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The map label for this gene is ubiE [H]

Identifier: 91786762

GI number: 91786762

Start: 883755

End: 884486

Strand: Direct

Name: ubiE [H]

Synonym: Bpro_0860

Alternate gene names: 91786762

Gene position: 883755-884486 (Clockwise)

Preceding gene: 91786761

Following gene: 91786763

Centisome position: 16.99

GC content: 57.65

Gene sequence:

>732_bases
ATGACGAAAACCCATTTCGGCTTTGAATCCGTCGAGGAGCAGGACAAGGCGCGACGCGTGCGCGGCGTGTTTGACTCTGT
GGCGCCCAAATACGACGTGATGAATGACTTGATGTCGATGGGTCTGCATCGTGCCTGGAAGGCCTACACCGTGCTGGTGG
CCAACGTTAAGGAGGGCCAGCAGGTGCTCGACATCGCCGGCGGAACGGGCGACCTGGCCCTGGCTTTCGCGCCCAAAGTG
GGCAGCAGCGGGCGGGTGGTGCACACTGACATCAACGAGGCGATGCTCAGGGAAGGGCGTAACCGGCTGCTGGACGCTGG
TGTGAACCTGCCCACGTTGGTCTGTGATGCAGAACACCTGCCGTTTGCCGATGCGAGTTTTGACGTGGTCACCGTCGCTT
TTGGCTTGCGCAACATGACGCATAAAGAAGATGCCTTGCGCGAAATGAACCGTGTGCTCAAGCCCGGTGGCAAACTGCTG
GTGCTGGAGTTTTCCAAGGTGGCCAAACCGCTGGAAAAGATTTATGACTGGTATTCGTTCAAGGTGTTGCCCAGGCTCGG
CAAGCTGGTGGCCAACGACGACTCAAGCTACCAGTACCTCGCCGAGTCGATTCGCATGCATCCGGGCCAGGAAGAACTCA
AAGCCCTGATGCTTAAAGGTGGTTTCGGTCATGTGGACTATCACAACCTGACGGGCGGGGTAGTGGCATTGCATGTTGCA
ATCAAATGCTGA

Upstream 100 bases:

>100_bases
TCCTGGACTGTTGTTTTTCCGGCATAAAACCCCACAATGCCCTGAAGCAGTGCAGGGTTGTCGCCACTAACCAAGGGCAT
TGCCCTCTAGCATTCACTCT

Downstream 100 bases:

>100_bases
AGAAGTGTTGAAGAAGTTAAACCGGCTTAAATAAACAAACAGGCGCAAGGCTTGCTCAAGGAGACGTTATGAAGATTTGG
TCAATCATTCTTTCCGCCGT

Product: 2-octaprenyl-6-methoxy-1,4-benzoquinone methylase/demethylmenaquinone methyltransferase

Products: S-Adenosyl-L-Homocysteine; @OCTA02.txt*2- Polyprenyl-3-Methyl-6-Methoxy-1,4-Benzoquinol!; Menaquinol. [C]

Alternate protein names: NA

Number of amino acids: Translated: 243; Mature: 242

Protein sequence:

>243_residues
MTKTHFGFESVEEQDKARRVRGVFDSVAPKYDVMNDLMSMGLHRAWKAYTVLVANVKEGQQVLDIAGGTGDLALAFAPKV
GSSGRVVHTDINEAMLREGRNRLLDAGVNLPTLVCDAEHLPFADASFDVVTVAFGLRNMTHKEDALREMNRVLKPGGKLL
VLEFSKVAKPLEKIYDWYSFKVLPRLGKLVANDDSSYQYLAESIRMHPGQEELKALMLKGGFGHVDYHNLTGGVVALHVA
IKC

Sequences:

>Translated_243_residues
MTKTHFGFESVEEQDKARRVRGVFDSVAPKYDVMNDLMSMGLHRAWKAYTVLVANVKEGQQVLDIAGGTGDLALAFAPKV
GSSGRVVHTDINEAMLREGRNRLLDAGVNLPTLVCDAEHLPFADASFDVVTVAFGLRNMTHKEDALREMNRVLKPGGKLL
VLEFSKVAKPLEKIYDWYSFKVLPRLGKLVANDDSSYQYLAESIRMHPGQEELKALMLKGGFGHVDYHNLTGGVVALHVA
IKC
>Mature_242_residues
TKTHFGFESVEEQDKARRVRGVFDSVAPKYDVMNDLMSMGLHRAWKAYTVLVANVKEGQQVLDIAGGTGDLALAFAPKVG
SSGRVVHTDINEAMLREGRNRLLDAGVNLPTLVCDAEHLPFADASFDVVTVAFGLRNMTHKEDALREMNRVLKPGGKLLV
LEFSKVAKPLEKIYDWYSFKVLPRLGKLVANDDSSYQYLAESIRMHPGQEELKALMLKGGFGHVDYHNLTGGVVALHVAI
KC

Specific function: Methyltransferase required for the conversion of dimethylmenaquinone (DMKH2) to menaquinone (MKH2) and the conversion of 2-polyprenyl-6-methoxy-1,4-benzoquinol (DDMQH2) to 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinol (DMQH2) [H]

COG id: COG2226

COG function: function code H; Methylase involved in ubiquinone/menaquinone biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. UbiE family [H]

Homologues:

Organism=Homo sapiens, GI116063536, Length=277, Percent_Identity=45.4873646209386, Blast_Score=236, Evalue=2e-62,
Organism=Escherichia coli, GI48994983, Length=242, Percent_Identity=60.3305785123967, Blast_Score=292, Evalue=2e-80,
Organism=Caenorhabditis elegans, GI17557039, Length=244, Percent_Identity=47.1311475409836, Blast_Score=223, Evalue=6e-59,
Organism=Saccharomyces cerevisiae, GI6323526, Length=256, Percent_Identity=40.234375, Blast_Score=173, Evalue=2e-44,
Organism=Drosophila melanogaster, GI24641702, Length=255, Percent_Identity=43.1372549019608, Blast_Score=212, Evalue=2e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004033 [H]

Pfam domain/function: PF01209 Ubie_methyltran [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 26870; Mature: 26739

Theoretical pI: Translated: 7.76; Mature: 7.76

Prosite motif: PS01183 UBIE_1 ; PS01184 UBIE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKTHFGFESVEEQDKARRVRGVFDSVAPKYDVMNDLMSMGLHRAWKAYTVLVANVKEGQ
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHEEEEHHCCC
QVLDIAGGTGDLALAFAPKVGSSGRVVHTDINEAMLREGRNRLLDAGVNLPTLVCDAEHL
EEEEECCCCCCEEEEEECCCCCCCCEEEECHHHHHHHHHHHHHHHCCCCCCEEEECCCCC
PFADASFDVVTVAFGLRNMTHKEDALREMNRVLKPGGKLLVLEFSKVAKPLEKIYDWYSF
CCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHH
KVLPRLGKLVANDDSSYQYLAESIRMHPGQEELKALMLKGGFGHVDYHNLTGGVVALHVA
HHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEE
IKC
EEC
>Mature Secondary Structure 
TKTHFGFESVEEQDKARRVRGVFDSVAPKYDVMNDLMSMGLHRAWKAYTVLVANVKEGQ
CCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHEEEEHHCCC
QVLDIAGGTGDLALAFAPKVGSSGRVVHTDINEAMLREGRNRLLDAGVNLPTLVCDAEHL
EEEEECCCCCCEEEEEECCCCCCCCEEEECHHHHHHHHHHHHHHHCCCCCCEEEECCCCC
PFADASFDVVTVAFGLRNMTHKEDALREMNRVLKPGGKLLVLEFSKVAKPLEKIYDWYSF
CCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHH
KVLPRLGKLVANDDSSYQYLAESIRMHPGQEELKALMLKGGFGHVDYHNLTGGVVALHVA
HHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEE
IKC
EEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-Adenosyl-L-Methionine; 2-Polyprenyl-6- Methoxy-1,4-Benzoquinol; Demethylmenaquinol [C]

Specific reaction: S-Adenosyl-L-Methionine + @OCTA02.txt*2-Polyprenyl-6- Methoxy-1,4-Benzoquinol! = S-Adenosyl-L-Homocysteine + 2- Polyprenyl-3-Methyl-6-Methoxy -1,4-Benzoquinol S-Adenosyl-L-Methionine + Demethylmenaquinol = S-Adenosyl-L-Homocysteine + Menaquinol. [C]

General reaction: Transferring one-carbon groups; Methyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA