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The map label for this gene is tilS [H]

Identifier: 91788722

GI number: 91788722

Start: 3016674

End: 3017654

Strand: Reverse

Name: tilS [H]

Synonym: Bpro_2861

Alternate gene names: 91788722

Gene position: 3017654-3016674 (Counterclockwise)

Preceding gene: 91788723

Following gene: 91788721

Centisome position: 58.03

GC content: 68.2

Gene sequence:

>981_bases
GTGGCTCGTAGCGCGACGGCGCAAGGCTTACCCGAAGCGCTGGCTTCTTTCTCTCCGGGCCTGCCTTTCGCTGTAGCGCT
CAGCGGCGGCGCCGATTCAACCGCCTTGCTGGTGGGCTGCGCCGAGAAATGGCCTGGTCAGGTCCGGGCCGTTCATATCC
ATCACGGCCTGCAGGCTGCAGCCGACGACTTTGAACGGCACTGCCAGGCCCTGTGCGCGCAGTTGAACGTGCCGCTTGCC
GTGAAACGCGTGCAGGCCCGCCATGCACCCGGGGAAAGTCCCGAGGACGCGGCCCGCAAGGCCCGTTATGCGGCTTTTGG
GGCTGTTGCTCGTGAAGAATGGGCGCAGCATGCCATCAAAGACATAGCAATAGCCCAGCATGCCGATGACCAGGTTGAAA
CCATGCTGCTGGCGCTGTCGCGCGGGGCAGGGCTGCCGGGGCTGGCCGCCATGCCGGCGCGGTGGGAGCGGGACGGCATC
GTCTACCACCGCCCGCTGCTGGCGGTGCCCGGGGCGCTGGTGCGAGCCTGGCTGGTCGAACGGGGCTTGCCCTGGGTGGA
AGATCCGACCAATACCGATGAGCGGTTCACGCGTAACCGCATTCGGGCCCGGCTGTTGCCCGCGCTGGCGGACGCCTTTC
CGCAGTTCCGCGAGACCTTTGCGCGCAGTGCCCGACACGCGGCCCAGGCCCAGGCGCTGCTGGTGGAGGTGGCGCAGCAG
GATCTGGCCACGGTCGGCCTGCCGCCTGCCATCACGGCCCTGCAGGGTTTGTCGCAGCCACGCCAGGCCAATGTGCTGCG
CCATTGGCTGCAGCTGCAACAGGCCACACCCAGCGCGGCGCAACTCGATCAACTGCTGACGCAAGTGGCCGCCTGCACCA
CCCGCGGGCACCAGATCCACCTTAAGGTGGCGACAGGCCACGTGACGCGCGTCGGCACGGTGCTGCACTACACCGCCGGG
GCGGGTCAGACGGACGATTAG

Upstream 100 bases:

>100_bases
GACGGCGAGACCGGCGTCCCGGTCAGCTTTGCTACGCGGCCTTTGTGGCTGCTCCGGGGCGAGGACCGGACAGGGCGCAA
TGATTTCTCGGTGGGCCACC

Downstream 100 bases:

>100_bases
GTAATAGCCCTAGGTATAATTAAAGGCTTTGCTGGCAGCCAAAGCCTTCCCTGCAGAATGCAGAAAGGGCATGAGAGGCC
TGCAAAAAGCAGCACGCAAT

Product: tRNA(Ile)-lysidine synthetase-like protein

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 326; Mature: 325

Protein sequence:

>326_residues
MARSATAQGLPEALASFSPGLPFAVALSGGADSTALLVGCAEKWPGQVRAVHIHHGLQAAADDFERHCQALCAQLNVPLA
VKRVQARHAPGESPEDAARKARYAAFGAVAREEWAQHAIKDIAIAQHADDQVETMLLALSRGAGLPGLAAMPARWERDGI
VYHRPLLAVPGALVRAWLVERGLPWVEDPTNTDERFTRNRIRARLLPALADAFPQFRETFARSARHAAQAQALLVEVAQQ
DLATVGLPPAITALQGLSQPRQANVLRHWLQLQQATPSAAQLDQLLTQVAACTTRGHQIHLKVATGHVTRVGTVLHYTAG
AGQTDD

Sequences:

>Translated_326_residues
MARSATAQGLPEALASFSPGLPFAVALSGGADSTALLVGCAEKWPGQVRAVHIHHGLQAAADDFERHCQALCAQLNVPLA
VKRVQARHAPGESPEDAARKARYAAFGAVAREEWAQHAIKDIAIAQHADDQVETMLLALSRGAGLPGLAAMPARWERDGI
VYHRPLLAVPGALVRAWLVERGLPWVEDPTNTDERFTRNRIRARLLPALADAFPQFRETFARSARHAAQAQALLVEVAQQ
DLATVGLPPAITALQGLSQPRQANVLRHWLQLQQATPSAAQLDQLLTQVAACTTRGHQIHLKVATGHVTRVGTVLHYTAG
AGQTDD
>Mature_325_residues
ARSATAQGLPEALASFSPGLPFAVALSGGADSTALLVGCAEKWPGQVRAVHIHHGLQAAADDFERHCQALCAQLNVPLAV
KRVQARHAPGESPEDAARKARYAAFGAVAREEWAQHAIKDIAIAQHADDQVETMLLALSRGAGLPGLAAMPARWERDGIV
YHRPLLAVPGALVRAWLVERGLPWVEDPTNTDERFTRNRIRARLLPALADAFPQFRETFARSARHAAQAQALLVEVAQQD
LATVGLPPAITALQGLSQPRQANVLRHWLQLQQATPSAAQLDQLLTQVAACTTRGHQIHLKVATGHVTRVGTVLHYTAGA
GQTDD

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=274, Percent_Identity=40.5109489051095, Blast_Score=144, Evalue=7e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012795
- InterPro:   IPR015262
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3; PF09179 DUF1946 [H]

EC number: NA

Molecular weight: Translated: 34994; Mature: 34863

Theoretical pI: Translated: 8.38; Mature: 8.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARSATAQGLPEALASFSPGLPFAVALSGGADSTALLVGCAEKWPGQVRAVHIHHGLQAA
CCCCCCCCCHHHHHHHCCCCCCEEEEECCCCCCCEEEEECHHHCCCCEEEEEEHHCHHHH
ADDFERHCQALCAQLNVPLAVKRVQARHAPGESPEDAARKARYAAFGAVAREEWAQHAIK
HHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
DIAIAQHADDQVETMLLALSRGAGLPGLAAMPARWERDGIVYHRPLLAVPGALVRAWLVE
HHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEECHHHHCCHHHHHHHHHH
RGLPWVEDPTNTDERFTRNRIRARLLPALADAFPQFRETFARSARHAAQAQALLVEVAQQ
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DLATVGLPPAITALQGLSQPRQANVLRHWLQLQQATPSAAQLDQLLTQVAACTTRGHQIH
HHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEE
LKVATGHVTRVGTVLHYTAGAGQTDD
EEEECCCHHHHHHHHEEECCCCCCCC
>Mature Secondary Structure 
ARSATAQGLPEALASFSPGLPFAVALSGGADSTALLVGCAEKWPGQVRAVHIHHGLQAA
CCCCCCCCHHHHHHHCCCCCCEEEEECCCCCCCEEEEECHHHCCCCEEEEEEHHCHHHH
ADDFERHCQALCAQLNVPLAVKRVQARHAPGESPEDAARKARYAAFGAVAREEWAQHAIK
HHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
DIAIAQHADDQVETMLLALSRGAGLPGLAAMPARWERDGIVYHRPLLAVPGALVRAWLVE
HHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEECHHHHCCHHHHHHHHHH
RGLPWVEDPTNTDERFTRNRIRARLLPALADAFPQFRETFARSARHAAQAQALLVEVAQQ
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DLATVGLPPAITALQGLSQPRQANVLRHWLQLQQATPSAAQLDQLLTQVAACTTRGHQIH
HHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEE
LKVATGHVTRVGTVLHYTAGAGQTDD
EEEECCCHHHHHHHHEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA