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The map label for this gene is rsmB [H]

Identifier: 89256456

GI number: 89256456

Start: 1074770

End: 1075246

Strand: Reverse

Name: rsmB [H]

Synonym: FTL_1131

Alternate gene names: 89256456

Gene position: 1075246-1074770 (Counterclockwise)

Preceding gene: 89256457

Following gene: 89256455

Centisome position: 56.71

GC content: 29.77

Gene sequence:

>477_bases
ATGAATACCCGCGCTATTGCAGCTAAAACTATTTTAGATATTCTCGATAACAAATATTCTCTACTAACTATTGAGCAAAA
GCTTGTTAAGCTTAACCTATTAGAGCAAGATAAATCTTTTATAAAACTCTTATGTTATGAATTTTTCAGACATTACTACT
CGTTAGAAAAGATTATTGCTTTATATCTGACACAAAAAACTAAGGTAAAAGCTAAAGTTTTAATAATGCTTGGAGCACTA
CATATATTTGAAATCAAACAACCTTACTATGCAACTATTAATGAAACTGTAGCAGCTTGTAAAGTTCTCAAAATTATCTG
GGCAAAGAAACTAGTTAATCGAATACTAAGAGAAATGACTCGTAATATCGAAGATATAACTTCTATCTATACTCAAAATA
AAGCAATAGATATGCCTGAATGGCTTTGTGATACTCTCAAAAAGCAATATCCTAACGATCATCTAGTGATAGCTTAA

Upstream 100 bases:

>100_bases
ACATCCATGGTACTAGTGACCTTGACTCTGGTTTGATCGTTGCTGGTAATAAAAAAATCCAACCAAAACTTATTAAAATT
CTAGCAAAACATATCAAATA

Downstream 100 bases:

>100_bases
GCAATCAATGCAAAAGCTGATATTTTTATTCGTTTAAACCAAAACAAAGATAATAAAAAAGTCTTAGATTATTTTGACAA
AAATAACATAGGATATTCTT

Product: Sun protein

Products: NA

Alternate protein names: 16S rRNA m5C967 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase rsmB [H]

Number of amino acids: Translated: 158; Mature: 158

Protein sequence:

>158_residues
MNTRAIAAKTILDILDNKYSLLTIEQKLVKLNLLEQDKSFIKLLCYEFFRHYYSLEKIIALYLTQKTKVKAKVLIMLGAL
HIFEIKQPYYATINETVAACKVLKIIWAKKLVNRILREMTRNIEDITSIYTQNKAIDMPEWLCDTLKKQYPNDHLVIA

Sequences:

>Translated_158_residues
MNTRAIAAKTILDILDNKYSLLTIEQKLVKLNLLEQDKSFIKLLCYEFFRHYYSLEKIIALYLTQKTKVKAKVLIMLGAL
HIFEIKQPYYATINETVAACKVLKIIWAKKLVNRILREMTRNIEDITSIYTQNKAIDMPEWLCDTLKKQYPNDHLVIA
>Mature_158_residues
MNTRAIAAKTILDILDNKYSLLTIEQKLVKLNLLEQDKSFIKLLCYEFFRHYYSLEKIIALYLTQKTKVKAKVLIMLGAL
HIFEIKQPYYATINETVAACKVLKIIWAKKLVNRILREMTRNIEDITSIYTQNKAIDMPEWLCDTLKKQYPNDHLVIA

Specific function: Specifically methylates the cytosine at position 967 (m5C967) of 16S rRNA [H]

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Escherichia coli, GI2367212, Length=155, Percent_Identity=27.741935483871, Blast_Score=62, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR018314
- InterPro:   IPR006027
- InterPro:   IPR004573 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu; PF01029 NusB [H]

EC number: =2.1.1.176 [H]

Molecular weight: Translated: 18554; Mature: 18554

Theoretical pI: Translated: 9.74; Mature: 9.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTRAIAAKTILDILDNKYSLLTIEQKLVKLNLLEQDKSFIKLLCYEFFRHYYSLEKIIA
CCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LYLTQKTKVKAKVLIMLGALHIFEIKQPYYATINETVAACKVLKIIWAKKLVNRILREMT
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RNIEDITSIYTQNKAIDMPEWLCDTLKKQYPNDHLVIA
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCEEEC
>Mature Secondary Structure
MNTRAIAAKTILDILDNKYSLLTIEQKLVKLNLLEQDKSFIKLLCYEFFRHYYSLEKIIA
CCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LYLTQKTKVKAKVLIMLGALHIFEIKQPYYATINETVAACKVLKIIWAKKLVNRILREMT
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RNIEDITSIYTQNKAIDMPEWLCDTLKKQYPNDHLVIA
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]