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The map label for this gene is rpoN [H]

Identifier: 91790317

GI number: 91790317

Start: 4739321

End: 4740868

Strand: Reverse

Name: rpoN [H]

Synonym: Bpro_4485

Alternate gene names: 91790317

Gene position: 4740868-4739321 (Counterclockwise)

Preceding gene: 91790318

Following gene: 91790316

Centisome position: 91.17

GC content: 63.18

Gene sequence:

>1548_bases
GTGAAGCAAGGGCTTTCTCTCCGCGTCTCGCAGCATCTCGCGCTCACGCCGCAGCTGCAGCAGTCGATTCGCCTGCTGCA
GCTGTCCACGCTTGAGCTGAGCCAGGAAGTCGAGCAGATGCTCGATGAAAATCCGTTTCTGGAGGTGTCTGAAGAAGCCG
CCCCGCGCGAGGAATTTGGCCTGGAACAGACCGACACGCCGGTCAGCCAGGACAGCCGGGAGTTCGAATTTGCTGCAGAT
TCAGTAGCTGTCAATGGCGATGAATCGAGGGCTGCAGGCCAAAATGATGCCGACATTGGCCCCGAGGTCAGTTCCGAGGC
CAAGCTGGAGGAAAGCTGGGAGGGCGACGGCTCGGTGGAGTCCTCGCCTGACGACAGCGAATGGGGCGGCGACGCGCCGG
CCCGCAAGAGCAATGCGGACGACAGCGAGGCCGAGGCTTCCGACCTGGCCCGCAGCCATGAGTCGCTGCAGGACCATTTG
CATCGCCAGTCGCTCGGCCTGCGCCTGTCGGAGGAAGACCAGGCGGCACTGCATTTCCTGATCGAGTCGCTCAATGACGA
CGGCTACCTTGAAGACAGTCTCGCGTCACTGGCCGCTGGCCTGGCGGGCGACGACCTTGAGCAGGCCGAAGAGCTGGAAC
AGCACTTTGCCATGGCCCTGAAGCTGCTGCAGCACATGGAGCCGGCCGGTGTCGGCGCCCGCAACCTGGCCGAGTGCCTC
GGCCTGCAACTGCTCGACTGCAAGGACTGCGACGAGACCCGCGCAGCGATCGCCATGTGCAAGCAGCCCATGGAGCTGCT
GGCCAAGCGGGACGTCAAGCGCCTGTCCTACCTGTGCGGGTTTCCGGACAGCGTGATCAGGGGCGCCATCGCCGTGATCG
GCCGGCTGGATCCCAAGCCCGGGCGGCGGTTTGTCAACGTCGAGCGCAACCTCATCGTGCCCGACGTGATCGTCGTCAAG
TCGGGCCGCGGCTTCAAGGTCTCCCTCAACAGCGAGGTCATGCCCCGCCTGCGCGTGCACGACATCTATGCCAATGCGCT
GAAGCAGCACAAGGGCCAGAACGGGCAGGCCTTGCAGCAGCGCTTGCAGGAAGCGCGCTGGTTCATCAAGAACATCCAGC
AGCGCTTTGACACCATCCTGCGCGTGAGCAGCGCGATTGTGGAGCGGCAAAAGAATTTCTTCACCCACGGCGAGCTGGCC
ATGCGTCCGCTGGTGCTGCGCGAAATTGCCGACGAACTGGGCCTGCACGAGTCCACCATCTCGCGCGTCACCACGGCCAA
GTACATGAGCACGCCGTTCGGCACCTTCGAGCTCAAGTACTTTTTCGGCTCCGCGCTGGGCACCGAGACTGGTGGTAACG
CATCCAGCACCGCGGTGCGCGCACTGATCAAGCAGTTTGTCTCGTCCGAGAGCCTGAAAAAACCGCTCAGCGACAACCAG
ATTTCCGAAATGCTCAAGGAGCAGGGCATTGAATGCGCGCGCCGCACCGTGGCCAAGTACCGCGAAGCTTTGCGCATCGC
GCCTGCCAACCTGCGCAAATCGCTTTGA

Upstream 100 bases:

>100_bases
CCCCTCAAGGGGGCTGCGCCTGCGGCCCGGCAGAGCCGGTTCCGCGGCCCATGCTTGAAAAATCCACCCCGCCCAGCTTT
TGTGGATACCTGGAGGGGTT

Downstream 100 bases:

>100_bases
AAGTAGCCCCCACGGTCGCCCACTGCGTGTGGCTCCCTGCCCCCCGAGGGGGCCGCTGCGCCTGCGGCCCGGCAAAGCCG
GTTCCGCGGCCCCTGCTTGA

Product: sigma-54 (RpoN)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 515; Mature: 515

Protein sequence:

>515_residues
MKQGLSLRVSQHLALTPQLQQSIRLLQLSTLELSQEVEQMLDENPFLEVSEEAAPREEFGLEQTDTPVSQDSREFEFAAD
SVAVNGDESRAAGQNDADIGPEVSSEAKLEESWEGDGSVESSPDDSEWGGDAPARKSNADDSEAEASDLARSHESLQDHL
HRQSLGLRLSEEDQAALHFLIESLNDDGYLEDSLASLAAGLAGDDLEQAEELEQHFAMALKLLQHMEPAGVGARNLAECL
GLQLLDCKDCDETRAAIAMCKQPMELLAKRDVKRLSYLCGFPDSVIRGAIAVIGRLDPKPGRRFVNVERNLIVPDVIVVK
SGRGFKVSLNSEVMPRLRVHDIYANALKQHKGQNGQALQQRLQEARWFIKNIQQRFDTILRVSSAIVERQKNFFTHGELA
MRPLVLREIADELGLHESTISRVTTAKYMSTPFGTFELKYFFGSALGTETGGNASSTAVRALIKQFVSSESLKKPLSDNQ
ISEMLKEQGIECARRTVAKYREALRIAPANLRKSL

Sequences:

>Translated_515_residues
MKQGLSLRVSQHLALTPQLQQSIRLLQLSTLELSQEVEQMLDENPFLEVSEEAAPREEFGLEQTDTPVSQDSREFEFAAD
SVAVNGDESRAAGQNDADIGPEVSSEAKLEESWEGDGSVESSPDDSEWGGDAPARKSNADDSEAEASDLARSHESLQDHL
HRQSLGLRLSEEDQAALHFLIESLNDDGYLEDSLASLAAGLAGDDLEQAEELEQHFAMALKLLQHMEPAGVGARNLAECL
GLQLLDCKDCDETRAAIAMCKQPMELLAKRDVKRLSYLCGFPDSVIRGAIAVIGRLDPKPGRRFVNVERNLIVPDVIVVK
SGRGFKVSLNSEVMPRLRVHDIYANALKQHKGQNGQALQQRLQEARWFIKNIQQRFDTILRVSSAIVERQKNFFTHGELA
MRPLVLREIADELGLHESTISRVTTAKYMSTPFGTFELKYFFGSALGTETGGNASSTAVRALIKQFVSSESLKKPLSDNQ
ISEMLKEQGIECARRTVAKYREALRIAPANLRKSL
>Mature_515_residues
MKQGLSLRVSQHLALTPQLQQSIRLLQLSTLELSQEVEQMLDENPFLEVSEEAAPREEFGLEQTDTPVSQDSREFEFAAD
SVAVNGDESRAAGQNDADIGPEVSSEAKLEESWEGDGSVESSPDDSEWGGDAPARKSNADDSEAEASDLARSHESLQDHL
HRQSLGLRLSEEDQAALHFLIESLNDDGYLEDSLASLAAGLAGDDLEQAEELEQHFAMALKLLQHMEPAGVGARNLAECL
GLQLLDCKDCDETRAAIAMCKQPMELLAKRDVKRLSYLCGFPDSVIRGAIAVIGRLDPKPGRRFVNVERNLIVPDVIVVK
SGRGFKVSLNSEVMPRLRVHDIYANALKQHKGQNGQALQQRLQEARWFIKNIQQRFDTILRVSSAIVERQKNFFTHGELA
MRPLVLREIADELGLHESTISRVTTAKYMSTPFGTFELKYFFGSALGTETGGNASSTAVRALIKQFVSSESLKKPLSDNQ
ISEMLKEQGIECARRTVAKYREALRIAPANLRKSL

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released [H]

COG id: COG1508

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma54 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-54 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789594, Length=517, Percent_Identity=39.0715667311412, Blast_Score=333, Evalue=1e-92,

Paralogues:

None

Copy number: 70 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000394
- InterPro:   IPR007046
- InterPro:   IPR007634 [H]

Pfam domain/function: PF00309 Sigma54_AID; PF04963 Sigma54_CBD; PF04552 Sigma54_DBD [H]

EC number: NA

Molecular weight: Translated: 56986; Mature: 56986

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS00717 SIGMA54_1 ; PS00718 SIGMA54_2 ; PS50044 SIGMA54_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQGLSLRVSQHLALTPQLQQSIRLLQLSTLELSQEVEQMLDENPFLEVSEEAAPREEFG
CCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCHHCCCHHHCC
LEQTDTPVSQDSREFEFAADSVAVNGDESRAAGQNDADIGPEVSSEAKLEESWEGDGSVE
CCCCCCCCCCCCCHHHHHHCCEEECCCHHHCCCCCCCCCCCCCCCCCCHHHCCCCCCCCC
SSPDDSEWGGDAPARKSNADDSEAEASDLARSHESLQDHLHRQSLGLRLSEEDQAALHFL
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
IESLNDDGYLEDSLASLAAGLAGDDLEQAEELEQHFAMALKLLQHMEPAGVGARNLAECL
HHHCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
GLQLLDCKDCDETRAAIAMCKQPMELLAKRDVKRLSYLCGFPDSVIRGAIAVIGRLDPKP
CCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCC
GRRFVNVERNLIVPDVIVVKSGRGFKVSLNSEVMPRLRVHDIYANALKQHKGQNGQALQQ
CCEEEECCCCEECCCEEEEECCCCEEEEECCHHCCHHHHHHHHHHHHHHHCCCCCHHHHH
RLQEARWFIKNIQQRFDTILRVSSAIVERQKNFFTHGELAMRPLVLREIADELGLHESTI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHH
SRVTTAKYMSTPFGTFELKYFFGSALGTETGGNASSTAVRALIKQFVSSESLKKPLSDNQ
HHHHHHHHHCCCCCCEEHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHH
ISEMLKEQGIECARRTVAKYREALRIAPANLRKSL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCC
>Mature Secondary Structure
MKQGLSLRVSQHLALTPQLQQSIRLLQLSTLELSQEVEQMLDENPFLEVSEEAAPREEFG
CCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCHHCCCHHHCC
LEQTDTPVSQDSREFEFAADSVAVNGDESRAAGQNDADIGPEVSSEAKLEESWEGDGSVE
CCCCCCCCCCCCCHHHHHHCCEEECCCHHHCCCCCCCCCCCCCCCCCCHHHCCCCCCCCC
SSPDDSEWGGDAPARKSNADDSEAEASDLARSHESLQDHLHRQSLGLRLSEEDQAALHFL
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
IESLNDDGYLEDSLASLAAGLAGDDLEQAEELEQHFAMALKLLQHMEPAGVGARNLAECL
HHHCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
GLQLLDCKDCDETRAAIAMCKQPMELLAKRDVKRLSYLCGFPDSVIRGAIAVIGRLDPKP
CCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCC
GRRFVNVERNLIVPDVIVVKSGRGFKVSLNSEVMPRLRVHDIYANALKQHKGQNGQALQQ
CCEEEECCCCEECCCEEEEECCCCEEEEECCHHCCHHHHHHHHHHHHHHHCCCCCHHHHH
RLQEARWFIKNIQQRFDTILRVSSAIVERQKNFFTHGELAMRPLVLREIADELGLHESTI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHH
SRVTTAKYMSTPFGTFELKYFFGSALGTETGGNASSTAVRALIKQFVSSESLKKPLSDNQ
HHHHHHHHHCCCCCCEEHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHH
ISEMLKEQGIECARRTVAKYREALRIAPANLRKSL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1417413 [H]