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The map label for this gene is tilS [H]

Identifier: 93005006

GI number: 93005006

Start: 205821

End: 207428

Strand: Direct

Name: tilS [H]

Synonym: Pcryo_0175

Alternate gene names: 93005006

Gene position: 205821-207428 (Clockwise)

Preceding gene: 93005005

Following gene: 93005008

Centisome position: 6.73

GC content: 45.27

Gene sequence:

>1608_bases
ATGAGCAGCAGTGCAATCCTACCACATCCTATTGAGCCAATAGCTGAACTGCCGATTGACGATAATTTGGCAAAGGCATT
ATTGACCAGTATGGCTGAGTATGGCGAGCAGCTACAGGGTCGGCGAATTTGGCTGGCCTGTAGTGGTGGGCGTGATTCAT
TGGCACTGGCAGCGCTATGTGTGCAACTCTATCAGCAAGGTCGACTGCCATTTTTGCCGCAACTATTGCATGTCAACCAT
GGCTTGCAAGCGGATAGCGCTATTTGGGCTGCGCATGTTGCTAACTGGGCAAAGGCTCAAAAGATACCATGCACTATTTT
GCAGGCACACGTAAATGGACATGATGAGCAAGCCGCACGCCAAGCACGTTATGATGTGATGCGTGCGCAACTCAATCAAG
ATGATGTATTACTATTAGCCCATCATGCTGATGACCAAGCTGAAACTGTGTTGATGCGGTTGATTCAAGGCGCAGGAGTA
AATGGTCTGTCGGGTATGCATCCGTGGCGTGTACAAACGCAGGACGCGCATCGCATCATAGTATGGCGACCTTGGCTCAT
TGTACAGCGCGCCAGTATTAGCTCATATGCTAAGCGCCTAAAGCTGCCTTATATCGATGATCCCACCAATGACGGCGGTG
ATAACGTCCGTAGTGGACTGCGCCGTGACATCATGCCAGTATTGGCAAAATACAATCCTAACGTTATTGATAACATAGCG
CGTAGCGCGCAGCTACTAAGCGATGCGCAATTAACGGTTAAGGCACAAGCGGCAGAGGATTTAAAGCAGACAGAGGTAAT
ATCATTACAGCTACCATCTGCCCAGCGTGTGCTAAATATAGATGAACTACAAAAACTACCACTTTATCGCCAACGACAAC
TATTGCATTATTGGCTTGGTCAAGATGAGCCATTACCTCCTGCTAAGCAGCTTGTCGATGATGTATTGAGCTTAAGCCAG
CGTCATGATCATGATCATCAAACGGAACTGTTTTGGCAAGGACGCAAACAGTCATATACTATTCGCCGTTATCGTCAGCA
GCTTTATCGTCTAAGTAGCGACTGGCTCAAATGGTTAGAGTTGCCGCTTGCCGAACAGACGCAGAGCTTGTTTACTCATA
CTGAGTCTAATCTTATCGGGGCAGATATTCATACAACTGGAAGACAACCCGTTCCAATTAATCTACGCAATGATGATAAA
TTCACTTGGCAATTACAGATTCTGCCAAATGAAGTAATGCCACTATTAGAAAGCTATAAAGTAGAAAGCGATAAAGATAA
GCTTAGGATTACCTTTGCGCCATTAGATCGCAATCAGCGTGTGCAAACCGCATTGGCGTCGCGTCCACAATCGGGCAAAA
AGCTCTATCAAACCTTAGGTATTCCATCATGGATGCGTGAGAGTTTGGTAGTCGTTAGCGTGACTTTGATAAATAAAGAA
AGTAGTGAAAACGAGCTTTTAGAGAACGATTGCCCGATAGAACAACCTATTTTATTATTATCACCTCTTGAAAACTGGGT
GTTAGCTTCTGATAAGTTGTTAGAAAGCAATCCTCATTATATAGAAAGTGCTATTAGCAGCTGTTTAACCGTACCTAGCT
CGTTTTGA

Upstream 100 bases:

>100_bases
TAGAGGCTTACGCTTAATGTTAAAATAAAAACGTGTCATGTTATGGCGCGTTTTTTTTTGTCAATTAATTCATATACTAA
CTAATGAGATAAGGTCAATG

Downstream 100 bases:

>100_bases
TGTTTGTTACTCAAAGCCTTGAGTAGGGTAGATCTTCATTTTTTTTGATCATTTTGATATCTACTATTTGTATATCTTTA
TAGTGCTCTTCACTCTTTTC

Product: tRNA(Ile)-lysidine synthetase-like

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 535; Mature: 534

Protein sequence:

>535_residues
MSSSAILPHPIEPIAELPIDDNLAKALLTSMAEYGEQLQGRRIWLACSGGRDSLALAALCVQLYQQGRLPFLPQLLHVNH
GLQADSAIWAAHVANWAKAQKIPCTILQAHVNGHDEQAARQARYDVMRAQLNQDDVLLLAHHADDQAETVLMRLIQGAGV
NGLSGMHPWRVQTQDAHRIIVWRPWLIVQRASISSYAKRLKLPYIDDPTNDGGDNVRSGLRRDIMPVLAKYNPNVIDNIA
RSAQLLSDAQLTVKAQAAEDLKQTEVISLQLPSAQRVLNIDELQKLPLYRQRQLLHYWLGQDEPLPPAKQLVDDVLSLSQ
RHDHDHQTELFWQGRKQSYTIRRYRQQLYRLSSDWLKWLELPLAEQTQSLFTHTESNLIGADIHTTGRQPVPINLRNDDK
FTWQLQILPNEVMPLLESYKVESDKDKLRITFAPLDRNQRVQTALASRPQSGKKLYQTLGIPSWMRESLVVVSVTLINKE
SSENELLENDCPIEQPILLLSPLENWVLASDKLLESNPHYIESAISSCLTVPSSF

Sequences:

>Translated_535_residues
MSSSAILPHPIEPIAELPIDDNLAKALLTSMAEYGEQLQGRRIWLACSGGRDSLALAALCVQLYQQGRLPFLPQLLHVNH
GLQADSAIWAAHVANWAKAQKIPCTILQAHVNGHDEQAARQARYDVMRAQLNQDDVLLLAHHADDQAETVLMRLIQGAGV
NGLSGMHPWRVQTQDAHRIIVWRPWLIVQRASISSYAKRLKLPYIDDPTNDGGDNVRSGLRRDIMPVLAKYNPNVIDNIA
RSAQLLSDAQLTVKAQAAEDLKQTEVISLQLPSAQRVLNIDELQKLPLYRQRQLLHYWLGQDEPLPPAKQLVDDVLSLSQ
RHDHDHQTELFWQGRKQSYTIRRYRQQLYRLSSDWLKWLELPLAEQTQSLFTHTESNLIGADIHTTGRQPVPINLRNDDK
FTWQLQILPNEVMPLLESYKVESDKDKLRITFAPLDRNQRVQTALASRPQSGKKLYQTLGIPSWMRESLVVVSVTLINKE
SSENELLENDCPIEQPILLLSPLENWVLASDKLLESNPHYIESAISSCLTVPSSF
>Mature_534_residues
SSSAILPHPIEPIAELPIDDNLAKALLTSMAEYGEQLQGRRIWLACSGGRDSLALAALCVQLYQQGRLPFLPQLLHVNHG
LQADSAIWAAHVANWAKAQKIPCTILQAHVNGHDEQAARQARYDVMRAQLNQDDVLLLAHHADDQAETVLMRLIQGAGVN
GLSGMHPWRVQTQDAHRIIVWRPWLIVQRASISSYAKRLKLPYIDDPTNDGGDNVRSGLRRDIMPVLAKYNPNVIDNIAR
SAQLLSDAQLTVKAQAAEDLKQTEVISLQLPSAQRVLNIDELQKLPLYRQRQLLHYWLGQDEPLPPAKQLVDDVLSLSQR
HDHDHQTELFWQGRKQSYTIRRYRQQLYRLSSDWLKWLELPLAEQTQSLFTHTESNLIGADIHTTGRQPVPINLRNDDKF
TWQLQILPNEVMPLLESYKVESDKDKLRITFAPLDRNQRVQTALASRPQSGKKLYQTLGIPSWMRESLVVVSVTLINKES
SENELLENDCPIEQPILLLSPLENWVLASDKLLESNPHYIESAISSCLTVPSSF

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=342, Percent_Identity=29.5321637426901, Blast_Score=107, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR015262
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3; PF09179 DUF1946; PF11734 TilS_C [H]

EC number: NA

Molecular weight: Translated: 60648; Mature: 60517

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSSAILPHPIEPIAELPIDDNLAKALLTSMAEYGEQLQGRRIWLACSGGRDSLALAALC
CCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHH
VQLYQQGRLPFLPQLLHVNHGLQADSAIWAAHVANWAKAQKIPCTILQAHVNGHDEQAAR
HHHHHCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHH
QARYDVMRAQLNQDDVLLLAHHADDQAETVLMRLIQGAGVNGLSGMHPWRVQTQDAHRII
HHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCCEEE
VWRPWLIVQRASISSYAKRLKLPYIDDPTNDGGDNVRSGLRRDIMPVLAKYNPNVIDNIA
EECCHHEEEHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHH
RSAQLLSDAQLTVKAQAAEDLKQTEVISLQLPSAQRVLNIDELQKLPLYRQRQLLHYWLG
HHHHHHCCCCEEEEHHHHHHHHHHEEEEEECCCHHHHCCHHHHHHCCHHHHHHHHHHHCC
QDEPLPPAKQLVDDVLSLSQRHDHDHQTELFWQGRKQSYTIRRYRQQLYRLSSDWLKWLE
CCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LPLAEQTQSLFTHTESNLIGADIHTTGRQPVPINLRNDDKFTWQLQILPNEVMPLLESYK
CCHHHHHHHHHHHCCCCEEECCEECCCCCCCEEEECCCCCEEEEEEECCHHHHHHHHHHC
VESDKDKLRITFAPLDRNQRVQTALASRPQSGKKLYQTLGIPSWMRESLVVVSVTLINKE
CCCCCCEEEEEEECCCCCHHHHHHHHCCCCHHHHHHHHHCCCHHHHCCEEEEEEEEECCC
SSENELLENDCPIEQPILLLSPLENWVLASDKLLESNPHYIESAISSCLTVPSSF
CCCCHHHHCCCCCCCCEEEECCHHHHHCCCCHHHCCCCHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SSSAILPHPIEPIAELPIDDNLAKALLTSMAEYGEQLQGRRIWLACSGGRDSLALAALC
CCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHH
VQLYQQGRLPFLPQLLHVNHGLQADSAIWAAHVANWAKAQKIPCTILQAHVNGHDEQAAR
HHHHHCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHH
QARYDVMRAQLNQDDVLLLAHHADDQAETVLMRLIQGAGVNGLSGMHPWRVQTQDAHRII
HHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCCEEE
VWRPWLIVQRASISSYAKRLKLPYIDDPTNDGGDNVRSGLRRDIMPVLAKYNPNVIDNIA
EECCHHEEEHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHH
RSAQLLSDAQLTVKAQAAEDLKQTEVISLQLPSAQRVLNIDELQKLPLYRQRQLLHYWLG
HHHHHHCCCCEEEEHHHHHHHHHHEEEEEECCCHHHHCCHHHHHHCCHHHHHHHHHHHCC
QDEPLPPAKQLVDDVLSLSQRHDHDHQTELFWQGRKQSYTIRRYRQQLYRLSSDWLKWLE
CCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LPLAEQTQSLFTHTESNLIGADIHTTGRQPVPINLRNDDKFTWQLQILPNEVMPLLESYK
CCHHHHHHHHHHHCCCCEEECCEECCCCCCCEEEECCCCCEEEEEEECCHHHHHHHHHHC
VESDKDKLRITFAPLDRNQRVQTALASRPQSGKKLYQTLGIPSWMRESLVVVSVTLINKE
CCCCCCEEEEEEECCCCCHHHHHHHHCCCCHHHHHHHHHCCCHHHHCCEEEEEEEEECCC
SSENELLENDCPIEQPILLLSPLENWVLASDKLLESNPHYIESAISSCLTVPSSF
CCCCHHHHCCCCCCCCEEEECCHHHHHCCCCHHHCCCCHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA