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The map label for this gene is pyrB [H]

Identifier: 91787042

GI number: 91787042

Start: 1187280

End: 1188242

Strand: Direct

Name: pyrB [H]

Synonym: Bpro_1145

Alternate gene names: 91787042

Gene position: 1187280-1188242 (Clockwise)

Preceding gene: 91787041

Following gene: 91787043

Centisome position: 22.83

GC content: 60.54

Gene sequence:

>963_bases
GTGTTGTACCGACGAAACCCACAACTCAATAAAAATGGTGAACTGATTCATCTGCTGTCGATCGAGGGCTTGCCCAAGGC
CATCCTGACGCAGATTCTGGACACGGCCGCCAACTTTGTCAGCGTGAACAACCGCGAGGTCAAAAAAGTGCCGCTGCTGC
GCGGTAAGAGCGTTTTTAACCTGTTTTTTGAAAACTCCACCCGCACCCGAACCACCTTTGAAATTGCCGCCACGCGCCTG
TCGGCAGACGTGATCAACCTCGATATCGCGCGTTCGTCGGCCTCCAAGGGCGAGTCGCTGCTGGACACCATTGCCAACCT
GAGCGCCATGGCCGCCGACCTGTTTGTGGTGCGCCACGGCGAATCCGGTGCGCCCTACCTGATCGCCCAGCATGTCGCTC
CGCACGTGCATGTGATCAATGCCGGCGATGGCCGCCATGCCCACCCGACGCAGGGCCTGCTCGACATGTACACCATCCGC
CACTACAAGAAGGACTTCAGCAACCTGACGGTGGCCATCGTCGGCGACGTGCTGCATTCGCGCGTGGCGCGCTCTGATAT
CCATGCGCTCACCACGCTGGGTTGCCCCGAGGTCCGCGTGGTGGGCCCCAAAACGCTGGTGCCCGCCGACATGGCGCAGA
TGGGCGTGCGCGTCTGCAACACGCTGGAAGAGGGTATCAAGGGCGCTGACGTGATCATCATGCTCAGGCTGCAAAACGAA
CGCATGTCGGGCGCCTTGCTGCCCAGCAGCCAGGAGTTCTTCAAGAGCTTCGGCCTGACCAACGAGAAGCTGCAACTGGC
CAAACCCGATGCCATCGTCATGCACCCGGGGCCGATCAACCGGGGTGTTGAAATTGACTCGGCGGTGGTCGATGGCACGC
AGAGCGTCATCCTGCCGCAGGTCACGTTCGGTATTGCCGTGCGCATGGCTGTCATGGGCATCGTTGCAGGGAATGAAGCA
TGA

Upstream 100 bases:

>100_bases
TCGCGCGCGCGGACAACGGCCAGCTCAGTTTTCAGGTCCAGGACCGCTAAGCCAGAGAAGAACCCCAGCAAAAATCCAGA
GCGCAAGAGGAGCCCAGAGC

Downstream 100 bases:

>100_bases
ATATGGCCCCCACGCTTGTCACTTCGTGTACTGCGCTGCCCCCCAAGGGGGTTGAACTTCGCTTGGGGCGGCCCGGCGCT
TCGTTCGTGACCCCCTCGCT

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase [H]

Number of amino acids: Translated: 320; Mature: 320

Protein sequence:

>320_residues
MLYRRNPQLNKNGELIHLLSIEGLPKAILTQILDTAANFVSVNNREVKKVPLLRGKSVFNLFFENSTRTRTTFEIAATRL
SADVINLDIARSSASKGESLLDTIANLSAMAADLFVVRHGESGAPYLIAQHVAPHVHVINAGDGRHAHPTQGLLDMYTIR
HYKKDFSNLTVAIVGDVLHSRVARSDIHALTTLGCPEVRVVGPKTLVPADMAQMGVRVCNTLEEGIKGADVIIMLRLQNE
RMSGALLPSSQEFFKSFGLTNEKLQLAKPDAIVMHPGPINRGVEIDSAVVDGTQSVILPQVTFGIAVRMAVMGIVAGNEA

Sequences:

>Translated_320_residues
MLYRRNPQLNKNGELIHLLSIEGLPKAILTQILDTAANFVSVNNREVKKVPLLRGKSVFNLFFENSTRTRTTFEIAATRL
SADVINLDIARSSASKGESLLDTIANLSAMAADLFVVRHGESGAPYLIAQHVAPHVHVINAGDGRHAHPTQGLLDMYTIR
HYKKDFSNLTVAIVGDVLHSRVARSDIHALTTLGCPEVRVVGPKTLVPADMAQMGVRVCNTLEEGIKGADVIIMLRLQNE
RMSGALLPSSQEFFKSFGLTNEKLQLAKPDAIVMHPGPINRGVEIDSAVVDGTQSVILPQVTFGIAVRMAVMGIVAGNEA
>Mature_320_residues
MLYRRNPQLNKNGELIHLLSIEGLPKAILTQILDTAANFVSVNNREVKKVPLLRGKSVFNLFFENSTRTRTTFEIAATRL
SADVINLDIARSSASKGESLLDTIANLSAMAADLFVVRHGESGAPYLIAQHVAPHVHVINAGDGRHAHPTQGLLDMYTIR
HYKKDFSNLTVAIVGDVLHSRVARSDIHALTTLGCPEVRVVGPKTLVPADMAQMGVRVCNTLEEGIKGADVIIMLRLQNE
RMSGALLPSSQEFFKSFGLTNEKLQLAKPDAIVMHPGPINRGVEIDSAVVDGTQSVILPQVTFGIAVRMAVMGIVAGNEA

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=311, Percent_Identity=34.7266881028939, Blast_Score=151, Evalue=9e-37,
Organism=Escherichia coli, GI2367364, Length=309, Percent_Identity=34.6278317152104, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI1786469, Length=232, Percent_Identity=30.1724137931034, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1790703, Length=230, Percent_Identity=26.9565217391304, Blast_Score=62, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI193204318, Length=335, Percent_Identity=31.044776119403, Blast_Score=114, Evalue=9e-26,
Organism=Saccharomyces cerevisiae, GI6322331, Length=311, Percent_Identity=30.5466237942122, Blast_Score=122, Evalue=5e-29,
Organism=Drosophila melanogaster, GI24642586, Length=313, Percent_Identity=33.8658146964856, Blast_Score=131, Evalue=7e-31,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.2 [H]

Molecular weight: Translated: 34682; Mature: 34682

Theoretical pI: Translated: 8.71; Mature: 8.71

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLYRRNPQLNKNGELIHLLSIEGLPKAILTQILDTAANFVSVNNREVKKVPLLRGKSVFN
CCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHEECCCCCEEECCCCCCCCEEE
LFFENSTRTRTTFEIAATRLSADVINLDIARSSASKGESLLDTIANLSAMAADLFVVRHG
EEECCCCCCEEEHHHHHHHHHHHEEEEEEECCCCCCCHHHHHHHHHHHHHHHEEEEEEEC
ESGAPYLIAQHVAPHVHVINAGDGRHAHPTQGLLDMYTIRHYKKDFSNLTVAIVGDVLHS
CCCCCEEEEECCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCEEHHHHHHHHHH
RVARSDIHALTTLGCPEVRVVGPKTLVPADMAQMGVRVCNTLEEGIKGADVIIMLRLQNE
HHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCC
RMSGALLPSSQEFFKSFGLTNEKLQLAKPDAIVMHPGPINRGVEIDSAVVDGTQSVILPQ
CCCCCCCCCHHHHHHHHCCCCCCEEEECCCEEEECCCCCCCCCEECHHHHCCCCCEEHHH
VTFGIAVRMAVMGIVAGNEA
HHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MLYRRNPQLNKNGELIHLLSIEGLPKAILTQILDTAANFVSVNNREVKKVPLLRGKSVFN
CCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHEECCCCCEEECCCCCCCCEEE
LFFENSTRTRTTFEIAATRLSADVINLDIARSSASKGESLLDTIANLSAMAADLFVVRHG
EEECCCCCCEEEHHHHHHHHHHHEEEEEEECCCCCCCHHHHHHHHHHHHHHHEEEEEEEC
ESGAPYLIAQHVAPHVHVINAGDGRHAHPTQGLLDMYTIRHYKKDFSNLTVAIVGDVLHS
CCCCCEEEEECCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCEEHHHHHHHHHH
RVARSDIHALTTLGCPEVRVVGPKTLVPADMAQMGVRVCNTLEEGIKGADVIIMLRLQNE
HHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCC
RMSGALLPSSQEFFKSFGLTNEKLQLAKPDAIVMHPGPINRGVEIDSAVVDGTQSVILPQ
CCCCCCCCCHHHHHHHHCCCCCCEEEECCCEEEECCCCCCCCCEECHHHHCCCCCEEHHH
VTFGIAVRMAVMGIVAGNEA
HHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA