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The map label for this gene is sucB [H]

Identifier: 93004943

GI number: 93004943

Start: 132092

End: 133324

Strand: Direct

Name: sucB [H]

Synonym: Pcryo_0112

Alternate gene names: 93004943

Gene position: 132092-133324 (Clockwise)

Preceding gene: 93004942

Following gene: 93004944

Centisome position: 4.32

GC content: 44.93

Gene sequence:

>1233_bases
ATGGCTGAAATTAAAGCCCCCGTTTTTCCAGAATCGGTTGCCGATGGTACAATCGTTGAATGGCATGTCACTGAAGGTCA
GCAAGTAAACCGTGATGACCTATTAGCTGAAATCGAAACTGATAAAGTTGTATTAGAAGTGGTCGCGCCTGATAACGGTG
TTGTAACCCGAATCGTTAAGCAAGTAGATGATACCGTATTGTCTGATGAGCTGATTGCCGAATTTGAAGCTGGTGCTAGC
GCCAGTGCTGAAGCTGCTCCTGCCGTTGATCCAGATCAACCAGCTGCGCCAGTACAACCTAAGCAAGCAACAGATGGCGG
CGAGCCAGTACAAGCGTCTGCTGAATCTGATCATAAAGATCAAAGCCCTGCGGTTCGTAAAGCAGCCAAAGAGTCTGGCG
TAGATCCTAAAAACGTCGAAGGTAGTGGTCGCGGCGGTCGTGTGACCAAAACTGACATGTCAAACCCAACGTTAAAAGCG
GATAGCTCAATCACTTCTGATAGTGGTCGTCCTGTTGCTGAAGCAGTTGGCGAGCGTACTGAAAAACGCGTACCAATGAC
ACGTCTACGTAAAACAATCGCGAATCGTCTACTAGCTGCTTCTCAAGAAACGGCAATGTTAACGACGTTTAACGAAGTCA
ACATGAAACCGTTGATGGACATGCGTACTAAGTATAAAGATCAGTTCGAAAAGCGTCATGGTACACGTTTAGGCTTTATG
TCGTTATTCGTAAAAGCGGCGACTGAAGCCCTAAAACGTTATCCAGCTGTGAACGCGTCATTAGACGGTGATGATATCGT
TTATCATGGTTACTATGACATCGGTGTTGCCGTATCTTCTAACCGTGGTCTAGTCGTACCAGTACTGCGTGATACTGATC
GCATGAGCATGGCAGATGTTGAAGCCAAGATTCGTGAGTTTGGTGGTAAAGCACAAGAAGGTAAACTTGGTCTAGAAGAC
ATGGTTGGCGGTACATTTACCATCTCAAACGGTGGTGTATTTGGTTCACTGATGTCAACACCTATCTTGAACCCACCACA
AACTGCTATCTTGGGTATGCATGCTATCAATGACCGTCCTATGGCAGTTAATGGTGAAGTTAAAATCTTGCCAATGATGT
ATCTTGCACTGTCTTATGACCATCGTATGATTGATGGTAAAGAAGCGGTACAATTCTTAGTAACACTGAAAGAATTGGTT
GAAGACCCTACTATGCTACTTTTAGATCTTTAA

Upstream 100 bases:

>100_bases
GTACCCAGTGAGACAATTTAAATATCACATTTTAAATGGCACTGACTGCCTATCACGACTTAATTATAAATAGTAATATC
CAAATCTAAGGAGTATATCG

Downstream 100 bases:

>100_bases
GTTTTTAGGCTTGTATCAGTAATGACTGATACAAGCTTTTTAGCTTTGTATAGATTATCTTTTAATGCCTTTTAGCTTTT
TCAGTTCTATCTTATGAGCA

Product: 2-oxoglutarate dehydrogenase, E2 component, dihydrolipoamide succinyltransferase

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 410; Mature: 409

Protein sequence:

>410_residues
MAEIKAPVFPESVADGTIVEWHVTEGQQVNRDDLLAEIETDKVVLEVVAPDNGVVTRIVKQVDDTVLSDELIAEFEAGAS
ASAEAAPAVDPDQPAAPVQPKQATDGGEPVQASAESDHKDQSPAVRKAAKESGVDPKNVEGSGRGGRVTKTDMSNPTLKA
DSSITSDSGRPVAEAVGERTEKRVPMTRLRKTIANRLLAASQETAMLTTFNEVNMKPLMDMRTKYKDQFEKRHGTRLGFM
SLFVKAATEALKRYPAVNASLDGDDIVYHGYYDIGVAVSSNRGLVVPVLRDTDRMSMADVEAKIREFGGKAQEGKLGLED
MVGGTFTISNGGVFGSLMSTPILNPPQTAILGMHAINDRPMAVNGEVKILPMMYLALSYDHRMIDGKEAVQFLVTLKELV
EDPTMLLLDL

Sequences:

>Translated_410_residues
MAEIKAPVFPESVADGTIVEWHVTEGQQVNRDDLLAEIETDKVVLEVVAPDNGVVTRIVKQVDDTVLSDELIAEFEAGAS
ASAEAAPAVDPDQPAAPVQPKQATDGGEPVQASAESDHKDQSPAVRKAAKESGVDPKNVEGSGRGGRVTKTDMSNPTLKA
DSSITSDSGRPVAEAVGERTEKRVPMTRLRKTIANRLLAASQETAMLTTFNEVNMKPLMDMRTKYKDQFEKRHGTRLGFM
SLFVKAATEALKRYPAVNASLDGDDIVYHGYYDIGVAVSSNRGLVVPVLRDTDRMSMADVEAKIREFGGKAQEGKLGLED
MVGGTFTISNGGVFGSLMSTPILNPPQTAILGMHAINDRPMAVNGEVKILPMMYLALSYDHRMIDGKEAVQFLVTLKELV
EDPTMLLLDL
>Mature_409_residues
AEIKAPVFPESVADGTIVEWHVTEGQQVNRDDLLAEIETDKVVLEVVAPDNGVVTRIVKQVDDTVLSDELIAEFEAGASA
SAEAAPAVDPDQPAAPVQPKQATDGGEPVQASAESDHKDQSPAVRKAAKESGVDPKNVEGSGRGGRVTKTDMSNPTLKAD
SSITSDSGRPVAEAVGERTEKRVPMTRLRKTIANRLLAASQETAMLTTFNEVNMKPLMDMRTKYKDQFEKRHGTRLGFMS
LFVKAATEALKRYPAVNASLDGDDIVYHGYYDIGVAVSSNRGLVVPVLRDTDRMSMADVEAKIREFGGKAQEGKLGLEDM
VGGTFTISNGGVFGSLMSTPILNPPQTAILGMHAINDRPMAVNGEVKILPMMYLALSYDHRMIDGKEAVQFLVTLKELVE
DPTMLLLDL

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components:2- oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2)

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI19923748, Length=245, Percent_Identity=56.3265306122449, Blast_Score=283, Evalue=2e-76,
Organism=Homo sapiens, GI110671329, Length=425, Percent_Identity=27.2941176470588, Blast_Score=164, Evalue=2e-40,
Organism=Homo sapiens, GI31711992, Length=444, Percent_Identity=28.6036036036036, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI203098753, Length=455, Percent_Identity=25.9340659340659, Blast_Score=125, Evalue=8e-29,
Organism=Homo sapiens, GI203098816, Length=454, Percent_Identity=25.7709251101322, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI260898739, Length=161, Percent_Identity=31.6770186335404, Blast_Score=89, Evalue=5e-18,
Organism=Escherichia coli, GI1786946, Length=411, Percent_Identity=55.7177615571776, Blast_Score=450, Evalue=1e-128,
Organism=Escherichia coli, GI1786305, Length=412, Percent_Identity=28.3980582524272, Blast_Score=134, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI25146366, Length=410, Percent_Identity=42.9268292682927, Blast_Score=314, Evalue=4e-86,
Organism=Caenorhabditis elegans, GI17560088, Length=435, Percent_Identity=31.264367816092, Blast_Score=175, Evalue=4e-44,
Organism=Caenorhabditis elegans, GI17537937, Length=422, Percent_Identity=25.5924170616114, Blast_Score=149, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI17538894, Length=259, Percent_Identity=31.2741312741313, Blast_Score=133, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6320352, Length=410, Percent_Identity=45.1219512195122, Blast_Score=330, Evalue=2e-91,
Organism=Saccharomyces cerevisiae, GI6324258, Length=449, Percent_Identity=24.9443207126949, Blast_Score=129, Evalue=6e-31,
Organism=Drosophila melanogaster, GI24645909, Length=237, Percent_Identity=56.5400843881857, Blast_Score=278, Evalue=6e-75,
Organism=Drosophila melanogaster, GI18859875, Length=426, Percent_Identity=26.7605633802817, Blast_Score=153, Evalue=2e-37,
Organism=Drosophila melanogaster, GI24582497, Length=229, Percent_Identity=28.82096069869, Blast_Score=118, Evalue=7e-27,
Organism=Drosophila melanogaster, GI20129315, Length=229, Percent_Identity=28.82096069869, Blast_Score=117, Evalue=1e-26,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- InterPro:   IPR006255 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 44267; Mature: 44136

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEIKAPVFPESVADGTIVEWHVTEGQQVNRDDLLAEIETDKVVLEVVAPDNGVVTRIVK
CCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHEEECCCEEEEEEECCCCCHHHHHHH
QVDDTVLSDELIAEFEAGASASAEAAPAVDPDQPAAPVQPKQATDGGEPVQASAESDHKD
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
QSPAVRKAAKESGVDPKNVEGSGRGGRVTKTDMSNPTLKADSSITSDSGRPVAEAVGERT
CCHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCCCEECCCCCCCCCCCHHHHHHHHHH
EKRVPMTRLRKTIANRLLAASQETAMLTTFNEVNMKPLMDMRTKYKDQFEKRHGTRLGFM
HHCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCHHHH
SLFVKAATEALKRYPAVNASLDGDDIVYHGYYDIGVAVSSNRGLVVPVLRDTDRMSMADV
HHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEEEEEECCCCEEEEEECCCCCCHHHHH
EAKIREFGGKAQEGKLGLEDMVGGTFTISNGGVFGSLMSTPILNPPQTAILGMHAINDRP
HHHHHHHCCCCCCCCCCHHHHCCCEEEECCCCEEHHHHHCCCCCCCHHHEEEEEECCCCC
MAVNGEVKILPMMYLALSYDHRMIDGKEAVQFLVTLKELVEDPTMLLLDL
EEECCCEEEHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEC
>Mature Secondary Structure 
AEIKAPVFPESVADGTIVEWHVTEGQQVNRDDLLAEIETDKVVLEVVAPDNGVVTRIVK
CCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHEEECCCEEEEEEECCCCCHHHHHHH
QVDDTVLSDELIAEFEAGASASAEAAPAVDPDQPAAPVQPKQATDGGEPVQASAESDHKD
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
QSPAVRKAAKESGVDPKNVEGSGRGGRVTKTDMSNPTLKADSSITSDSGRPVAEAVGERT
CCHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCCCEECCCCCCCCCCCHHHHHHHHHH
EKRVPMTRLRKTIANRLLAASQETAMLTTFNEVNMKPLMDMRTKYKDQFEKRHGTRLGFM
HHCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCHHHH
SLFVKAATEALKRYPAVNASLDGDDIVYHGYYDIGVAVSSNRGLVVPVLRDTDRMSMADV
HHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEEEEEECCCCEEEEEECCCCCCHHHHH
EAKIREFGGKAQEGKLGLEDMVGGTFTISNGGVFGSLMSTPILNPPQTAILGMHAINDRP
HHHHHHHCCCCCCCCCCHHHHCCCEEEECCCCEEHHHHHCCCCCCCHHHEEEEEECCCCC
MAVNGEVKILPMMYLALSYDHRMIDGKEAVQFLVTLKELVEDPTMLLLDL
EEECCCEEEHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]