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The map label for this gene is purA [H]

Identifier: 93005475

GI number: 93005475

Start: 763322

End: 764611

Strand: Direct

Name: purA [H]

Synonym: Pcryo_0645

Alternate gene names: 93005475

Gene position: 763322-764611 (Clockwise)

Preceding gene: 93005474

Following gene: 93005476

Centisome position: 24.95

GC content: 45.5

Gene sequence:

>1290_bases
ATGGGTAAGAATGTCGTAGTTTTAGGTAGCCAATGGGGCGATGAAGGTAAGGGTAAAATCGTCGATTTACTAACCGAAAA
AGCCTCAGCAGTTGCACGCTTCCAAGGCGGACATAACGCTGGACATACGTTAGTTGTAGATGGTAAAACCACCGTCTTAC
ACCTAATCCCATCCGGTATTTTGCGTGAAGGCGTTACCTGCTTCATCGGTAATGGCGTGGTATTAGCACCTGATGCACTA
CTTAAAGAGATGAAAGGTTTAGAAGAGAATGGCGTTCCAGTTCGTGAGCGTCTACGTATTTCTCCTAACTGCCCATTAAT
CATGCCATATCATGTTGCCCTTGATCAAGCGCGTGAAGCCAAGCGTGGTAGTGGTAAAATCGGTACTACTGGTCGTGGTA
TTGGACCAGCTTATGAAGATAAAGTTGCGCGCCGTGCCATCAAGCTTGCCGACTTGTTCCGTGATGACTTAGAAGAAAAA
CTACGTAACTTAATCGAATATCATAACTTCCAATTGACTCAGTACTATAAAGTTGAAGCGATTGATTTTGATGAGACGCT
TAAGCTTTGTAAAGAGTGGAAAGAAGAAATCAGAGGCATGGTGACTGATGTCACTGAAGATTTGAACCAATTGCGCCTTG
CTGGCAAGAACCTAATGTTTGAAGGTGCACAAGGCACATTACTTGATATCGACCACGGTACGTATCCGTTTGTAACCAGC
TCAAGTGTAACCGCTGGTGGTGTATCTACTGGTACGGGTATCGGTCCATTATATTTGGATTATGTCCTTGGTATCACGAA
AGCCTATACCACCCGTGTTGGTAGTGGTCCATTCCCAACTGAATTGTTTGATGATGTTGGTGCGCATTTGGCTAAAGTCG
GTCATGAGTTTGGTGCGACTACTGGTCGCGCGCGTCGCTGTGGTTGGTTTGATGCTGAAGCATTACGCCGTGCAGTGGTA
CTTAACTCTATGTCAGGTATCTGCCTAACCAAGCTTGACGTGTTAGATGGTCTAGAAGAATTGCTAATCGGTGTGGGTTA
TAACTTACCTGAGACTGAGTGTGCAGGCGCGCATGATGCAGAGTTCTACGAAGCGGTAACGCCAAAGTATGAAACGCTAC
AAGGCTGGAGCGAATCAACGGTCGGTATCACTAACTACGATGAGCTGCCTGAAAACGCCAAAATATACATCAAACGTATC
GAAGCATTGATTGGTTGTCCAGTTGATATCATCTCAACCGGTCCTGATCGTGAAGAGACCATTGTATTGCGTGATCCGTA
CGATGCTTAA

Upstream 100 bases:

>100_bases
TACTTTGTTTAAAGTATCGTTTTTAGCATTACTTTCGTTTTGATAGGTAGTTTTAAACACTTATTGCACACACCTCAATA
TATTAAGGTAAGGATTTATC

Downstream 100 bases:

>100_bases
TTGGTAAAAAAGTTACTTAGTAACAATAACCTCCATTGGCTAAATGCAATGGGGGTTTTTTTACGGCATTTTTTTAAAAT
ATTGGCACTTCTTAATTACA

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase [H]

Number of amino acids: Translated: 429; Mature: 428

Protein sequence:

>429_residues
MGKNVVVLGSQWGDEGKGKIVDLLTEKASAVARFQGGHNAGHTLVVDGKTTVLHLIPSGILREGVTCFIGNGVVLAPDAL
LKEMKGLEENGVPVRERLRISPNCPLIMPYHVALDQAREAKRGSGKIGTTGRGIGPAYEDKVARRAIKLADLFRDDLEEK
LRNLIEYHNFQLTQYYKVEAIDFDETLKLCKEWKEEIRGMVTDVTEDLNQLRLAGKNLMFEGAQGTLLDIDHGTYPFVTS
SSVTAGGVSTGTGIGPLYLDYVLGITKAYTTRVGSGPFPTELFDDVGAHLAKVGHEFGATTGRARRCGWFDAEALRRAVV
LNSMSGICLTKLDVLDGLEELLIGVGYNLPETECAGAHDAEFYEAVTPKYETLQGWSESTVGITNYDELPENAKIYIKRI
EALIGCPVDIISTGPDREETIVLRDPYDA

Sequences:

>Translated_429_residues
MGKNVVVLGSQWGDEGKGKIVDLLTEKASAVARFQGGHNAGHTLVVDGKTTVLHLIPSGILREGVTCFIGNGVVLAPDAL
LKEMKGLEENGVPVRERLRISPNCPLIMPYHVALDQAREAKRGSGKIGTTGRGIGPAYEDKVARRAIKLADLFRDDLEEK
LRNLIEYHNFQLTQYYKVEAIDFDETLKLCKEWKEEIRGMVTDVTEDLNQLRLAGKNLMFEGAQGTLLDIDHGTYPFVTS
SSVTAGGVSTGTGIGPLYLDYVLGITKAYTTRVGSGPFPTELFDDVGAHLAKVGHEFGATTGRARRCGWFDAEALRRAVV
LNSMSGICLTKLDVLDGLEELLIGVGYNLPETECAGAHDAEFYEAVTPKYETLQGWSESTVGITNYDELPENAKIYIKRI
EALIGCPVDIISTGPDREETIVLRDPYDA
>Mature_428_residues
GKNVVVLGSQWGDEGKGKIVDLLTEKASAVARFQGGHNAGHTLVVDGKTTVLHLIPSGILREGVTCFIGNGVVLAPDALL
KEMKGLEENGVPVRERLRISPNCPLIMPYHVALDQAREAKRGSGKIGTTGRGIGPAYEDKVARRAIKLADLFRDDLEEKL
RNLIEYHNFQLTQYYKVEAIDFDETLKLCKEWKEEIRGMVTDVTEDLNQLRLAGKNLMFEGAQGTLLDIDHGTYPFVTSS
SVTAGGVSTGTGIGPLYLDYVLGITKAYTTRVGSGPFPTELFDDVGAHLAKVGHEFGATTGRARRCGWFDAEALRRAVVL
NSMSGICLTKLDVLDGLEELLIGVGYNLPETECAGAHDAEFYEAVTPKYETLQGWSESTVGITNYDELPENAKIYIKRIE
ALIGCPVDIISTGPDREETIVLRDPYDA

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP [H]

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family [H]

Homologues:

Organism=Homo sapiens, GI22748717, Length=426, Percent_Identity=44.8356807511737, Blast_Score=328, Evalue=4e-90,
Organism=Homo sapiens, GI34577063, Length=428, Percent_Identity=43.2242990654206, Blast_Score=307, Evalue=1e-83,
Organism=Homo sapiens, GI40316944, Length=397, Percent_Identity=43.0730478589421, Blast_Score=287, Evalue=2e-77,
Organism=Escherichia coli, GI1790620, Length=432, Percent_Identity=64.5833333333333, Blast_Score=568, Evalue=1e-163,
Organism=Caenorhabditis elegans, GI25146553, Length=430, Percent_Identity=44.1860465116279, Blast_Score=336, Evalue=1e-92,
Organism=Caenorhabditis elegans, GI25146555, Length=430, Percent_Identity=44.1860465116279, Blast_Score=336, Evalue=2e-92,
Organism=Saccharomyces cerevisiae, GI6324109, Length=429, Percent_Identity=41.7249417249417, Blast_Score=326, Evalue=4e-90,
Organism=Drosophila melanogaster, GI21356033, Length=421, Percent_Identity=40.855106888361, Blast_Score=317, Evalue=1e-86,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018220
- InterPro:   IPR001114 [H]

Pfam domain/function: PF00709 Adenylsucc_synt [H]

EC number: =6.3.4.4 [H]

Molecular weight: Translated: 46806; Mature: 46675

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1 ; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKNVVVLGSQWGDEGKGKIVDLLTEKASAVARFQGGHNAGHTLVVDGKTTVLHLIPSGI
CCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCEEEEECCHHH
LREGVTCFIGNGVVLAPDALLKEMKGLEENGVPVRERLRISPNCPLIMPYHVALDQAREA
HHCCCEEEECCCEEECCHHHHHHHHCCCCCCCCHHHHEEECCCCCEEEEHHHHHHHHHHH
KRGSGKIGTTGRGIGPAYEDKVARRAIKLADLFRDDLEEKLRNLIEYHNFQLTQYYKVEA
HCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEE
IDFDETLKLCKEWKEEIRGMVTDVTEDLNQLRLAGKNLMFEGAQGTLLDIDHGTYPFVTS
ECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCEEECCCCCCCEEEC
SSVTAGGVSTGTGIGPLYLDYVLGITKAYTTRVGSGPFPTELFDDVGAHLAKVGHEFGAT
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
TGRARRCGWFDAEALRRAVVLNSMSGICLTKLDVLDGLEELLIGVGYNLPETECAGAHDA
CCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCCCCH
EFYEAVTPKYETLQGWSESTVGITNYDELPENAKIYIKRIEALIGCPVDIISTGPDREET
HHHHHHCCCHHHHCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHCCCHHEECCCCCCCCE
IVLRDPYDA
EEEECCCCC
>Mature Secondary Structure 
GKNVVVLGSQWGDEGKGKIVDLLTEKASAVARFQGGHNAGHTLVVDGKTTVLHLIPSGI
CCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCEEEEECCHHH
LREGVTCFIGNGVVLAPDALLKEMKGLEENGVPVRERLRISPNCPLIMPYHVALDQAREA
HHCCCEEEECCCEEECCHHHHHHHHCCCCCCCCHHHHEEECCCCCEEEEHHHHHHHHHHH
KRGSGKIGTTGRGIGPAYEDKVARRAIKLADLFRDDLEEKLRNLIEYHNFQLTQYYKVEA
HCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEE
IDFDETLKLCKEWKEEIRGMVTDVTEDLNQLRLAGKNLMFEGAQGTLLDIDHGTYPFVTS
ECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCEEECCCCCCCEEEC
SSVTAGGVSTGTGIGPLYLDYVLGITKAYTTRVGSGPFPTELFDDVGAHLAKVGHEFGAT
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
TGRARRCGWFDAEALRRAVVLNSMSGICLTKLDVLDGLEELLIGVGYNLPETECAGAHDA
CCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCCCCH
EFYEAVTPKYETLQGWSESTVGITNYDELPENAKIYIKRIEALIGCPVDIISTGPDREET
HHHHHHCCCHHHHCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHCCCHHEECCCCCCCCE
IVLRDPYDA
EEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA