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The map label for this gene is pleD [H]

Identifier: 91787249

GI number: 91787249

Start: 1393767

End: 1394816

Strand: Direct

Name: pleD [H]

Synonym: Bpro_1354

Alternate gene names: 91787249

Gene position: 1393767-1394816 (Clockwise)

Preceding gene: 91787248

Following gene: 91787250

Centisome position: 26.8

GC content: 67.9

Gene sequence:

>1050_bases
ATGAGCAGCGTGCTTGATTCCACCCGCGCTATGGACCGCAAGCTGGACCACACCCTGGACCGCGCCAACAGTGACGTGGT
GCTGATCGTGGACGACGTGCCGGACAACCTCTCGGTGCTGCATGATGCGCTCGATGAGTCGGGTTACACCGTGCTGGTCG
CCACCAGCGGCGAAGCCGCCCTGAACCGGGCCCGGCAGGCCGTGCCGGACATCGTGCTGCTCGACGCCATGATGCCTGGC
ATGGACGGCTTCGAGGTGGCCAAACGCCTGAAAGCCATGGCCGAGACGGCGCACATTCCCATCATCTTCATGACCGGGCT
GACCGAGACCGAGCATCTGGTCGCTGCGCTGGAGGCAGGCGGCGTCGATTACGTGACCAAGCCGATCAAGCCCAAGGAGG
TCATGGCGCGCATGGGGGTGCACCTGCAGGGCGCTCGCCGTGCCCGGCAGGAGGCCCGGCAGGCGGGCCAGGCGCGCAAT
GCGCTGGATGCGTTCGGCTATGCCAGCATCACGGTGCGCATGGGCGGGCCGGGCGACGGCAGGCTGATCTGGCAGACCCC
GCTGGCGCGCGACCTGCTGATGCGCTACTACGGCACGTCGGCCCCGCAAACCCCCGAACCGGTGCTGAACTGGCTGCGCC
GCCACCAGGCCGAGGCCGGGCGCGAGCACGAGCTTGAACCGCCGCGCCTGGCCATGGAACTGGGCCCGCGCCGCCTGACG
TTCCGGCTGCACCGGCACACCGGGGACGCGGGAGGAGACGGTGAGGGCGCGAGCGACTGGCTGATCGTGATGCGCGAGAT
CTCCGACGATGCGGTGATCGAGGCCATGAGCCTGAGCTTCAAGCTCACGGCCCGCGAGGCCGAGGTGCTCTACTGGGTGA
TCAAGGGCAAGATCAACCGCGACATCGCCGGCATTCTGGGTGCCAGTCCGGCCACGGTCAAAAAGCATCTGGAGCATGTG
TACGCCAAGCTGGGCGTGGAAACCCGCACGGCCGCCGCCGGCATGGCGCTGAACCGCATCCGGCAGCTTCATCCGCAGTT
TGAGGCCTGA

Upstream 100 bases:

>100_bases
CAACCCGCTACCGCCGCCTTCGTCGAGGACATGCGGGCGCTGGCGCGGCAGTTCCAGTTCGAAGCCATGGGCCGGCAGCT
CACCAACCAGGAGGCCGCCC

Downstream 100 bases:

>100_bases
GAGGCTGAGAGTCTTTTGTTCATACCCGCTTATCACGCCAAAACGCACCCACTCGTTGTTGCAAATGCGCGCCATAGCCC
GAGCTATGGCTGTGCTTTGC

Product: two component LuxR family transcriptional regulator

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 349; Mature: 348

Protein sequence:

>349_residues
MSSVLDSTRAMDRKLDHTLDRANSDVVLIVDDVPDNLSVLHDALDESGYTVLVATSGEAALNRARQAVPDIVLLDAMMPG
MDGFEVAKRLKAMAETAHIPIIFMTGLTETEHLVAALEAGGVDYVTKPIKPKEVMARMGVHLQGARRARQEARQAGQARN
ALDAFGYASITVRMGGPGDGRLIWQTPLARDLLMRYYGTSAPQTPEPVLNWLRRHQAEAGREHELEPPRLAMELGPRRLT
FRLHRHTGDAGGDGEGASDWLIVMREISDDAVIEAMSLSFKLTAREAEVLYWVIKGKINRDIAGILGASPATVKKHLEHV
YAKLGVETRTAAAGMALNRIRQLHPQFEA

Sequences:

>Translated_349_residues
MSSVLDSTRAMDRKLDHTLDRANSDVVLIVDDVPDNLSVLHDALDESGYTVLVATSGEAALNRARQAVPDIVLLDAMMPG
MDGFEVAKRLKAMAETAHIPIIFMTGLTETEHLVAALEAGGVDYVTKPIKPKEVMARMGVHLQGARRARQEARQAGQARN
ALDAFGYASITVRMGGPGDGRLIWQTPLARDLLMRYYGTSAPQTPEPVLNWLRRHQAEAGREHELEPPRLAMELGPRRLT
FRLHRHTGDAGGDGEGASDWLIVMREISDDAVIEAMSLSFKLTAREAEVLYWVIKGKINRDIAGILGASPATVKKHLEHV
YAKLGVETRTAAAGMALNRIRQLHPQFEA
>Mature_348_residues
SSVLDSTRAMDRKLDHTLDRANSDVVLIVDDVPDNLSVLHDALDESGYTVLVATSGEAALNRARQAVPDIVLLDAMMPGM
DGFEVAKRLKAMAETAHIPIIFMTGLTETEHLVAALEAGGVDYVTKPIKPKEVMARMGVHLQGARRARQEARQAGQARNA
LDAFGYASITVRMGGPGDGRLIWQTPLARDLLMRYYGTSAPQTPEPVLNWLRRHQAEAGREHELEPPRLAMELGPRRLTF
RLHRHTGDAGGDGEGASDWLIVMREISDDAVIEAMSLSFKLTAREAEVLYWVIKGKINRDIAGILGASPATVKKHLEHVY
AKLGVETRTAAAGMALNRIRQLHPQFEA

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1788394, Length=141, Percent_Identity=31.9148936170213, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1786599, Length=112, Percent_Identity=35.7142857142857, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1790860, Length=123, Percent_Identity=33.3333333333333, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1790437, Length=112, Percent_Identity=36.6071428571429, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1788550, Length=131, Percent_Identity=32.824427480916, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1789809, Length=146, Percent_Identity=30.1369863013699, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI145693157, Length=134, Percent_Identity=32.089552238806, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1786784, Length=112, Percent_Identity=32.1428571428571, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI87082012, Length=117, Percent_Identity=28.2051282051282, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1790299, Length=111, Percent_Identity=33.3333333333333, Blast_Score=63, Evalue=4e-11,
Organism=Escherichia coli, GI1787229, Length=170, Percent_Identity=28.8235294117647, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 38288; Mature: 38157

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSVLDSTRAMDRKLDHTLDRANSDVVLIVDDVPDNLSVLHDALDESGYTVLVATSGEAA
CCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCCCEEEEECCCHHH
LNRARQAVPDIVLLDAMMPGMDGFEVAKRLKAMAETAHIPIIFMTGLTETEHLVAALEAG
HHHHHHHCCCCEEHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCC
GVDYVTKPIKPKEVMARMGVHLQGARRARQEARQAGQARNALDAFGYASITVRMGGPGDG
CCCHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCC
RLIWQTPLARDLLMRYYGTSAPQTPEPVLNWLRRHQAEAGREHELEPPRLAMELGPRRLT
EEEECCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCEEE
FRLHRHTGDAGGDGEGASDWLIVMREISDDAVIEAMSLSFKLTAREAEVLYWVIKGKINR
EEEEECCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHEEEEEECCCEEEEEEEECCCCC
DIAGILGASPATVKKHLEHVYAKLGVETRTAAAGMALNRIRQLHPQFEA
CHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SSVLDSTRAMDRKLDHTLDRANSDVVLIVDDVPDNLSVLHDALDESGYTVLVATSGEAA
CCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCCCEEEEECCCHHH
LNRARQAVPDIVLLDAMMPGMDGFEVAKRLKAMAETAHIPIIFMTGLTETEHLVAALEAG
HHHHHHHCCCCEEHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCC
GVDYVTKPIKPKEVMARMGVHLQGARRARQEARQAGQARNALDAFGYASITVRMGGPGDG
CCCHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCC
RLIWQTPLARDLLMRYYGTSAPQTPEPVLNWLRRHQAEAGREHELEPPRLAMELGPRRLT
EEEECCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCEEE
FRLHRHTGDAGGDGEGASDWLIVMREISDDAVIEAMSLSFKLTAREAEVLYWVIKGKINR
EEEEECCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHEEEEEECCCEEEEEEEECCCCC
DIAGILGASPATVKKHLEHVYAKLGVETRTAAAGMALNRIRQLHPQFEA
CHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA