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The map label for this gene is ligA [H]

Identifier: 93005182

GI number: 93005182

Start: 427681

End: 429756

Strand: Direct

Name: ligA [H]

Synonym: Pcryo_0351

Alternate gene names: 93005182

Gene position: 427681-429756 (Clockwise)

Preceding gene: 93005181

Following gene: 93005183

Centisome position: 13.98

GC content: 49.71

Gene sequence:

>2076_bases
ATGACTGACCCTATCGCACCGCTTACCTCTAAAGACATCAATACTGCAAAATCTGCAGCAAACGAAGATGCTATCGTGGC
TCAAATGCGCACGTTTATTGATGCCCTTAAGAAGCACAATTATGCCTATTATGTGCTCGACAATCCCATCTTAGAAGACA
GCGAATACGATCAGTTGCGTCGCTCTTTATTGGAGCTTGAAGAAGAATATCCCGATTTAATACAGCCGGATAGCCCAATC
AATCAAGTTGGCGATATGCCGCTATCGGCATTTACGCAAGTCACCCATGATATTCCGATGCTGTCGCTTGGCAACGTCTT
TGAATACGATGATTTGCGTGATTTTATGCGCCGCGTTAATGACCGCTTAAGCGTGGCTCAGCAAAGCCCTGAATACGAGA
TGGAGCTAAAGCTAGATGGCTTGGCGGTATCGCTCAAATATGCTTATGGCAAGTTTGTCCAAGCGGTGACACGCGGTGAT
GGACAAACGGGCGAGGATATTACCCAAAATGCCAAAACTATCCGTAACTTGCCGCTATGGATTCCTGCTGCAAGCGATAT
TGAGCTATTAGAAGTACGCGGTGAAGTGCTGATGCCGAAAGCGGGCTTTGAGCGTTTGAACCGCCTTGCTGAAGAAAAGG
GCGACAAAACTTTTGCCAATCCACGCAATGCTGCCGCTGGAAGTTTAAGACAACTTGATCCCGCCATTGCCGCTAGCCGT
CCGCTCGCGTTTTATTGCTATTCTGTCAATCAAGGGTTACCTGAGCATATCAAAACCCAATCAGCAGCGCTTGCTTGGCT
AAAAACCATTGGCTTTACCGTCAGTGCAGTTGAGGTAGTACAAAATCCACGCGAGGCTCAAGCCTATTATGAATCGGTAA
AGGAGACACGTGGCGATCTGCCGTTCGAGATTGATGGTATGGTCATCAAGGTCAATAGCTTGGCGTTGCAGCAACAGCTT
GGTTTTTTATCGCGTGAGCCGCGCTGGGCAACCGCTTATAAATTCCCTGCCGAAACCGTCATGACACGCTTGCATGCTAT
CGAGTGGCAAGTCGGACGCACTGGCGCGATTACTCCAGTCGGTAAATTAGAACCCGTGAAAGTCGGTGGCGTCACGGTCA
GCAATGTCACGCTGCATAACTTTGGTGAGATTCAGCGATTGGATGTGCGTGCAGGCGATATGGTCAGCGTCCATCGTGCA
GGGGATGTGATACCTAAAGTCACCCGCGTTTGGACAGATCAGCGTCCAGAAAACTCTGAACCCGTTAAGTTGCCATCGAC
TTGCCCAGTATGCGACTCGCCAGTGGTGCTGCCCAAAGATGAAGCATTGGCGCGTTGCACCGGTGGGCTATTTTGTCCCG
CGCAGCAGGTCGAGGCGCTCATTCACTTTGTCTCGCGTCGGGCGATGGATATCGATGGCTTAGGCGCAAGTTGGCTGATT
AGCTTTTTTGAGCACGGTTTGGTCAAGACCGTTGCCGATATCTATCAGTTGCACAATCATCAAGAGGAGCTGATTACGCT
TGAGAAACTGGGCGAAAAATCTGTTCAAAATATCCTCAGCGCTATCGAAGCCAGTAAGCAGACGACGCTCGCACGCTTTA
TCTATGCGCTGGGTATTCGCGGTGTCGGTGAGACGACGGCGCAAAACCTCGCTCAACAATTTGGCGATCTTGATGCCTTA
ATGTCTGCCAGTATCGAAAAACTGCTACTCACGCCAGATGTGGGTGCTATTACCGCTGAACTTACTTATAAATTCTTTCG
CGCACCGCATAATATCGAAGTCATCAATGCATTACGCGAGGCAGGCGTGCATTGGGATAAGGTTGAGCAGGTCGCGTCAG
AAGGATTGCCGCTCGATGGTCAAACATGGGTGATTACTGGCGCGCTTGATAGCATGGCACGCGATGAAGCCAAAGCCAAA
CTGCAAGCGCTTGGTGCCAAAGTATCTGGTAGCATCTCTGCAAAAACCACGGCGTTACTGGCAGGTGATAAGGCTGGCTC
AAAAATGGCAAAAGCTGAAAAACTGGGCGTTAAAGTAGTGGGTGAAGAAGAGTTTTTGGTGTTGGTTGGGGAGTAG

Upstream 100 bases:

>100_bases
TACTTTTGCAAACCAGTCATTTTCATTAAAAGTTTATCCGAGTATCTTGCAAACTTGTTATCATATCGACATCTTGAATT
GCAAAGAAAAAACACCGACT

Downstream 100 bases:

>100_bases
AGTATGAGTGTCGACAATATGGAAAATTTAGACGAAGAAACTCGTAAAAAAATTGAAAAGTTACTTAAGGTGACGCGTGA
GAAAGATGGTAATGAGAGTT

Product: DNA ligase, NAD-dependent

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+] [H]

Number of amino acids: Translated: 691; Mature: 690

Protein sequence:

>691_residues
MTDPIAPLTSKDINTAKSAANEDAIVAQMRTFIDALKKHNYAYYVLDNPILEDSEYDQLRRSLLELEEEYPDLIQPDSPI
NQVGDMPLSAFTQVTHDIPMLSLGNVFEYDDLRDFMRRVNDRLSVAQQSPEYEMELKLDGLAVSLKYAYGKFVQAVTRGD
GQTGEDITQNAKTIRNLPLWIPAASDIELLEVRGEVLMPKAGFERLNRLAEEKGDKTFANPRNAAAGSLRQLDPAIAASR
PLAFYCYSVNQGLPEHIKTQSAALAWLKTIGFTVSAVEVVQNPREAQAYYESVKETRGDLPFEIDGMVIKVNSLALQQQL
GFLSREPRWATAYKFPAETVMTRLHAIEWQVGRTGAITPVGKLEPVKVGGVTVSNVTLHNFGEIQRLDVRAGDMVSVHRA
GDVIPKVTRVWTDQRPENSEPVKLPSTCPVCDSPVVLPKDEALARCTGGLFCPAQQVEALIHFVSRRAMDIDGLGASWLI
SFFEHGLVKTVADIYQLHNHQEELITLEKLGEKSVQNILSAIEASKQTTLARFIYALGIRGVGETTAQNLAQQFGDLDAL
MSASIEKLLLTPDVGAITAELTYKFFRAPHNIEVINALREAGVHWDKVEQVASEGLPLDGQTWVITGALDSMARDEAKAK
LQALGAKVSGSISAKTTALLAGDKAGSKMAKAEKLGVKVVGEEEFLVLVGE

Sequences:

>Translated_691_residues
MTDPIAPLTSKDINTAKSAANEDAIVAQMRTFIDALKKHNYAYYVLDNPILEDSEYDQLRRSLLELEEEYPDLIQPDSPI
NQVGDMPLSAFTQVTHDIPMLSLGNVFEYDDLRDFMRRVNDRLSVAQQSPEYEMELKLDGLAVSLKYAYGKFVQAVTRGD
GQTGEDITQNAKTIRNLPLWIPAASDIELLEVRGEVLMPKAGFERLNRLAEEKGDKTFANPRNAAAGSLRQLDPAIAASR
PLAFYCYSVNQGLPEHIKTQSAALAWLKTIGFTVSAVEVVQNPREAQAYYESVKETRGDLPFEIDGMVIKVNSLALQQQL
GFLSREPRWATAYKFPAETVMTRLHAIEWQVGRTGAITPVGKLEPVKVGGVTVSNVTLHNFGEIQRLDVRAGDMVSVHRA
GDVIPKVTRVWTDQRPENSEPVKLPSTCPVCDSPVVLPKDEALARCTGGLFCPAQQVEALIHFVSRRAMDIDGLGASWLI
SFFEHGLVKTVADIYQLHNHQEELITLEKLGEKSVQNILSAIEASKQTTLARFIYALGIRGVGETTAQNLAQQFGDLDAL
MSASIEKLLLTPDVGAITAELTYKFFRAPHNIEVINALREAGVHWDKVEQVASEGLPLDGQTWVITGALDSMARDEAKAK
LQALGAKVSGSISAKTTALLAGDKAGSKMAKAEKLGVKVVGEEEFLVLVGE
>Mature_690_residues
TDPIAPLTSKDINTAKSAANEDAIVAQMRTFIDALKKHNYAYYVLDNPILEDSEYDQLRRSLLELEEEYPDLIQPDSPIN
QVGDMPLSAFTQVTHDIPMLSLGNVFEYDDLRDFMRRVNDRLSVAQQSPEYEMELKLDGLAVSLKYAYGKFVQAVTRGDG
QTGEDITQNAKTIRNLPLWIPAASDIELLEVRGEVLMPKAGFERLNRLAEEKGDKTFANPRNAAAGSLRQLDPAIAASRP
LAFYCYSVNQGLPEHIKTQSAALAWLKTIGFTVSAVEVVQNPREAQAYYESVKETRGDLPFEIDGMVIKVNSLALQQQLG
FLSREPRWATAYKFPAETVMTRLHAIEWQVGRTGAITPVGKLEPVKVGGVTVSNVTLHNFGEIQRLDVRAGDMVSVHRAG
DVIPKVTRVWTDQRPENSEPVKLPSTCPVCDSPVVLPKDEALARCTGGLFCPAQQVEALIHFVSRRAMDIDGLGASWLIS
FFEHGLVKTVADIYQLHNHQEELITLEKLGEKSVQNILSAIEASKQTTLARFIYALGIRGVGETTAQNLAQQFGDLDALM
SASIEKLLLTPDVGAITAELTYKFFRAPHNIEVINALREAGVHWDKVEQVASEGLPLDGQTWVITGALDSMARDEAKAKL
QALGAKVSGSISAKTTALLAGDKAGSKMAKAEKLGVKVVGEEEFLVLVGE

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain [H]

Homologues:

Organism=Escherichia coli, GI1788750, Length=675, Percent_Identity=51.7037037037037, Blast_Score=660, Evalue=0.0,
Organism=Escherichia coli, GI87082305, Length=519, Percent_Identity=24.4701348747592, Blast_Score=97, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149 [H]

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD [H]

EC number: =6.5.1.2 [H]

Molecular weight: Translated: 75897; Mature: 75766

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS50172 BRCT ; PS01055 DNA_LIGASE_N1 ; PS01056 DNA_LIGASE_N2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDPIAPLTSKDINTAKSAANEDAIVAQMRTFIDALKKHNYAYYVLDNPILEDSEYDQLR
CCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHH
RSLLELEEEYPDLIQPDSPINQVGDMPLSAFTQVTHDIPMLSLGNVFEYDDLRDFMRRVN
HHHHHHHHHCCCCCCCCCCHHHHCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHH
DRLSVAQQSPEYEMELKLDGLAVSLKYAYGKFVQAVTRGDGQTGEDITQNAKTIRNLPLW
HHHHHHHCCCCCEEEEEECCEEEEEHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCEE
IPAASDIELLEVRGEVLMPKAGFERLNRLAEEKGDKTFANPRNAAAGSLRQLDPAIAASR
ECCCCCCCEEEECCCEECCCHHHHHHHHHHHHCCCCCCCCCCCHHHCCHHHCCHHHHCCC
PLAFYCYSVNQGLPEHIKTQSAALAWLKTIGFTVSAVEVVQNPREAQAYYESVKETRGDL
CEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHCCCC
PFEIDGMVIKVNSLALQQQLGFLSREPRWATAYKFPAETVMTRLHAIEWQVGRTGAITPV
CEEECCEEEEEHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHEECCCCCCCCCC
GKLEPVKVGGVTVSNVTLHNFGEIQRLDVRAGDMVSVHRAGDVIPKVTRVWTDQRPENSE
CCCCEEEECCEEEECEEECCCCCEEEEEECCCCEEEEHHHCCHHHHHHHHHCCCCCCCCC
PVKLPSTCPVCDSPVVLPKDEALARCTGGLFCPAQQVEALIHFVSRRAMDIDGLGASWLI
CEECCCCCCCCCCCEECCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHH
SFFEHGLVKTVADIYQLHNHQEELITLEKLGEKSVQNILSAIEASKQTTLARFIYALGIR
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GVGETTAQNLAQQFGDLDALMSASIEKLLLTPDVGAITAELTYKFFRAPHNIEVINALRE
CCCHHHHHHHHHHHCCHHHHHHHHHHHEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHH
AGVHWDKVEQVASEGLPLDGQTWVITGALDSMARDEAKAKLQALGAKVSGSISAKTTALL
CCCCHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHCCHHCCCCCCCEEEEE
AGDKAGSKMAKAEKLGVKVVGEEEFLVLVGE
ECCCCCHHHHHHHHCCEEEECCCEEEEEECC
>Mature Secondary Structure 
TDPIAPLTSKDINTAKSAANEDAIVAQMRTFIDALKKHNYAYYVLDNPILEDSEYDQLR
CCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHH
RSLLELEEEYPDLIQPDSPINQVGDMPLSAFTQVTHDIPMLSLGNVFEYDDLRDFMRRVN
HHHHHHHHHCCCCCCCCCCHHHHCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHH
DRLSVAQQSPEYEMELKLDGLAVSLKYAYGKFVQAVTRGDGQTGEDITQNAKTIRNLPLW
HHHHHHHCCCCCEEEEEECCEEEEEHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCEE
IPAASDIELLEVRGEVLMPKAGFERLNRLAEEKGDKTFANPRNAAAGSLRQLDPAIAASR
ECCCCCCCEEEECCCEECCCHHHHHHHHHHHHCCCCCCCCCCCHHHCCHHHCCHHHHCCC
PLAFYCYSVNQGLPEHIKTQSAALAWLKTIGFTVSAVEVVQNPREAQAYYESVKETRGDL
CEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHCCCC
PFEIDGMVIKVNSLALQQQLGFLSREPRWATAYKFPAETVMTRLHAIEWQVGRTGAITPV
CEEECCEEEEEHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHEECCCCCCCCCC
GKLEPVKVGGVTVSNVTLHNFGEIQRLDVRAGDMVSVHRAGDVIPKVTRVWTDQRPENSE
CCCCEEEECCEEEECEEECCCCCEEEEEECCCCEEEEHHHCCHHHHHHHHHCCCCCCCCC
PVKLPSTCPVCDSPVVLPKDEALARCTGGLFCPAQQVEALIHFVSRRAMDIDGLGASWLI
CEECCCCCCCCCCCEECCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHH
SFFEHGLVKTVADIYQLHNHQEELITLEKLGEKSVQNILSAIEASKQTTLARFIYALGIR
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GVGETTAQNLAQQFGDLDALMSASIEKLLLTPDVGAITAELTYKFFRAPHNIEVINALRE
CCCHHHHHHHHHHHCCHHHHHHHHHHHEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHH
AGVHWDKVEQVASEGLPLDGQTWVITGALDSMARDEAKAKLQALGAKVSGSISAKTTALL
CCCCHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHCCHHCCCCCCCEEEEE
AGDKAGSKMAKAEKLGVKVVGEEEFLVLVGE
ECCCCCHHHHHHHHCCEEEECCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA