Click here to switch to the map view.

The map label for this gene is htrB [H]

Identifier: 91786670

GI number: 91786670

Start: 788919

End: 789800

Strand: Direct

Name: htrB [H]

Synonym: Bpro_0768

Alternate gene names: 91786670

Gene position: 788919-789800 (Clockwise)

Preceding gene: 91786667

Following gene: 91786671

Centisome position: 15.17

GC content: 67.69

Gene sequence:

>882_bases
ATGCGCTGGTTATTCCGTTTCTTCTCCCTCTGGCCGCTGGCGGCCCTGCACGCCCTGGGTGGGCTGCTGGGCTGGCTGGT
CTGGGCCCTGAGTCCGCGCTACCGGGCGCAGTTCAGGGCCCATGTGGCACAGGCCGGCCTGCCTTTTGAGGTTGCCCGGC
CGGCGATCGCCGAGGCCGGCCGTTTTGTGGCCGAGTTGCCCAAGCTGTGGTTGCGCCCCCCGGGCGAGTCCTGCCTGGGC
AATGTCCGTGTGGAGGGGCAGTCCCACGCAACAGAGGGTTTTGCGCGGGGCAAGGGCGTGATCTTCTTCGGCCCCCACTG
CGGAAGCTTCGAGCTTGGCCCGCAGGCCCTGGCCGAACTGTACGGGCCGATCACCGCCATTTACCGCCCGGCGCGCAAGC
CCTGGCTGGCCCGTCTGGAGCGGCTCGCGCGCGACCGGCCGAATCTCACCGTGATGCCAGCCTCCCTGAGCGGCATTCGC
CAGATGCACAGGACCCTGAAGTCCAACCATGCGGTGGCCATGCTGACGGACCAGGTGCCGCCCGAGGGGCTGGGCGTCTG
GGCGCCTTTTTTTGGCCGGCCGGCCTACACCATGACGCTGGCTGCCCGGCTGGCGCTGCAAAGCGGTGCCGCACTGCTGC
CCGTGAGTTGTGAGCGTTTGCCGCGCGGGCGGGGTTATTGGCTGAAAATCTGGCCACCGGTGGCGGGACTGGATACGCGC
GGCACGACCGATGTGTCCCGCGAAGCCGATATGCTGCGTATCGTGACGCTGATCAACCAGGCGATCGAGGCCATCGTGCT
GAGCCAGCCTGGGCAGTATTTGTGGGGTTATGCGCGCTACAAGACCCCCCGCCACGAAACACCCCGCAAGGACGCCCTTT
GA

Upstream 100 bases:

>100_bases
TTTGGTTTAATGTCGCCCTGCAAGTTGTTCAGTAACTCGGCGACCCTTGCATTATAAGACCTTGCGTTTTTGCCTGAAGC
CGCTTCCCAACACCCCACAC

Downstream 100 bases:

>100_bases
AAACACCCCGTCAGTTAGCCAGCGAATTCACCACCCGCGCGGGCATCGTCTTCATGCAGGTGCTGGCGCTGTTGCCCTTG
TCGTGGATTCGCGCGCTGGG

Product: lipid A biosynthesis acyltransferase

Products: NA

Alternate protein names: Heat shock protein B [H]

Number of amino acids: Translated: 293; Mature: 293

Protein sequence:

>293_residues
MRWLFRFFSLWPLAALHALGGLLGWLVWALSPRYRAQFRAHVAQAGLPFEVARPAIAEAGRFVAELPKLWLRPPGESCLG
NVRVEGQSHATEGFARGKGVIFFGPHCGSFELGPQALAELYGPITAIYRPARKPWLARLERLARDRPNLTVMPASLSGIR
QMHRTLKSNHAVAMLTDQVPPEGLGVWAPFFGRPAYTMTLAARLALQSGAALLPVSCERLPRGRGYWLKIWPPVAGLDTR
GTTDVSREADMLRIVTLINQAIEAIVLSQPGQYLWGYARYKTPRHETPRKDAL

Sequences:

>Translated_293_residues
MRWLFRFFSLWPLAALHALGGLLGWLVWALSPRYRAQFRAHVAQAGLPFEVARPAIAEAGRFVAELPKLWLRPPGESCLG
NVRVEGQSHATEGFARGKGVIFFGPHCGSFELGPQALAELYGPITAIYRPARKPWLARLERLARDRPNLTVMPASLSGIR
QMHRTLKSNHAVAMLTDQVPPEGLGVWAPFFGRPAYTMTLAARLALQSGAALLPVSCERLPRGRGYWLKIWPPVAGLDTR
GTTDVSREADMLRIVTLINQAIEAIVLSQPGQYLWGYARYKTPRHETPRKDAL
>Mature_293_residues
MRWLFRFFSLWPLAALHALGGLLGWLVWALSPRYRAQFRAHVAQAGLPFEVARPAIAEAGRFVAELPKLWLRPPGESCLG
NVRVEGQSHATEGFARGKGVIFFGPHCGSFELGPQALAELYGPITAIYRPARKPWLARLERLARDRPNLTVMPASLSGIR
QMHRTLKSNHAVAMLTDQVPPEGLGVWAPFFGRPAYTMTLAARLALQSGAALLPVSCERLPRGRGYWLKIWPPVAGLDTR
GTTDVSREADMLRIVTLINQAIEAIVLSQPGQYLWGYARYKTPRHETPRKDAL

Specific function: Acylates the intermediate (KDO)2-lipid IVA to form (KDO)2-(lauroyl)-lipid IVA. Has 10 fold selectivity for lauroyl- ACP over myristoyl-ACP [H]

COG id: COG1560

COG function: function code M; Lauroyl/myristoyl acyltransferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the htrB/msbB family [H]

Homologues:

Organism=Escherichia coli, GI1787292, Length=202, Percent_Identity=28.7128712871287, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI87082094, Length=200, Percent_Identity=29.5, Blast_Score=68, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004960
- InterPro:   IPR011920 [H]

Pfam domain/function: PF03279 Lip_A_acyltrans [H]

EC number: 2.3.1.-

Molecular weight: Translated: 32589; Mature: 32589

Theoretical pI: Translated: 10.96; Mature: 10.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRWLFRFFSLWPLAALHALGGLLGWLVWALSPRYRAQFRAHVAQAGLPFEVARPAIAEAG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
RFVAELPKLWLRPPGESCLGNVRVEGQSHATEGFARGKGVIFFGPHCGSFELGPQALAEL
HHHHHHHHHHCCCCCHHHHCCEEECCCCHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHH
YGPITAIYRPARKPWLARLERLARDRPNLTVMPASLSGIRQMHRTLKSNHAVAMLTDQVP
HHHHHHHHCCCCCHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHCCCEEEEEECCCC
PEGLGVWAPFFGRPAYTMTLAARLALQSGAALLPVSCERLPRGRGYWLKIWPPVAGLDTR
CCCCEECCHHHCCCHHHHHHHHHHHHHCCCEEECCCHHHCCCCCCEEEEECCCCCCCCCC
GTTDVSREADMLRIVTLINQAIEAIVLSQPGQYLWGYARYKTPRHETPRKDAL
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MRWLFRFFSLWPLAALHALGGLLGWLVWALSPRYRAQFRAHVAQAGLPFEVARPAIAEAG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
RFVAELPKLWLRPPGESCLGNVRVEGQSHATEGFARGKGVIFFGPHCGSFELGPQALAEL
HHHHHHHHHHCCCCCHHHHCCEEECCCCHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHH
YGPITAIYRPARKPWLARLERLARDRPNLTVMPASLSGIRQMHRTLKSNHAVAMLTDQVP
HHHHHHHHCCCCCHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHCCCEEEEEECCCC
PEGLGVWAPFFGRPAYTMTLAARLALQSGAALLPVSCERLPRGRGYWLKIWPPVAGLDTR
CCCCEECCHHHCCCHHHHHHHHHHHHHCCCEEECCCHHHCCCCCCEEEEECCCCCCCCCC
GTTDVSREADMLRIVTLINQAIEAIVLSQPGQYLWGYARYKTPRHETPRKDAL
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Acyltransferases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]