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The map label for this gene is rsmB [H]

Identifier: 89256455

GI number: 89256455

Start: 1073966

End: 1074358

Strand: Reverse

Name: rsmB [H]

Synonym: FTL_1130

Alternate gene names: 89256455

Gene position: 1074358-1073966 (Counterclockwise)

Preceding gene: 89256456

Following gene: 89256451

Centisome position: 56.66

GC content: 30.79

Gene sequence:

>393_bases
TTGACTCTACCTCTAGCCGGAAAATTTAATAAGATTATCCTTGATGCGCCATGCTCAGCCTTAGGAACTATAAGACGAAA
TCCCGACATCAAAGTGCTAAGAACACCGAATGATATTCAAGCTATCAAAAGCTTACAAAGTAAAATTTTGGCTAACTTAT
GGAATAATAACCTTACTGATAATGGTTACTTACTCTATACTACATGTTCAATACTAGCAGAAGAAAATCAACAACAAATA
AAAGAGTTTTTAGCTAAAAATAATAATGCTAAGATTATTGAGATAGATATCCTTGAAAAATATAAAACTAGTTATGGCTA
TCAATTACTGCCATCTCAACAAGATGGTGATGGCTTTTATTACTGTTTACTTAAAAAAAATCCTTATTTTTAG

Upstream 100 bases:

>100_bases
TATTACTGCTATAGATATTATTGATAAGCGTTTAGAACTTCTTAGAGAAAACTTAGAGAGATTATCTGAAAATCATAATG
TCAATGTTTTTAAACATGAT

Downstream 100 bases:

>100_bases
CAGCAATTTTTTCTATAATTTCTTGAGGTGTTGCAGAAAGTTTTAACTTATTTATATGGTACTGCGAGGAGAAAATAATA
TCATCTATTTTATCTTCAAT

Product: ribosomal RNA small subunit methyltransferase

Products: NA

Alternate protein names: 16S rRNA m5C967 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase rsmB [H]

Number of amino acids: Translated: 130; Mature: 129

Protein sequence:

>130_residues
MTLPLAGKFNKIILDAPCSALGTIRRNPDIKVLRTPNDIQAIKSLQSKILANLWNNNLTDNGYLLYTTCSILAEENQQQI
KEFLAKNNNAKIIEIDILEKYKTSYGYQLLPSQQDGDGFYYCLLKKNPYF

Sequences:

>Translated_130_residues
MTLPLAGKFNKIILDAPCSALGTIRRNPDIKVLRTPNDIQAIKSLQSKILANLWNNNLTDNGYLLYTTCSILAEENQQQI
KEFLAKNNNAKIIEIDILEKYKTSYGYQLLPSQQDGDGFYYCLLKKNPYF
>Mature_129_residues
TLPLAGKFNKIILDAPCSALGTIRRNPDIKVLRTPNDIQAIKSLQSKILANLWNNNLTDNGYLLYTTCSILAEENQQQIK
EFLAKNNNAKIIEIDILEKYKTSYGYQLLPSQQDGDGFYYCLLKKNPYF

Specific function: Specifically methylates the cytosine at position 967 (m5C967) of 16S rRNA [H]

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Escherichia coli, GI2367212, Length=120, Percent_Identity=48.3333333333333, Blast_Score=108, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17536757, Length=134, Percent_Identity=28.3582089552239, Blast_Score=63, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6324268, Length=120, Percent_Identity=31.6666666666667, Blast_Score=65, Evalue=3e-12,
Organism=Drosophila melanogaster, GI22024126, Length=134, Percent_Identity=29.1044776119403, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR018314
- InterPro:   IPR006027
- InterPro:   IPR004573 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu; PF01029 NusB [H]

EC number: =2.1.1.176 [H]

Molecular weight: Translated: 14836; Mature: 14705

Theoretical pI: Translated: 8.76; Mature: 8.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLPLAGKFNKIILDAPCSALGTIRRNPDIKVLRTPNDIQAIKSLQSKILANLWNNNLTD
CCCCCCCCCCEEEEECCCHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCCC
NGYLLYTTCSILAEENQQQIKEFLAKNNNAKIIEIDILEKYKTSYGYQLLPSQQDGDGFY
CCEEEEEEEEHHHCCCHHHHHHHHHCCCCCEEEEEEEEHHHHHCCCEEECCCCCCCCEEE
YCLLKKNPYF
EEEEECCCCC
>Mature Secondary Structure 
TLPLAGKFNKIILDAPCSALGTIRRNPDIKVLRTPNDIQAIKSLQSKILANLWNNNLTD
CCCCCCCCCEEEEECCCHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCCC
NGYLLYTTCSILAEENQQQIKEFLAKNNNAKIIEIDILEKYKTSYGYQLLPSQQDGDGFY
CCEEEEEEEEHHHCCCHHHHHHHHHCCCCCEEEEEEEEHHHHHCCCEEECCCCCCCCEEE
YCLLKKNPYF
EEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100 [H]