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The map label for this gene is potB [H]

Identifier: 93005069

GI number: 93005069

Start: 280254

End: 281132

Strand: Reverse

Name: potB [H]

Synonym: Pcryo_0238

Alternate gene names: 93005069

Gene position: 281132-280254 (Counterclockwise)

Preceding gene: 93005070

Following gene: 93005068

Centisome position: 9.19

GC content: 45.73

Gene sequence:

>879_bases
ATGGCACATACTGGCATCAGCAATAAAAGCCCTTTTCGTACGGCAACGCTATGGCTGATTTGGGGTTGGCTTCTTATCTT
TGCGCTATTACCCAATATATTGGTCATTGCCGTCAGTTTTCTGACCCGTGATAGCACAGCTTTTATTAGTTTGCCGGTCA
CTATCGACAGCTACGTGCGCATGATTGACCCGCTTTATTTTGATGTCTTTGTGCATTCATTATGGATGGCGGGCATCACT
ACGGTCATCTGCTTATTACTCGGCTACCCCTTTGCTTGGTTTGTATCTCGAGTCAGTAAACGCTGGCAGCCACTGTTGAT
GCTCATGCTAATATTGCCATTTTGGACCAACTCTTTGGTACGAACTTATGCGCTAAAACTCTTATTTGCTAGTAACGGTT
TGATTAACAAATCCTTATTAGCAATCGGCATCATTGATGCGCCCATTGATATTTTGTATACCCAAGGCGCAGTGATTGCT
GGTTTAACGTATTTATTGTTTCCGTTTATGGTGCTGCCGCTATATGCCGTGTTTAGCGATTTGCGCAATGATATGCTGCT
TGCCTCGCAGGATTTGGGCGCATCGAAACCGCAAACCTTTTGGCATGTGGTACTACCCTTAACCACACCTGGTATTATCT
CAGGGGTGCTGTTGGTACTATTGCCAGCAATGGGTATGTTTTATGTTGCCGATATTTTGGGCGGTTCACGCAACTTACTC
GTTGGTAATATTATCAAAAACCAGTTTTTAGATGCCCGTGATTGGCCATTTGGCGCGGCCGCCAGTGTGCTATTGACGCT
TGCTATGGCGGTATTATTGCTTGCCTATCGCGCTAGTAGTCGCCGTATCGGCAAGACTGACTTTAGCGAGGTGGCCTAA

Upstream 100 bases:

>100_bases
ATGAAGATGACCCAAGTTTTGACTATAAGCTTAATGAAGGTGTAAAAGTGTCGTGGGTCGATGGTTGGGAATGGGTATTG
CCAGAAGAGGATATCCACTA

Downstream 100 bases:

>100_bases
TGTCTGATAGTTCACTAAATGGCTCAACAAATAACAAATCGCCGAAAAACAAAAATCCCATAAGGTTTGGCAATATCGTC
GCCAAAGGCTACCTTGGGCT

Product: binding-protein-dependent transport systems inner membrane component

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 292; Mature: 291

Protein sequence:

>292_residues
MAHTGISNKSPFRTATLWLIWGWLLIFALLPNILVIAVSFLTRDSTAFISLPVTIDSYVRMIDPLYFDVFVHSLWMAGIT
TVICLLLGYPFAWFVSRVSKRWQPLLMLMLILPFWTNSLVRTYALKLLFASNGLINKSLLAIGIIDAPIDILYTQGAVIA
GLTYLLFPFMVLPLYAVFSDLRNDMLLASQDLGASKPQTFWHVVLPLTTPGIISGVLLVLLPAMGMFYVADILGGSRNLL
VGNIIKNQFLDARDWPFGAAASVLLTLAMAVLLLAYRASSRRIGKTDFSEVA

Sequences:

>Translated_292_residues
MAHTGISNKSPFRTATLWLIWGWLLIFALLPNILVIAVSFLTRDSTAFISLPVTIDSYVRMIDPLYFDVFVHSLWMAGIT
TVICLLLGYPFAWFVSRVSKRWQPLLMLMLILPFWTNSLVRTYALKLLFASNGLINKSLLAIGIIDAPIDILYTQGAVIA
GLTYLLFPFMVLPLYAVFSDLRNDMLLASQDLGASKPQTFWHVVLPLTTPGIISGVLLVLLPAMGMFYVADILGGSRNLL
VGNIIKNQFLDARDWPFGAAASVLLTLAMAVLLLAYRASSRRIGKTDFSEVA
>Mature_291_residues
AHTGISNKSPFRTATLWLIWGWLLIFALLPNILVIAVSFLTRDSTAFISLPVTIDSYVRMIDPLYFDVFVHSLWMAGITT
VICLLLGYPFAWFVSRVSKRWQPLLMLMLILPFWTNSLVRTYALKLLFASNGLINKSLLAIGIIDAPIDILYTQGAVIAG
LTYLLFPFMVLPLYAVFSDLRNDMLLASQDLGASKPQTFWHVVLPLTTPGIISGVLLVLLPAMGMFYVADILGGSRNLLV
GNIIKNQFLDARDWPFGAAASVLLTLAMAVLLLAYRASSRRIGKTDFSEVA

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787369, Length=266, Percent_Identity=54.8872180451128, Blast_Score=315, Evalue=2e-87,
Organism=Escherichia coli, GI1787080, Length=220, Percent_Identity=39.5454545454545, Blast_Score=179, Evalue=1e-46,
Organism=Escherichia coli, GI1787713, Length=258, Percent_Identity=29.0697674418605, Blast_Score=101, Evalue=6e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 32377; Mature: 32246

Theoretical pI: Translated: 9.71; Mature: 9.71

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAHTGISNKSPFRTATLWLIWGWLLIFALLPNILVIAVSFLTRDSTAFISLPVTIDSYVR
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHH
MIDPLYFDVFVHSLWMAGITTVICLLLGYPFAWFVSRVSKRWQPLLMLMLILPFWTNSLV
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RTYALKLLFASNGLINKSLLAIGIIDAPIDILYTQGAVIAGLTYLLFPFMVLPLYAVFSD
HHHHHHHHHHCCCCCHHHHHHHEEHHCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
LRNDMLLASQDLGASKPQTFWHVVLPLTTPGIISGVLLVLLPAMGMFYVADILGGSRNLL
HHHHHHHHCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
VGNIIKNQFLDARDWPFGAAASVLLTLAMAVLLLAYRASSRRIGKTDFSEVA
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCC
>Mature Secondary Structure 
AHTGISNKSPFRTATLWLIWGWLLIFALLPNILVIAVSFLTRDSTAFISLPVTIDSYVR
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHH
MIDPLYFDVFVHSLWMAGITTVICLLLGYPFAWFVSRVSKRWQPLLMLMLILPFWTNSLV
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RTYALKLLFASNGLINKSLLAIGIIDAPIDILYTQGAVIAGLTYLLFPFMVLPLYAVFSD
HHHHHHHHHHCCCCCHHHHHHHEEHHCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
LRNDMLLASQDLGASKPQTFWHVVLPLTTPGIISGVLLVLLPAMGMFYVADILGGSRNLL
HHHHHHHHCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
VGNIIKNQFLDARDWPFGAAASVLLTLAMAVLLLAYRASSRRIGKTDFSEVA
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]