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The map label for this gene is gph2 [H]

Identifier: 93004962

GI number: 93004962

Start: 154096

End: 154806

Strand: Direct

Name: gph2 [H]

Synonym: Pcryo_0131

Alternate gene names: 93004962

Gene position: 154096-154806 (Clockwise)

Preceding gene: 93004961

Following gene: 93004963

Centisome position: 5.04

GC content: 45.15

Gene sequence:

>711_bases
ATGAGCCAATTTGTAAAAGCGGTCTTATTTGACCTAGATGGGACATTGATAGATACTGCTGCCGATTTTGTACGTATTAT
TGGCAAAATGAGCCAAGAAAACGGCTGGCAAGCGCCGTCTGAGACAGAGATTCGTGAGCAAGTATCGGCAGGTGCTTCAG
CGATGGTGCAGCTTATGCTACGTCATAATGAGCAAACAGACTTTAGCGAAGAGACGTTACTTGAGTTTCGTCAGCAGTTT
TTAGACGATTATGAAGCGGATATTTGTGTGGATAGTCATGTTTTTGACACGCTTGAGGACGTTTTAAGTGCCCTTGAAGA
AAAAGGTGTACCGTGGGGTATAGTGACCAATAAACCGCGCTATTTGTCAGAGCTGTTATTAGAAAAAATGCAGCTAGATA
CGCGCTGTGCGGTTTTGGTTTGTCCTGATGATGTGTCACGCCCTAAACCTGACCCAGAGCCTATGTATGCGGCGCTAGAG
AAGCTTGGTATCCCTCGCGGTGCTGCTGCAAGTGTGATTTATGTTGGCGATCATATCCGTGATATCGAAGCTGGTAATGC
CGCTGGTATGCCGACGATTTTGGCCGCTTATGGTTACATTCCGCCTGAAGATCAAAACAACCTGAAGAAATGGGGCGCAG
ATTATATTACGGAAACCCCTGAGCAGTTGAGTAAACTACTGCTTTCTTCTGGTAAATTCGACTATTTATAA

Upstream 100 bases:

>100_bases
CGTTACTGGCTAGCTCAAAACGTCGATGTCAACTATATGATAGCAGTACAGAAACCTCTCGCTTAATACCCTTTTATCAC
CTTAATTAAGAGCCTTCGTA

Downstream 100 bases:

>100_bases
TTCAAATGTTATAACTCAAATGAGCCACAAGATCAGCGTGTTATCAGGCAATATTTAACAACATAGCATAATGGTTAACC
ATACCATTTATTAAACGATA

Product: HAD family hydrolase

Products: NA

Alternate protein names: PGP 2; PGPase2 [H]

Number of amino acids: Translated: 236; Mature: 235

Protein sequence:

>236_residues
MSQFVKAVLFDLDGTLIDTAADFVRIIGKMSQENGWQAPSETEIREQVSAGASAMVQLMLRHNEQTDFSEETLLEFRQQF
LDDYEADICVDSHVFDTLEDVLSALEEKGVPWGIVTNKPRYLSELLLEKMQLDTRCAVLVCPDDVSRPKPDPEPMYAALE
KLGIPRGAAASVIYVGDHIRDIEAGNAAGMPTILAAYGYIPPEDQNNLKKWGADYITETPEQLSKLLLSSGKFDYL

Sequences:

>Translated_236_residues
MSQFVKAVLFDLDGTLIDTAADFVRIIGKMSQENGWQAPSETEIREQVSAGASAMVQLMLRHNEQTDFSEETLLEFRQQF
LDDYEADICVDSHVFDTLEDVLSALEEKGVPWGIVTNKPRYLSELLLEKMQLDTRCAVLVCPDDVSRPKPDPEPMYAALE
KLGIPRGAAASVIYVGDHIRDIEAGNAAGMPTILAAYGYIPPEDQNNLKKWGADYITETPEQLSKLLLSSGKFDYL
>Mature_235_residues
SQFVKAVLFDLDGTLIDTAADFVRIIGKMSQENGWQAPSETEIREQVSAGASAMVQLMLRHNEQTDFSEETLLEFRQQFL
DDYEADICVDSHVFDTLEDVLSALEEKGVPWGIVTNKPRYLSELLLEKMQLDTRCAVLVCPDDVSRPKPDPEPMYAALEK
LGIPRGAAASVIYVGDHIRDIEAGNAAGMPTILAAYGYIPPEDQNNLKKWGADYITETPEQLSKLLLSSGKFDYL

Specific function: Specifically catalyzes the dephosphorylation of 2- phosphoglycolate. Is involved in the dissimilation of the intracellular 2-phosphoglycolate formed during the DNA repair of 3'-phosphoglycolate ends, a major class of DNA lesions induced by oxidative stres

COG id: COG0546

COG function: function code R; Predicted phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family [H]

Homologues:

Organism=Escherichia coli, GI1789787, Length=217, Percent_Identity=29.4930875576037, Blast_Score=96, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR006346
- InterPro:   IPR023198 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: =3.1.3.18 [H]

Molecular weight: Translated: 26278; Mature: 26147

Theoretical pI: Translated: 4.14; Mature: 4.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQFVKAVLFDLDGTLIDTAADFVRIIGKMSQENGWQAPSETEIREQVSAGASAMVQLML
CHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHH
RHNEQTDFSEETLLEFRQQFLDDYEADICVDSHVFDTLEDVLSALEEKGVPWGIVTNKPR
HHCCCCCCHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHCCCCEEEECCCHH
YLSELLLEKMQLDTRCAVLVCPDDVSRPKPDPEPMYAALEKLGIPRGAAASVIYVGDHIR
HHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHCCCCCCCCEEEEECCCHH
DIEAGNAAGMPTILAAYGYIPPEDQNNLKKWGADYITETPEQLSKLLLSSGKFDYL
HCCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SQFVKAVLFDLDGTLIDTAADFVRIIGKMSQENGWQAPSETEIREQVSAGASAMVQLML
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHH
RHNEQTDFSEETLLEFRQQFLDDYEADICVDSHVFDTLEDVLSALEEKGVPWGIVTNKPR
HHCCCCCCHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHCCCCEEEECCCHH
YLSELLLEKMQLDTRCAVLVCPDDVSRPKPDPEPMYAALEKLGIPRGAAASVIYVGDHIR
HHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHCCCCCCCCEEEEECCCHH
DIEAGNAAGMPTILAAYGYIPPEDQNNLKKWGADYITETPEQLSKLLLSSGKFDYL
HCCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]