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The map label for this gene is recF [H]

Identifier: 93004836

GI number: 93004836

Start: 4313

End: 5521

Strand: Direct

Name: recF [H]

Synonym: Pcryo_0005

Alternate gene names: 93004836

Gene position: 4313-5521 (Clockwise)

Preceding gene: 93004835

Following gene: 93004837

Centisome position: 0.14

GC content: 40.86

Gene sequence:

>1209_bases
ATGATTGAACGTCTACAAATCTCTCACTTGCGCAATTTAACGCACATCAATTTATCGCCTGCAGCTTGTAATGTTATTAT
CGGCGCTAATGGCAGTGGTAAAACCTCGTTACTTGAAGCTATATTCTTGTTATCAAGAGGCAAAAGTTTCCGTCATCATC
AGCCTAAACGCTATATCCAACATTATCAAGAGTCTGCCACTATACATGCTAATCTCAATGACAGCCGTACCTTAGCTATC
CAAAAGAAAGCAGATGCAACGACTATTCTGCGTTTAAATCAAACCACCGTTTACAACCAAAGCATCTTGACCGAGCAGCT
ACCAACATTGCTTATAGATCCATCAACGATGGACATGCTAGAGCAGGGTAGTGCCAGTCGTCGCCAGTTATTGGATTGGT
TAGTGTTTCACATGAAACAAGGCTTCCATTCCCAGTGGGTTGCTTATCAACGTCTTCTAAAACAGCGCAATAGTTTGTTA
AAGCAACGGCGTCATTTGACTCAGGTTCAGCTTGCTGAGCTTAAGGCTTGGGATAAAGGGCTTTCCAGTCATGCAGCGCT
GATTCATCACTACCGTCAAGCTATATTTGAAGCTTGGCAGCCTTATTTTTCTAAGAGCATTGCACAGTTATTGCCTGCTT
ATGCTGAGCAGTTAAGCTTAAGTTACAATGCGGGCTACGATACTGGTATAGCGCTCGACATACAGCTTAATGAACGCCTA
GAGCAGGATTTGCAGTTGGGTTATACCCGTATTGGTAACCATCGTGCTGATATTCACGTGCATTGGCGCTCGATAGGCTC
AAGACAGATAGCTGATGAGAATCTAAACTCACCTTTATCAGCGGATTCGACTGTTAAACTGCCTATTTTAAAAGAGCAAG
CAGCTAATATTTTATCTCGTGGAGAAAAAAAACTATTGATTACGGCATTGCGTTTATCACAATTACCATTGTTGCTTAAT
GCTAAAACTAATAGCGAGCTATATAACAGTGACGCTAAATTAAGTGCTACACCAGTGGTATTACTAGATGATATTACCGC
AGAGCTAGATAATAAGGCGATAGAGATTTTATTATCAACATTAGCACAGCTACCATGTCAGGTATTTGTGACCAGTTTGA
CAGACAGTATTTTACCTTTAGTACATGAATATTGGGAAAAATCTCAAGTGTTTCACATGAAACAAGGAGGTATTTTACCA
GCTCAGTAG

Upstream 100 bases:

>100_bases
ATATCGATAATTTTAAGCTTCCAAAGCTTAAAATCTTATATTTTAATTAAACTTTTGCCTGCTAAAATACAAGCAGGATT
TATAGGCGGCTGTCGGTATT

Downstream 100 bases:

>100_bases
CTTTTACTAGGGTATTGATATCTGCGTTATTAAGCCTTATTTTATGTCCTTAAGCGCCCGTCCTCTTGCATATTTCACCC
TCATCTTATACCGCATTCTG

Product: DNA replication and repair protein RecF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 402; Mature: 402

Protein sequence:

>402_residues
MIERLQISHLRNLTHINLSPAACNVIIGANGSGKTSLLEAIFLLSRGKSFRHHQPKRYIQHYQESATIHANLNDSRTLAI
QKKADATTILRLNQTTVYNQSILTEQLPTLLIDPSTMDMLEQGSASRRQLLDWLVFHMKQGFHSQWVAYQRLLKQRNSLL
KQRRHLTQVQLAELKAWDKGLSSHAALIHHYRQAIFEAWQPYFSKSIAQLLPAYAEQLSLSYNAGYDTGIALDIQLNERL
EQDLQLGYTRIGNHRADIHVHWRSIGSRQIADENLNSPLSADSTVKLPILKEQAANILSRGEKKLLITALRLSQLPLLLN
AKTNSELYNSDAKLSATPVVLLDDITAELDNKAIEILLSTLAQLPCQVFVTSLTDSILPLVHEYWEKSQVFHMKQGGILP
AQ

Sequences:

>Translated_402_residues
MIERLQISHLRNLTHINLSPAACNVIIGANGSGKTSLLEAIFLLSRGKSFRHHQPKRYIQHYQESATIHANLNDSRTLAI
QKKADATTILRLNQTTVYNQSILTEQLPTLLIDPSTMDMLEQGSASRRQLLDWLVFHMKQGFHSQWVAYQRLLKQRNSLL
KQRRHLTQVQLAELKAWDKGLSSHAALIHHYRQAIFEAWQPYFSKSIAQLLPAYAEQLSLSYNAGYDTGIALDIQLNERL
EQDLQLGYTRIGNHRADIHVHWRSIGSRQIADENLNSPLSADSTVKLPILKEQAANILSRGEKKLLITALRLSQLPLLLN
AKTNSELYNSDAKLSATPVVLLDDITAELDNKAIEILLSTLAQLPCQVFVTSLTDSILPLVHEYWEKSQVFHMKQGGILP
AQ
>Mature_402_residues
MIERLQISHLRNLTHINLSPAACNVIIGANGSGKTSLLEAIFLLSRGKSFRHHQPKRYIQHYQESATIHANLNDSRTLAI
QKKADATTILRLNQTTVYNQSILTEQLPTLLIDPSTMDMLEQGSASRRQLLDWLVFHMKQGFHSQWVAYQRLLKQRNSLL
KQRRHLTQVQLAELKAWDKGLSSHAALIHHYRQAIFEAWQPYFSKSIAQLLPAYAEQLSLSYNAGYDTGIALDIQLNERL
EQDLQLGYTRIGNHRADIHVHWRSIGSRQIADENLNSPLSADSTVKLPILKEQAANILSRGEKKLLITALRLSQLPLLLN
AKTNSELYNSDAKLSATPVVLLDDITAELDNKAIEILLSTLAQLPCQVFVTSLTDSILPLVHEYWEKSQVFHMKQGGILP
AQ

Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP [H]

COG id: COG1195

COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recF family [H]

Homologues:

Organism=Escherichia coli, GI1790135, Length=403, Percent_Identity=31.7617866004963, Blast_Score=169, Evalue=2e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001238
- InterPro:   IPR003395 [H]

Pfam domain/function: PF02463 SMC_N [H]

EC number: NA

Molecular weight: Translated: 45461; Mature: 45461

Theoretical pI: Translated: 9.49; Mature: 9.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIERLQISHLRNLTHINLSPAACNVIIGANGSGKTSLLEAIFLLSRGKSFRHHQPKRYIQ
CCCCHHHHHHCCCCEEECCHHHEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHH
HYQESATIHANLNDSRTLAIQKKADATTILRLNQTTVYNQSILTEQLPTLLIDPSTMDML
HHHHCCEEEECCCCCEEEEEEECCCCEEEEEECCCCHHHHHHHHHHCCCEEECCCHHHHH
EQGSASRRQLLDWLVFHMKQGFHSQWVAYQRLLKQRNSLLKQRRHLTQVQLAELKAWDKG
HCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
LSSHAALIHHYRQAIFEAWQPYFSKSIAQLLPAYAEQLSLSYNAGYDTGIALDIQLNERL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEHHHHH
EQDLQLGYTRIGNHRADIHVHWRSIGSRQIADENLNSPLSADSTVKLPILKEQAANILSR
HHHHHHHHHHHCCCEEEEEEEEECCCCCHHCCCCCCCCCCCCCEEECCCHHHHHHHHHHC
GEKKLLITALRLSQLPLLLNAKTNSELYNSDAKLSATPVVLLDDITAELDNKAIEILLST
CCHHHHHHHHHHHCCCEEEECCCCCHHHCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHH
LAQLPCQVFVTSLTDSILPLVHEYWEKSQVFHMKQGGILPAQ
HHHCCHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCCCCCC
>Mature Secondary Structure
MIERLQISHLRNLTHINLSPAACNVIIGANGSGKTSLLEAIFLLSRGKSFRHHQPKRYIQ
CCCCHHHHHHCCCCEEECCHHHEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHH
HYQESATIHANLNDSRTLAIQKKADATTILRLNQTTVYNQSILTEQLPTLLIDPSTMDML
HHHHCCEEEECCCCCEEEEEEECCCCEEEEEECCCCHHHHHHHHHHCCCEEECCCHHHHH
EQGSASRRQLLDWLVFHMKQGFHSQWVAYQRLLKQRNSLLKQRRHLTQVQLAELKAWDKG
HCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
LSSHAALIHHYRQAIFEAWQPYFSKSIAQLLPAYAEQLSLSYNAGYDTGIALDIQLNERL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEHHHHH
EQDLQLGYTRIGNHRADIHVHWRSIGSRQIADENLNSPLSADSTVKLPILKEQAANILSR
HHHHHHHHHHHCCCEEEEEEEEECCCCCHHCCCCCCCCCCCCCEEECCCHHHHHHHHHHC
GEKKLLITALRLSQLPLLLNAKTNSELYNSDAKLSATPVVLLDDITAELDNKAIEILLST
CCHHHHHHHHHHHCCCEEEECCCCCHHHCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHH
LAQLPCQVFVTSLTDSILPLVHEYWEKSQVFHMKQGGILPAQ
HHHCCHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA