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The map label for this gene is pcnB [H]

Identifier: 93004954

GI number: 93004954

Start: 145835

End: 148096

Strand: Direct

Name: pcnB [H]

Synonym: Pcryo_0123

Alternate gene names: 93004954

Gene position: 145835-148096 (Clockwise)

Preceding gene: 93004953

Following gene: 93004955

Centisome position: 4.77

GC content: 44.74

Gene sequence:

>2262_bases
ATGGCCGAGCGTGCCGCCAAGCAGTTAGAACTGAATAAATCTGAATTACCCAATTCTATTACCGAAGTAATCGCAACCCT
AACCCGAGCTGGGTTTGACGCCTATATCGTCGGTGGTGGCGTGCGTGATACCTTGCTAGGACTGCGTCCAAAAGATTTTG
ACGCGGTCACTGATGCCAAGCCTCATGAGATTAAAGACGTCTTTGGTAAGCGCTGCCGTATTATCGGTCGCCGTTTTCAG
TTGGCGCATGTGTACTCTGGGCGTGAGCTTATCGAAGTAGCCACCTTCCGTGGTCCACCTATTAATGATTCTAATACCAA
TCAAGACGGTATGATTCTGCGCGACAATGTATGGGGTGATATCAAACAAGACTTTGCCCGTCGTGATTTTTCTATTAATG
CCCTTTATTATCAGCCGCTCAAAGGCATAGTTCATGATTTTTGCGACGCGCTTGAAGATATTGATAATAAAATTATCCGT
TTATTAGGCAATGCGCCGATTCGTATCGAAGAAGATCCGGTCAGATTGCTGCGTGCCTTGCGTTTCAAAGCAAAATTGGG
TTTTGAGTTTGATAGCGAATTAGCCGCACAGTTCCATGATGGCAACTGGTCATTACTTGAACAAATCTCTCCGCACCGTC
TATATGATGAAACGCAAAAGATGTTTACCGGTGGTTATTTAGTACCATTATTACCGTTATTGTTTGATTCAGGCGCGATT
GATAGTCTTATCATTTATCCGCCTTCTGAGCCAAGTGCCTTAGTTAAACAAGTCGCTATTAATACCGACAAACGTATTGC
TGCTGGCAAAAGTATCAACCCAGCATTCTTTTATGCCGCTTTGCTGTGGGAGAACTACTTACATCAACTAGAAAAAGCCA
AAAAGCGCAATATGCCTTTTGCGGATGCACAAATGCATGCAGCAGGCAAAGTGATTGATCGTCAACGTATCAAGACTGCC
ATTCCAAAATTTGCTGAACAGTTTATTCGTGATATCTGGATATTGCAGCCAAAACTGGCCGCGCCGCGTAGCAAACAGAT
TGTACAGCTATCTGAGCATCCACGTTTTCGTGCCGGTTTTGATTTCTTATTATTGCGCGAGCAGTGCGGCGATGCAGAGC
ATCCACTTTCTGAATCGACTAATGATATGGGCGAGTGGTGGCAGACCTATCAAACCTTGACTGAGCAGCAGCAGGATCAA
GCGATTGATGACTTTGATGAAAATATCCGCCGCGGTGCTTATAAACAAGGTCAGCGTGGTCGTGGACGCAATCGTCAAAA
GCAGCAGCATTCGAATGACAATGCTAAGTTGAGTCAGTTGGATAATGACTATCAAGGTAAAGCGCTGAGTAATGGTCATG
CAAAACCAGCAATGGTTAATGATGCCTCAAATGTGCCATCACGTCGTCGCCGTGCCAGTCAGCATACTTCTGCTAATAGT
AAAAGTTATAGCCCGCAGCAACTCACACAAGACAATAATGAGCTGGCACAGTTGCAGCAATTGTCTTTGGCGAGCAGTAA
TAATGGTAGCAATCAGCAGCACATGCCGAAACCTGCACCATTGTTTGTCATAGAACACAGCAACGTCGTACCGCCTTTGA
AAAAGCAGCTATCTGGTTCAGATAAAGCACTGCCTAAAGCGACGCAAAAAGCGCCTATTCAAAAAGTGCTTGAGCAAGCA
ACAGTTAGTAAAAAGGCATCGGCTGATAGTCAATCGCAGCTGCAAGAAAAAGCTGTTAATAAAGCGAGTGACTTAACTAA
TAGATCGCATACACCAAAAGAGTCAGTGAATACTCAGGTGTCTTCAACTAATGCTCGTAGCTCCGCTTATGCAGAGCGCA
ACGTCGCGCAGTCACCAGCACTTGTTAGTATGAATAATGAGCCGATACCGCACAAGCGCCGTCGTCGTCAGCCTACTGTG
ATTGAAAATAGTGGTATTGTCGCAGAGGCTAGCAGTGCTAAAGAAGCAACGACAATAGACACCAGTAGTAATGAGAAATC
ATCTGGGAAGTACGCAAAAAAAGTGCCTAAACCAGCTGTTCACTCTACTGATAACGTCATTGATAAAGCACCGAAACCCG
CTAAAGCATCAAGTACTAAGTCTACTAAAGCTAAACGAGACGCTAGTGGTCAAAGTGCTACTAATCAAAGTTCTAACGAA
CAGCAAGATGCTAATAAAGTAGCTAACAGTAATATGAATGCCAATAAAGGTCCTGTGCCATCGAAACGTCGCCGTCGTCA
GCCAAGCGTCAATGACGTTTAA

Upstream 100 bases:

>100_bases
ACACCCACCATGGCCAAAATTTGTTAAACTAGCACGTACATCCGCCTGATTGACGCTGTGTTCGTCAGGGTGGTTAAGAA
AAAAGGCAAGGAGTAGATGC

Downstream 100 bases:

>100_bases
CGTTCACAGCGAATATAAGAGTAAAGTGATAACGATGAACACGCGCTTTAATGATACTGATTTTGATAGCAAATCTATCG
CCATTTGCTCTTGGGTCACT

Product: poly(A) polymerase

Products: NA

Alternate protein names: PAP [H]

Number of amino acids: Translated: 753; Mature: 752

Protein sequence:

>753_residues
MAERAAKQLELNKSELPNSITEVIATLTRAGFDAYIVGGGVRDTLLGLRPKDFDAVTDAKPHEIKDVFGKRCRIIGRRFQ
LAHVYSGRELIEVATFRGPPINDSNTNQDGMILRDNVWGDIKQDFARRDFSINALYYQPLKGIVHDFCDALEDIDNKIIR
LLGNAPIRIEEDPVRLLRALRFKAKLGFEFDSELAAQFHDGNWSLLEQISPHRLYDETQKMFTGGYLVPLLPLLFDSGAI
DSLIIYPPSEPSALVKQVAINTDKRIAAGKSINPAFFYAALLWENYLHQLEKAKKRNMPFADAQMHAAGKVIDRQRIKTA
IPKFAEQFIRDIWILQPKLAAPRSKQIVQLSEHPRFRAGFDFLLLREQCGDAEHPLSESTNDMGEWWQTYQTLTEQQQDQ
AIDDFDENIRRGAYKQGQRGRGRNRQKQQHSNDNAKLSQLDNDYQGKALSNGHAKPAMVNDASNVPSRRRRASQHTSANS
KSYSPQQLTQDNNELAQLQQLSLASSNNGSNQQHMPKPAPLFVIEHSNVVPPLKKQLSGSDKALPKATQKAPIQKVLEQA
TVSKKASADSQSQLQEKAVNKASDLTNRSHTPKESVNTQVSSTNARSSAYAERNVAQSPALVSMNNEPIPHKRRRRQPTV
IENSGIVAEASSAKEATTIDTSSNEKSSGKYAKKVPKPAVHSTDNVIDKAPKPAKASSTKSTKAKRDASGQSATNQSSNE
QQDANKVANSNMNANKGPVPSKRRRRQPSVNDV

Sequences:

>Translated_753_residues
MAERAAKQLELNKSELPNSITEVIATLTRAGFDAYIVGGGVRDTLLGLRPKDFDAVTDAKPHEIKDVFGKRCRIIGRRFQ
LAHVYSGRELIEVATFRGPPINDSNTNQDGMILRDNVWGDIKQDFARRDFSINALYYQPLKGIVHDFCDALEDIDNKIIR
LLGNAPIRIEEDPVRLLRALRFKAKLGFEFDSELAAQFHDGNWSLLEQISPHRLYDETQKMFTGGYLVPLLPLLFDSGAI
DSLIIYPPSEPSALVKQVAINTDKRIAAGKSINPAFFYAALLWENYLHQLEKAKKRNMPFADAQMHAAGKVIDRQRIKTA
IPKFAEQFIRDIWILQPKLAAPRSKQIVQLSEHPRFRAGFDFLLLREQCGDAEHPLSESTNDMGEWWQTYQTLTEQQQDQ
AIDDFDENIRRGAYKQGQRGRGRNRQKQQHSNDNAKLSQLDNDYQGKALSNGHAKPAMVNDASNVPSRRRRASQHTSANS
KSYSPQQLTQDNNELAQLQQLSLASSNNGSNQQHMPKPAPLFVIEHSNVVPPLKKQLSGSDKALPKATQKAPIQKVLEQA
TVSKKASADSQSQLQEKAVNKASDLTNRSHTPKESVNTQVSSTNARSSAYAERNVAQSPALVSMNNEPIPHKRRRRQPTV
IENSGIVAEASSAKEATTIDTSSNEKSSGKYAKKVPKPAVHSTDNVIDKAPKPAKASSTKSTKAKRDASGQSATNQSSNE
QQDANKVANSNMNANKGPVPSKRRRRQPSVNDV
>Mature_752_residues
AERAAKQLELNKSELPNSITEVIATLTRAGFDAYIVGGGVRDTLLGLRPKDFDAVTDAKPHEIKDVFGKRCRIIGRRFQL
AHVYSGRELIEVATFRGPPINDSNTNQDGMILRDNVWGDIKQDFARRDFSINALYYQPLKGIVHDFCDALEDIDNKIIRL
LGNAPIRIEEDPVRLLRALRFKAKLGFEFDSELAAQFHDGNWSLLEQISPHRLYDETQKMFTGGYLVPLLPLLFDSGAID
SLIIYPPSEPSALVKQVAINTDKRIAAGKSINPAFFYAALLWENYLHQLEKAKKRNMPFADAQMHAAGKVIDRQRIKTAI
PKFAEQFIRDIWILQPKLAAPRSKQIVQLSEHPRFRAGFDFLLLREQCGDAEHPLSESTNDMGEWWQTYQTLTEQQQDQA
IDDFDENIRRGAYKQGQRGRGRNRQKQQHSNDNAKLSQLDNDYQGKALSNGHAKPAMVNDASNVPSRRRRASQHTSANSK
SYSPQQLTQDNNELAQLQQLSLASSNNGSNQQHMPKPAPLFVIEHSNVVPPLKKQLSGSDKALPKATQKAPIQKVLEQAT
VSKKASADSQSQLQEKAVNKASDLTNRSHTPKESVNTQVSSTNARSSAYAERNVAQSPALVSMNNEPIPHKRRRRQPTVI
ENSGIVAEASSAKEATTIDTSSNEKSSGKYAKKVPKPAVHSTDNVIDKAPKPAKASSTKSTKAKRDASGQSATNQSSNEQ
QDANKVANSNMNANKGPVPSKRRRRQPSVNDV

Specific function: Polymerase that creates the 3' poly(A) tail found in some mRNA's [H]

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=240, Percent_Identity=29.5833333333333, Blast_Score=89, Evalue=1e-17,
Organism=Escherichia coli, GI87081691, Length=418, Percent_Identity=37.799043062201, Blast_Score=276, Evalue=4e-75,
Organism=Escherichia coli, GI1789436, Length=219, Percent_Identity=32.4200913242009, Blast_Score=73, Evalue=8e-14,
Organism=Drosophila melanogaster, GI281360112, Length=222, Percent_Identity=28.8288288288288, Blast_Score=86, Evalue=6e-17,
Organism=Drosophila melanogaster, GI21357337, Length=222, Percent_Identity=28.8288288288288, Blast_Score=86, Evalue=6e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002646
- InterPro:   IPR010206 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.19 [H]

Molecular weight: Translated: 83865; Mature: 83734

Theoretical pI: Translated: 10.17; Mature: 10.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAERAAKQLELNKSELPNSITEVIATLTRAGFDAYIVGGGVRDTLLGLRPKDFDAVTDAK
CCHHHHHHHHCCHHHCCHHHHHHHHHHHHCCCCEEEECCCHHHHHHCCCCCCCCCCCCCC
PHEIKDVFGKRCRIIGRRFQLAHVYSGRELIEVATFRGPPINDSNTNQDGMILRDNVWGD
CHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCH
IKQDFARRDFSINALYYQPLKGIVHDFCDALEDIDNKIIRLLGNAPIRIEEDPVRLLRAL
HHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCHHHHHHHH
RFKAKLGFEFDSELAAQFHDGNWSLLEQISPHRLYDETQKMFTGGYLVPLLPLLFDSGAI
HHHHHCCCCCCHHHHHHHCCCCHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCC
DSLIIYPPSEPSALVKQVAINTDKRIAAGKSINPAFFYAALLWENYLHQLEKAKKRNMPF
CEEEEECCCCHHHHHHHHHHCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
ADAQMHAAGKVIDRQRIKTAIPKFAEQFIRDIWILQPKLAAPRSKQIVQLSEHPRFRAGF
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCHHHHHCCCCCCCCCH
DFLLLREQCGDAEHPLSESTNDMGEWWQTYQTLTEQQQDQAIDDFDENIRRGAYKQGQRG
HHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
RGRNRQKQQHSNDNAKLSQLDNDYQGKALSNGHAKPAMVNDASNVPSRRRRASQHTSANS
CCCCHHHHHHCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCC
KSYSPQQLTQDNNELAQLQQLSLASSNNGSNQQHMPKPAPLFVIEHSNVVPPLKKQLSGS
CCCCHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCCCHHHHHCCCC
DKALPKATQKAPIQKVLEQATVSKKASADSQSQLQEKAVNKASDLTNRSHTPKESVNTQV
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCH
SSTNARSSAYAERNVAQSPALVSMNNEPIPHKRRRRQPTVIENSGIVAEASSAKEATTID
HCCCCHHHHHHHHHHCCCCCEEECCCCCCCHHHHCCCCCEEECCCCEEECCCCCCCEEEC
TSSNEKSSGKYAKKVPKPAVHSTDNVIDKAPKPAKASSTKSTKAKRDASGQSATNQSSNE
CCCCCCCCCCHHHHCCCCCCCCCCHHHHCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCH
QQDANKVANSNMNANKGPVPSKRRRRQPSVNDV
HHHHHHHHCCCCCCCCCCCCCHHHHCCCCCCCC
>Mature Secondary Structure 
AERAAKQLELNKSELPNSITEVIATLTRAGFDAYIVGGGVRDTLLGLRPKDFDAVTDAK
CHHHHHHHHCCHHHCCHHHHHHHHHHHHCCCCEEEECCCHHHHHHCCCCCCCCCCCCCC
PHEIKDVFGKRCRIIGRRFQLAHVYSGRELIEVATFRGPPINDSNTNQDGMILRDNVWGD
CHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCH
IKQDFARRDFSINALYYQPLKGIVHDFCDALEDIDNKIIRLLGNAPIRIEEDPVRLLRAL
HHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCHHHHHHHH
RFKAKLGFEFDSELAAQFHDGNWSLLEQISPHRLYDETQKMFTGGYLVPLLPLLFDSGAI
HHHHHCCCCCCHHHHHHHCCCCHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCC
DSLIIYPPSEPSALVKQVAINTDKRIAAGKSINPAFFYAALLWENYLHQLEKAKKRNMPF
CEEEEECCCCHHHHHHHHHHCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
ADAQMHAAGKVIDRQRIKTAIPKFAEQFIRDIWILQPKLAAPRSKQIVQLSEHPRFRAGF
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCHHHHHCCCCCCCCCH
DFLLLREQCGDAEHPLSESTNDMGEWWQTYQTLTEQQQDQAIDDFDENIRRGAYKQGQRG
HHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
RGRNRQKQQHSNDNAKLSQLDNDYQGKALSNGHAKPAMVNDASNVPSRRRRASQHTSANS
CCCCHHHHHHCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCC
KSYSPQQLTQDNNELAQLQQLSLASSNNGSNQQHMPKPAPLFVIEHSNVVPPLKKQLSGS
CCCCHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCCCHHHHHCCCC
DKALPKATQKAPIQKVLEQATVSKKASADSQSQLQEKAVNKASDLTNRSHTPKESVNTQV
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCH
SSTNARSSAYAERNVAQSPALVSMNNEPIPHKRRRRQPTVIENSGIVAEASSAKEATTID
HCCCCHHHHHHHHHHCCCCCEEECCCCCCCHHHHCCCCCEEECCCCEEECCCCCCCEEEC
TSSNEKSSGKYAKKVPKPAVHSTDNVIDKAPKPAKASSTKSTKAKRDASGQSATNQSSNE
CCCCCCCCCCHHHHCCCCCCCCCCHHHHCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCH
QQDANKVANSNMNANKGPVPSKRRRRQPSVNDV
HHHHHHHHCCCCCCCCCCCCCHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]