Click here to switch to the map view.

The map label for this gene is gshA [H]

Identifier: 89256613

GI number: 89256613

Start: 1241081

End: 1242586

Strand: Reverse

Name: gshA [H]

Synonym: FTL_1304

Alternate gene names: 89256613

Gene position: 1242586-1241081 (Counterclockwise)

Preceding gene: 89256614

Following gene: 89256609

Centisome position: 65.54

GC content: 32.93

Gene sequence:

>1506_bases
ATGTACGATTTCAAAAAAATAAATAACCTACGCGGGATAGAAAGAGAAACTTTAAGAGTTACTGATTGTGGTAATCTAGT
AACTTCTAATCACCCTGATGGTTTAGGACATAAGTTGACTAATAATAGTATTACCGTTGATTTCTCTGAAAATTTGCTTG
AGCTGATAACTAAACCGCATGATAGTATAGATAAAGCAATCGGTGAGCTATATCAACTTTCGGCATTTACTTTGGATAAT
ATGCACAATGATGAAATTATCCTTAATACTAGTATGCCACTATCTGCTAACGATAATGATATACAGGAAGCAGATTTTGG
GAGTTCAAATTCGGGGCGAATGAAGCGTGTTTACCGTAAAGGGCTATCTGCGCGATATGGTAAAATTATGCAGATAATTT
CCGGGATACATTATAATTTTTCTTTTGATAAAGACTTAATTAGTAATATTGCTACTAACAAGCAAGTATCAATATCAGAT
ATTTATTTTGATGTGCTTAATAACTATTTTGAGTTTATGTGGCTTTTACCGTATCTTTTTGGCGCTAGTCCTATATGTGC
TAAGACTTCTGTTAAAAATAAACCTGATTATTTATTAGTTTTAGATGATAAGTTTTATGTTGGTGAATACGCTACAAGCT
TGAGAATGAGTGACCTTGGCTACACAAGTCCCGCACAAAAAGATTTAGCAATATCATATGATAACGTTAAGGCATATGTT
AAAGATTTAATTCAAGCAACTGATGATACTTTTGCTGATTATAAGCGTATAGGCCTTTATAATTCTCAAGGGCAAAGAAT
ACAGTTAAATGATAGTATTTTACAGATAGAAAATGAATATTACAGCGCAATTAGACCTAAGCAAATTGCTAAAAGAGGAG
AGAGACCTGCTTGTGCATTATATAACCGTGGTGTTGAGTATGTTGAGGTTAGAGTTCTTGATGTCGATCCATTTGAGCCG
GTGGGTATAAGCAAAGATACAGCGCTTTTTGTTGAGGCGATGTTGATGACTTGTCTAGACAAGGATGCTAAAAAATATCA
TAAAGATATTATCAAACAGGCAAAACAAAATTTAACTGCAGTTGCAATACAGGGACGTAATCCACAACTTAAGCTTAAAA
AGCTTGATGATGATAGTGAAATACTTTTAAAAGATTATGCTTTAGAATTGTTTGATGAAATTGAGGCTGTAGCTAAGAAA
ATGCCTAAAGAATACTTAGATGCTGTTGAAATTCAAAAGCGCAAAGTATTAGATATATCGCAAACACCTTCAGCTAAAAT
AATAGAATTAGCTAGACAACATGGTTATAAAAAATTTATCTTAGATATATCTCGCCAAGTATCACAACAATTCAGAAGTT
ATGAGCTTCCAGCAGCAATTGTAGCTAAATTAAAAGACCAAGCTGGCCAATCTGTTGCTGCAGAAAAAGAATTAGTTGCT
AATGATAAAATTTCCTTAGATGAATATATCAATAGATATTATGAATCTTCAAAGGGTTGTTGTTAG

Upstream 100 bases:

>100_bases
ACTATACTGCTATTAGTGGGGATCAAGCCGCTAAAAACACTAGAATGATAATTTATTAACTAGTTATAATCATTAGTGAA
AAAACTTATTTAGGATAATA

Downstream 100 bases:

>100_bases
GTGGAGTGTAATGCTGGTAAAAAATTTTACTATAACACCTCATTTAAATATAATCTATCATAAAAATCTCATACTTAATT
TGTTTACTGAGTCAGTCAAA

Product: glutamate--cysteine ligase

Products: NA

Alternate protein names: Gamma-ECS; GCS; Gamma-glutamylcysteine synthetase [H]

Number of amino acids: Translated: 501; Mature: 501

Protein sequence:

>501_residues
MYDFKKINNLRGIERETLRVTDCGNLVTSNHPDGLGHKLTNNSITVDFSENLLELITKPHDSIDKAIGELYQLSAFTLDN
MHNDEIILNTSMPLSANDNDIQEADFGSSNSGRMKRVYRKGLSARYGKIMQIISGIHYNFSFDKDLISNIATNKQVSISD
IYFDVLNNYFEFMWLLPYLFGASPICAKTSVKNKPDYLLVLDDKFYVGEYATSLRMSDLGYTSPAQKDLAISYDNVKAYV
KDLIQATDDTFADYKRIGLYNSQGQRIQLNDSILQIENEYYSAIRPKQIAKRGERPACALYNRGVEYVEVRVLDVDPFEP
VGISKDTALFVEAMLMTCLDKDAKKYHKDIIKQAKQNLTAVAIQGRNPQLKLKKLDDDSEILLKDYALELFDEIEAVAKK
MPKEYLDAVEIQKRKVLDISQTPSAKIIELARQHGYKKFILDISRQVSQQFRSYELPAAIVAKLKDQAGQSVAAEKELVA
NDKISLDEYINRYYESSKGCC

Sequences:

>Translated_501_residues
MYDFKKINNLRGIERETLRVTDCGNLVTSNHPDGLGHKLTNNSITVDFSENLLELITKPHDSIDKAIGELYQLSAFTLDN
MHNDEIILNTSMPLSANDNDIQEADFGSSNSGRMKRVYRKGLSARYGKIMQIISGIHYNFSFDKDLISNIATNKQVSISD
IYFDVLNNYFEFMWLLPYLFGASPICAKTSVKNKPDYLLVLDDKFYVGEYATSLRMSDLGYTSPAQKDLAISYDNVKAYV
KDLIQATDDTFADYKRIGLYNSQGQRIQLNDSILQIENEYYSAIRPKQIAKRGERPACALYNRGVEYVEVRVLDVDPFEP
VGISKDTALFVEAMLMTCLDKDAKKYHKDIIKQAKQNLTAVAIQGRNPQLKLKKLDDDSEILLKDYALELFDEIEAVAKK
MPKEYLDAVEIQKRKVLDISQTPSAKIIELARQHGYKKFILDISRQVSQQFRSYELPAAIVAKLKDQAGQSVAAEKELVA
NDKISLDEYINRYYESSKGCC
>Mature_501_residues
MYDFKKINNLRGIERETLRVTDCGNLVTSNHPDGLGHKLTNNSITVDFSENLLELITKPHDSIDKAIGELYQLSAFTLDN
MHNDEIILNTSMPLSANDNDIQEADFGSSNSGRMKRVYRKGLSARYGKIMQIISGIHYNFSFDKDLISNIATNKQVSISD
IYFDVLNNYFEFMWLLPYLFGASPICAKTSVKNKPDYLLVLDDKFYVGEYATSLRMSDLGYTSPAQKDLAISYDNVKAYV
KDLIQATDDTFADYKRIGLYNSQGQRIQLNDSILQIENEYYSAIRPKQIAKRGERPACALYNRGVEYVEVRVLDVDPFEP
VGISKDTALFVEAMLMTCLDKDAKKYHKDIIKQAKQNLTAVAIQGRNPQLKLKKLDDDSEILLKDYALELFDEIEAVAKK
MPKEYLDAVEIQKRKVLDISQTPSAKIIELARQHGYKKFILDISRQVSQQFRSYELPAAIVAKLKDQAGQSVAAEKELVA
NDKISLDEYINRYYESSKGCC

Specific function: Glutathione biosynthesis; first step. [C]

COG id: COG2918

COG function: function code H; Gamma-glutamylcysteine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamate--cysteine ligase type 1 family. Type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789044, Length=399, Percent_Identity=39.8496240601504, Blast_Score=297, Evalue=9e-82,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007370
- InterPro:   IPR006334 [H]

Pfam domain/function: PF04262 Glu_cys_ligase [H]

EC number: =6.3.2.2 [H]

Molecular weight: Translated: 56964; Mature: 56964

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYDFKKINNLRGIERETLRVTDCGNLVTSNHPDGLGHKLTNNSITVDFSENLLELITKPH
CCCHHHHHCCCCCCHHEEEEECCCCCCCCCCCCCCCCEECCCCEEEEHHHHHHHHHHCCC
DSIDKAIGELYQLSAFTLDNMHNDEIILNTSMPLSANDNDIQEADFGSSNSGRMKRVYRK
HHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
GLSARYGKIMQIISGIHYNFSFDKDLISNIATNKQVSISDIYFDVLNNYFEFMWLLPYLF
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHH
GASPICAKTSVKNKPDYLLVLDDKFYVGEYATSLRMSDLGYTSPAQKDLAISYDNVKAYV
CCCCCEECCCCCCCCCEEEEECCCEEHHHHHHHHHHHCCCCCCCCCCCCEEEHHHHHHHH
KDLIQATDDTFADYKRIGLYNSQGQRIQLNDSILQIENEYYSAIRPKQIAKRGERPACAL
HHHHHHCCCHHHHHHHHCCCCCCCCEEEECCHHEEECHHHHHHCCHHHHHHCCCCCCHHH
YNRGVEYVEVRVLDVDPFEPVGISKDTALFVEAMLMTCLDKDAKKYHKDIIKQAKQNLTA
HHCCCEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCEE
VAIQGRNPQLKLKKLDDDSEILLKDYALELFDEIEAVAKKMPKEYLDAVEIQKRKVLDIS
EEEECCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHEECCC
QTPSAKIIELARQHGYKKFILDISRQVSQQFRSYELPAAIVAKLKDQAGQSVAAEKELVA
CCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHC
NDKISLDEYINRYYESSKGCC
CCCCCHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MYDFKKINNLRGIERETLRVTDCGNLVTSNHPDGLGHKLTNNSITVDFSENLLELITKPH
CCCHHHHHCCCCCCHHEEEEECCCCCCCCCCCCCCCCEECCCCEEEEHHHHHHHHHHCCC
DSIDKAIGELYQLSAFTLDNMHNDEIILNTSMPLSANDNDIQEADFGSSNSGRMKRVYRK
HHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
GLSARYGKIMQIISGIHYNFSFDKDLISNIATNKQVSISDIYFDVLNNYFEFMWLLPYLF
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHH
GASPICAKTSVKNKPDYLLVLDDKFYVGEYATSLRMSDLGYTSPAQKDLAISYDNVKAYV
CCCCCEECCCCCCCCCEEEEECCCEEHHHHHHHHHHHCCCCCCCCCCCCEEEHHHHHHHH
KDLIQATDDTFADYKRIGLYNSQGQRIQLNDSILQIENEYYSAIRPKQIAKRGERPACAL
HHHHHHCCCHHHHHHHHCCCCCCCCEEEECCHHEEECHHHHHHCCHHHHHHCCCCCCHHH
YNRGVEYVEVRVLDVDPFEPVGISKDTALFVEAMLMTCLDKDAKKYHKDIIKQAKQNLTA
HHCCCEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCEE
VAIQGRNPQLKLKKLDDDSEILLKDYALELFDEIEAVAKKMPKEYLDAVEIQKRKVLDIS
EEEECCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHEECCC
QTPSAKIIELARQHGYKKFILDISRQVSQQFRSYELPAAIVAKLKDQAGQSVAAEKELVA
CCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHC
NDKISLDEYINRYYESSKGCC
CCCCCHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA