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The map label for this gene is gyrB [H]

Identifier: 93004837

GI number: 93004837

Start: 6021

End: 8636

Strand: Direct

Name: gyrB [H]

Synonym: Pcryo_0006

Alternate gene names: 93004837

Gene position: 6021-8636 (Clockwise)

Preceding gene: 93004836

Following gene: 93004838

Centisome position: 0.2

GC content: 43.62

Gene sequence:

>2616_bases
ATGAGTGACTCATTACCTACCGACCACCAGCAATTAACGTCAGACAGCGCAGTTGAGACTATTGCCGCCGCTGTCGCTCC
TGAGGTATTACCCTCTGATTACAGCTCGAAAGACATCCGAGTACTGCGCGGACTAGAAGCGGTGCGCGTGCGTCCTGGTA
TGTATATTGGTGATACCGATGATGGTACTGGCTTGCATCATATGGTCTTTGAGGTGGTGGATAACTCTATCGATGAAGCG
CTTGCTGGTCATTGTGATCAAATTGATATCATTATCCACGAAGATGAATCTGTCAGCGTGATGGATAATGGTCGCGGTGT
CCCTGTTGACATTCATCCAGAAGAAGGTGTGTCTGCCGCACAGGTTATTATGACGGTACTGCATGCTGGTGGTAAGTTTG
ATGATAATAGCTATAAAGTCTCTGGCGGTCTACATGGCGTTGGCGTTTCAGTCGTTAATGCATTATCCAAAAAGCTTGAG
ATGAATATCTGGCGTGAAGGTTATCATTATCATCAGACTTATACTGATGGTGTGCCTGATGGCGATATTCAGCAAATGGA
AGCGACAGATCAAACTGGCACACAAATCCGTTTTTACCCTAGCAATGACGTATTTACCGGTACTATTTTTGAATATGATA
TTTTAGCCAAGCGTTTACGTGAGCTGTCATTTTTGAACTCAGGCGTACGTATTGTTTTGACTGATGAACGTATTGATAAG
CGTCATGAGTTTGAACACAAAGGTGGATTGTCAGAGTTTGTCAGCTATATTAATGCTGGTAAAGATGGCATCAATGATGT
GTTCCATTTTATCAGTAATCAAGATGATGGTATCAGTGTCGAAGTGGCGTTGCAGTGGACAGATACTTATAACGAAAAGG
TGTTTTGTTTTACCAATAACATTCCACAACGTGATGGCGGCACGCATTTATCTGGCTTCCGCTCAGCACTCACGCGTTGT
TTGAATACCTATATGGACCGTGAAAATCTTTTCAAAAAAGAGAAAGTGGCTGCGACTGGTGATGATGCCCGTGAAGGTCT
AACGGCTATCGTATCTGTTAAAGTTCCAGATCCTAAGTTTTCAAGTCAAACCAAAGATAAGTTGGTATCAAGTGAAGTAA
AATCTGCCGTCGAATCAGCAATGCACGATAAGTTTAATGATTATCTGCAAGAAAATCCAAGCGCTGGTAAATTAATTGCC
GGTAAAATCATTGATGCTGCTCGTGCTCGTGATGCCGCTCGTAAAGCGCGTGAGATGACTCGTCGTAAGACCACATTAGA
TATCGCAGGTCTGCCGGGTAAATTGGCAGATTGCCAAGAAAAAGATCCTGCATTATCAGAGCTATACATTGTAGAAGGGG
ACTCTGCTGGCGGTTCAGCCAAGCAAGGTCGTAGCCGTAAAACGCAAGCAATTTTGCCTTTAAAAGGTAAAATCCTAAAC
GTTGAGCGCGCGCGTTTTGACAAGATGTTATCGTCACAAGAAGTCGGTAATCTAATTACTGCGCTTGGTTGTGGTATTGG
TCCTGATGAATATAATCCTGATAAAGTTCGCTATCATAAAATCATCATCATGACCGATGCGGACGTTGATGGTTCACATA
TTCGTACCCTTTTATTAACCTTTTTCTTCCGTCAAACGCCTGAATTGATTGAACGTGGTTATATTTATATTGCTCAGCCA
CCGCTTTATAAAGTCAAAAAAGGTCGTCAAGAGCTGTATCTAAAAGATGATGAGGCACTTAAAGCGTATTTATTATCTTC
AACGATTGATAATACTAGGCTGCATATCAGTGCTGATGCTCCTGCGATTACTGGTCAAGCGTTAGAGACTTTATTAAACG
ATTATAATCAAACGCAGCTTATTAAAGCGCGTTTGCAGATTCGTTATCCAGCCGTTTTGTTGGATGCCCTAACGCATACA
CCAAAGCTTAGTACTGATATTACGTATGATAAAGATGCAATGCAAGCATGGAAAGACACGCTACAGACCCAGCTTGAGCA
CTTTGGTAGTGACTTAAATCCTGAGATTGAGCTGGTGAATATCCATGCACCACAGCCTAAAAATATGGATGCCGCTGCCG
CTGATTTACTGGGTATGAGACCTGTCGTTGCCGTTGAAGAAAGTGATAGCTCTGACACTGAAGCGGTAGTCGCAAAAGCA
CAATGGCTGCCACGTATTACGGTCTATGTGCATCAGTTGGCCCATCATTATTTGCTCGATGCCAGCTTTATCAATTCGGG
CGAGTATACTAAGCTGCTGCGCTTATCAGCTGAATGGAATACCTTGCTTGATGAAGATGCCTTTATCCGCCGTGACGCAA
CAGCGGGTACAACCAAAGACATCCCAATACGTGACTTCGATTATTTGTGGCAGCAAATGATGCTTGACGCGCGTCGTGGT
CTAGCGATCCAGCGCTATAAAGGATTGGGCGAGATGAATGCCGATCAGCTGTGGGATACTACCATGGATCCTGAAAACCG
CCGTATGCTGCGCGTCAATATTGAAGATGCTATCGCAGCGGATCATATGTTCACCTGCTTGATGGGTGATCATGTAGAGC
CGCGTCGTATCTTTATCGAAGAAAATGCGTTAACGGTTTCTAATTTAGATATCTAA

Upstream 100 bases:

>100_bases
AATATGACTACAAAACGATATTATAGATAGCATCATTAAGTATCACCACTGTGGTGAGCTGATGATAAACAGCTGATAAT
GGCTAGATCAAGGAATGCAC

Downstream 100 bases:

>100_bases
ATGGCATAATATTGTGAATCAATGAAAAACCACCGCTTTGGCAACCAATGCGGTGGTTTTTTAATATTAAGCTTGTTTCA
CGTGAAACTTAATTGTATAA

Product: DNA gyrase, B subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 871; Mature: 870

Protein sequence:

>871_residues
MSDSLPTDHQQLTSDSAVETIAAAVAPEVLPSDYSSKDIRVLRGLEAVRVRPGMYIGDTDDGTGLHHMVFEVVDNSIDEA
LAGHCDQIDIIIHEDESVSVMDNGRGVPVDIHPEEGVSAAQVIMTVLHAGGKFDDNSYKVSGGLHGVGVSVVNALSKKLE
MNIWREGYHYHQTYTDGVPDGDIQQMEATDQTGTQIRFYPSNDVFTGTIFEYDILAKRLRELSFLNSGVRIVLTDERIDK
RHEFEHKGGLSEFVSYINAGKDGINDVFHFISNQDDGISVEVALQWTDTYNEKVFCFTNNIPQRDGGTHLSGFRSALTRC
LNTYMDRENLFKKEKVAATGDDAREGLTAIVSVKVPDPKFSSQTKDKLVSSEVKSAVESAMHDKFNDYLQENPSAGKLIA
GKIIDAARARDAARKAREMTRRKTTLDIAGLPGKLADCQEKDPALSELYIVEGDSAGGSAKQGRSRKTQAILPLKGKILN
VERARFDKMLSSQEVGNLITALGCGIGPDEYNPDKVRYHKIIIMTDADVDGSHIRTLLLTFFFRQTPELIERGYIYIAQP
PLYKVKKGRQELYLKDDEALKAYLLSSTIDNTRLHISADAPAITGQALETLLNDYNQTQLIKARLQIRYPAVLLDALTHT
PKLSTDITYDKDAMQAWKDTLQTQLEHFGSDLNPEIELVNIHAPQPKNMDAAAADLLGMRPVVAVEESDSSDTEAVVAKA
QWLPRITVYVHQLAHHYLLDASFINSGEYTKLLRLSAEWNTLLDEDAFIRRDATAGTTKDIPIRDFDYLWQQMMLDARRG
LAIQRYKGLGEMNADQLWDTTMDPENRRMLRVNIEDAIAADHMFTCLMGDHVEPRRIFIEENALTVSNLDI

Sequences:

>Translated_871_residues
MSDSLPTDHQQLTSDSAVETIAAAVAPEVLPSDYSSKDIRVLRGLEAVRVRPGMYIGDTDDGTGLHHMVFEVVDNSIDEA
LAGHCDQIDIIIHEDESVSVMDNGRGVPVDIHPEEGVSAAQVIMTVLHAGGKFDDNSYKVSGGLHGVGVSVVNALSKKLE
MNIWREGYHYHQTYTDGVPDGDIQQMEATDQTGTQIRFYPSNDVFTGTIFEYDILAKRLRELSFLNSGVRIVLTDERIDK
RHEFEHKGGLSEFVSYINAGKDGINDVFHFISNQDDGISVEVALQWTDTYNEKVFCFTNNIPQRDGGTHLSGFRSALTRC
LNTYMDRENLFKKEKVAATGDDAREGLTAIVSVKVPDPKFSSQTKDKLVSSEVKSAVESAMHDKFNDYLQENPSAGKLIA
GKIIDAARARDAARKAREMTRRKTTLDIAGLPGKLADCQEKDPALSELYIVEGDSAGGSAKQGRSRKTQAILPLKGKILN
VERARFDKMLSSQEVGNLITALGCGIGPDEYNPDKVRYHKIIIMTDADVDGSHIRTLLLTFFFRQTPELIERGYIYIAQP
PLYKVKKGRQELYLKDDEALKAYLLSSTIDNTRLHISADAPAITGQALETLLNDYNQTQLIKARLQIRYPAVLLDALTHT
PKLSTDITYDKDAMQAWKDTLQTQLEHFGSDLNPEIELVNIHAPQPKNMDAAAADLLGMRPVVAVEESDSSDTEAVVAKA
QWLPRITVYVHQLAHHYLLDASFINSGEYTKLLRLSAEWNTLLDEDAFIRRDATAGTTKDIPIRDFDYLWQQMMLDARRG
LAIQRYKGLGEMNADQLWDTTMDPENRRMLRVNIEDAIAADHMFTCLMGDHVEPRRIFIEENALTVSNLDI
>Mature_870_residues
SDSLPTDHQQLTSDSAVETIAAAVAPEVLPSDYSSKDIRVLRGLEAVRVRPGMYIGDTDDGTGLHHMVFEVVDNSIDEAL
AGHCDQIDIIIHEDESVSVMDNGRGVPVDIHPEEGVSAAQVIMTVLHAGGKFDDNSYKVSGGLHGVGVSVVNALSKKLEM
NIWREGYHYHQTYTDGVPDGDIQQMEATDQTGTQIRFYPSNDVFTGTIFEYDILAKRLRELSFLNSGVRIVLTDERIDKR
HEFEHKGGLSEFVSYINAGKDGINDVFHFISNQDDGISVEVALQWTDTYNEKVFCFTNNIPQRDGGTHLSGFRSALTRCL
NTYMDRENLFKKEKVAATGDDAREGLTAIVSVKVPDPKFSSQTKDKLVSSEVKSAVESAMHDKFNDYLQENPSAGKLIAG
KIIDAARARDAARKAREMTRRKTTLDIAGLPGKLADCQEKDPALSELYIVEGDSAGGSAKQGRSRKTQAILPLKGKILNV
ERARFDKMLSSQEVGNLITALGCGIGPDEYNPDKVRYHKIIIMTDADVDGSHIRTLLLTFFFRQTPELIERGYIYIAQPP
LYKVKKGRQELYLKDDEALKAYLLSSTIDNTRLHISADAPAITGQALETLLNDYNQTQLIKARLQIRYPAVLLDALTHTP
KLSTDITYDKDAMQAWKDTLQTQLEHFGSDLNPEIELVNIHAPQPKNMDAAAADLLGMRPVVAVEESDSSDTEAVVAKAQ
WLPRITVYVHQLAHHYLLDASFINSGEYTKLLRLSAEWNTLLDEDAFIRRDATAGTTKDIPIRDFDYLWQQMMLDARRGL
AIQRYKGLGEMNADQLWDTTMDPENRRMLRVNIEDAIAADHMFTCLMGDHVEPRRIFIEENALTVSNLDI

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI19913406, Length=574, Percent_Identity=25.9581881533101, Blast_Score=142, Evalue=1e-33,
Organism=Homo sapiens, GI19913408, Length=595, Percent_Identity=25.546218487395, Blast_Score=142, Evalue=1e-33,
Organism=Escherichia coli, GI48994957, Length=846, Percent_Identity=61.1111111111111, Blast_Score=1016, Evalue=0.0,
Organism=Escherichia coli, GI1789408, Length=555, Percent_Identity=40.7207207207207, Blast_Score=400, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI17535065, Length=570, Percent_Identity=24.2105263157895, Blast_Score=138, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI212645845, Length=523, Percent_Identity=25.2390057361377, Blast_Score=128, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI212645657, Length=161, Percent_Identity=32.9192546583851, Blast_Score=73, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6324241, Length=574, Percent_Identity=24.0418118466899, Blast_Score=114, Evalue=6e-26,
Organism=Drosophila melanogaster, GI17136538, Length=578, Percent_Identity=25.9515570934256, Blast_Score=145, Evalue=1e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR006171 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; PF01751 Toprim [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 97186; Mature: 97055

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDSLPTDHQQLTSDSAVETIAAAVAPEVLPSDYSSKDIRVLRGLEAVRVRPGMYIGDTD
CCCCCCCCHHHHCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCHHEEECCCEEECCCC
DGTGLHHMVFEVVDNSIDEALAGHCDQIDIIIHEDESVSVMDNGRGVPVDIHPEEGVSAA
CCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEECCCCCEEEECCCCCCHHH
QVIMTVLHAGGKFDDNSYKVSGGLHGVGVSVVNALSKKLEMNIWREGYHYHQTYTDGVPD
HHHHHHHHCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCEECCEECCCCCC
GDIQQMEATDQTGTQIRFYPSNDVFTGTIFEYDILAKRLRELSFLNSGVRIVLTDERIDK
CCHHHHHCCCCCCCEEEEEECCCEEEEEHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHH
RHEFEHKGGLSEFVSYINAGKDGINDVFHFISNQDDGISVEVALQWTDTYNEKVFCFTNN
HHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHCCCCCCEEEEEEEEEECCCCCEEEEEECC
IPQRDGGTHLSGFRSALTRCLNTYMDRENLFKKEKVAATGDDAREGLTAIVSVKVPDPKF
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCEEEEEEECCCCCC
SSQTKDKLVSSEVKSAVESAMHDKFNDYLQENPSAGKLIAGKIIDAARARDAARKAREMT
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHHH
RRKTTLDIAGLPGKLADCQEKDPALSELYIVEGDSAGGSAKQGRSRKTQAILPLKGKILN
HHHCEEEECCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCEEECCCCCEEEE
VERARFDKMLSSQEVGNLITALGCGIGPDEYNPDKVRYHKIIIMTDADVDGSHIRTLLLT
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHH
FFFRQTPELIERGYIYIAQPPLYKVKKGRQELYLKDDEALKAYLLSSTIDNTRLHISADA
HHHHCCHHHHHCCEEEEECCCCHHHHCCCHHEEECCHHHHHHHHHHHCCCCCEEEEECCC
PAITGQALETLLNDYNQTQLIKARLQIRYPAVLLDALTHTPKLSTDITYDKDAMQAWKDT
CCCCHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHH
LQTQLEHFGSDLNPEIELVNIHAPQPKNMDAAAADLLGMRPVVAVEESDSSDTEAVVAKA
HHHHHHHHCCCCCCCEEEEEEECCCCCCCCHHHHHHHCCCCEEEEECCCCCCHHHHHHHH
QWLPRITVYVHQLAHHYLLDASFINSGEYTKLLRLSAEWNTLLDEDAFIRRDATAGTTKD
HHCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHCCCCHHHCCCCCCCCCCC
IPIRDFDYLWQQMMLDARRGLAIQRYKGLGEMNADQLWDTTMDPENRRMLRVNIEDAIAA
CCCCHHHHHHHHHHHHHHHCCEEHHHCCCCCCCHHHHCCCCCCCCCCEEEEEEHHHHHHH
DHMFTCLMGDHVEPRRIFIEENALTVSNLDI
HHHHHHHHCCCCCCCEEEEECCCEEEECCCC
>Mature Secondary Structure 
SDSLPTDHQQLTSDSAVETIAAAVAPEVLPSDYSSKDIRVLRGLEAVRVRPGMYIGDTD
CCCCCCCHHHHCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCHHEEECCCEEECCCC
DGTGLHHMVFEVVDNSIDEALAGHCDQIDIIIHEDESVSVMDNGRGVPVDIHPEEGVSAA
CCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEECCCCCEEEECCCCCCHHH
QVIMTVLHAGGKFDDNSYKVSGGLHGVGVSVVNALSKKLEMNIWREGYHYHQTYTDGVPD
HHHHHHHHCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCEECCEECCCCCC
GDIQQMEATDQTGTQIRFYPSNDVFTGTIFEYDILAKRLRELSFLNSGVRIVLTDERIDK
CCHHHHHCCCCCCCEEEEEECCCEEEEEHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHH
RHEFEHKGGLSEFVSYINAGKDGINDVFHFISNQDDGISVEVALQWTDTYNEKVFCFTNN
HHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHCCCCCCEEEEEEEEEECCCCCEEEEEECC
IPQRDGGTHLSGFRSALTRCLNTYMDRENLFKKEKVAATGDDAREGLTAIVSVKVPDPKF
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCEEEEEEECCCCCC
SSQTKDKLVSSEVKSAVESAMHDKFNDYLQENPSAGKLIAGKIIDAARARDAARKAREMT
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHHH
RRKTTLDIAGLPGKLADCQEKDPALSELYIVEGDSAGGSAKQGRSRKTQAILPLKGKILN
HHHCEEEECCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCEEECCCCCEEEE
VERARFDKMLSSQEVGNLITALGCGIGPDEYNPDKVRYHKIIIMTDADVDGSHIRTLLLT
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHH
FFFRQTPELIERGYIYIAQPPLYKVKKGRQELYLKDDEALKAYLLSSTIDNTRLHISADA
HHHHCCHHHHHCCEEEEECCCCHHHHCCCHHEEECCHHHHHHHHHHHCCCCCEEEEECCC
PAITGQALETLLNDYNQTQLIKARLQIRYPAVLLDALTHTPKLSTDITYDKDAMQAWKDT
CCCCHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHH
LQTQLEHFGSDLNPEIELVNIHAPQPKNMDAAAADLLGMRPVVAVEESDSSDTEAVVAKA
HHHHHHHHCCCCCCCEEEEEEECCCCCCCCHHHHHHHCCCCEEEEECCCCCCHHHHHHHH
QWLPRITVYVHQLAHHYLLDASFINSGEYTKLLRLSAEWNTLLDEDAFIRRDATAGTTKD
HHCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHCCCCHHHCCCCCCCCCCC
IPIRDFDYLWQQMMLDARRGLAIQRYKGLGEMNADQLWDTTMDPENRRMLRVNIEDAIAA
CCCCHHHHHHHHHHHHHHHCCEEHHHCCCCCCCHHHHCCCCCCCCCCEEEEEEHHHHHHH
DHMFTCLMGDHVEPRRIFIEENALTVSNLDI
HHHHHHHHCCCCCCCEEEEECCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]