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The map label for this gene is folK [H]

Identifier: 93004955

GI number: 93004955

Start: 148131

End: 148697

Strand: Direct

Name: folK [H]

Synonym: Pcryo_0124

Alternate gene names: 93004955

Gene position: 148131-148697 (Clockwise)

Preceding gene: 93004954

Following gene: 93004956

Centisome position: 4.84

GC content: 45.5

Gene sequence:

>567_bases
ATGAACACGCGCTTTAATGATACTGATTTTGATAGCAAATCTATCGCCATTTGCTCTTGGGTCACTTGTTATGTTGGCTT
GGGTAGCAATCTGGTAAATGAGCTTGGGGCGCCTGTTGAGCATTTACAGCACGCGTTTGAGATGATGCAAGAGCAAGAAA
AGATACGCACTGTGCGTATTTCTTCATTTTATGCCTCGGTACCGATGGGACCGCAAGATCAGCCAGATTTTGTCAACGCC
GTTGCTGGCTTTGAAACTATATTTACGCCGTTTGAGCTGCTGACATTTTGCCAACAGTTGGAGCAGCAAGCAAAACGCGC
CCGCCTGCGTCATTGGGGTGAGCGTAGTCTTGATGTGGATATATTGTTATACGGCAATGAGCGGATTTCTGAGCCAACAT
TAACGATACCCCATGTGGGTTTGCATGAGCGTAATTTTGTTTTGATACCGTTACGAGAGCTCGCACCTGAGCTAATGATT
GTAGGAAAGTCGATAATCGACTATCCCCAAAGCAGCGATTGGGCAGGTTTGAAGCTGTTAGAACATGATGCGTCAAGTCA
TAAATAA

Upstream 100 bases:

>100_bases
AATGCCAATAAAGGTCCTGTGCCATCGAAACGTCGCCGTCGTCAGCCAAGCGTCAATGACGTTTAACGTTCACAGCGAAT
ATAAGAGTAAAGTGATAACG

Downstream 100 bases:

>100_bases
AGAGACATTCATTTAAAGTGAATGACAGTTGATTGATATTCAGAAGTATTGAGGATGATATAACGATATGACAACGTTAT
CTACTTTAAATAAGTTTAAA

Product: 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase

Products: NA

Alternate protein names: 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase; PPPK; 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase; HPPK [H]

Number of amino acids: Translated: 188; Mature: 188

Protein sequence:

>188_residues
MNTRFNDTDFDSKSIAICSWVTCYVGLGSNLVNELGAPVEHLQHAFEMMQEQEKIRTVRISSFYASVPMGPQDQPDFVNA
VAGFETIFTPFELLTFCQQLEQQAKRARLRHWGERSLDVDILLYGNERISEPTLTIPHVGLHERNFVLIPLRELAPELMI
VGKSIIDYPQSSDWAGLKLLEHDASSHK

Sequences:

>Translated_188_residues
MNTRFNDTDFDSKSIAICSWVTCYVGLGSNLVNELGAPVEHLQHAFEMMQEQEKIRTVRISSFYASVPMGPQDQPDFVNA
VAGFETIFTPFELLTFCQQLEQQAKRARLRHWGERSLDVDILLYGNERISEPTLTIPHVGLHERNFVLIPLRELAPELMI
VGKSIIDYPQSSDWAGLKLLEHDASSHK
>Mature_188_residues
MNTRFNDTDFDSKSIAICSWVTCYVGLGSNLVNELGAPVEHLQHAFEMMQEQEKIRTVRISSFYASVPMGPQDQPDFVNA
VAGFETIFTPFELLTFCQQLEQQAKRARLRHWGERSLDVDILLYGNERISEPTLTIPHVGLHERNFVLIPLRELAPELMI
VGKSIIDYPQSSDWAGLKLLEHDASSHK

Specific function: Dihydrofolate biosynthesis; first step. [C]

COG id: COG0801

COG function: function code H; 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HPPK family [H]

Homologues:

Organism=Escherichia coli, GI1786335, Length=140, Percent_Identity=42.1428571428571, Blast_Score=120, Evalue=6e-29,
Organism=Saccharomyces cerevisiae, GI6324073, Length=143, Percent_Identity=34.965034965035, Blast_Score=93, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000550 [H]

Pfam domain/function: PF01288 HPPK [H]

EC number: =2.7.6.3 [H]

Molecular weight: Translated: 21389; Mature: 21389

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS00794 HPPK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTRFNDTDFDSKSIAICSWVTCYVGLGSNLVNELGAPVEHLQHAFEMMQEQEKIRTVRI
CCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
SSFYASVPMGPQDQPDFVNAVAGFETIFTPFELLTFCQQLEQQAKRARLRHWGERSLDVD
HHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEE
ILLYGNERISEPTLTIPHVGLHERNFVLIPLRELAPELMIVGKSIIDYPQSSDWAGLKLL
EEEECCCCCCCCCEECCCCCCCCCCEEEEEHHHHCHHHHHHHHHHHCCCCCCCCCCHHHH
EHDASSHK
HCCCCCCC
>Mature Secondary Structure
MNTRFNDTDFDSKSIAICSWVTCYVGLGSNLVNELGAPVEHLQHAFEMMQEQEKIRTVRI
CCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
SSFYASVPMGPQDQPDFVNAVAGFETIFTPFELLTFCQQLEQQAKRARLRHWGERSLDVD
HHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEE
ILLYGNERISEPTLTIPHVGLHERNFVLIPLRELAPELMIVGKSIIDYPQSSDWAGLKLL
EEEECCCCCCCCCEECCCCCCCCCCEEEEEHHHHCHHHHHHHHHHHCCCCCCCCCCHHHH
EHDASSHK
HCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 10080886 [H]