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The map label for this gene is folE [H]

Identifier: 89256571

GI number: 89256571

Start: 1198254

End: 1198871

Strand: Direct

Name: folE [H]

Synonym: FTL_1253

Alternate gene names: 89256571

Gene position: 1198254-1198871 (Clockwise)

Preceding gene: 89256569

Following gene: 89256572

Centisome position: 63.2

GC content: 32.04

Gene sequence:

>618_bases
ATGATGAAAGATAAATATTGCTCTAAATTAGGTAAAAGTGTCCAAGAACATCTCATCAAGTTAGGTTTAGAGCAACCTAG
AGAATTTAACCTTGATAATGATAATAAAATAACCATAATCACCAAAGCATACCGACAAATCTTAGATGCTCTCGGCTTAT
ACACAGATGAATTTGAAAAAACTCCATTTAGAGTAGCGAGGATGTTTACTCAAGAAATATTTAATGGACTTGATTATGCT
AATTTCCCTGCTTGTGCTTTATACGAGAACGAGTTTAACTATACTGGTGTGCTAACTCAAAAAAATATAACTATAATGTC
ATTTTGTGAGCACCATTTTGTACCTTTTGAAGGTACTGCAGAAGTTTCTTTTATTCCTAAAAATAATAATATTATAGGGC
TTTGTCGTATAAATAGCATTTGTGACTTTTTTTCCAGACGCCCACAAATACAAGAAAGAATGACCGCTCAAATATTTGAG
GCCTTAAAATTCATTTTATCGACAGAAGATGTTAGTGTGAAAATTAAGGCAAAACATGCTTGTGTCTCACTTAGAGGGGT
AAATAATCAAAATTCTCAAACATATACACAAATGGTTGGTGGAGTTTTTGCTAAATAA

Upstream 100 bases:

>100_bases
TAGCTATATTAACACTAATAGCTAGGATTTACTCTATAAAGATGATTTATTAAATAATATAATACATTCTAATAAATTTA
GTTATAGTATAATAAATTTG

Downstream 100 bases:

>100_bases
AAATGATATTAAGATGAGTAGTTTATTAGATATTGGATGTTTGTTTTTAATTGCCTTGATTATTACTTGTATATTTTCAA
AAGGTTATTTGATATATTTT

Product: GTP cyclohydrolase I

Products: NA

Alternate protein names: GTP cyclohydrolase I; GTP-CH-I [H]

Number of amino acids: Translated: 205; Mature: 205

Protein sequence:

>205_residues
MMKDKYCSKLGKSVQEHLIKLGLEQPREFNLDNDNKITIITKAYRQILDALGLYTDEFEKTPFRVARMFTQEIFNGLDYA
NFPACALYENEFNYTGVLTQKNITIMSFCEHHFVPFEGTAEVSFIPKNNNIIGLCRINSICDFFSRRPQIQERMTAQIFE
ALKFILSTEDVSVKIKAKHACVSLRGVNNQNSQTYTQMVGGVFAK

Sequences:

>Translated_205_residues
MMKDKYCSKLGKSVQEHLIKLGLEQPREFNLDNDNKITIITKAYRQILDALGLYTDEFEKTPFRVARMFTQEIFNGLDYA
NFPACALYENEFNYTGVLTQKNITIMSFCEHHFVPFEGTAEVSFIPKNNNIIGLCRINSICDFFSRRPQIQERMTAQIFE
ALKFILSTEDVSVKIKAKHACVSLRGVNNQNSQTYTQMVGGVFAK
>Mature_205_residues
MMKDKYCSKLGKSVQEHLIKLGLEQPREFNLDNDNKITIITKAYRQILDALGLYTDEFEKTPFRVARMFTQEIFNGLDYA
NFPACALYENEFNYTGVLTQKNITIMSFCEHHFVPFEGTAEVSFIPKNNNIIGLCRINSICDFFSRRPQIQERMTAQIFE
ALKFILSTEDVSVKIKAKHACVSLRGVNNQNSQTYTQMVGGVFAK

Specific function: Tetrahydrofolate biosynthesis; first step. [C]

COG id: COG0302

COG function: function code H; GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family [H]

Homologues:

Organism=Homo sapiens, GI66932968, Length=189, Percent_Identity=33.3333333333333, Blast_Score=102, Evalue=2e-22,
Organism=Homo sapiens, GI4503949, Length=189, Percent_Identity=33.3333333333333, Blast_Score=102, Evalue=2e-22,
Organism=Homo sapiens, GI66932972, Length=166, Percent_Identity=31.3253012048193, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI66932970, Length=163, Percent_Identity=31.2883435582822, Blast_Score=78, Evalue=5e-15,
Organism=Escherichia coli, GI1788476, Length=201, Percent_Identity=43.2835820895522, Blast_Score=167, Evalue=6e-43,
Organism=Caenorhabditis elegans, GI17560486, Length=184, Percent_Identity=32.0652173913043, Blast_Score=97, Evalue=6e-21,
Organism=Saccharomyces cerevisiae, GI6321706, Length=115, Percent_Identity=39.1304347826087, Blast_Score=90, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24656782, Length=176, Percent_Identity=34.0909090909091, Blast_Score=92, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24656796, Length=176, Percent_Identity=34.0909090909091, Blast_Score=91, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24656791, Length=176, Percent_Identity=34.0909090909091, Blast_Score=91, Evalue=4e-19,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001474
- InterPro:   IPR020602
- InterPro:   IPR018234 [H]

Pfam domain/function: PF01227 GTP_cyclohydroI [H]

EC number: =3.5.4.16 [H]

Molecular weight: Translated: 23550; Mature: 23550

Theoretical pI: Translated: 8.11; Mature: 8.11

Prosite motif: PS00859 GTP_CYCLOHYDROL_1_1 ; PS00860 GTP_CYCLOHYDROL_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKDKYCSKLGKSVQEHLIKLGLEQPREFNLDNDNKITIITKAYRQILDALGLYTDEFEK
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHCCCHHHHCC
TPFRVARMFTQEIFNGLDYANFPACALYENEFNYTGVLTQKNITIMSFCEHHFVPFEGTA
CHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCEEEEEEECCCHHEEHHHHCCCCCCCCC
EVSFIPKNNNIIGLCRINSICDFFSRRPQIQERMTAQIFEALKFILSTEDVSVKIKAKHA
EEEEEECCCCEEEEEEHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEE
CVSLRGVNNQNSQTYTQMVGGVFAK
EEEEECCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MMKDKYCSKLGKSVQEHLIKLGLEQPREFNLDNDNKITIITKAYRQILDALGLYTDEFEK
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHCCCHHHHCC
TPFRVARMFTQEIFNGLDYANFPACALYENEFNYTGVLTQKNITIMSFCEHHFVPFEGTA
CHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCEEEEEEECCCHHEEHHHHCCCCCCCCC
EVSFIPKNNNIIGLCRINSICDFFSRRPQIQERMTAQIFEALKFILSTEDVSVKIKAKHA
EEEEEECCCCEEEEEEHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEE
CVSLRGVNNQNSQTYTQMVGGVFAK
EEEEECCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]