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The map label for this gene is lpdG [H]

Identifier: 93004944

GI number: 93004944

Start: 133596

End: 135047

Strand: Direct

Name: lpdG [H]

Synonym: Pcryo_0113

Alternate gene names: 93004944

Gene position: 133596-135047 (Clockwise)

Preceding gene: 93004943

Following gene: 93004945

Centisome position: 4.37

GC content: 45.73

Gene sequence:

>1452_bases
ATGAAAGACAGTTACGATTTAGTCGTCATCGGCGGCGGACCTGGTGGTTATGAAGCAGCAATCCGTGCAGGACAGCTTGG
CATGAGCGTCGCTTGTATCGAAAAACGCGTTTATAAAGGCGAGCCTGCACTTGGCGGTACTTGCTTAAACGTCGGTTGTA
TCCCATCAAAAGCATTGCTTGATAGCTCACATCGCTATGAAGCGACTAAGCACGACCTTAATGAGCATGGTATTACTACT
GGTGATGTTGCTATCGATATCGAGCAAATGATTGCTCGTAAAGAAGGCATCGTTAAGCAGTTGACTGGCGGTGTTGCTGC
GTTATTGAAAGGTAATGGAGTTGACTGGCTACAAGGCTGGGGCACATTAGAAGATGGTAAAGGTACTGATAAAAAAGTTA
AATTTACCGCTCTTGCTGATGAAGCAGAAACGACTATCACTGCTAAAAACGTGATTCTTGCAGCTGGTTCAGTGCCGATT
GATATTCCAGTTGCCAGAACTGACGGCGATCGTATCGTTGATTCTACTGGCGCGCTTGACTTTACTGCTGTACCTAAGCG
TTTAGGCGTAATCGGTGCCGGTGTTATCGGTCTTGAGCTTGGTTCAGTATGGCGTCGTCTAGGTGCAGAAGTTGTCGTAT
ATGAAGCACTACCTAGCTTCTTGGCCGCTGCTGACAAAGACATCGCTAAAGAAGCGGGCAAAATGCTGAAAAAGCAAGGT
CTTGATATTCGTGTCGATACCAAAGTGACCAATGCTGAAGTGCAAGGCGATCAAGTCATCGTTACTAGTGAAACCAAAGG
CGAATCTAGTGAAGAGAGCTTTGATAAGCTGATCGTTTGTGTCGGTCGCCGTGCTTATTCTGAAAAATTACTTGGCGAAG
ACAGTGGTATTCAATTGACCGAACGCGGTTTGATTGATGTCAATGATCAGTGCAAAACCAATCTTGATGGCGTTTATGCT
ATCGGTGATTTGGTACGCGGTCCTATGCTTGCGCATAAAGCCATGGAAGAAGGCATGATGGCTGTTGAGCGTATTCATGG
CGAAAAAGCTCAAGTTAACTACGACACTATTATCAACGTCATCTATACGCATCCAGAAATCGCATGGGTTGGTTTGACAG
AGCAAGAAGCAGAAGTCGCAGGTTATGAAGTCAAAACAGGTTCATTTAACCTAGCTGCAAACGGTCGCGCTCTTGCTCAA
AGTGAAGCTCAAGGTTCTATCAAAGTCGTTGCTGATGCCAAAACAGACCGTTTATTAGGCATGCATGCGATTTCTGCTGG
TGCAGGCGATATCGTCCATCAAGGTATGATTGCGATGGAATTTGTATCAAGCATCGAAGATTTGCAGTTGATGACGTTTG
CGCATCCAACGATCTCTGAAGCCGTTCATGAAGCAGCATTGTCTGCGGATGGTCGTGCCATTCACGCGATTCAGCGTAAA
AAACGTAAATAA

Upstream 100 bases:

>100_bases
CGTATTGGTTATCGAATGGATAAGTCGATGGTTTACTAACGAGATAAAATTGGTTTATATGATACCTAACATTAAACTAA
AATTAAAATAGGAACGTATT

Downstream 100 bases:

>100_bases
TTTTAACGGCTAAAAATATTTGTATAAAGATATTTTAAGTGTTTTAAATGGTTATTAGATGATGGCATGACCATCGCATT
GTTCGAATAGCTATTTTGAA

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: E3 component of 2-oxoglutarate dehydrogenase complex; Glycine oxidation system L-factor; LPD-GLC [H]

Number of amino acids: Translated: 483; Mature: 483

Protein sequence:

>483_residues
MKDSYDLVVIGGGPGGYEAAIRAGQLGMSVACIEKRVYKGEPALGGTCLNVGCIPSKALLDSSHRYEATKHDLNEHGITT
GDVAIDIEQMIARKEGIVKQLTGGVAALLKGNGVDWLQGWGTLEDGKGTDKKVKFTALADEAETTITAKNVILAAGSVPI
DIPVARTDGDRIVDSTGALDFTAVPKRLGVIGAGVIGLELGSVWRRLGAEVVVYEALPSFLAAADKDIAKEAGKMLKKQG
LDIRVDTKVTNAEVQGDQVIVTSETKGESSEESFDKLIVCVGRRAYSEKLLGEDSGIQLTERGLIDVNDQCKTNLDGVYA
IGDLVRGPMLAHKAMEEGMMAVERIHGEKAQVNYDTIINVIYTHPEIAWVGLTEQEAEVAGYEVKTGSFNLAANGRALAQ
SEAQGSIKVVADAKTDRLLGMHAISAGAGDIVHQGMIAMEFVSSIEDLQLMTFAHPTISEAVHEAALSADGRAIHAIQRK
KRK

Sequences:

>Translated_483_residues
MKDSYDLVVIGGGPGGYEAAIRAGQLGMSVACIEKRVYKGEPALGGTCLNVGCIPSKALLDSSHRYEATKHDLNEHGITT
GDVAIDIEQMIARKEGIVKQLTGGVAALLKGNGVDWLQGWGTLEDGKGTDKKVKFTALADEAETTITAKNVILAAGSVPI
DIPVARTDGDRIVDSTGALDFTAVPKRLGVIGAGVIGLELGSVWRRLGAEVVVYEALPSFLAAADKDIAKEAGKMLKKQG
LDIRVDTKVTNAEVQGDQVIVTSETKGESSEESFDKLIVCVGRRAYSEKLLGEDSGIQLTERGLIDVNDQCKTNLDGVYA
IGDLVRGPMLAHKAMEEGMMAVERIHGEKAQVNYDTIINVIYTHPEIAWVGLTEQEAEVAGYEVKTGSFNLAANGRALAQ
SEAQGSIKVVADAKTDRLLGMHAISAGAGDIVHQGMIAMEFVSSIEDLQLMTFAHPTISEAVHEAALSADGRAIHAIQRK
KRK
>Mature_483_residues
MKDSYDLVVIGGGPGGYEAAIRAGQLGMSVACIEKRVYKGEPALGGTCLNVGCIPSKALLDSSHRYEATKHDLNEHGITT
GDVAIDIEQMIARKEGIVKQLTGGVAALLKGNGVDWLQGWGTLEDGKGTDKKVKFTALADEAETTITAKNVILAAGSVPI
DIPVARTDGDRIVDSTGALDFTAVPKRLGVIGAGVIGLELGSVWRRLGAEVVVYEALPSFLAAADKDIAKEAGKMLKKQG
LDIRVDTKVTNAEVQGDQVIVTSETKGESSEESFDKLIVCVGRRAYSEKLLGEDSGIQLTERGLIDVNDQCKTNLDGVYA
IGDLVRGPMLAHKAMEEGMMAVERIHGEKAQVNYDTIINVIYTHPEIAWVGLTEQEAEVAGYEVKTGSFNLAANGRALAQ
SEAQGSIKVVADAKTDRLLGMHAISAGAGDIVHQGMIAMEFVSSIEDLQLMTFAHPTISEAVHEAALSADGRAIHAIQRK
KRK

Specific function: Also acts in the glycine cleavage system [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=476, Percent_Identity=43.6974789915966, Blast_Score=377, Evalue=1e-104,
Organism=Homo sapiens, GI50301238, Length=470, Percent_Identity=25.531914893617, Blast_Score=136, Evalue=6e-32,
Organism=Homo sapiens, GI33519430, Length=435, Percent_Identity=24.5977011494253, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI33519428, Length=435, Percent_Identity=24.5977011494253, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI33519426, Length=435, Percent_Identity=24.5977011494253, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI148277065, Length=435, Percent_Identity=24.5977011494253, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI148277071, Length=435, Percent_Identity=24.5977011494253, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI291045266, Length=439, Percent_Identity=23.9179954441913, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI291045268, Length=430, Percent_Identity=23.953488372093, Blast_Score=93, Evalue=4e-19,
Organism=Homo sapiens, GI22035672, Length=496, Percent_Identity=25.6048387096774, Blast_Score=89, Evalue=1e-17,
Organism=Escherichia coli, GI1786307, Length=478, Percent_Identity=38.7029288702929, Blast_Score=312, Evalue=3e-86,
Organism=Escherichia coli, GI87081717, Length=466, Percent_Identity=26.3948497854077, Blast_Score=158, Evalue=6e-40,
Organism=Escherichia coli, GI87082354, Length=476, Percent_Identity=29.4117647058824, Blast_Score=158, Evalue=9e-40,
Organism=Escherichia coli, GI1789915, Length=445, Percent_Identity=27.1910112359551, Blast_Score=154, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI32565766, Length=476, Percent_Identity=43.6974789915966, Blast_Score=375, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI17557007, Length=479, Percent_Identity=26.0960334029228, Blast_Score=125, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI71983429, Length=451, Percent_Identity=26.8292682926829, Blast_Score=119, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI71983419, Length=451, Percent_Identity=26.8292682926829, Blast_Score=118, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI71982272, Length=493, Percent_Identity=24.7464503042596, Blast_Score=100, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6321091, Length=487, Percent_Identity=43.1211498973306, Blast_Score=371, Evalue=1e-103,
Organism=Saccharomyces cerevisiae, GI6325240, Length=477, Percent_Identity=28.7211740041929, Blast_Score=192, Evalue=1e-49,
Organism=Saccharomyces cerevisiae, GI6325166, Length=479, Percent_Identity=26.9311064718163, Blast_Score=136, Evalue=7e-33,
Organism=Drosophila melanogaster, GI21358499, Length=468, Percent_Identity=45.9401709401709, Blast_Score=392, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24640549, Length=482, Percent_Identity=27.5933609958506, Blast_Score=140, Evalue=1e-33,
Organism=Drosophila melanogaster, GI24640553, Length=482, Percent_Identity=27.5933609958506, Blast_Score=140, Evalue=1e-33,
Organism=Drosophila melanogaster, GI24640551, Length=482, Percent_Identity=27.5933609958506, Blast_Score=140, Evalue=2e-33,
Organism=Drosophila melanogaster, GI17737741, Length=475, Percent_Identity=26.1052631578947, Blast_Score=122, Evalue=7e-28,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 51301; Mature: 51301

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDSYDLVVIGGGPGGYEAAIRAGQLGMSVACIEKRVYKGEPALGGTCLNVGCIPSKALL
CCCCEEEEEEECCCCCHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHH
DSSHRYEATKHDLNEHGITTGDVAIDIEQMIARKEGIVKQLTGGVAALLKGNGVDWLQGW
CCCCCCCHHHHCHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHCCCEEEHCCCCCHHHHCC
GTLEDGKGTDKKVKFTALADEAETTITAKNVILAAGSVPIDIPVARTDGDRIVDSTGALD
CCCCCCCCCCCEEEEEEECCCCCCEEEECEEEEEECCCCEEEEEEECCCCEEECCCCCEE
FTAVPKRLGVIGAGVIGLELGSVWRRLGAEVVVYEALPSFLAAADKDIAKEAGKMLKKQG
EEHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHCC
LDIRVDTKVTNAEVQGDQVIVTSETKGESSEESFDKLIVCVGRRAYSEKLLGEDSGIQLT
CCEEEECEEECCEECCCEEEEEECCCCCCCHHHHHHHHHHHCCHHHHHHHCCCCCCCEEE
ERGLIDVNDQCKTNLDGVYAIGDLVRGPMLAHKAMEEGMMAVERIHGEKAQVNYDTIINV
CCCCEECCCHHCCCCCCEEEHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCEECHHHEEEE
IYTHPEIAWVGLTEQEAEVAGYEVKTGSFNLAANGRALAQSEAQGSIKVVADAKTDRLLG
EEECCCEEEEECCCCCHHHCCEEEECCCEEEEECCCHHHHCCCCCCEEEEEECCCCCCHH
MHAISAGAGDIVHQGMIAMEFVSSIEDLQLMTFAHPTISEAVHEAALSADGRAIHAIQRK
HHHHCCCCCHHHHHHHHHHHHHHHHHHHEEEEECCCHHHHHHHHHHHCCCCCHHHHHHHH
KRK
HCC
>Mature Secondary Structure
MKDSYDLVVIGGGPGGYEAAIRAGQLGMSVACIEKRVYKGEPALGGTCLNVGCIPSKALL
CCCCEEEEEEECCCCCHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHH
DSSHRYEATKHDLNEHGITTGDVAIDIEQMIARKEGIVKQLTGGVAALLKGNGVDWLQGW
CCCCCCCHHHHCHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHCCCEEEHCCCCCHHHHCC
GTLEDGKGTDKKVKFTALADEAETTITAKNVILAAGSVPIDIPVARTDGDRIVDSTGALD
CCCCCCCCCCCEEEEEEECCCCCCEEEECEEEEEECCCCEEEEEEECCCCEEECCCCCEE
FTAVPKRLGVIGAGVIGLELGSVWRRLGAEVVVYEALPSFLAAADKDIAKEAGKMLKKQG
EEHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHCC
LDIRVDTKVTNAEVQGDQVIVTSETKGESSEESFDKLIVCVGRRAYSEKLLGEDSGIQLT
CCEEEECEEECCEECCCEEEEEECCCCCCCHHHHHHHHHHHCCHHHHHHHCCCCCCCEEE
ERGLIDVNDQCKTNLDGVYAIGDLVRGPMLAHKAMEEGMMAVERIHGEKAQVNYDTIINV
CCCCEECCCHHCCCCCCEEEHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCEECHHHEEEE
IYTHPEIAWVGLTEQEAEVAGYEVKTGSFNLAANGRALAQSEAQGSIKVVADAKTDRLLG
EEECCCEEEEECCCCCHHHCCEEEECCCEEEEECCCHHHHCCCCCCEEEEEECCCCCCHH
MHAISAGAGDIVHQGMIAMEFVSSIEDLQLMTFAHPTISEAVHEAALSADGRAIHAIQRK
HHHHCCCCCHHHHHHHHHHHHHHHHHHHEEEEECCCHHHHHHHHHHHCCCCCHHHHHHHH
KRK
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1902462; 2914869 [H]