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The map label for this gene is dapF [H]

Identifier: 93005051

GI number: 93005051

Start: 263966

End: 264853

Strand: Direct

Name: dapF [H]

Synonym: Pcryo_0220

Alternate gene names: 93005051

Gene position: 263966-264853 (Clockwise)

Preceding gene: 93005050

Following gene: 93005052

Centisome position: 8.63

GC content: 48.09

Gene sequence:

>888_bases
ATGCTAATAGAATTTACTAAGATGCATGGGTTGGGCAATGACTTTATGGTCATCGATTTGGTGACTCAGCGTTTGGACTT
GACCAAGGATTTGGTGCAGTTACTGGGTGATCGTCATTTAGGTATTGGTTTTGATCAATTGCTCGTCGTTGAGCCGCCGA
TGCGCCCCGACGTTGATTTTAGTTATCGTATTTTTAATACGGATGGCACAGAAGTTGAGCAATGCGGTAATGGCGCCCGC
TGTTTTGCCCGTTTTGTGCAAGCGCGCAAGTTATCCTTTAAACAACGTCTCCGTGTTGAGACGGCAAGCGGCATTATTTC
GCTCACGACCGATCGTTATGGCTGGGTAGAGGTTGATATGGGCAAGCCCAAGTTTGAGCCAAGTGAGATTCCATTTACCC
CAAGAGCCATCACCAAAATCCAAAATGCTTATCATCTGGACGTGAATGGTACGCCGGTGCAGCTTTATGTTGCCAATATG
GGCAATCCGCATGCGGTGATAAAAGTTGACGATGTGCTTGATGCTGACGTTGAAACCTTGGGTAAAGCCATCGAATCGCA
TCCAGCGTTTCCAGAACGTGTCAATGTCGGTTTTATGCAAATAATGAATCAACGTCATATTCGTCTAAGAGTATTCGAAC
GTGGTGTCGGTGAGACGCAAGCCTGTGGTACGGGCGCTTGTGCAGCCGTTGCCATCGGTATACGCGAAGGTTGGCTTGAC
GAAGGTGAAGATGTGCGTGCTCAGCTCTATGGCGGCAGCATGGTAATTAAATGGCAGCCGGGCTATTCGGTCATGATGAC
CGGACCAACCGCATTTGTGTATGAAGGTGTCTTTAGTCCAGATGGCTTGATGGCACAAGCCGGTATCAAGCCCAATCCAG
AAAGTTAA

Upstream 100 bases:

>100_bases
TGGAATATAACTTGGCATTTGATAACGGTGATAGTTATGATTGTAACTGACAGCGTGCTAATATAAGACATACATAAAAA
TAGGATAGGATATCAGGGAT

Downstream 100 bases:

>100_bases
TAGTAAAACATAAGTGACAGTTATTTTTACTGGATAAGTAATGGGGTTGTTTTCAATATGGTGATGTCTTATAAAAAATC
TCAAGTGATAGACGCACGAT

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase [H]

Number of amino acids: Translated: 295; Mature: 295

Protein sequence:

>295_residues
MLIEFTKMHGLGNDFMVIDLVTQRLDLTKDLVQLLGDRHLGIGFDQLLVVEPPMRPDVDFSYRIFNTDGTEVEQCGNGAR
CFARFVQARKLSFKQRLRVETASGIISLTTDRYGWVEVDMGKPKFEPSEIPFTPRAITKIQNAYHLDVNGTPVQLYVANM
GNPHAVIKVDDVLDADVETLGKAIESHPAFPERVNVGFMQIMNQRHIRLRVFERGVGETQACGTGACAAVAIGIREGWLD
EGEDVRAQLYGGSMVIKWQPGYSVMMTGPTAFVYEGVFSPDGLMAQAGIKPNPES

Sequences:

>Translated_295_residues
MLIEFTKMHGLGNDFMVIDLVTQRLDLTKDLVQLLGDRHLGIGFDQLLVVEPPMRPDVDFSYRIFNTDGTEVEQCGNGAR
CFARFVQARKLSFKQRLRVETASGIISLTTDRYGWVEVDMGKPKFEPSEIPFTPRAITKIQNAYHLDVNGTPVQLYVANM
GNPHAVIKVDDVLDADVETLGKAIESHPAFPERVNVGFMQIMNQRHIRLRVFERGVGETQACGTGACAAVAIGIREGWLD
EGEDVRAQLYGGSMVIKWQPGYSVMMTGPTAFVYEGVFSPDGLMAQAGIKPNPES
>Mature_295_residues
MLIEFTKMHGLGNDFMVIDLVTQRLDLTKDLVQLLGDRHLGIGFDQLLVVEPPMRPDVDFSYRIFNTDGTEVEQCGNGAR
CFARFVQARKLSFKQRLRVETASGIISLTTDRYGWVEVDMGKPKFEPSEIPFTPRAITKIQNAYHLDVNGTPVQLYVANM
GNPHAVIKVDDVLDADVETLGKAIESHPAFPERVNVGFMQIMNQRHIRLRVFERGVGETQACGTGACAAVAIGIREGWLD
EGEDVRAQLYGGSMVIKWQPGYSVMMTGPTAFVYEGVFSPDGLMAQAGIKPNPES

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family [H]

Homologues:

Organism=Escherichia coli, GI87082334, Length=275, Percent_Identity=57.0909090909091, Blast_Score=318, Evalue=3e-88,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001653
- InterPro:   IPR018510 [H]

Pfam domain/function: PF01678 DAP_epimerase [H]

EC number: =5.1.1.7 [H]

Molecular weight: Translated: 32711; Mature: 32711

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIEFTKMHGLGNDFMVIDLVTQRLDLTKDLVQLLGDRHLGIGFDQLLVVEPPMRPDVDF
CEEEEEECCCCCCCEEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCE
SYRIFNTDGTEVEQCGNGARCFARFVQARKLSFKQRLRVETASGIISLTTDRYGWVEVDM
EEEEECCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHEEECCCCEEEEECCCCCEEEEEC
GKPKFEPSEIPFTPRAITKIQNAYHLDVNGTPVQLYVANMGNPHAVIKVDDVLDADVETL
CCCCCCCCCCCCCCHHHHHHCCEEEECCCCCEEEEEEECCCCCEEEEEECCHHCCCHHHH
GKAIESHPAFPERVNVGFMQIMNQRHIRLRVFERGVGETQACGTGACAAVAIGIREGWLD
HHHHHHCCCCCHHCCHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCHHHEEEEHHHCCCC
EGEDVRAQLYGGSMVIKWQPGYSVMMTGPTAFVYEGVFSPDGLMAQAGIKPNPES
CCCCEEEEEECCEEEEEECCCCEEEEECCCEEEEECCCCCCCCEEECCCCCCCCC
>Mature Secondary Structure
MLIEFTKMHGLGNDFMVIDLVTQRLDLTKDLVQLLGDRHLGIGFDQLLVVEPPMRPDVDF
CEEEEEECCCCCCCEEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCE
SYRIFNTDGTEVEQCGNGARCFARFVQARKLSFKQRLRVETASGIISLTTDRYGWVEVDM
EEEEECCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHEEECCCCEEEEECCCCCEEEEEC
GKPKFEPSEIPFTPRAITKIQNAYHLDVNGTPVQLYVANMGNPHAVIKVDDVLDADVETL
CCCCCCCCCCCCCCHHHHHHCCEEEECCCCCEEEEEEECCCCCEEEEEECCHHCCCHHHH
GKAIESHPAFPERVNVGFMQIMNQRHIRLRVFERGVGETQACGTGACAAVAIGIREGWLD
HHHHHHCCCCCHHCCHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCHHHEEEEHHHCCCC
EGEDVRAQLYGGSMVIKWQPGYSVMMTGPTAFVYEGVFSPDGLMAQAGIKPNPES
CCCCEEEEEECCEEEEEECCCCEEEEECCCEEEEECCCCCCCCEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA