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The map label for this gene is wcaL [H]

Identifier: 92113844

GI number: 92113844

Start: 1959793

End: 1960938

Strand: Direct

Name: wcaL [H]

Synonym: Csal_1721

Alternate gene names: 92113844

Gene position: 1959793-1960938 (Clockwise)

Preceding gene: 92113843

Following gene: 92113845

Centisome position: 53.02

GC content: 66.23

Gene sequence:

>1146_bases
ATGAACGATAAAACGATCGTGCTGGTCTCCAACACGTCCTGGTTTCTTTACAACTTCTGCCAGGGGTTGTTGAGGGCCCT
GGAACGACGCGGCTTCCGGGTGGTGTGCCTGGTACCCCATGACGACTACTCGCAGCGCTTGTGCGAGGAGTTCAACGTCA
CGCTGCGCACCATGCCCATGGACGGCAAGAGCACCGGCCCCGCTCGCGAGGGCAAGTGCCTGTTCTGGCTGTTCGGCCAA
CTGCGCGAGCTGCGCCCTGCCTTCGTCTTCAACTTCACCATCAAGGCCAACATCTATTCGGGGCTGGCCTGCCGCGCGCT
GAACCTTCCCTACGCCAACACCGTGACCGGTCTGGGCACGGCGTTTCTCCACGACAGTCGGCTCTTCCGTCAGGTGCGAC
GCCTGTACGGGGTGGCCAACGCCGGCGCGACACGGGTGTTCTTCCTCAACCCGGATGACCGCGAACTCTTCGAGCACGAG
GGCATGTTGCAGAAGGTCGACTGGGACATGTTGCCCGGTGCGGGCGTCGATGTGGCGCGCTTCGGGTTCAAGCCCTTGCC
CACCGGGGAGCCGTTCACCTTTCTGCTGATCGCCCGGTTGCTGGGCGACAAGGGCGTGCGCGAGTACGTGGCCGCGGCCG
AGCAGGTACGCGCCACGCATCCCGAGACGCGCTTTCTGATCGTCGGCCCCAAGGGCGTCAGCAACCGTACCGCGATCGAG
GACGATGAGGTCGATGCCTGGCACGCGGGCGGGGTCGTCGAGTACGTCGGTGCCCAGGACGACGTGCGGCCCTGGCTCGC
CCAGGCGCATGTGCTGGTACTGCCGTCCTATCGCGAGGGCATGCCGAGCACGGTGATGGAAGCGGCGGCGATGGGGCGAC
CGGCCATCGTGACCGATGTGCCGGGATGCCGGCATGCCGTGACCGAGGGCGAGACCGGCTGGCTGTGCCGCGTCAAGGAC
GCCGAGGCCCTGGCCACGCGCATGCGGTACTGCCTGGCGATGACGCCCTGGGCGCTTTCCCGCGTGGGGGTGGCGGCGCG
CCGGCGTGCGGAGAAGGAGTTCTCGCAGGACATCGTCGTGACGGGATACCTGGCCTGCCTGGAAAGCGGCCTGACGAGTC
ACCAACGCGTGAAAATGGAGGCATGA

Upstream 100 bases:

>100_bases
CGCGTTCCGCGCGGGACAGGGGCCCCGTGTCGCCATTCACGGCTGCCATGTATCCACGACGCATATCCATGACATGTCTC
ACCGCAAGGGAGTTGTTTCC

Downstream 100 bases:

>100_bases
TGACGATTTTAGTGACGGGCGGGGCCGGGTACATCGGCTCGCATATGGTGCTGCGGCTCATCGAAGCCGGCCACGAGGTG
GTCGTGATCGACAACCTCTG

Product: group 1 glycosyl transferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 381; Mature: 381

Protein sequence:

>381_residues
MNDKTIVLVSNTSWFLYNFCQGLLRALERRGFRVVCLVPHDDYSQRLCEEFNVTLRTMPMDGKSTGPAREGKCLFWLFGQ
LRELRPAFVFNFTIKANIYSGLACRALNLPYANTVTGLGTAFLHDSRLFRQVRRLYGVANAGATRVFFLNPDDRELFEHE
GMLQKVDWDMLPGAGVDVARFGFKPLPTGEPFTFLLIARLLGDKGVREYVAAAEQVRATHPETRFLIVGPKGVSNRTAIE
DDEVDAWHAGGVVEYVGAQDDVRPWLAQAHVLVLPSYREGMPSTVMEAAAMGRPAIVTDVPGCRHAVTEGETGWLCRVKD
AEALATRMRYCLAMTPWALSRVGVAARRRAEKEFSQDIVVTGYLACLESGLTSHQRVKMEA

Sequences:

>Translated_381_residues
MNDKTIVLVSNTSWFLYNFCQGLLRALERRGFRVVCLVPHDDYSQRLCEEFNVTLRTMPMDGKSTGPAREGKCLFWLFGQ
LRELRPAFVFNFTIKANIYSGLACRALNLPYANTVTGLGTAFLHDSRLFRQVRRLYGVANAGATRVFFLNPDDRELFEHE
GMLQKVDWDMLPGAGVDVARFGFKPLPTGEPFTFLLIARLLGDKGVREYVAAAEQVRATHPETRFLIVGPKGVSNRTAIE
DDEVDAWHAGGVVEYVGAQDDVRPWLAQAHVLVLPSYREGMPSTVMEAAAMGRPAIVTDVPGCRHAVTEGETGWLCRVKD
AEALATRMRYCLAMTPWALSRVGVAARRRAEKEFSQDIVVTGYLACLESGLTSHQRVKMEA
>Mature_381_residues
MNDKTIVLVSNTSWFLYNFCQGLLRALERRGFRVVCLVPHDDYSQRLCEEFNVTLRTMPMDGKSTGPAREGKCLFWLFGQ
LRELRPAFVFNFTIKANIYSGLACRALNLPYANTVTGLGTAFLHDSRLFRQVRRLYGVANAGATRVFFLNPDDRELFEHE
GMLQKVDWDMLPGAGVDVARFGFKPLPTGEPFTFLLIARLLGDKGVREYVAAAEQVRATHPETRFLIVGPKGVSNRTAIE
DDEVDAWHAGGVVEYVGAQDDVRPWLAQAHVLVLPSYREGMPSTVMEAAAMGRPAIVTDVPGCRHAVTEGETGWLCRVKD
AEALATRMRYCLAMTPWALSRVGVAARRRAEKEFSQDIVVTGYLACLESGLTSHQRVKMEA

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1788357, Length=196, Percent_Identity=31.1224489795918, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296 [H]

Pfam domain/function: PF00534 Glycos_transf_1 [H]

EC number: 2.-.-.- [C]

Molecular weight: Translated: 42541; Mature: 42541

Theoretical pI: Translated: 7.96; Mature: 7.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDKTIVLVSNTSWFLYNFCQGLLRALERRGFRVVCLVPHDDYSQRLCEEFNVTLRTMPM
CCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHCCEEEEECC
DGKSTGPAREGKCLFWLFGQLRELRPAFVFNFTIKANIYSGLACRALNLPYANTVTGLGT
CCCCCCCCCCCCEEEEEHHHHHHCCCEEEEEEEEEEECCCCEEEEEECCCCCHHHHHHHH
AFLHDSRLFRQVRRLYGVANAGATRVFFLNPDDRELFEHEGMLQKVDWDMLPGAGVDVAR
HHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHCCCHHHCCCCCCCCCCCCHHH
FGFKPLPTGEPFTFLLIARLLGDKGVREYVAAAEQVRATHPETRFLIVGPKGVSNRTAIE
CCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCC
DDEVDAWHAGGVVEYVGAQDDVRPWLAQAHVLVLPSYREGMPSTVMEAAAMGRPAIVTDV
CCCCCEEECCCEEEECCCCCCHHHHHHCCEEEEECCHHCCCCHHHHHHHHCCCCEEEECC
PGCRHAVTEGETGWLCRVKDAEALATRMRYCLAMTPWALSRVGVAARRRAEKEFSQDIVV
CCCHHHHCCCCCCCEEEECCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHH
TGYLACLESGLTSHQRVKMEA
HHHHHHHHHCCCCCCEEEECC
>Mature Secondary Structure
MNDKTIVLVSNTSWFLYNFCQGLLRALERRGFRVVCLVPHDDYSQRLCEEFNVTLRTMPM
CCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHCCEEEEECC
DGKSTGPAREGKCLFWLFGQLRELRPAFVFNFTIKANIYSGLACRALNLPYANTVTGLGT
CCCCCCCCCCCCEEEEEHHHHHHCCCEEEEEEEEEEECCCCEEEEEECCCCCHHHHHHHH
AFLHDSRLFRQVRRLYGVANAGATRVFFLNPDDRELFEHEGMLQKVDWDMLPGAGVDVAR
HHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHCCCHHHCCCCCCCCCCCCHHH
FGFKPLPTGEPFTFLLIARLLGDKGVREYVAAAEQVRATHPETRFLIVGPKGVSNRTAIE
CCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCC
DDEVDAWHAGGVVEYVGAQDDVRPWLAQAHVLVLPSYREGMPSTVMEAAAMGRPAIVTDV
CCCCCEEECCCEEEECCCCCCHHHHHHCCEEEEECCHHCCCCHHHHHHHHCCCCEEEECC
PGCRHAVTEGETGWLCRVKDAEALATRMRYCLAMTPWALSRVGVAARRRAEKEFSQDIVV
CCCHHHHCCCCCCCEEEECCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHH
TGYLACLESGLTSHQRVKMEA
HHHHHHHHHCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1710759; 11677609 [H]