evalue=1e-114,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMN_PSYCK (Q1QD22)

Other databases:

- EMBL:   CP000323
- RefSeq:   YP_579915.1
- ProteinModelPortal:   Q1QD22
- STRING:   Q1QD22
- GeneID:   4035485
- GenomeReviews:   CP000323_GR
- KEGG:   pcr:Pcryo_0648
- NMPDR:   fig|335284.3.peg.11
- eggNOG:   COG0820
- HOGENOM:   HBG335345
- OMA:   TFCSTAQ
- PhylomeDB:   Q1QD22
- ProtClustDB:   CLSK830487
- BioCyc:   PCRY335284:PCRYO_0648-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01849
- InterPro:   IPR007197
- InterPro:   IPR004383
- PIRSF:   PIRSF006004
- TIGRFAMs:   TIGR00048

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.1.1.192

Molecular weight: Translated: 45909; Mature: 45777

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTAQSPIQTDVQHVPAKTTKSIKLVDEAQAKTNLLGMTQAQLADYFKSIGEKPFRSTQV
CCCCCCCHHHHHHHCCCCCCCCEEEHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHH
IKWIYQQGVTDFEQMTNLSKSLRDKLSANACVIPPKVIHRQYSDDGTRKWVFEVTGGSLV
HHHHHHHCCHHHHHHHHHHHHHHHHHCCCCEECCHHHHHHCCCCCCCEEEEEEECCCCEE
ETVLIPADDSKLNGRKTLCVSSQVGCALDCSFCSTGKQGFERDLTAAEILGQLWVANASY
EEEEEECCCCCCCCCEEEEEECCCCCEEECHHHHCCHHHHHHCHHHHHHHHHHHHCCCCC
MTDENDSLENVDHSLWENNVTNVVMMGMGEPLLNYRPVVSSMELMLSDHAYGLSKRRVTL
CCCCCCCHHHHHHHHHHCCCCEEEEECCCCCHHCCHHHHHHHHHHHHHCCCCCCCCEEEE
STSGVVPKMYELAQELDVALAISLHAPNDELRNELVPINKKYPLEQLMAAARNYVFDVNP
ECCCCCHHHHHHHHHCCEEEEEEEECCCHHHHHCCCCCCCCCCHHHHHHHHHCCEEECCC
RHKKHVTIEYVMLDGVNDSNEHAEQLVALLGNLPSKINLIPFNPFPHANYDKSSNNRIHA
CCCCEEEEEEEEEECCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCEEHH
FSNILSEAGFVCTIRQTRGDDIDAACGQLVGQVADRTRRSAAWQQSIKDRENS
HHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
STAQSPIQTDVQHVPAKTTKSIKLVDEAQAKTNLLGMTQAQLADYFKSIGEKPFRSTQV
CCCCCCHHHHHHHCCCCCCCCEEEHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHH
IKWIYQQGVTDFEQMTNLSKSLRDKLSANACVIPPKVIHRQYSDDGTRKWVFEVTGGSLV
HHHHHHHCCHHHHHHHHHHHHHHHHHCCCCEECCHHHHHHCCCCCCCEEEEEEECCCCEE
ETVLIPADDSKLNGRKTLCVSSQVGCALDCSFCSTGKQGFERDLTAAEILGQLWVANASY
EEEEEECCCCCCCCCEEEEEECCCCCEEECHHHHCCHHHHHHCHHHHHHHHHHHHCCCCC
MTDENDSLENVDHSLWENNVTNVVMMGMGEPLLNYRPVVSSMELMLSDHAYGLSKRRVTL
CCCCCCCHHHHHHHHHHCCCCEEEEECCCCCHHCCHHHHHHHHHHHHHCCCCCCCCEEEE
STSGVVPKMYELAQELDVALAISLHAPNDELRNELVPINKKYPLEQLMAAARNYVFDVNP
ECCCCCHHHHHHHHHCCEEEEEEEECCCHHHHHCCCCCCCCCCHHHHHHHHHCCEEECCC
RHKKHVTIEYVMLDGVNDSNEHAEQLVALLGNLPSKINLIPFNPFPHANYDKSSNNRIHA
CCCCEEEEEEEEEECCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCEEHH
FSNILSEAGFVCTIRQTRGDDIDAACGQLVGQVADRTRRSAAWQQSIKDRENS
HHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA