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The map label for this gene is hemL [H]

Identifier: 93005002

GI number: 93005002

Start: 200923

End: 202224

Strand: Direct

Name: hemL [H]

Synonym: Pcryo_0171

Alternate gene names: 93005002

Gene position: 200923-202224 (Clockwise)

Preceding gene: 93005001

Following gene: 93005004

Centisome position: 6.57

GC content: 46.16

Gene sequence:

>1302_bases
ATGAGCACTAAAAATGAACAACTATTCGCGCAAGCACGTAAGCATATCCCTGGCGGTGTAAATTCACCTGTCCGAGCTTT
TGCTGGTGTCGGTGGTACGCCAATCTTTATGCACCGTGCCAATGGTAGCAAAATCTATGATACCGAAGACAATGCTTATA
TTGATTATGTTGGCTCTTGGGGTCCAATGATTTTGGGTCATGCCCATCCAAAAGTTATTGATGCGGTCAAAAAAGCGGCT
GATGATGGTTTGAGCTTTGGTACGCCAACGCCATTTGAGACCACCGTTGCCGATAAAATTTGCGAAATCGTTCCGAGTGT
TGAGATGATTCGTATGACCAGCTCAGGCACGGAAGCGACGATGAGTGCGATTCGTTTGGCACGTGGTTATACTCAGCGTG
ACAAAATCGTCAAGTTTGAAGGCTGCTACCATGGTCATTCAGACAGCCTATTAGTAAAAGCAGGCTCAGGCATGCTGGAT
ATTGGTGAGCCTACCTCAAAAGGTGTACCAGCAGATTTTGCCAAGCATACTATTACGATTCCTTATAACGATCCACAAGC
GATTAAAGACTGTTTCGAAAAGTGGGGCGAAGAGATTGCCTGCGTTATCTTAGAGCCTATCGCGGGCAACATGAACATGG
TGATTCCAAGCCAAGAATTCCATGATACGTTGCGTGCAGAGTGTACTGCCAATGGCGCGGTATTAATCTTTGATGAAGTC
ATGACGGGCTTTCGCGTTGGACTTGGCGGTGCGCAAGCGCACTTCGGTATCGACCCTGATTTAACTTGCTTTGGCAAAAT
CATCGGTGCAGGTCTGCCAGTTGGTGCGTTCGGCGGTAAGAAAGACATCATGTCTTGCATTGCACCACTTGGTGGTGTCT
ATCAAGCGGGTACGTTATCCGGTAATCCGCTGGCGATGCGCGCTGGTATCGCCATGTTTGAGGATTTAACTGCTGAAGGC
TTCTATGATGAGCTATCAGCGAAAGTAGACCATTTGGTTGATGGCTTCCAAGCGGCTGCTGACAAGCATGGCATCAATAT
GCGCACCAATAAATTGGGCGGTATGTTTGGAATGTTCTTTGTCAAAGACAGTGCGACGACTGTACCTCAAAACTTTGATG
ACGTGACTGAGTGTGATATGGAGTTATTTAATACTTTCTTCCACGGTATGCTTGATCGTGGTATTTATCTGGCACCGTCT
GCTTATGAAGCAGGCTTTATGTCTATCAAGCATAGTAACGAAGATATTGAAGCGACTATTAAAGCCGCTGATGAAATATT
TGCAGAAATGGCAAAAGCGTAA

Upstream 100 bases:

>100_bases
AAGCTATACATTTATTGTGAACAAAATAATACAAATTACTGAATAAATAATCCCTAAGTATAAAATCACTAAATAAAAAA
CGATTAAGTAGAGAATAACT

Downstream 100 bases:

>100_bases
CACAGTAGTTTTAGGCGTCTAAAAAAACCAGCATAGTGACAAGATTACTGTGCTGGTTTTTTTATGAATAATAAAGGAAG
TTGTATTTATCGCACTTTAT

Product: glutamate-1-semialdehyde aminotransferase

Products: NA

Alternate protein names: GSA; Glutamate-1-semialdehyde aminotransferase; GSA-AT [H]

Number of amino acids: Translated: 433; Mature: 432

Protein sequence:

>433_residues
MSTKNEQLFAQARKHIPGGVNSPVRAFAGVGGTPIFMHRANGSKIYDTEDNAYIDYVGSWGPMILGHAHPKVIDAVKKAA
DDGLSFGTPTPFETTVADKICEIVPSVEMIRMTSSGTEATMSAIRLARGYTQRDKIVKFEGCYHGHSDSLLVKAGSGMLD
IGEPTSKGVPADFAKHTITIPYNDPQAIKDCFEKWGEEIACVILEPIAGNMNMVIPSQEFHDTLRAECTANGAVLIFDEV
MTGFRVGLGGAQAHFGIDPDLTCFGKIIGAGLPVGAFGGKKDIMSCIAPLGGVYQAGTLSGNPLAMRAGIAMFEDLTAEG
FYDELSAKVDHLVDGFQAAADKHGINMRTNKLGGMFGMFFVKDSATTVPQNFDDVTECDMELFNTFFHGMLDRGIYLAPS
AYEAGFMSIKHSNEDIEATIKAADEIFAEMAKA

Sequences:

>Translated_433_residues
MSTKNEQLFAQARKHIPGGVNSPVRAFAGVGGTPIFMHRANGSKIYDTEDNAYIDYVGSWGPMILGHAHPKVIDAVKKAA
DDGLSFGTPTPFETTVADKICEIVPSVEMIRMTSSGTEATMSAIRLARGYTQRDKIVKFEGCYHGHSDSLLVKAGSGMLD
IGEPTSKGVPADFAKHTITIPYNDPQAIKDCFEKWGEEIACVILEPIAGNMNMVIPSQEFHDTLRAECTANGAVLIFDEV
MTGFRVGLGGAQAHFGIDPDLTCFGKIIGAGLPVGAFGGKKDIMSCIAPLGGVYQAGTLSGNPLAMRAGIAMFEDLTAEG
FYDELSAKVDHLVDGFQAAADKHGINMRTNKLGGMFGMFFVKDSATTVPQNFDDVTECDMELFNTFFHGMLDRGIYLAPS
AYEAGFMSIKHSNEDIEATIKAADEIFAEMAKA
>Mature_432_residues
STKNEQLFAQARKHIPGGVNSPVRAFAGVGGTPIFMHRANGSKIYDTEDNAYIDYVGSWGPMILGHAHPKVIDAVKKAAD
DGLSFGTPTPFETTVADKICEIVPSVEMIRMTSSGTEATMSAIRLARGYTQRDKIVKFEGCYHGHSDSLLVKAGSGMLDI
GEPTSKGVPADFAKHTITIPYNDPQAIKDCFEKWGEEIACVILEPIAGNMNMVIPSQEFHDTLRAECTANGAVLIFDEVM
TGFRVGLGGAQAHFGIDPDLTCFGKIIGAGLPVGAFGGKKDIMSCIAPLGGVYQAGTLSGNPLAMRAGIAMFEDLTAEGF
YDELSAKVDHLVDGFQAAADKHGINMRTNKLGGMFGMFFVKDSATTVPQNFDDVTECDMELFNTFFHGMLDRGIYLAPSA
YEAGFMSIKHSNEDIEATIKAADEIFAEMAKA

Specific function: Porphyrin biosynthesis by the C5 pathway; second step. [C]

COG id: COG0001

COG function: function code H; Glutamate-1-semialdehyde aminotransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557809, Length=329, Percent_Identity=29.1793313069909, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI13994255, Length=380, Percent_Identity=25.5263157894737, Blast_Score=96, Evalue=8e-20,
Organism=Homo sapiens, GI24119277, Length=360, Percent_Identity=25.5555555555556, Blast_Score=93, Evalue=5e-19,
Organism=Homo sapiens, GI37574042, Length=362, Percent_Identity=24.585635359116, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI284507298, Length=246, Percent_Identity=30.4878048780488, Blast_Score=79, Evalue=9e-15,
Organism=Homo sapiens, GI226442709, Length=331, Percent_Identity=24.773413897281, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI226442705, Length=360, Percent_Identity=24.1666666666667, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI1786349, Length=428, Percent_Identity=58.6448598130841, Blast_Score=531, Evalue=1e-152,
Organism=Escherichia coli, GI1789016, Length=386, Percent_Identity=29.0155440414508, Blast_Score=142, Evalue=5e-35,
Organism=Escherichia coli, GI1789759, Length=332, Percent_Identity=28.9156626506024, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1788044, Length=350, Percent_Identity=28.2857142857143, Blast_Score=118, Evalue=8e-28,
Organism=Escherichia coli, GI145693181, Length=356, Percent_Identity=27.5280898876405, Blast_Score=114, Evalue=9e-27,
Organism=Escherichia coli, GI1787560, Length=281, Percent_Identity=29.1814946619217, Blast_Score=105, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI71992977, Length=432, Percent_Identity=24.537037037037, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI25144271, Length=317, Percent_Identity=27.1293375394322, Blast_Score=99, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI25144274, Length=161, Percent_Identity=29.1925465838509, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6323470, Length=305, Percent_Identity=30.1639344262295, Blast_Score=112, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6324432, Length=345, Percent_Identity=25.5072463768116, Blast_Score=84, Evalue=4e-17,
Organism=Drosophila melanogaster, GI21357415, Length=299, Percent_Identity=26.4214046822742, Blast_Score=91, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004639
- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00202 Aminotran_3 [H]

EC number: =5.4.3.8 [H]

Molecular weight: Translated: 46487; Mature: 46356

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTKNEQLFAQARKHIPGGVNSPVRAFAGVGGTPIFMHRANGSKIYDTEDNAYIDYVGSW
CCCCHHHHHHHHHHHCCCCCCCHHHHHHCCCCCEEEEECCCCCEEEECCCCEEEEEECCC
GPMILGHAHPKVIDAVKKAADDGLSFGTPTPFETTVADKICEIVPSVEMIRMTSSGTEAT
CCEEECCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCHHHH
MSAIRLARGYTQRDKIVKFEGCYHGHSDSLLVKAGSGMLDIGEPTSKGVPADFAKHTITI
HHHHHHHHCCCCCCCEEEEECCCCCCCCCEEEEECCCEEECCCCCCCCCCCCCCCCEEEE
PYNDPQAIKDCFEKWGEEIACVILEPIAGNMNMVIPSQEFHDTLRAECTANGAVLIFDEV
CCCCHHHHHHHHHHHCCCEEEEEEECCCCCCEEEECCHHHHHHHHHHHCCCCEEEEHHHH
MTGFRVGLGGAQAHFGIDPDLTCFGKIIGAGLPVGAFGGKKDIMSCIAPLGGVYQAGTLS
HHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCEECCCCC
GNPLAMRAGIAMFEDLTAEGFYDELSAKVDHLVDGFQAAADKHGINMRTNKLGGMFGMFF
CCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHCCEEEEEE
VKDSATTVPQNFDDVTECDMELFNTFFHGMLDRGIYLAPSAYEAGFMSIKHSNEDIEATI
EECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCEEEEECCCCCHHHHH
KAADEIFAEMAKA
HHHHHHHHHHHCC
>Mature Secondary Structure 
STKNEQLFAQARKHIPGGVNSPVRAFAGVGGTPIFMHRANGSKIYDTEDNAYIDYVGSW
CCCHHHHHHHHHHHCCCCCCCHHHHHHCCCCCEEEEECCCCCEEEECCCCEEEEEECCC
GPMILGHAHPKVIDAVKKAADDGLSFGTPTPFETTVADKICEIVPSVEMIRMTSSGTEAT
CCEEECCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCHHHH
MSAIRLARGYTQRDKIVKFEGCYHGHSDSLLVKAGSGMLDIGEPTSKGVPADFAKHTITI
HHHHHHHHCCCCCCCEEEEECCCCCCCCCEEEEECCCEEECCCCCCCCCCCCCCCCEEEE
PYNDPQAIKDCFEKWGEEIACVILEPIAGNMNMVIPSQEFHDTLRAECTANGAVLIFDEV
CCCCHHHHHHHHHHHCCCEEEEEEECCCCCCEEEECCHHHHHHHHHHHCCCCEEEEHHHH
MTGFRVGLGGAQAHFGIDPDLTCFGKIIGAGLPVGAFGGKKDIMSCIAPLGGVYQAGTLS
HHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCEECCCCC
GNPLAMRAGIAMFEDLTAEGFYDELSAKVDHLVDGFQAAADKHGINMRTNKLGGMFGMFF
CCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHCCEEEEEE
VKDSATTVPQNFDDVTECDMELFNTFFHGMLDRGIYLAPSAYEAGFMSIKHSNEDIEATI
EECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCEEEEECCCCCHHHHH
KAADEIFAEMAKA
HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA