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The map label for this gene is rnhA

Identifier: 91788150

GI number: 91788150

Start: 2371565

End: 2372044

Strand: Direct

Name: rnhA

Synonym: Bpro_2281

Alternate gene names: 91788150

Gene position: 2371565-2372044 (Clockwise)

Preceding gene: 91788149

Following gene: 91788151

Centisome position: 45.6

GC content: 62.71

Gene sequence:

>480_bases
ATGACCGACACACAAGCAGGCACAACAACACAGACACAGGTGGTGATTTACACCGATGGCGCATGCAAGGGTAATCCCGG
GCCCGGTGGCTGGGGGGTATTGCTGGCGATGGGCGATACCGAAAAAGAGCTGTTTGGCGGTGAACCCGTCACCACCAACA
ACCGCATGGAAATGACCGCTGTGATCGAGGCGCTGGCCGCACTCAAACGGCCCTGCCGTGTCACGCTGTACCTGGACAGC
GAGTACGTCCGCAAGGGCATCACCGAGTGGATCCACGGCTGGAAGGCCAGGGGCTGGCGTACCGCCGCCAAGGCACCCGT
CAAAAACGTGGATCTGTGGCAACGGCTGGATGCACTGGTGACTTCCAGCGGCCACAAAATCGATTGGCGATGGGTCAAGG
GCCACAATGGCGACCCCGGCAACGAGCGCGCCGATGCGCTGGCCAACCAGGGTGTCGAGCGCGCGCTGGGCCGTCGCTGA

Upstream 100 bases:

>100_bases
GTGGCCAACAAGCAGCGGGCAACGCGCTCCGGGCCGCAGGCCTGACTTTTTCAACCCCCGGGGCCAGACCTTCGCGTCTC
CCCGGAATTCACGACAACCC

Downstream 100 bases:

>100_bases
CTTCCTTGAACTGCCACGGATCATCCTGGGCATGAGAGGAAAAAGGAAGAATTCGCACCATAAGTAAGCGTACATAGCAT
TGTCGCAAGCGCGGTGCTGC

Product: ribonuclease H

Products: NA

Alternate protein names: RNase H

Number of amino acids: Translated: 159; Mature: 158

Protein sequence:

>159_residues
MTDTQAGTTTQTQVVIYTDGACKGNPGPGGWGVLLAMGDTEKELFGGEPVTTNNRMEMTAVIEALAALKRPCRVTLYLDS
EYVRKGITEWIHGWKARGWRTAAKAPVKNVDLWQRLDALVTSSGHKIDWRWVKGHNGDPGNERADALANQGVERALGRR

Sequences:

>Translated_159_residues
MTDTQAGTTTQTQVVIYTDGACKGNPGPGGWGVLLAMGDTEKELFGGEPVTTNNRMEMTAVIEALAALKRPCRVTLYLDS
EYVRKGITEWIHGWKARGWRTAAKAPVKNVDLWQRLDALVTSSGHKIDWRWVKGHNGDPGNERADALANQGVERALGRR
>Mature_158_residues
TDTQAGTTTQTQVVIYTDGACKGNPGPGGWGVLLAMGDTEKELFGGEPVTTNNRMEMTAVIEALAALKRPCRVTLYLDSE
YVRKGITEWIHGWKARGWRTAAKAPVKNVDLWQRLDALVTSSGHKIDWRWVKGHNGDPGNERADALANQGVERALGRR

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0328

COG function: function code L; Ribonuclease HI

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RNase H domain

Homologues:

Organism=Homo sapiens, GI21359816, Length=147, Percent_Identity=36.0544217687075, Blast_Score=77, Evalue=6e-15,
Organism=Escherichia coli, GI1786408, Length=136, Percent_Identity=61.7647058823529, Blast_Score=171, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI115532542, Length=152, Percent_Identity=39.4736842105263, Blast_Score=92, Evalue=9e-20,
Organism=Caenorhabditis elegans, GI17505482, Length=130, Percent_Identity=33.8461538461538, Blast_Score=67, Evalue=4e-12,
Organism=Drosophila melanogaster, GI45552509, Length=149, Percent_Identity=36.9127516778523, Blast_Score=90, Evalue=6e-19,
Organism=Drosophila melanogaster, GI17137476, Length=149, Percent_Identity=36.9127516778523, Blast_Score=90, Evalue=6e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH_POLSJ (Q12B88)

Other databases:

- EMBL:   CP000316
- RefSeq:   YP_549102.1
- ProteinModelPortal:   Q12B88
- SMR:   Q12B88
- STRING:   Q12B88
- GeneID:   4015048
- GenomeReviews:   CP000316_GR
- KEGG:   pol:Bpro_2281
- eggNOG:   COG0328
- HOGENOM:   HBG742437
- OMA:   NGWRTAD
- PhylomeDB:   Q12B88
- ProtClustDB:   PRK00203
- BioCyc:   PSP296591:BPRO_2281-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00042
- InterPro:   IPR022892
- InterPro:   IPR012337
- InterPro:   IPR002156

Pfam domain/function: PF00075 RnaseH; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 17461; Mature: 17330

Theoretical pI: Translated: 9.19; Mature: 9.19

Prosite motif: PS50879 RNASE_H

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDTQAGTTTQTQVVIYTDGACKGNPGPGGWGVLLAMGDTEKELFGGEPVTTNNRMEMTA
CCCCCCCCCCCEEEEEEECCCCCCCCCCCCEEEEEEECCCHHHHCCCCCCCCCCCHHHHH
VIEALAALKRPCRVTLYLDSEYVRKGITEWIHGWKARGWRTAAKAPVKNVDLWQRLDALV
HHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHCCCCCCCHHCCCCCCCHHHHHHHHHH
TSSGHKIDWRWVKGHNGDPGNERADALANQGVERALGRR
CCCCCEEEEEEECCCCCCCCCHHHHHHHHCCHHHHHCCC
>Mature Secondary Structure 
TDTQAGTTTQTQVVIYTDGACKGNPGPGGWGVLLAMGDTEKELFGGEPVTTNNRMEMTA
CCCCCCCCCCEEEEEEECCCCCCCCCCCCEEEEEEECCCHHHHCCCCCCCCCCCHHHHH
VIEALAALKRPCRVTLYLDSEYVRKGITEWIHGWKARGWRTAAKAPVKNVDLWQRLDALV
HHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHCCCCCCCHHCCCCCCCHHHHHHHHHH
TSSGHKIDWRWVKGHNGDPGNERADALANQGVERALGRR
CCCCCEEEEEEECCCCCCCCCHHHHHHHHCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA