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The map label for this gene is ispH

Identifier: 91786853

GI number: 91786853

Start: 973754

End: 974755

Strand: Direct

Name: ispH

Synonym: Bpro_0951

Alternate gene names: 91786853

Gene position: 973754-974755 (Clockwise)

Preceding gene: 91786852

Following gene: 91786854

Centisome position: 18.73

GC content: 64.27

Gene sequence:

>1002_bases
ATGAATACCGCAGACAAACGGCTTGACGAAATCCTGCTCGCGGAGCCGCGCGGCTTTTGCGCCGGTGTCGACCGGGCCAT
TGAAATCGTCGAGCGGGCGCTCACCAAGTTTGGCGCACCGATCTACGTGCGCCACGAAATCGTGCACAACACCTATGTGG
TCAACGAGCTCAAGGCCAAGGGCGCGATCTTTATCGAAGAGCTGTCCGACGTGCCGCCGGGCGCCACGCTGGTGTTCAGC
GCCCATGGCGTGAGCAAGGCCATCCAGGACGAGGCGCGCGCCCGCGGCTTCCAGATTTTTGATGCCACCTGCCCGCTGGT
GACCAAGGTGCACGTCGAAGTGGCCAAGCTGCACAAGGAAGGTTACGAGTTCATCATGATCGGCCACAAGGGCCACCCCG
AGGTGGAGGGCACCATGGGCCAGCTCGACAGCGGCATCCACCTGGTGGAAGACGTGGCCGACGTGGCACGCATTACGCCC
TCGCAAACCGAGCGGCTGGCCGTGGTGACGCAAACCACGCTGAGCGTGGATGACGCGGCCGAGATCAGCGCGGCCGTGAA
GGCGCGCTTTCCGCAGGTGCGCGAGCCCAAGCAGCAGGACATTTGCTACGCCACGCAAAACCGGCAGGACGCCGTCAAGG
TGCTCAGCCCGCAGGTGGACATCCTGATCGTGGTTGGCAGCCCGACAAGTTCGAACAGCAACCGTCTGCGCGAGCTGGCC
GCCAAGCTGGGCACTGAGGCCTACATGGTGGACGATGCCAGCGAGCTGCGGGAGTCCTGGTTCGAGGGCAAGAGCCGCGT
CGGTCTGACGGCCGGCGCCTCGGCCCCGGAGATCCTCGTCAAGCAGGTGATAGACCGCATCCGGGCGCTGGGCGCGGTCT
CCGTGCGCAAGATGGACGGCATCGAGGAAACCATCAAGTTTCCGCTGCCCAAGGGCCTGAAGGTCGATGCCCAGGGCCAC
ATGCTGGACGTCGGCGACAGCCAGCTTCATCAATTGACCTGA

Upstream 100 bases:

>100_bases
TTCAGCAGGTCAAGGAGGGCGGCGATGCCGTGCAGTTCGACTTCAACCACCCGCTGGCCGGCCAGCCGGTGGTGTTTGAA
GTGCAAGTGATTGGCGTTTT

Downstream 100 bases:

>100_bases
CGCCGGCCATGACCAACCGCCATCAAATCGAGAACTGCCTGGGCGCGTTCAATCAGGATTACCGCCACTTTATTCGTCAA
ACGCCGCTGTGGAAACTGCC

Product: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 333; Mature: 333

Protein sequence:

>333_residues
MNTADKRLDEILLAEPRGFCAGVDRAIEIVERALTKFGAPIYVRHEIVHNTYVVNELKAKGAIFIEELSDVPPGATLVFS
AHGVSKAIQDEARARGFQIFDATCPLVTKVHVEVAKLHKEGYEFIMIGHKGHPEVEGTMGQLDSGIHLVEDVADVARITP
SQTERLAVVTQTTLSVDDAAEISAAVKARFPQVREPKQQDICYATQNRQDAVKVLSPQVDILIVVGSPTSSNSNRLRELA
AKLGTEAYMVDDASELRESWFEGKSRVGLTAGASAPEILVKQVIDRIRALGAVSVRKMDGIEETIKFPLPKGLKVDAQGH
MLDVGDSQLHQLT

Sequences:

>Translated_333_residues
MNTADKRLDEILLAEPRGFCAGVDRAIEIVERALTKFGAPIYVRHEIVHNTYVVNELKAKGAIFIEELSDVPPGATLVFS
AHGVSKAIQDEARARGFQIFDATCPLVTKVHVEVAKLHKEGYEFIMIGHKGHPEVEGTMGQLDSGIHLVEDVADVARITP
SQTERLAVVTQTTLSVDDAAEISAAVKARFPQVREPKQQDICYATQNRQDAVKVLSPQVDILIVVGSPTSSNSNRLRELA
AKLGTEAYMVDDASELRESWFEGKSRVGLTAGASAPEILVKQVIDRIRALGAVSVRKMDGIEETIKFPLPKGLKVDAQGH
MLDVGDSQLHQLT
>Mature_333_residues
MNTADKRLDEILLAEPRGFCAGVDRAIEIVERALTKFGAPIYVRHEIVHNTYVVNELKAKGAIFIEELSDVPPGATLVFS
AHGVSKAIQDEARARGFQIFDATCPLVTKVHVEVAKLHKEGYEFIMIGHKGHPEVEGTMGQLDSGIHLVEDVADVARITP
SQTERLAVVTQTTLSVDDAAEISAAVKARFPQVREPKQQDICYATQNRQDAVKVLSPQVDILIVVGSPTSSNSNRLRELA
AKLGTEAYMVDDASELRESWFEGKSRVGLTAGASAPEILVKQVIDRIRALGAVSVRKMDGIEETIKFPLPKGLKVDAQGH
MLDVGDSQLHQLT

Specific function: Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP)

COG id: COG0761

COG function: function code IM; Penicillin tolerance protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispH family

Homologues:

Organism=Escherichia coli, GI1786212, Length=310, Percent_Identity=56.4516129032258, Blast_Score=372, Evalue=1e-104,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPH_POLSJ (Q12EY5)

Other databases:

- EMBL:   CP000316
- RefSeq:   YP_547805.1
- STRING:   Q12EY5
- GeneID:   4011085
- GenomeReviews:   CP000316_GR
- KEGG:   pol:Bpro_0951
- NMPDR:   fig|296591.1.peg.2986
- eggNOG:   COG0761
- HOGENOM:   HBG335228
- OMA:   HMQVARA
- PhylomeDB:   Q12EY5
- ProtClustDB:   PRK01045
- BioCyc:   PSP296591:BPRO_0951-MONOMER
- HAMAP:   MF_00191
- InterPro:   IPR003451
- TIGRFAMs:   TIGR00216

Pfam domain/function: PF02401 LYTB

EC number: =1.17.1.2

Molecular weight: Translated: 36305; Mature: 36305

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTADKRLDEILLAEPRGFCAGVDRAIEIVERALTKFGAPIYVRHEIVHNTYVVNELKAK
CCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHCC
GAIFIEELSDVPPGATLVFSAHGVSKAIQDEARARGFQIFDATCPLVTKVHVEVAKLHKE
CCEEEEHHHCCCCCCEEEEECCCHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHC
GYEFIMIGHKGHPEVEGTMGQLDSGIHLVEDVADVARITPSQTERLAVVTQTTLSVDDAA
CCEEEEECCCCCCCCCCHHHHHCCCHHHHHHHHHHHHCCCCCCCEEEEEEEEEECCCHHH
EISAAVKARFPQVREPKQQDICYATQNRQDAVKVLSPQVDILIVVGSPTSSNSNRLRELA
HHHHHHHHCCCCCCCCCHHCCEEECCCHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHH
AKLGTEAYMVDDASELRESWFEGKSRVGLTAGASAPEILVKQVIDRIRALGAVSVRKMDG
HHHCCCEEEECCHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
IEETIKFPLPKGLKVDAQGHMLDVGDSQLHQLT
HHHHHHCCCCCCCEECCCCCEEECCCHHHHHCC
>Mature Secondary Structure
MNTADKRLDEILLAEPRGFCAGVDRAIEIVERALTKFGAPIYVRHEIVHNTYVVNELKAK
CCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHCC
GAIFIEELSDVPPGATLVFSAHGVSKAIQDEARARGFQIFDATCPLVTKVHVEVAKLHKE
CCEEEEHHHCCCCCCEEEEECCCHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHC
GYEFIMIGHKGHPEVEGTMGQLDSGIHLVEDVADVARITPSQTERLAVVTQTTLSVDDAA
CCEEEEECCCCCCCCCCHHHHHCCCHHHHHHHHHHHHCCCCCCCEEEEEEEEEECCCHHH
EISAAVKARFPQVREPKQQDICYATQNRQDAVKVLSPQVDILIVVGSPTSSNSNRLRELA
HHHHHHHHCCCCCCCCCHHCCEEECCCHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHH
AKLGTEAYMVDDASELRESWFEGKSRVGLTAGASAPEILVKQVIDRIRALGAVSVRKMDG
HHHCCCEEEECCHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
IEETIKFPLPKGLKVDAQGHMLDVGDSQLHQLT
HHHHHHCCCCCCCEECCCCCEEECCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA