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The map label for this gene is ispB [H]

Identifier: 91786742

GI number: 91786742

Start: 862114

End: 863097

Strand: Direct

Name: ispB [H]

Synonym: Bpro_0840

Alternate gene names: 91786742

Gene position: 862114-863097 (Clockwise)

Preceding gene: 91786737

Following gene: 91786743

Centisome position: 16.58

GC content: 62.91

Gene sequence:

>984_bases
TTGTCTGCCAATTCCTCAAGCACCGCCGCCGCCCTTGCCCTGATTGCCGACGACATGCGTGGAATGGACGCGGTGATCGA
GCGCCGGCTGAAATCGGACGTGCCACTGGTCAGCCAAGTGTCGCAATATATCGTGGCGGCCGGCGGCAAGCGCCTGCGGC
CCGCCCTGCTGCTTCTGATGTGTGGTGCTCTGGGTTATCGCGGCGATCAGCGCTACAACCTGGCGGCGGTGGTGGAGTTC
ATCCACACCGCCACCTTGCTGCATGATGACGTGGTGGATGAGTCCACGCTGCGCCGCGGCCGAGCCACGGCCAACGAGGC
CTTCGGCAACCCTGCGAGCGTGCTGGTCGGGGATTTTCTATATTCCCGCGCCTTCCAGATGATGGTGGAGGCCGGGGACA
TGCGCGTCATGCAGACACTCGCGGAGGCCACCAATGTGATTGCCGAGGGCGAAGTGCTGCAGCTGATGAACATGCATGAC
GCCACGCTGTCAGAGGACGGTTACCTGCGGGTGATCCGTTCCAAGACGGCCAAGCTGTTCGAGGCCAGCGCCCGTCTGGC
GGCATTGCTGGCGCAGTCCCCCCCGCCCGTGGAGCAAAGCTGCGCCGATTATGGACAGGCGCTGGGCACCGCTTTCCAGG
TGATTGACGATGTGCTGGACTATGACGGCGATGTATCGGAAATGGGGAAAAACCTGGGGGACGACCTGCGCGAAGGCAAG
ACGACGCTGCCGCTCATCATTGCGATGCAGCGATGCACCGAACTTGAGCGTGCCGTGATCCGGCGGGCCATTGAAACAGG
CGGAACGGACCAGATGCCGCAGATCATTGCCATCGTGCAACAAACGGGAGCCCTGCAGGCAACACGGGACGCGGCCGCCG
CGGAGGCCCAGCGCGCGCTGGATGCCTTGCAAGTATTGCCCCAAAACCCTTACAGTAATGCCTTGGGGCATCTGGCATCT
CAACTGCTGCAGAGACGCTCCTGA

Upstream 100 bases:

>100_bases
CAGGCCGGCGGTTTTGCGGCTATTTCGCCGGGGGGGCAACCGGCCGCCGGGGATGGGGTGCTTTCTATAATCTGCCCAAA
CTTTACGTCTGGACCGCCTT

Downstream 100 bases:

>100_bases
CGGTTTGTGAAGGCGGGGTGTAGCTTAGCCTGGTAGAGCGCTACGTTCGGGACGTAGAGGCCGGAGGTTCGAATCCTCTC
ACCCCGACCAGATTCTTTTT

Product: trans-hexaprenyltranstransferase

Products: NA

Alternate protein names: All-trans-octaprenyl-diphosphate synthase; Octaprenyl pyrophosphate synthase; OPP synthase [H]

Number of amino acids: Translated: 327; Mature: 326

Protein sequence:

>327_residues
MSANSSSTAAALALIADDMRGMDAVIERRLKSDVPLVSQVSQYIVAAGGKRLRPALLLLMCGALGYRGDQRYNLAAVVEF
IHTATLLHDDVVDESTLRRGRATANEAFGNPASVLVGDFLYSRAFQMMVEAGDMRVMQTLAEATNVIAEGEVLQLMNMHD
ATLSEDGYLRVIRSKTAKLFEASARLAALLAQSPPPVEQSCADYGQALGTAFQVIDDVLDYDGDVSEMGKNLGDDLREGK
TTLPLIIAMQRCTELERAVIRRAIETGGTDQMPQIIAIVQQTGALQATRDAAAAEAQRALDALQVLPQNPYSNALGHLAS
QLLQRRS

Sequences:

>Translated_327_residues
MSANSSSTAAALALIADDMRGMDAVIERRLKSDVPLVSQVSQYIVAAGGKRLRPALLLLMCGALGYRGDQRYNLAAVVEF
IHTATLLHDDVVDESTLRRGRATANEAFGNPASVLVGDFLYSRAFQMMVEAGDMRVMQTLAEATNVIAEGEVLQLMNMHD
ATLSEDGYLRVIRSKTAKLFEASARLAALLAQSPPPVEQSCADYGQALGTAFQVIDDVLDYDGDVSEMGKNLGDDLREGK
TTLPLIIAMQRCTELERAVIRRAIETGGTDQMPQIIAIVQQTGALQATRDAAAAEAQRALDALQVLPQNPYSNALGHLAS
QLLQRRS
>Mature_326_residues
SANSSSTAAALALIADDMRGMDAVIERRLKSDVPLVSQVSQYIVAAGGKRLRPALLLLMCGALGYRGDQRYNLAAVVEFI
HTATLLHDDVVDESTLRRGRATANEAFGNPASVLVGDFLYSRAFQMMVEAGDMRVMQTLAEATNVIAEGEVLQLMNMHDA
TLSEDGYLRVIRSKTAKLFEASARLAALLAQSPPPVEQSCADYGQALGTAFQVIDDVLDYDGDVSEMGKNLGDDLREGKT
TLPLIIAMQRCTELERAVIRRAIETGGTDQMPQIIAIVQQTGALQATRDAAAAEAQRALDALQVLPQNPYSNALGHLASQ
LLQRRS

Specific function: Supplies octaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinones ubiquinone and menaquinone [H]

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=315, Percent_Identity=30.7936507936508, Blast_Score=146, Evalue=3e-35,
Organism=Homo sapiens, GI4758430, Length=268, Percent_Identity=23.134328358209, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI83700220, Length=268, Percent_Identity=23.134328358209, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI1789578, Length=313, Percent_Identity=53.9936102236422, Blast_Score=337, Evalue=5e-94,
Organism=Escherichia coli, GI1786623, Length=246, Percent_Identity=32.520325203252, Blast_Score=96, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17505681, Length=300, Percent_Identity=31.6666666666667, Blast_Score=149, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6319475, Length=298, Percent_Identity=31.8791946308725, Blast_Score=129, Evalue=6e-31,
Organism=Saccharomyces cerevisiae, GI6325188, Length=276, Percent_Identity=25, Blast_Score=89, Evalue=8e-19,
Organism=Drosophila melanogaster, GI24651612, Length=322, Percent_Identity=28.2608695652174, Blast_Score=139, Evalue=3e-33,
Organism=Drosophila melanogaster, GI281365769, Length=275, Percent_Identity=24.3636363636364, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24660002, Length=275, Percent_Identity=24.3636363636364, Blast_Score=73, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.90 [H]

Molecular weight: Translated: 35250; Mature: 35119

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS00723 POLYPRENYL_SYNTHET_1 ; PS00444 POLYPRENYL_SYNTHET_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSANSSSTAAALALIADDMRGMDAVIERRLKSDVPLVSQVSQYIVAAGGKRLRPALLLLM
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHH
CGALGYRGDQRYNLAAVVEFIHTATLLHDDVVDESTLRRGRATANEAFGNPASVLVGDFL
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCHHHHHCCCHHHHHHHHHH
YSRAFQMMVEAGDMRVMQTLAEATNVIAEGEVLQLMNMHDATLSEDGYLRVIRSKTAKLF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
EASARLAALLAQSPPPVEQSCADYGQALGTAFQVIDDVLDYDGDVSEMGKNLGDDLREGK
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCC
TTLPLIIAMQRCTELERAVIRRAIETGGTDQMPQIIAIVQQTGALQATRDAAAAEAQRAL
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
DALQVLPQNPYSNALGHLASQLLQRRS
HHHHHCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SANSSSTAAALALIADDMRGMDAVIERRLKSDVPLVSQVSQYIVAAGGKRLRPALLLLM
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHH
CGALGYRGDQRYNLAAVVEFIHTATLLHDDVVDESTLRRGRATANEAFGNPASVLVGDFL
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCHHHHHCCCHHHHHHHHHH
YSRAFQMMVEAGDMRVMQTLAEATNVIAEGEVLQLMNMHDATLSEDGYLRVIRSKTAKLF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
EASARLAALLAQSPPPVEQSCADYGQALGTAFQVIDDVLDYDGDVSEMGKNLGDDLREGK
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCC
TTLPLIIAMQRCTELERAVIRRAIETGGTDQMPQIIAIVQQTGALQATRDAAAAEAQRAL
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
DALQVLPQNPYSNALGHLASQLLQRRS
HHHHHCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8312607; 9278503; 2670911; 8037730 [H]