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The map label for this gene is pilB [H]

Identifier: 91786743

GI number: 91786743

Start: 863324

End: 865060

Strand: Direct

Name: pilB [H]

Synonym: Bpro_0841

Alternate gene names: 91786743

Gene position: 863324-865060 (Clockwise)

Preceding gene: 91786742

Following gene: 91786744

Centisome position: 16.6

GC content: 58.38

Gene sequence:

>1737_bases
ATGGCCGCTGTCGATACTGCTATCAACGAACCGCCCGCCTTGGCATTGCCCGGATTGGGCCGGGCATTGGTGGTGGCAGG
CAAGCTGGGCCAGAAGGCCGCTGAAGACATTTTTCGCAAGGCCCAAAGCGGGCGCACCAGCTTCATTGCCGAGCTGACCG
GTTCAGGCGCCGTGTCTGCGTTTGACCTGGCGCACACGATGTCCATCGCGTTCGCCGCGCCTTTGCTCGATCTGGAGGCC
ATCGACGTCCAGCGTTTGCCCAAAGGCCTGCTCGACACCAAGATTTGCACGACTTACCGCATTGTCGTGCTGAGCAAGCG
CAATAACCGCCTGATCGTCGCAACGGCAGATCCCTCTGACCAGGAAGCGGCGGAAAAGATCAAGTTTTCCACCCAGATGG
GTGTGGACTGGGTGATTGCCGAGTATGACAAGCTGTCCAAACTGGTGGAGGCGTCGAGTACCACGGCTGCCGAGGCCATG
GAGAGCATCATCGGCGATGATTTCGAGTTCGATGAATCTTCGGTGGATTCAGGCGCTGCTGAAGACAGCGATGCCAGCGT
CTCTGAAGTCGAAGATGCACCGGTCGTCAAATTTCTCCAGAAGATGCTGCTCGATGCCTTCAGCATGCGGGCCTCTGATT
TGCACTTTGAGCCGTTCGAGCACACCTACCGTGTCCGTTTCCGGATCGACGGCGAATTGCGCGAAATTGCTTCGCCGCCC
GTGGCCATCAAGGAAAAACTCGCGGCACGTATCAAGGTGATCTCCAAGCTGGATATCTCGGAAAAGCGGGTGCCGCAGGA
TGGCAAGATGAAGCTCAAGATCGGCCCTGACCGCGTGATCGATTTTCGGGTCAGCACCTTGCCCACCATGTACGGTGAAA
AGGTCGTGATCCGTATTCTGGACCCGAGCAGTGCCAAACTGGGTATTGACGCGCTGGGTTACGAGCCGGTTGAGAAGGAG
CGCTTGCTGGCGGCCATCCAGCGGCCCTATGGCATGGTGCTGGTGACCGGCCCGACCGGCTCCGGCAAGACAGTGTCGCT
GTACACCTGCCTGAATATCCTGAACAAGCCGGGCGTCAACATTGCCACGGCTGAAGACCCTGCCGAGATCACCTTGCCAG
GCGTCAACCAGGTCAGCATGAACGACAAGGCGGGCATGACTTTCTCTGTCGCGCTCAAGGCCTTTTTGCGTCAGGATCCG
GACATCATCATGGTGGGTGAGATCCGCGACATCGAAACCGCCGACATCGCGATCAAGGCGGCCCAGACCGGCCACATGGT
CATGTCAACGCTGCATACCAACGACGCGCCAACCACCTTGACCCGCATGCGCAATATGGGCATTGCGCCCTTCAATATTG
CATCGAGCGTGATCCTGATCACGGCGCAGCGGCTGGCGCGGCGCCTGTGTGTTAACTGCAAGGCGCCCGCAGATATTCCG
CGCGAAACCTTGCTGGAGGCAGGATTCAAGGAAGAAGAAGTCGATGGTTCCTGGACGCCATATCGCCCGGTGGGCTGTTC
CATGTGTACAAATGGTTACAAGGGTCGCGTTGGCATCTACCAGGTCATGCCTATTTCCGAAGAGATCCAGCGGATAGTGT
TGCGCGACGGCAGCGCCCTTGAGATTGCGGCGCAGGCAGAAGCTGAGGGTGTCAGGAGTTTGCGCCAGTCCGGCCTGTCT
AAAGTTAAACTCGGCACGACCTCCCTGGAAGAGGTTTTGGGTTGCACCAATGTCTAA

Upstream 100 bases:

>100_bases
CCGCACAGTTGCAACGCTTTGGGGTGGGGCGGGGGTGTTTTCGATTTACACCTTGTTATCTATCGACGATGATACCCATT
TGGGCTAATTTCAAAGATTC

Downstream 100 bases:

>100_bases
TGGGCGTGCAAAGTTGAACACACTGGTGAGTGACTGTGGCGACGGGGTGGCCTGAGGCCATCCATACAACGAGAAAACAT
CATGGCAACTGCAGTATCCA

Product: type II secretion system protein E

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 578; Mature: 577

Protein sequence:

>578_residues
MAAVDTAINEPPALALPGLGRALVVAGKLGQKAAEDIFRKAQSGRTSFIAELTGSGAVSAFDLAHTMSIAFAAPLLDLEA
IDVQRLPKGLLDTKICTTYRIVVLSKRNNRLIVATADPSDQEAAEKIKFSTQMGVDWVIAEYDKLSKLVEASSTTAAEAM
ESIIGDDFEFDESSVDSGAAEDSDASVSEVEDAPVVKFLQKMLLDAFSMRASDLHFEPFEHTYRVRFRIDGELREIASPP
VAIKEKLAARIKVISKLDISEKRVPQDGKMKLKIGPDRVIDFRVSTLPTMYGEKVVIRILDPSSAKLGIDALGYEPVEKE
RLLAAIQRPYGMVLVTGPTGSGKTVSLYTCLNILNKPGVNIATAEDPAEITLPGVNQVSMNDKAGMTFSVALKAFLRQDP
DIIMVGEIRDIETADIAIKAAQTGHMVMSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCVNCKAPADIP
RETLLEAGFKEEEVDGSWTPYRPVGCSMCTNGYKGRVGIYQVMPISEEIQRIVLRDGSALEIAAQAEAEGVRSLRQSGLS
KVKLGTTSLEEVLGCTNV

Sequences:

>Translated_578_residues
MAAVDTAINEPPALALPGLGRALVVAGKLGQKAAEDIFRKAQSGRTSFIAELTGSGAVSAFDLAHTMSIAFAAPLLDLEA
IDVQRLPKGLLDTKICTTYRIVVLSKRNNRLIVATADPSDQEAAEKIKFSTQMGVDWVIAEYDKLSKLVEASSTTAAEAM
ESIIGDDFEFDESSVDSGAAEDSDASVSEVEDAPVVKFLQKMLLDAFSMRASDLHFEPFEHTYRVRFRIDGELREIASPP
VAIKEKLAARIKVISKLDISEKRVPQDGKMKLKIGPDRVIDFRVSTLPTMYGEKVVIRILDPSSAKLGIDALGYEPVEKE
RLLAAIQRPYGMVLVTGPTGSGKTVSLYTCLNILNKPGVNIATAEDPAEITLPGVNQVSMNDKAGMTFSVALKAFLRQDP
DIIMVGEIRDIETADIAIKAAQTGHMVMSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCVNCKAPADIP
RETLLEAGFKEEEVDGSWTPYRPVGCSMCTNGYKGRVGIYQVMPISEEIQRIVLRDGSALEIAAQAEAEGVRSLRQSGLS
KVKLGTTSLEEVLGCTNV
>Mature_577_residues
AAVDTAINEPPALALPGLGRALVVAGKLGQKAAEDIFRKAQSGRTSFIAELTGSGAVSAFDLAHTMSIAFAAPLLDLEAI
DVQRLPKGLLDTKICTTYRIVVLSKRNNRLIVATADPSDQEAAEKIKFSTQMGVDWVIAEYDKLSKLVEASSTTAAEAME
SIIGDDFEFDESSVDSGAAEDSDASVSEVEDAPVVKFLQKMLLDAFSMRASDLHFEPFEHTYRVRFRIDGELREIASPPV
AIKEKLAARIKVISKLDISEKRVPQDGKMKLKIGPDRVIDFRVSTLPTMYGEKVVIRILDPSSAKLGIDALGYEPVEKER
LLAAIQRPYGMVLVTGPTGSGKTVSLYTCLNILNKPGVNIATAEDPAEITLPGVNQVSMNDKAGMTFSVALKAFLRQDPD
IIMVGEIRDIETADIAIKAAQTGHMVMSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCVNCKAPADIPR
ETLLEAGFKEEEVDGSWTPYRPVGCSMCTNGYKGRVGIYQVMPISEEIQRIVLRDGSALEIAAQAEAEGVRSLRQSGLSK
VKLGTTSLEEVLGCTNV

Specific function: Involved in the translocation of the type IV pilin (pilA) [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=443, Percent_Identity=42.6636568848758, Blast_Score=338, Evalue=6e-94,
Organism=Escherichia coli, GI1786296, Length=491, Percent_Identity=36.6598778004073, Blast_Score=325, Evalue=5e-90,
Organism=Escherichia coli, GI87082188, Length=236, Percent_Identity=30.9322033898305, Blast_Score=99, Evalue=6e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013374
- InterPro:   IPR007831
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E; PF05157 GSPII_E_N [H]

EC number: NA

Molecular weight: Translated: 62519; Mature: 62388

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAVDTAINEPPALALPGLGRALVVAGKLGQKAAEDIFRKAQSGRTSFIAELTGSGAVSA
CCCCCCCCCCCCCEECCCCCCEEEEECHHCHHHHHHHHHHHHCCCCEEEEEECCCCCCHH
FDLAHTMSIAFAAPLLDLEAIDVQRLPKGLLDTKICTTYRIVVLSKRNNRLIVATADPSD
HHHHHHHHHHHHHHHHCCHHHCHHHCCHHHHHHHHHCEEEEEEEECCCCEEEEEECCCCC
QEAAEKIKFSTQMGVDWVIAEYDKLSKLVEASSTTAAEAMESIIGDDFEFDESSVDSGAA
HHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCC
EDSDASVSEVEDAPVVKFLQKMLLDAFSMRASDLHFEPFEHTYRVRFRIDGELREIASPP
CCCCCCHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEECCHHHHHCCCC
VAIKEKLAARIKVISKLDISEKRVPQDGKMKLKIGPDRVIDFRVSTLPTMYGEKVVIRIL
HHHHHHHHHHHHHHHHCCCCHHCCCCCCCEEEEECCCCEEEEEHHHCCCCCCCEEEEEEE
DPSSAKLGIDALGYEPVEKERLLAAIQRPYGMVLVTGPTGSGKTVSLYTCLNILNKPGVN
CCCCCCCCCHHCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCEEEHHHHHHHHCCCCCE
IATAEDPAEITLPGVNQVSMNDKAGMTFSVALKAFLRQDPDIIMVGEIRDIETADIAIKA
EEECCCCCEEEECCCCEECCCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCCCEEEEE
AQTGHMVMSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCVNCKAPADIP
CCCCHHHHHHHCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
RETLLEAGFKEEEVDGSWTPYRPVGCSMCTNGYKGRVGIYQVMPISEEIQRIVLRDGSAL
HHHHHHCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCEEEEEECCCHHHHHHHHHCCCCEE
EIAAQAEAEGVRSLRQSGLSKVKLGTTSLEEVLGCTNV
EEEEHHHHHHHHHHHHCCCCEEEECCHHHHHHHCCCCC
>Mature Secondary Structure 
AAVDTAINEPPALALPGLGRALVVAGKLGQKAAEDIFRKAQSGRTSFIAELTGSGAVSA
CCCCCCCCCCCCEECCCCCCEEEEECHHCHHHHHHHHHHHHCCCCEEEEEECCCCCCHH
FDLAHTMSIAFAAPLLDLEAIDVQRLPKGLLDTKICTTYRIVVLSKRNNRLIVATADPSD
HHHHHHHHHHHHHHHHCCHHHCHHHCCHHHHHHHHHCEEEEEEEECCCCEEEEEECCCCC
QEAAEKIKFSTQMGVDWVIAEYDKLSKLVEASSTTAAEAMESIIGDDFEFDESSVDSGAA
HHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCC
EDSDASVSEVEDAPVVKFLQKMLLDAFSMRASDLHFEPFEHTYRVRFRIDGELREIASPP
CCCCCCHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEECCHHHHHCCCC
VAIKEKLAARIKVISKLDISEKRVPQDGKMKLKIGPDRVIDFRVSTLPTMYGEKVVIRIL
HHHHHHHHHHHHHHHHCCCCHHCCCCCCCEEEEECCCCEEEEEHHHCCCCCCCEEEEEEE
DPSSAKLGIDALGYEPVEKERLLAAIQRPYGMVLVTGPTGSGKTVSLYTCLNILNKPGVN
CCCCCCCCCHHCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCEEEHHHHHHHHCCCCCE
IATAEDPAEITLPGVNQVSMNDKAGMTFSVALKAFLRQDPDIIMVGEIRDIETADIAIKA
EEECCCCCEEEECCCCEECCCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCCCEEEEE
AQTGHMVMSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCVNCKAPADIP
CCCCHHHHHHHCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
RETLLEAGFKEEEVDGSWTPYRPVGCSMCTNGYKGRVGIYQVMPISEEIQRIVLRDGSAL
HHHHHHCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCEEEEEECCCHHHHHHHHHCCCCEE
EIAAQAEAEGVRSLRQSGLSKVKLGTTSLEEVLGCTNV
EEEEHHHHHHHHHHHHCCCCEEEECCHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1971619; 10984043 [H]