Click here to switch to the map view.

The map label for this gene is bioB [H]

Identifier: 93005192

GI number: 93005192

Start: 438997

End: 440292

Strand: Direct

Name: bioB [H]

Synonym: Pcryo_0361

Alternate gene names: 93005192

Gene position: 438997-440292 (Clockwise)

Preceding gene: 93005191

Following gene: 93005193

Centisome position: 14.35

GC content: 45.52

Gene sequence:

>1296_bases
ATGACAACTGTATCAACCAACGATTTTGACCAGCAGCACCTTTGGCACCCGTATGCCAGCCTACCACCGACTTATCCCAA
TATCGTGATTGACCGTGCCGAAGGTATTTATATCGTTACTGAAGATGGTACGCGCCTGATTGATGGTATGTCGTCATGGT
GGGCAAGCGTGCACGGTTATAATCATCCTAAGTTAAATGCGGCGATGATTGAACAATTGGGCAAAATGGCGCACGTCATG
TTTGGTGGTTTAACCCATCAGCCAGCGATAGACTTGGGCAAAAAACTACTCTCAATCGTGCCCGCTGGACTAGACGCAAT
ATTTTATGCTGATAGTGGCAGCATTGCCGTAGAAGTCGCATTAAAAATGGCGCTGCAATATCAGATTGCCGCCAAGCGTC
CTAGTAAATGTCAATTTGCCTCGACTCATTCTGGCTATTATGGCGATACGTGGCATGCGATGAGTGTCTGCGATCCTATT
AACGGCATGCATAGCCTTTATGGCAAGCAGTTGCCGATGCAGCATTTTGTCGCAGCACCACCAATGGGATTTGAGCGTGA
TTTAACCCAAAGTGAGCGTGAGGCATTAACTGAGTTTTTTGTCAAAAATAGCGATAAGCTTGCTGGATTTATCATCGAGC
CGATTATTCAAGGCGCAGGCGGCATGCGCTTTTATAGTCCTCAGTATTTGCAACTGTTACGTAAGCTGTGTGATGAATAT
GATGTGCTGTTGATTGCCGATGAAATTGCCACCGGTTTTGGGCGTAGTGGCAAGTTATTTGCTTGTGAGCATGCCGCTAT
TAGCCCTGATATTATGACAATTGGTAAGGCACTGACTGGCGGTTATATGACTTTTGCCGCGACCCTGTCTACACGAGAAA
TAGCTGACACCATTAGCCAAAGTGATTACCCGGCACTGATGCACGGTCCCACCTTTATGGGTAATCCGCTGGCATGTGCC
GTTGCTTGTGCCTCAATTGACTTAATAGTGTCCTATGATATTGAAGCGCGTACCGAAAATATGCAAGCCATTATGAATGA
GCAACTTGCGCCAGCTGTGAGTTTAGAAGGTGTTAAGGAAGTGCGTTGTTTAGGCGCAGTTGCCGTCATTGAGCTAAATG
AAGCGGTTGATATGCCTATCTTTCAAACGTTGCTCATTAATAATGGCATCTGGGTCAGACCCTTTGGGAAATTAGTTTAT
ATTATGCCGCCCTATGTGATTACCGATGATGAACTTACCACTCTTTGCCAAGCGTTATTAAAAGTGGTCAGTAGTTATTT
AACGCGAAAGGATTAA

Upstream 100 bases:

>100_bases
GGTTATTTGCCCTGTCGATTGTATTCCTCATGATCCCAATCATATCGAAACCGAAAGTGATTTGATGTCTAAATACAAAC
GCATTACTGGTCAATAAAAT

Downstream 100 bases:

>100_bases
TGATGAGCGTGCTCTTTATATCTGGTATCGATACCGACATTGGCAAAACTTATGCTACCGGTATGATTGCCAAAGCGCTG
ATGCAGCACGGTGTTAATGT

Product: adenosylmethionine-8-amino-7-oxononanoate aminotransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 431; Mature: 430

Protein sequence:

>431_residues
MTTVSTNDFDQQHLWHPYASLPPTYPNIVIDRAEGIYIVTEDGTRLIDGMSSWWASVHGYNHPKLNAAMIEQLGKMAHVM
FGGLTHQPAIDLGKKLLSIVPAGLDAIFYADSGSIAVEVALKMALQYQIAAKRPSKCQFASTHSGYYGDTWHAMSVCDPI
NGMHSLYGKQLPMQHFVAAPPMGFERDLTQSEREALTEFFVKNSDKLAGFIIEPIIQGAGGMRFYSPQYLQLLRKLCDEY
DVLLIADEIATGFGRSGKLFACEHAAISPDIMTIGKALTGGYMTFAATLSTREIADTISQSDYPALMHGPTFMGNPLACA
VACASIDLIVSYDIEARTENMQAIMNEQLAPAVSLEGVKEVRCLGAVAVIELNEAVDMPIFQTLLINNGIWVRPFGKLVY
IMPPYVITDDELTTLCQALLKVVSSYLTRKD

Sequences:

>Translated_431_residues
MTTVSTNDFDQQHLWHPYASLPPTYPNIVIDRAEGIYIVTEDGTRLIDGMSSWWASVHGYNHPKLNAAMIEQLGKMAHVM
FGGLTHQPAIDLGKKLLSIVPAGLDAIFYADSGSIAVEVALKMALQYQIAAKRPSKCQFASTHSGYYGDTWHAMSVCDPI
NGMHSLYGKQLPMQHFVAAPPMGFERDLTQSEREALTEFFVKNSDKLAGFIIEPIIQGAGGMRFYSPQYLQLLRKLCDEY
DVLLIADEIATGFGRSGKLFACEHAAISPDIMTIGKALTGGYMTFAATLSTREIADTISQSDYPALMHGPTFMGNPLACA
VACASIDLIVSYDIEARTENMQAIMNEQLAPAVSLEGVKEVRCLGAVAVIELNEAVDMPIFQTLLINNGIWVRPFGKLVY
IMPPYVITDDELTTLCQALLKVVSSYLTRKD
>Mature_430_residues
TTVSTNDFDQQHLWHPYASLPPTYPNIVIDRAEGIYIVTEDGTRLIDGMSSWWASVHGYNHPKLNAAMIEQLGKMAHVMF
GGLTHQPAIDLGKKLLSIVPAGLDAIFYADSGSIAVEVALKMALQYQIAAKRPSKCQFASTHSGYYGDTWHAMSVCDPIN
GMHSLYGKQLPMQHFVAAPPMGFERDLTQSEREALTEFFVKNSDKLAGFIIEPIIQGAGGMRFYSPQYLQLLRKLCDEYD
VLLIADEIATGFGRSGKLFACEHAAISPDIMTIGKALTGGYMTFAATLSTREIADTISQSDYPALMHGPTFMGNPLACAV
ACASIDLIVSYDIEARTENMQAIMNEQLAPAVSLEGVKEVRCLGAVAVIELNEAVDMPIFQTLLINNGIWVRPFGKLVYI
MPPYVITDDELTTLCQALLKVVSSYLTRKD

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism [H]

COG id: COG0161

COG function: function code H; Adenosylmethionine-8-amino-7-oxononanoate aminotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family [H]

Homologues:

Organism=Homo sapiens, GI4557809, Length=437, Percent_Identity=28.8329519450801, Blast_Score=148, Evalue=1e-35,
Organism=Homo sapiens, GI284507298, Length=326, Percent_Identity=30.3680981595092, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI13994255, Length=437, Percent_Identity=26.3157894736842, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI37574042, Length=428, Percent_Identity=25.2336448598131, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI226442705, Length=430, Percent_Identity=24.6511627906977, Blast_Score=86, Evalue=9e-17,
Organism=Homo sapiens, GI226442709, Length=397, Percent_Identity=24.6851385390428, Blast_Score=84, Evalue=2e-16,
Organism=Escherichia coli, GI1786991, Length=423, Percent_Identity=51.5366430260047, Blast_Score=450, Evalue=1e-127,
Organism=Escherichia coli, GI145693181, Length=403, Percent_Identity=31.7617866004963, Blast_Score=164, Evalue=1e-41,
Organism=Escherichia coli, GI1789016, Length=355, Percent_Identity=30.1408450704225, Blast_Score=142, Evalue=3e-35,
Organism=Escherichia coli, GI1789759, Length=312, Percent_Identity=32.0512820512821, Blast_Score=141, Evalue=1e-34,
Organism=Escherichia coli, GI1788044, Length=402, Percent_Identity=28.8557213930348, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1787560, Length=435, Percent_Identity=26.2068965517241, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI1786349, Length=378, Percent_Identity=24.8677248677249, Blast_Score=96, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI25144271, Length=433, Percent_Identity=25.4041570438799, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI25144274, Length=221, Percent_Identity=31.6742081447964, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI32564660, Length=442, Percent_Identity=26.6968325791855, Blast_Score=115, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17541228, Length=399, Percent_Identity=24.0601503759398, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71992977, Length=374, Percent_Identity=23.5294117647059, Blast_Score=80, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6324386, Length=459, Percent_Identity=41.8300653594771, Blast_Score=358, Evalue=1e-99,
Organism=Saccharomyces cerevisiae, GI6323470, Length=439, Percent_Identity=26.1958997722096, Blast_Score=128, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6324432, Length=382, Percent_Identity=28.2722513089005, Blast_Score=115, Evalue=1e-26,
Organism=Drosophila melanogaster, GI21357415, Length=417, Percent_Identity=27.8177458033573, Blast_Score=146, Evalue=2e-35,
Organism=Drosophila melanogaster, GI28574759, Length=422, Percent_Identity=24.8815165876777, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI161085790, Length=372, Percent_Identity=25.5376344086022, Blast_Score=92, Evalue=9e-19,
Organism=Drosophila melanogaster, GI21356575, Length=387, Percent_Identity=25.0645994832041, Blast_Score=76, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR005814
- InterPro:   IPR005815
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR007197 [H]

Pfam domain/function: PF00202 Aminotran_3; PF06968 BATS; PF04055 Radical_SAM [H]

EC number: =2.8.1.6 [H]

Molecular weight: Translated: 47337; Mature: 47206

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTVSTNDFDQQHLWHPYASLPPTYPNIVIDRAEGIYIVTEDGTRLIDGMSSWWASVHGY
CCCCCCCCCCHHHHCCCCCCCCCCCCCEEEECCCCEEEEECCCCHHHHHHHHHHHHHCCC
NHPKLNAAMIEQLGKMAHVMFGGLTHQPAIDLGKKLLSIVPAGLDAIFYADSGSIAVEVA
CCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCEEHHHH
LKMALQYQIAAKRPSKCQFASTHSGYYGDTWHAMSVCDPINGMHSLYGKQLPMQHFVAAP
HHHHHHHHHHCCCCCCCEEECCCCCCCCCCCHHHHHHCCCHHHHHHHHCCCCHHHHHCCC
PMGFERDLTQSEREALTEFFVKNSDKLAGFIIEPIIQGAGGMRFYSPQYLQLLRKLCDEY
CCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCC
DVLLIADEIATGFGRSGKLFACEHAAISPDIMTIGKALTGGYMTFAATLSTREIADTISQ
CEEEEEHHHHHCCCCCCCEEEECCCCCCCHHHHHHHHHCCCCEEEEEHHHHHHHHHHHCC
SDYPALMHGPTFMGNPLACAVACASIDLIVSYDIEARTENMQAIMNEQLAPAVSLEGVKE
CCCCEEEECCCCCCCHHHHHHHHHHEEEEEEECCHHHHHHHHHHHHHHCCCHHHHCHHHH
VRCLGAVAVIELNEAVDMPIFQTLLINNGIWVRPFGKLVYIMPPYVITDDELTTLCQALL
HHHHCEEEEEEECCCCCCHHHHHHHHCCCEEEEECCCEEEEECCEEEECHHHHHHHHHHH
KVVSSYLTRKD
HHHHHHHHCCC
>Mature Secondary Structure 
TTVSTNDFDQQHLWHPYASLPPTYPNIVIDRAEGIYIVTEDGTRLIDGMSSWWASVHGY
CCCCCCCCCHHHHCCCCCCCCCCCCCEEEECCCCEEEEECCCCHHHHHHHHHHHHHCCC
NHPKLNAAMIEQLGKMAHVMFGGLTHQPAIDLGKKLLSIVPAGLDAIFYADSGSIAVEVA
CCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCEEHHHH
LKMALQYQIAAKRPSKCQFASTHSGYYGDTWHAMSVCDPINGMHSLYGKQLPMQHFVAAP
HHHHHHHHHHCCCCCCCEEECCCCCCCCCCCHHHHHHCCCHHHHHHHHCCCCHHHHHCCC
PMGFERDLTQSEREALTEFFVKNSDKLAGFIIEPIIQGAGGMRFYSPQYLQLLRKLCDEY
CCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCC
DVLLIADEIATGFGRSGKLFACEHAAISPDIMTIGKALTGGYMTFAATLSTREIADTISQ
CEEEEEHHHHHCCCCCCCEEEECCCCCCCHHHHHHHHHCCCCEEEEEHHHHHHHHHHHCC
SDYPALMHGPTFMGNPLACAVACASIDLIVSYDIEARTENMQAIMNEQLAPAVSLEGVKE
CCCCEEEECCCCCCCHHHHHHHHHHEEEEEEECCHHHHHHHHHHHHHHCCCHHHHCHHHH
VRCLGAVAVIELNEAVDMPIFQTLLINNGIWVRPFGKLVYIMPPYVITDDELTTLCQALL
HHHHCEEEEEEECCCCCCHHHHHHHHCCCEEEEECCCEEEEECCEEEECHHHHHHHHHHH
KVVSSYLTRKD
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA