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The map label for this gene is rlmL [H]

Identifier: 91790316

GI number: 91790316

Start: 4737776

End: 4739182

Strand: Reverse

Name: rlmL [H]

Synonym: Bpro_4484

Alternate gene names: 91790316

Gene position: 4739182-4737776 (Counterclockwise)

Preceding gene: 91790317

Following gene: 91790315

Centisome position: 91.13

GC content: 67.16

Gene sequence:

>1407_bases
ATGAACCAACTTCAACTCTTTTTACCTTGTGCCGCCGGCGTCGAGGCCCTGCTGGCCAGCGAGGTCAAACGCATCACCGG
CATCGACGGCAAGGCCTGGCGCGCCGGCGTGCAGCTGCAGGGCTCGTGGCGCGATGCCCTGCAGCTCAACCTGCACAGCC
GCCTGGCCCAGCGCGTGCTGATCGAGCTGCAGCACAACCAGTACCGCAGCGAGCAGGACCTCTACAACGCGGCAGCCAGC
GTCGCCTGGGAAATCTGGTTCACGCCCAAGCAGACCTTCAAGGTCGAGATCACCGCGCAGCACAGCCCACTGACCAGCCT
GAACTTTGCGGCGCTCAAGATCAAGGACGCCATTGCCGACCGCTTCCGTGCCAAGTTTGATGTGCGGCCCGATGTGGACA
CGCGCTGGCCCGACGTGCGCGTGTATGCGCACCTGACGACGGACACGGTGACCATCTATATCGACACCTCGGGCGAGCCG
CTCTTCAAGCGCGGCTGGCGCGAAGACAAGGGCGATGCGCCCCTGAAGGAAACACTGGCCGCCGCCATGATTGCCGCCAG
TGGCTGGGACCAGCAGTGCAAGGAGGGCGTGCCGCTGTACGACCCGTGCTGCGGCTCCGGCACCATCGTGATCGAGGCGG
CGCAGATTGCCTGCAACATCGCGCCCGGCATCAACCGCCGCTTCGCCTTCCAGAAGTATTTGCCCTTCCAGGCCCACGTG
TGGGAGGGGCTGCTCGACCAGGCCGAGGCCGCCATCACGGAGCCGACGGCACCGGTGTTTGGCAGCGATGTGTCGTTCCG
TATGGTGGATTTTGCCGAGCGCAATGCCGAGCGCGCGGGCGTGGCCAACGCCGTGCAACTGCGCGGCGGCGATGCGCTGC
AGCGCATGCCGCCTGCGCCATCGGGTGTGATGCTGGTGAACCCACCGTATGGCGAGCGCATTGACGTGGCCGGCGTCGCA
GGCATCACCGGCGTGCAGGGGCGCGACCAGCGGCGCCAGCAGAACACTGGCATCAGCGGCGCCTGGGACGGCACCCAGGC
CATCGACGAGTTTGGCCAGCCGCTGCCGCGTGATGCATCCGAAGCCGGCCCGGCTGGCAGTTCGCGCGACCGCTCGGCCA
ACCCGGGGCGCGAGCAGGCACAGACAGAAACCGGCGAAGAAGCCTCCGACTTCTTTCCCCGGCTGGCCGCGCACTGGAAG
AAGAACTACGCCGGCTGGACGGCCCATGTGCTCACGCCCGACCTGAAATTGCCCGGCAAGATGCGCCTGAAGGAATCGCG
CCGCGTGCCGATGTGGAACGGCCCGATCGAATGCCGGCTGTTCCGCTTTGACATGGTGGCCGGCTCGGCACGCGGCAAGC
CCGCGTCCGGTTCTACTCCCTCTCCCGCCGGGAGAGGGCCGGGGTGA

Upstream 100 bases:

>100_bases
GCCCCCCGAGGGGGCCGCTGCGCCTGCGGCCCGGCAAAGCCGGTTCCGCGGCCCCTGCTTGAAAAAGAACCGTCACCCAG
CCAGCCGTGAGTCTTCATTC

Downstream 100 bases:

>100_bases
GGGTGGTGCATCACAGGCTTGCCTCCAAGCGCCCCATGGCCTTCAGCGCATGAGCACTCAATCCATCGTCCTGGACACCA
ACATCGTGCTCGATGTGTTT

Product: putative RNA methylase

Products: NA

Alternate protein names: 23S rRNA m2G2445 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase rlmL [H]

Number of amino acids: Translated: 468; Mature: 468

Protein sequence:

>468_residues
MNQLQLFLPCAAGVEALLASEVKRITGIDGKAWRAGVQLQGSWRDALQLNLHSRLAQRVLIELQHNQYRSEQDLYNAAAS
VAWEIWFTPKQTFKVEITAQHSPLTSLNFAALKIKDAIADRFRAKFDVRPDVDTRWPDVRVYAHLTTDTVTIYIDTSGEP
LFKRGWREDKGDAPLKETLAAAMIAASGWDQQCKEGVPLYDPCCGSGTIVIEAAQIACNIAPGINRRFAFQKYLPFQAHV
WEGLLDQAEAAITEPTAPVFGSDVSFRMVDFAERNAERAGVANAVQLRGGDALQRMPPAPSGVMLVNPPYGERIDVAGVA
GITGVQGRDQRRQQNTGISGAWDGTQAIDEFGQPLPRDASEAGPAGSSRDRSANPGREQAQTETGEEASDFFPRLAAHWK
KNYAGWTAHVLTPDLKLPGKMRLKESRRVPMWNGPIECRLFRFDMVAGSARGKPASGSTPSPAGRGPG

Sequences:

>Translated_468_residues
MNQLQLFLPCAAGVEALLASEVKRITGIDGKAWRAGVQLQGSWRDALQLNLHSRLAQRVLIELQHNQYRSEQDLYNAAAS
VAWEIWFTPKQTFKVEITAQHSPLTSLNFAALKIKDAIADRFRAKFDVRPDVDTRWPDVRVYAHLTTDTVTIYIDTSGEP
LFKRGWREDKGDAPLKETLAAAMIAASGWDQQCKEGVPLYDPCCGSGTIVIEAAQIACNIAPGINRRFAFQKYLPFQAHV
WEGLLDQAEAAITEPTAPVFGSDVSFRMVDFAERNAERAGVANAVQLRGGDALQRMPPAPSGVMLVNPPYGERIDVAGVA
GITGVQGRDQRRQQNTGISGAWDGTQAIDEFGQPLPRDASEAGPAGSSRDRSANPGREQAQTETGEEASDFFPRLAAHWK
KNYAGWTAHVLTPDLKLPGKMRLKESRRVPMWNGPIECRLFRFDMVAGSARGKPASGSTPSPAGRGPG
>Mature_468_residues
MNQLQLFLPCAAGVEALLASEVKRITGIDGKAWRAGVQLQGSWRDALQLNLHSRLAQRVLIELQHNQYRSEQDLYNAAAS
VAWEIWFTPKQTFKVEITAQHSPLTSLNFAALKIKDAIADRFRAKFDVRPDVDTRWPDVRVYAHLTTDTVTIYIDTSGEP
LFKRGWREDKGDAPLKETLAAAMIAASGWDQQCKEGVPLYDPCCGSGTIVIEAAQIACNIAPGINRRFAFQKYLPFQAHV
WEGLLDQAEAAITEPTAPVFGSDVSFRMVDFAERNAERAGVANAVQLRGGDALQRMPPAPSGVMLVNPPYGERIDVAGVA
GITGVQGRDQRRQQNTGISGAWDGTQAIDEFGQPLPRDASEAGPAGSSRDRSANPGREQAQTETGEEASDFFPRLAAHWK
KNYAGWTAHVLTPDLKLPGKMRLKESRRVPMWNGPIECRLFRFDMVAGSARGKPASGSTPSPAGRGPG

Specific function: Specifically methylates the guanosine in position 2445 (m2G2445) of 23S rRNA [H]

COG id: COG0116

COG function: function code L; Predicted N6-adenine-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain [H]

Homologues:

Organism=Escherichia coli, GI1787181, Length=337, Percent_Identity=35.3115727002967, Blast_Score=192, Evalue=3e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR000241
- InterPro:   IPR017244
- InterPro:   IPR019614
- InterPro:   IPR004114 [H]

Pfam domain/function: PF10672 Methyltrans_SAM; PF02926 THUMP; PF01170 UPF0020 [H]

EC number: =2.1.1.173 [H]

Molecular weight: Translated: 51203; Mature: 51203

Theoretical pI: Translated: 7.85; Mature: 7.85

Prosite motif: PS51165 THUMP ; PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQLQLFLPCAAGVEALLASEVKRITGIDGKAWRAGVQLQGSWRDALQLNLHSRLAQRVL
CCCEEEEECCHHHHHHHHHHHHHHHHCCCCCCEEECCEEECCCHHHHHHHHHHHHHHHHH
IELQHNQYRSEQDLYNAAASVAWEIWFTPKQTFKVEITAQHSPLTSLNFAALKIKDAIAD
HHHHHHHCCCHHHHHHHHHHEEEEEEECCCCEEEEEEEECCCCCCCCCEEEEHHHHHHHH
RFRAKFDVRPDVDTRWPDVRVYAHLTTDTVTIYIDTSGEPLFKRGWREDKGDAPLKETLA
HHHHEECCCCCCCCCCCCEEEEEEEEECEEEEEEECCCCHHHHCCCCCCCCCCCHHHHHH
AAMIAASGWDQQCKEGVPLYDPCCGSGTIVIEAAQIACNIAPGINRRFAFQKYLPFQAHV
HHHHHHCCCCHHHHCCCCCCCCCCCCCEEEEEEEEEEEECCCCCCCHHHHHHCCCCHHHH
WEGLLDQAEAAITEPTAPVFGSDVSFRMVDFAERNAERAGVANAVQLRGGDALQRMPPAP
HHHHHHHHHHHHCCCCCCCCCCCCCEEEEHHHHHCHHHCCCCCEEEECCCCHHHHCCCCC
SGVMLVNPPYGERIDVAGVAGITGVQGRDQRRQQNTGISGAWDGTQAIDEFGQPLPRDAS
CCEEEECCCCCCEEEECCCCCCCCCCCCHHHHHHCCCCCCCCCCHHHHHHHCCCCCCCCH
EAGPAGSSRDRSANPGREQAQTETGEEASDFFPRLAAHWKKNYAGWTAHVLTPDLKLPGK
HCCCCCCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCC
MRLKESRRVPMWNGPIECRLFRFDMVAGSARGKPASGSTPSPAGRGPG
EEHHHCCCCCCCCCCCEEEEEEEEEEECCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNQLQLFLPCAAGVEALLASEVKRITGIDGKAWRAGVQLQGSWRDALQLNLHSRLAQRVL
CCCEEEEECCHHHHHHHHHHHHHHHHCCCCCCEEECCEEECCCHHHHHHHHHHHHHHHHH
IELQHNQYRSEQDLYNAAASVAWEIWFTPKQTFKVEITAQHSPLTSLNFAALKIKDAIAD
HHHHHHHCCCHHHHHHHHHHEEEEEEECCCCEEEEEEEECCCCCCCCCEEEEHHHHHHHH
RFRAKFDVRPDVDTRWPDVRVYAHLTTDTVTIYIDTSGEPLFKRGWREDKGDAPLKETLA
HHHHEECCCCCCCCCCCCEEEEEEEEECEEEEEEECCCCHHHHCCCCCCCCCCCHHHHHH
AAMIAASGWDQQCKEGVPLYDPCCGSGTIVIEAAQIACNIAPGINRRFAFQKYLPFQAHV
HHHHHHCCCCHHHHCCCCCCCCCCCCCEEEEEEEEEEEECCCCCCCHHHHHHCCCCHHHH
WEGLLDQAEAAITEPTAPVFGSDVSFRMVDFAERNAERAGVANAVQLRGGDALQRMPPAP
HHHHHHHHHHHHCCCCCCCCCCCCCEEEEHHHHHCHHHCCCCCEEEECCCCHHHHCCCCC
SGVMLVNPPYGERIDVAGVAGITGVQGRDQRRQQNTGISGAWDGTQAIDEFGQPLPRDAS
CCEEEECCCCCCEEEECCCCCCCCCCCCHHHHHHCCCCCCCCCCHHHHHHHCCCCCCCCH
EAGPAGSSRDRSANPGREQAQTETGEEASDFFPRLAAHWKKNYAGWTAHVLTPDLKLPGK
HCCCCCCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCC
MRLKESRRVPMWNGPIECRLFRFDMVAGSARGKPASGSTPSPAGRGPG
EEHHHCCCCCCCCCCCEEEEEEEEEEECCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA