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The map label for this gene is recF [H]

Identifier: 89256653

GI number: 89256653

Start: 1286228

End: 1287277

Strand: Direct

Name: recF [H]

Synonym: FTL_1352

Alternate gene names: 89256653

Gene position: 1286228-1287277 (Clockwise)

Preceding gene: 89256652

Following gene: 89256654

Centisome position: 67.84

GC content: 26.29

Gene sequence:

>1050_bases
ATGTATATATCTAACTTACGCTTACAAAATTTTAGAAATATACCTGCTAAAAGCTTTGATTTTAAAAATAGTATAAATTT
TATAGTTGGTAAAAATGGTTCAGGTAAAACCTCAATTCTTGAATCAATATATTTTTTATCACATAGTCGTTCTTTTCGTA
GCTCACAGTTAAATAGAATTATTAACCATAATGCTGATGAGTTTATAATCTATACTAAAGCTTATAATCCAGATGAAATT
ACTATCTCGCTATCACGCAAAAAAAATAGTAATAATATCTCAAAGCTAAATTTAGAAATACAAAAAAACCATACAGAAAT
AACTAGAAACTTACCCATTCAATTGATCAATCCCGAGAGTTTTAATATCATAAACTCTGGAGCACAACAAAGATGTAAAG
TGATTGACTGGGGTGCTTTTTATCTTGATAAAACATTTTTAAAAATATGGCAACAAACTAAATTTCTCGTTAAACAAAGA
AATTCTGCTTTAAAACAAAACTATCCATACAGCTATATACTTAGTATAGATAAAAAACTCTGTGAGTTTGCTGAAATACT
TGACTATAAAAGGCAAGCCTATTTTACAAAATTAAAACCTAAAATCTATGAAATATTATCGCATTTTAATCCAAACTTAC
AGCTTGATATAGATTACTTTCGAGGTTGGAACTTACATAAAAGTCTAGCACAAGTGCTAGAAGAATCTTTTAATTATGAC
AATAAATATAAAGTCACTAATCATGGTCCACATAAGGCTGATATAGTTCTAAGTGTAAGTCATAAACCTATCCAAGATAT
CTTTTCACGTGGACAACAAAAATTACTTATTTGTGCACTAAAATTAGCTCAGGGAGAAATTCATAATTCAGAAAATGACA
ATAAATGTATTTATTTAATAGATGATATAACTTCTGAATTAGATAGTATTCATACTCTTACTTTGTTTAATTATCTCAAA
CAACTTAAATCACAAGTTTTTATAACAACTACAGAAAAAAATAAAATTAATGAATTTATTGACACAAATAGCTATATATT
AGAAATATAA

Upstream 100 bases:

>100_bases
CATCCCTAAGATTTTTGAGTTATTAAGCAATATTTATCAACTAAATCCTGATATACTAAAACAACAAATTTATAATACTA
GCTTAGAATTTATCTAGAAA

Downstream 100 bases:

>100_bases
AACTTATAACAATTAAAAATCATGAAAAAACTCATAATTATGACTTTAACAATCCTACCTACTTTAGTAGCAGCAGATAA
AGTTTGCGATGGTAATACTT

Product: DNA replication and repair protein recF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 349; Mature: 349

Protein sequence:

>349_residues
MYISNLRLQNFRNIPAKSFDFKNSINFIVGKNGSGKTSILESIYFLSHSRSFRSSQLNRIINHNADEFIIYTKAYNPDEI
TISLSRKKNSNNISKLNLEIQKNHTEITRNLPIQLINPESFNIINSGAQQRCKVIDWGAFYLDKTFLKIWQQTKFLVKQR
NSALKQNYPYSYILSIDKKLCEFAEILDYKRQAYFTKLKPKIYEILSHFNPNLQLDIDYFRGWNLHKSLAQVLEESFNYD
NKYKVTNHGPHKADIVLSVSHKPIQDIFSRGQQKLLICALKLAQGEIHNSENDNKCIYLIDDITSELDSIHTLTLFNYLK
QLKSQVFITTTEKNKINEFIDTNSYILEI

Sequences:

>Translated_349_residues
MYISNLRLQNFRNIPAKSFDFKNSINFIVGKNGSGKTSILESIYFLSHSRSFRSSQLNRIINHNADEFIIYTKAYNPDEI
TISLSRKKNSNNISKLNLEIQKNHTEITRNLPIQLINPESFNIINSGAQQRCKVIDWGAFYLDKTFLKIWQQTKFLVKQR
NSALKQNYPYSYILSIDKKLCEFAEILDYKRQAYFTKLKPKIYEILSHFNPNLQLDIDYFRGWNLHKSLAQVLEESFNYD
NKYKVTNHGPHKADIVLSVSHKPIQDIFSRGQQKLLICALKLAQGEIHNSENDNKCIYLIDDITSELDSIHTLTLFNYLK
QLKSQVFITTTEKNKINEFIDTNSYILEI
>Mature_349_residues
MYISNLRLQNFRNIPAKSFDFKNSINFIVGKNGSGKTSILESIYFLSHSRSFRSSQLNRIINHNADEFIIYTKAYNPDEI
TISLSRKKNSNNISKLNLEIQKNHTEITRNLPIQLINPESFNIINSGAQQRCKVIDWGAFYLDKTFLKIWQQTKFLVKQR
NSALKQNYPYSYILSIDKKLCEFAEILDYKRQAYFTKLKPKIYEILSHFNPNLQLDIDYFRGWNLHKSLAQVLEESFNYD
NKYKVTNHGPHKADIVLSVSHKPIQDIFSRGQQKLLICALKLAQGEIHNSENDNKCIYLIDDITSELDSIHTLTLFNYLK
QLKSQVFITTTEKNKINEFIDTNSYILEI

Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP [H]

COG id: COG1195

COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recF family [H]

Homologues:

Organism=Escherichia coli, GI1790135, Length=352, Percent_Identity=37.2159090909091, Blast_Score=267, Evalue=1e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001238
- InterPro:   IPR018078
- InterPro:   IPR003395 [H]

Pfam domain/function: PF02463 SMC_N [H]

EC number: NA

Molecular weight: Translated: 40843; Mature: 40843

Theoretical pI: Translated: 9.63; Mature: 9.63

Prosite motif: PS00618 RECF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.3 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYISNLRLQNFRNIPAKSFDFKNSINFIVGKNGSGKTSILESIYFLSHSRSFRSSQLNRI
CCCCCCCHHHHCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHH
INHNADEFIIYTKAYNPDEITISLSRKKNSNNISKLNLEIQKNHTEITRNLPIQLINPES
HCCCCCEEEEEEECCCCCEEEEEEECCCCCCCCEEEEEEEECCCHHHHHCCCEEEECCCC
FNIINSGAQQRCKVIDWGAFYLDKTFLKIWQQTKFLVKQRNSALKQNYPYSYILSIDKKL
CCEECCCCHHHCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHH
CEFAEILDYKRQAYFTKLKPKIYEILSHFNPNLQLDIDYFRGWNLHKSLAQVLEESFNYD
HHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCEEEEEHHHCCCCHHHHHHHHHHHHCCCC
NKYKVTNHGPHKADIVLSVSHKPIQDIFSRGQQKLLICALKLAQGEIHNSENDNKCIYLI
CCEEECCCCCCCEEEEEEECCCCHHHHHHCCCCEEEHHHHHHHHCCCCCCCCCCEEEEEE
DDITSELDSIHTLTLFNYLKQLKSQVFITTTEKNKINEFIDTNSYILEI
HHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHCCCCEEEEC
>Mature Secondary Structure
MYISNLRLQNFRNIPAKSFDFKNSINFIVGKNGSGKTSILESIYFLSHSRSFRSSQLNRI
CCCCCCCHHHHCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHH
INHNADEFIIYTKAYNPDEITISLSRKKNSNNISKLNLEIQKNHTEITRNLPIQLINPES
HCCCCCEEEEEEECCCCCEEEEEEECCCCCCCCEEEEEEEECCCHHHHHCCCEEEECCCC
FNIINSGAQQRCKVIDWGAFYLDKTFLKIWQQTKFLVKQRNSALKQNYPYSYILSIDKKL
CCEECCCCHHHCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHH
CEFAEILDYKRQAYFTKLKPKIYEILSHFNPNLQLDIDYFRGWNLHKSLAQVLEESFNYD
HHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCEEEEEHHHCCCCHHHHHHHHHHHHCCCC
NKYKVTNHGPHKADIVLSVSHKPIQDIFSRGQQKLLICALKLAQGEIHNSENDNKCIYLI
CCEEECCCCCCCEEEEEEECCCCHHHHHHCCCCEEEHHHHHHHHCCCCCCCCCCEEEEEE
DDITSELDSIHTLTLFNYLKQLKSQVFITTTEKNKINEFIDTNSYILEI
HHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA