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The map label for this gene is psdht [H]

Identifier: 91786854

GI number: 91786854

Start: 974764

End: 975705

Strand: Direct

Name: psdht [H]

Synonym: Bpro_0952

Alternate gene names: 91786854

Gene position: 974764-975705 (Clockwise)

Preceding gene: 91786853

Following gene: 91786855

Centisome position: 18.74

GC content: 67.73

Gene sequence:

>942_bases
ATGACCAACCGCCATCAAATCGAGAACTGCCTGGGCGCGTTCAATCAGGATTACCGCCACTTTATTCGTCAAACGCCGCT
GTGGAAACTGCCCGGCAAGGCGCTGGGCGTGGCCTGCGCGGAAGTCTGGCTCAAGCTGGAGCACCTGCAGACGGGCGGCA
GCTTCAAGGCGCGCGGCATGCTCAACCGGCTGCTGTCCAATCCGATACCGCCCAGCGGGGTGATCGTCGCGTCGGGCGGC
AATGCCGGCATTGCCACCGCGGCAGCCGCCAAAGAGCTGGGTGTGCACTGCGAGGTGTTTGTGCCTGAAGTGTCCAGCCC
GGCCAAGCGCGCCCGTCTCGCCGCGCTGGGCTCCCGGGTCGTGGTCACGGGCGCGGCTTACGCCGACGCGCTGCAGGCCT
GCCTGGCGCGCCAGCAGGAAACCGGCGCGCTGCTCACGCATGCCTATGACCAGCCCGAAGTGCTCACGGGGGCCGGCACG
ATGGCGCTGGAGATCGAGCAGCAGGGCGGCCTGCCCGATGCCGTGCTGGTCAGCGTCGGCGGCGGTGGCCTGATTGGCGG
CGTGGCCAGCTGGTTTGAACAGCGCTCTAGAGTGGTGGCGCTGGAGCCTGAACTGGCGCCAACGCTGTTCAAGGCCCGCG
AAGCCGGCGAGCCGGTGGACGTGGCCATCAGCGGCATTGCAGCCGACTCGCTGGGCGCCAAGCGCATTGGCAGCATGGCG
TGGGACGCGACCCAGGCCCATGTGCGCGATGCGCTGTTGCTCAGCGACGAATCCATCCGGGCGGCGCAGTTGTGGCTCTG
GAACGAACTGAAACTGGCCGTTGAACCCGCCGCCGCGTTGCCGCTGGCCGCGTTGCACAGCGGCCGCTACGTGCCGCGCA
CCGATGAAAAGGTCTGCCTCATCATTTGCGGCGCCAACCTGGACCCAGCGTCGCTTGTCTGA

Upstream 100 bases:

>100_bases
CCATCAAGTTTCCGCTGCCCAAGGGCCTGAAGGTCGATGCCCAGGGCCACATGCTGGACGTCGGCGACAGCCAGCTTCAT
CAATTGACCTGACGCCGGCC

Downstream 100 bases:

>100_bases
AGTTAAATTGGCACAAAAGGGGCTTCTAGAAATTCGATTGTCGGGATGCAGGGCAAGGCGCACGGAGCACAGGACACCGG
AATGCACTTCGGTGCATGAG

Product: threonine dehydratase

Products: 2-oxobutanoate; NH3

Alternate protein names: NA

Number of amino acids: Translated: 313; Mature: 312

Protein sequence:

>313_residues
MTNRHQIENCLGAFNQDYRHFIRQTPLWKLPGKALGVACAEVWLKLEHLQTGGSFKARGMLNRLLSNPIPPSGVIVASGG
NAGIATAAAAKELGVHCEVFVPEVSSPAKRARLAALGSRVVVTGAAYADALQACLARQQETGALLTHAYDQPEVLTGAGT
MALEIEQQGGLPDAVLVSVGGGGLIGGVASWFEQRSRVVALEPELAPTLFKAREAGEPVDVAISGIAADSLGAKRIGSMA
WDATQAHVRDALLLSDESIRAAQLWLWNELKLAVEPAAALPLAALHSGRYVPRTDEKVCLIICGANLDPASLV

Sequences:

>Translated_313_residues
MTNRHQIENCLGAFNQDYRHFIRQTPLWKLPGKALGVACAEVWLKLEHLQTGGSFKARGMLNRLLSNPIPPSGVIVASGG
NAGIATAAAAKELGVHCEVFVPEVSSPAKRARLAALGSRVVVTGAAYADALQACLARQQETGALLTHAYDQPEVLTGAGT
MALEIEQQGGLPDAVLVSVGGGGLIGGVASWFEQRSRVVALEPELAPTLFKAREAGEPVDVAISGIAADSLGAKRIGSMA
WDATQAHVRDALLLSDESIRAAQLWLWNELKLAVEPAAALPLAALHSGRYVPRTDEKVCLIICGANLDPASLV
>Mature_312_residues
TNRHQIENCLGAFNQDYRHFIRQTPLWKLPGKALGVACAEVWLKLEHLQTGGSFKARGMLNRLLSNPIPPSGVIVASGGN
AGIATAAAAKELGVHCEVFVPEVSSPAKRARLAALGSRVVVTGAAYADALQACLARQQETGALLTHAYDQPEVLTGAGTM
ALEIEQQGGLPDAVLVSVGGGGLIGGVASWFEQRSRVVALEPELAPTLFKAREAGEPVDVAISGIAADSLGAKRIGSMAW
DATQAHVRDALLLSDESIRAAQLWLWNELKLAVEPAAALPLAALHSGRYVPRTDEKVCLIICGANLDPASLV

Specific function: Catalyzes The Formation Of Alpha-Ketobutyrate From Threonine In A Two-Step Reaction. The First Step Is A Dehydration Of Threonine, Followed By Rehydration And Liberation Of Ammonia. Deaminates L-Threonine, But Also L-Serine To A Lesser Extent. [C]

COG id: COG1171

COG function: function code E; Threonine dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI11345492, Length=290, Percent_Identity=28.6206896551724, Blast_Score=118, Evalue=7e-27,
Organism=Escherichia coli, GI1790207, Length=296, Percent_Identity=33.1081081081081, Blast_Score=104, Evalue=6e-24,
Organism=Escherichia coli, GI1789505, Length=276, Percent_Identity=30.7971014492754, Blast_Score=90, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI71991565, Length=277, Percent_Identity=30.6859205776173, Blast_Score=97, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI17508781, Length=281, Percent_Identity=27.7580071174377, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17537387, Length=277, Percent_Identity=32.129963898917, Blast_Score=95, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6319788, Length=293, Percent_Identity=27.9863481228669, Blast_Score=103, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6322631, Length=310, Percent_Identity=29.3548387096774, Blast_Score=95, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6320930, Length=284, Percent_Identity=29.2253521126761, Blast_Score=90, Evalue=6e-19,
Organism=Drosophila melanogaster, GI21355833, Length=273, Percent_Identity=32.6007326007326, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24645328, Length=211, Percent_Identity=30.8056872037915, Blast_Score=69, Evalue=4e-12,

Paralogues:

None

Copy number: 1344 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: 4.3.1.19

Molecular weight: Translated: 32885; Mature: 32754

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: PS00165 DEHYDRATASE_SER_THR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNRHQIENCLGAFNQDYRHFIRQTPLWKLPGKALGVACAEVWLKLEHLQTGGSFKARGM
CCCHHHHHHHHHHCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHH
LNRLLSNPIPPSGVIVASGGNAGIATAAAAKELGVHCEVFVPEVSSPAKRARLAALGSRV
HHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHCCEE
VVTGAAYADALQACLARQQETGALLTHAYDQPEVLTGAGTMALEIEQQGGLPDAVLVSVG
EEECHHHHHHHHHHHHHHHCCCEEEEECCCCCCEEECCCEEEEEEECCCCCCCEEEEEEC
GGGLIGGVASWFEQRSRVVALEPELAPTLFKAREAGEPVDVAISGIAADSLGAKRIGSMA
CCCHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHCCCCCEEEEECHHHHCCCHHHHHHHH
WDATQAHVRDALLLSDESIRAAQLWLWNELKLAVEPAAALPLAALHSGRYVPRTDEKVCL
HHHHHHHHHHHHEECCCCCCEEEEEEEECEEEEECCHHHHHHHHHHCCCCCCCCCCCEEE
IICGANLDPASLV
EEECCCCCHHCCC
>Mature Secondary Structure 
TNRHQIENCLGAFNQDYRHFIRQTPLWKLPGKALGVACAEVWLKLEHLQTGGSFKARGM
CCHHHHHHHHHHCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHH
LNRLLSNPIPPSGVIVASGGNAGIATAAAAKELGVHCEVFVPEVSSPAKRARLAALGSRV
HHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHCCEE
VVTGAAYADALQACLARQQETGALLTHAYDQPEVLTGAGTMALEIEQQGGLPDAVLVSVG
EEECHHHHHHHHHHHHHHHCCCEEEEECCCCCCEEECCCEEEEEEECCCCCCCEEEEEEC
GGGLIGGVASWFEQRSRVVALEPELAPTLFKAREAGEPVDVAISGIAADSLGAKRIGSMA
CCCHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHCCCCCEEEEECHHHHCCCHHHHHHHH
WDATQAHVRDALLLSDESIRAAQLWLWNELKLAVEPAAALPLAALHSGRYVPRTDEKVCL
HHHHHHHHHHHHEECCCCCCEEEEEEEECEEEEECCHHHHHHHHHHCCCCCCCCCCCEEE
IICGANLDPASLV
EEECCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: AMP; L-Val; pyridoxal 5'-phosphate [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 40 {L-Ser}} 5 {L-Ser}} 91 {L-Thr}} 70 {L-Thr}} 11 {L-Thr}} [C]

Substrates: L-threonine

Specific reaction: L-threonine = 2-oxobutanoate + NH3

General reaction: Deamination; elimination of NH alphabeta-position of amino acid [C]

Inhibitor: Ile [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12596884; 8743570 [H]