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The map label for this gene is rfbB [C]

Identifier: 92113883

GI number: 92113883

Start: 2005021

End: 2006022

Strand: Direct

Name: rfbB [C]

Synonym: Csal_1760

Alternate gene names: 92113883

Gene position: 2005021-2006022 (Clockwise)

Preceding gene: 92113882

Following gene: 92113884

Centisome position: 54.24

GC content: 64.47

Gene sequence:

>1002_bases
ATGAATATCCTGATCACCGGCATGGCCGGCTTCATCGGTCATGCCGTCGCCAAGCGCCTGGCGGCCGAAGGGCATGACAT
CGTCGGCATCGACAACCTCAACGATTACTACGACGTGTCGCTGAAACAGGCCCGCCTGGACGACCTGGCGGCATGGCCGA
ACGTGCGCTTCGAGCGCCTGGACCTGGCCGATCGCGCGGGCATGGAACGCCTGTTCGCCGACACGCGTTTCGAGCGTGTC
ATCCATCTGGCCGCGCAGGCCGGGGTGCGCTATTCCCTCGACAACCCGCACGTCTACGCGCAATCCAACCTGGTGGGTCA
CCTCAATGTGCTGGAGGGCTGCCGCCACCAGCAGGTGCCGCACCTGATCTATGCCTCCTCGAGCTCGGTCTATGGCCAGA
ATGCCCAGGTGCCGTTTTCCACCGCCGATGCCGTGGACCACCCCATCAGCTTGTATGCCGCCACCAAGAAGGCCAACGAG
CTGATGACGCACAGCTACTCGCATTTGTACGGGATTCCCGCCACCGGGCTGCGTTTCTTCACGGTCTACGGCCCCTGGGG
ACGTCCGGACATGGCCATGTTCAAGTTTACCCGGGCGATTCTCGCGGACGAGCCGCTGCCGGTGTTCAACCATGGCGATC
TGTCCCGGGATTTCACCTATATCGACGATATCGTCGAGGGGGTCGTGCGCATCATGCAGGCGATTCCCGAGGCCGACCCG
GACAGTCCGGGCGCGAGTCCCGACCAGAGCACGGCGCCCTTCGCGTTGTACAATATCGGGCATGGCAGTCCGGTGGCCCT
GATGGACTTCGTGCATGCGCTGGAGCGGGCCACGGGACGCACGGCGCGGTGCGACTTCAAGCCCATGCAACCCGGCGACG
TGCCGCGCACCTGGGCGGATACCGACGCGCTCTTCGAGAAGGTCGGCTATCGTCCGCAGGTCGGCGTCGACGAGGGCGTG
GCGCGCTTCGTCGAGTGGTACCGCAGCTTTCACGGGATTTGA

Upstream 100 bases:

>100_bases
CGCATGGCTTGCCCGTCGTCAATGACGCGAGATACATGGATCGGCGCGTTTGCAGCGCCATGCTATAGTCCCGCCATCAC
ACGCAGATGACGCTAGCCTC

Downstream 100 bases:

>100_bases
TGCCCACGATTTTTTGGTTAACGAGCGTAAAGGACGACATACGATGAAGATCAGTATCTTCGGTACGGGATACGTGGGCC
TGGTCACCGGGACTTGCCTG

Product: NAD-dependent epimerase/dehydratase

Products: dTDP-4-dehydro-6-deoxy-D-glucose; H2O [C]

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 333; Mature: 333

Protein sequence:

>333_residues
MNILITGMAGFIGHAVAKRLAAEGHDIVGIDNLNDYYDVSLKQARLDDLAAWPNVRFERLDLADRAGMERLFADTRFERV
IHLAAQAGVRYSLDNPHVYAQSNLVGHLNVLEGCRHQQVPHLIYASSSSVYGQNAQVPFSTADAVDHPISLYAATKKANE
LMTHSYSHLYGIPATGLRFFTVYGPWGRPDMAMFKFTRAILADEPLPVFNHGDLSRDFTYIDDIVEGVVRIMQAIPEADP
DSPGASPDQSTAPFALYNIGHGSPVALMDFVHALERATGRTARCDFKPMQPGDVPRTWADTDALFEKVGYRPQVGVDEGV
ARFVEWYRSFHGI

Sequences:

>Translated_333_residues
MNILITGMAGFIGHAVAKRLAAEGHDIVGIDNLNDYYDVSLKQARLDDLAAWPNVRFERLDLADRAGMERLFADTRFERV
IHLAAQAGVRYSLDNPHVYAQSNLVGHLNVLEGCRHQQVPHLIYASSSSVYGQNAQVPFSTADAVDHPISLYAATKKANE
LMTHSYSHLYGIPATGLRFFTVYGPWGRPDMAMFKFTRAILADEPLPVFNHGDLSRDFTYIDDIVEGVVRIMQAIPEADP
DSPGASPDQSTAPFALYNIGHGSPVALMDFVHALERATGRTARCDFKPMQPGDVPRTWADTDALFEKVGYRPQVGVDEGV
ARFVEWYRSFHGI
>Mature_333_residues
MNILITGMAGFIGHAVAKRLAAEGHDIVGIDNLNDYYDVSLKQARLDDLAAWPNVRFERLDLADRAGMERLFADTRFERV
IHLAAQAGVRYSLDNPHVYAQSNLVGHLNVLEGCRHQQVPHLIYASSSSVYGQNAQVPFSTADAVDHPISLYAATKKANE
LMTHSYSHLYGIPATGLRFFTVYGPWGRPDMAMFKFTRAILADEPLPVFNHGDLSRDFTYIDDIVEGVVRIMQAIPEADP
DSPGASPDQSTAPFALYNIGHGSPVALMDFVHALERATGRTARCDFKPMQPGDVPRTWADTDALFEKVGYRPQVGVDEGV
ARFVEWYRSFHGI

Specific function: DTDP-L-RHAMNOSE BIOSYNTHESIS WITHIN THE O ANTIGEN BIOSYNTHESIS PATHWAY OF LIPOPOLYSACCHARIDE BIOSYNTHESIS. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=340, Percent_Identity=25.2941176470588, Blast_Score=108, Evalue=5e-24,
Organism=Homo sapiens, GI56237023, Length=350, Percent_Identity=26, Blast_Score=108, Evalue=7e-24,
Organism=Homo sapiens, GI56118217, Length=350, Percent_Identity=26, Blast_Score=108, Evalue=7e-24,
Organism=Homo sapiens, GI189083684, Length=350, Percent_Identity=26, Blast_Score=108, Evalue=7e-24,
Organism=Homo sapiens, GI42516563, Length=337, Percent_Identity=25.5192878338279, Blast_Score=101, Evalue=1e-21,
Organism=Escherichia coli, GI1788353, Length=348, Percent_Identity=24.4252873563218, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1786974, Length=345, Percent_Identity=25.7971014492754, Blast_Score=107, Evalue=9e-25,
Organism=Escherichia coli, GI48994969, Length=349, Percent_Identity=26.3610315186246, Blast_Score=104, Evalue=7e-24,
Organism=Escherichia coli, GI1788365, Length=340, Percent_Identity=20.8823529411765, Blast_Score=69, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI71982035, Length=352, Percent_Identity=26.4204545454545, Blast_Score=125, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI71982038, Length=354, Percent_Identity=26.271186440678, Blast_Score=124, Evalue=7e-29,
Organism=Caenorhabditis elegans, GI17568069, Length=344, Percent_Identity=23.8372093023256, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17539532, Length=340, Percent_Identity=23.8235294117647, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI133901786, Length=245, Percent_Identity=26.9387755102041, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17539422, Length=245, Percent_Identity=26.9387755102041, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI133901788, Length=245, Percent_Identity=26.9387755102041, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI133901790, Length=245, Percent_Identity=26.9387755102041, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17539424, Length=245, Percent_Identity=26.9387755102041, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17507723, Length=245, Percent_Identity=27.7551020408163, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6319493, Length=350, Percent_Identity=25.7142857142857, Blast_Score=92, Evalue=8e-20,
Organism=Drosophila melanogaster, GI19923002, Length=346, Percent_Identity=24.8554913294798, Blast_Score=111, Evalue=7e-25,
Organism=Drosophila melanogaster, GI21356223, Length=337, Percent_Identity=22.8486646884273, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040
- InterPro:   IPR008089 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 4.2.1.46 [C]

Molecular weight: Translated: 36936; Mature: 36936

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNILITGMAGFIGHAVAKRLAAEGHDIVGIDNLNDYYDVSLKQARLDDLAAWPNVRFERL
CCEEEECHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHCHHHHHHHHHHHCCCCCCEEHC
DLADRAGMERLFADTRFERVIHLAAQAGVRYSLDNPHVYAQSNLVGHLNVLEGCRHQQVP
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCEEEECCCCEEHHHHHHHHHHCCCC
HLIYASSSSVYGQNAQVPFSTADAVDHPISLYAATKKANELMTHSYSHLYGIPATGLRFF
EEEEECCCCCCCCCCCCCCCCHHHCCCCHHEEEHHHHHHHHHHHHHHHHCCCCCCCEEEE
TVYGPWGRPDMAMFKFTRAILADEPLPVFNHGDLSRDFTYIDDIVEGVVRIMQAIPEADP
EEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCC
DSPGASPDQSTAPFALYNIGHGSPVALMDFVHALERATGRTARCDFKPMQPGDVPRTWAD
CCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCH
TDALFEKVGYRPQVGVDEGVARFVEWYRSFHGI
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNILITGMAGFIGHAVAKRLAAEGHDIVGIDNLNDYYDVSLKQARLDDLAAWPNVRFERL
CCEEEECHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHCHHHHHHHHHHHCCCCCCEEHC
DLADRAGMERLFADTRFERVIHLAAQAGVRYSLDNPHVYAQSNLVGHLNVLEGCRHQQVP
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCEEEECCCCEEHHHHHHHHHHCCCC
HLIYASSSSVYGQNAQVPFSTADAVDHPISLYAATKKANELMTHSYSHLYGIPATGLRFF
EEEEECCCCCCCCCCCCCCCCHHHCCCCHHEEEHHHHHHHHHHHHHHHHCCCCCCCEEEE
TVYGPWGRPDMAMFKFTRAILADEPLPVFNHGDLSRDFTYIDDIVEGVVRIMQAIPEADP
EEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCC
DSPGASPDQSTAPFALYNIGHGSPVALMDFVHALERATGRTARCDFKPMQPGDVPRTWAD
CCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCH
TDALFEKVGYRPQVGVDEGVARFVEWYRSFHGI
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.019 {NAD+}} 0.034 {dTDPglucose}} [C]

Substrates: dTDPglucose [C]

Specific reaction: dTDPglucose --> dTDP-4-dehydro-6-deoxy-D-glucose + H2O [C]

General reaction: Elimination (of H2O C-O bond cleavage [C]

Inhibitor: TDP; TTP [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7682279 [H]