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The map label for this gene is mazG [H]

Identifier: 93004952

GI number: 93004952

Start: 144389

End: 145252

Strand: Direct

Name: mazG [H]

Synonym: Pcryo_0121

Alternate gene names: 93004952

Gene position: 144389-145252 (Clockwise)

Preceding gene: 93004950

Following gene: 93004953

Centisome position: 4.72

GC content: 45.49

Gene sequence:

>864_bases
ATGAATAGCTTAGAAAATAAAATAACAGCGCCTACACTGGTACAAGGAACGCCAGAAGCAAGTGGCGAATTAGCGGATTT
GTTGTCCTTAATGGCGCGGTTGCGTAGCGATTGTCCATGGGATAAAAAGCAAAGCAATCATAGCTTAATTCCGTATGCGA
TTGAAGAAGCCTATGAGCTCGGCGAAGCGGTACAAAGTAATGATGATGAAGACATCAAAGGCGAGCTTGGCGATGTGCTG
CTGCAAGTGGTTTTTCATTGTCAGATGTACGCTGAACAAGAGCGCTTTGATATGATTGATGTGATTACCACTTTACAAGA
AAAGCTGATTCGCCGGCATCCGCATGTGTTTGAAGCAGAAACGCTTAAAGATGAAGCCGCGGTAAAAGAACGCTGGGATG
AGATTAAAGTAGAAGAGCAACAAGCCCGCGCCGTACGTGGCAAACCACAGCGTCGTTTGGACAATACCAAAGCAGGTAGT
GCGCTGATGCAGGCGCAGGATGTGCAAAAACAGGCGTCAAAATTGGGTTTTGATTGGGAAGGCGTCAGTGGCGCATTTGA
TAAACTCGATGAAGAAATTGCCGAGCTAAAGACCGAGATTTTAGATAAGCCCCAAGTTGAAATCAAAACCAATATCAGAG
AGATTGAAAAGGAGCTTGGCGATTGCATGTTTGCGCTTGTCAATGTGGCGCGCAAATTAGACCTTGATGCGGAAACAGCA
ACGCTGACCTGTGTACATAAATTTAAATCACGTTTTGGCTATATTGAAGACCAGTTAGCTGCCGCAGGCAAGCGTTTAGA
AGACAGCGATATCAATGAGATGGATGCGTTATGGGAAGCGGCGAAACAACATGAACGCGACTAA

Upstream 100 bases:

>100_bases
TTATAAGCTAATCAGTGTTCAATGCTACACTAACGACAAAATCGTTATAAACTTAATAGGGTTCTATTGAATAACCAATA
AAAATATAAGAGTGTTTTTT

Downstream 100 bases:

>100_bases
TAAAAGCTTGCTTATTAAATGGTTAGCCATCAAATGGTCGTTTATTAAATGGCAGCATGCTAATTTGTTTAAAAATGTAG
CCATTTTTTTAGTAGGCATG

Product: nucleoside triphosphate pyrophosphohydrolase

Products: NA

Alternate protein names: NTP-PPase [H]

Number of amino acids: Translated: 287; Mature: 287

Protein sequence:

>287_residues
MNSLENKITAPTLVQGTPEASGELADLLSLMARLRSDCPWDKKQSNHSLIPYAIEEAYELGEAVQSNDDEDIKGELGDVL
LQVVFHCQMYAEQERFDMIDVITTLQEKLIRRHPHVFEAETLKDEAAVKERWDEIKVEEQQARAVRGKPQRRLDNTKAGS
ALMQAQDVQKQASKLGFDWEGVSGAFDKLDEEIAELKTEILDKPQVEIKTNIREIEKELGDCMFALVNVARKLDLDAETA
TLTCVHKFKSRFGYIEDQLAAAGKRLEDSDINEMDALWEAAKQHERD

Sequences:

>Translated_287_residues
MNSLENKITAPTLVQGTPEASGELADLLSLMARLRSDCPWDKKQSNHSLIPYAIEEAYELGEAVQSNDDEDIKGELGDVL
LQVVFHCQMYAEQERFDMIDVITTLQEKLIRRHPHVFEAETLKDEAAVKERWDEIKVEEQQARAVRGKPQRRLDNTKAGS
ALMQAQDVQKQASKLGFDWEGVSGAFDKLDEEIAELKTEILDKPQVEIKTNIREIEKELGDCMFALVNVARKLDLDAETA
TLTCVHKFKSRFGYIEDQLAAAGKRLEDSDINEMDALWEAAKQHERD
>Mature_287_residues
MNSLENKITAPTLVQGTPEASGELADLLSLMARLRSDCPWDKKQSNHSLIPYAIEEAYELGEAVQSNDDEDIKGELGDVL
LQVVFHCQMYAEQERFDMIDVITTLQEKLIRRHPHVFEAETLKDEAAVKERWDEIKVEEQQARAVRGKPQRRLDNTKAGS
ALMQAQDVQKQASKLGFDWEGVSGAFDKLDEEIAELKTEILDKPQVEIKTNIREIEKELGDCMFALVNVARKLDLDAETA
TLTCVHKFKSRFGYIEDQLAAAGKRLEDSDINEMDALWEAAKQHERD

Specific function: Involved in the regulation of bacterial cell survival under conditions of nutritional stress. Regulates the MazEF toxin- antitoxin (TA) module that mediates programmed cell death (PCD). This is achieved by lowering the cellular concentration of (p)ppGpp p

COG id: COG1694

COG function: function code R; Predicted pyrophosphatase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nucleoside triphosphate pyrophosphohydrolase family [H]

Homologues:

Organism=Escherichia coli, GI1789144, Length=268, Percent_Identity=38.8059701492537, Blast_Score=187, Evalue=7e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004518
- InterPro:   IPR011551 [H]

Pfam domain/function: PF03819 MazG [H]

EC number: =3.6.1.8 [H]

Molecular weight: Translated: 32574; Mature: 32574

Theoretical pI: Translated: 4.45; Mature: 4.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSLENKITAPTLVQGTPEASGELADLLSLMARLRSDCPWDKKQSNHSLIPYAIEEAYEL
CCCHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHH
GEAVQSNDDEDIKGELGDVLLQVVFHCQMYAEQERFDMIDVITTLQEKLIRRHPHVFEAE
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
TLKDEAAVKERWDEIKVEEQQARAVRGKPQRRLDNTKAGSALMQAQDVQKQASKLGFDWE
HHHHHHHHHHHHHHHCHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCC
GVSGAFDKLDEEIAELKTEILDKPQVEIKTNIREIEKELGDCMFALVNVARKLDLDAETA
CCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
TLTCVHKFKSRFGYIEDQLAAAGKRLEDSDINEMDALWEAAKQHERD
HHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNSLENKITAPTLVQGTPEASGELADLLSLMARLRSDCPWDKKQSNHSLIPYAIEEAYEL
CCCHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHH
GEAVQSNDDEDIKGELGDVLLQVVFHCQMYAEQERFDMIDVITTLQEKLIRRHPHVFEAE
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
TLKDEAAVKERWDEIKVEEQQARAVRGKPQRRLDNTKAGSALMQAQDVQKQASKLGFDWE
HHHHHHHHHHHHHHHCHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCC
GVSGAFDKLDEEIAELKTEILDKPQVEIKTNIREIEKELGDCMFALVNVARKLDLDAETA
CCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
TLTCVHKFKSRFGYIEDQLAAAGKRLEDSDINEMDALWEAAKQHERD
HHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]