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The map label for this gene is rep [H]

Identifier: 89256441

GI number: 89256441

Start: 1060774

End: 1062789

Strand: Direct

Name: rep [H]

Synonym: FTL_1116

Alternate gene names: 89256441

Gene position: 1060774-1062789 (Clockwise)

Preceding gene: 89256440

Following gene: 89256442

Centisome position: 55.95

GC content: 31.45

Gene sequence:

>2016_bases
ATGCTAGATAATCTTAATCAACAGCAGCAACAAGCTGTCAAGTATATAGCAACACCATTACTTGTATTAGCAGGTGCAGG
TAGTGGTAAAACTAGTGTAATTATAGAGAAAATTTCTTATCTTATTGAACAGCTTTTATATCCTGCAAAAAGCATTTTAG
CGGTGACTTTTACTAATAAAGCAGCTAAAGAAATGCAAGAGCGTGTCAAATCGCGCCTTGATAAAGAAAAGTCAAAAGGC
TTGATGATATCTACTTTTCACTCGCTTGGTTTATCAATTCTGAAAAAGCATTTTAACGAGCTAGGATATAAGAAGAATTT
TACGCTATTTGATAGTCATGATTCTTTAGCTTTAATATATGATATTGCCTATGATGAATATCAGCTACCTAAGCAGAATG
CTAGTTTTATCCAAGCAAAAATATCATTTTGGAAGTCTGCTTTACTTACTCCTGATCAAGTTAAGCCTAAAGATGAATTA
GAAGAGCAGGCAGCTTTCATTTACCAAGAATATCAAAAATACCTAAAATCATATAACTCTTTTGATTTTGATGATCTAAT
ATTTCAACCAATACAGTTGTTTAGGAATCATTCTAATATCCAGAAATTTTGGGCAGATAAGTTTAGGTATATATTGATAG
ATGAATATCAAGATACTAATGAATCGCAATATCAGCTTTTAAAATATTTAACTCTAGGAAAAAATAAATTTACTGTAGTA
GGTGATGATGATCAATCTATATACGCATGGCGTGGTTCAAGACCAGAAAATTTACGCCATTTACAAGAAGATTTTGCTGA
TCTAAAGGTTATCAAATTAGAGCAGAATTATCGTTCTACAGGTAGAATTTTAAATGTTGCTAATAAGCTTATAGAAAATA
ATAGCCATATTTTTGACAAAAAACTTTGGTCTAATAAAGACTATGGTGAGCAGATTAAGGTAATTAGTTTGATAAATGAT
GAGGATGAGGCACAATTTATAGCTAGTGATATATTTTTTGATAGAGTTAAGACGAAATCAAAAAATTCTGATTATGCAGT
ACTAATTAGGAGTAATTATCAAGCATATCTACTTGAAAGATATATGCAAATACACAAAATTCCATATACTATCAGTGGTG
GCAGCTCATTTTTTTCTAAAGCCGAAATCAAAGATATTATTTCGTATTTGAGATTAATTGTTAATCCTGATGATGATAGA
GCTTTTTTACGCGTGATAAATACACCTAAACGAGAGGTTGGTAGTGCGACTGTTCACAAGTTAGGAGAGTATGCAAGCCA
GCATCATTGTAGTTTTTTTCATACATTGTATAGTTTAGAAAATTTTGAATTACGTGATTTTACTAAGAGAAATCTAATAT
CTTTTAAGGATTTAATACTCAACACTCAACAGCAGATAAACACAAGTATCTCAGTACAAGAGTTAAAAAATATAATTAAT
AGTTTTATTGATAATATTTCATATAGACAATGGTTAATAGACTCTAGTTCTTCTGAAAAGCAAGCTGAGTTTAGGTATGC
AAATATTATTGAAGTTACGAAATGGATTGTAAACCAACTTGAGGATGAATCATATAATGGGCTTGAGTCATTAGCAACCG
TATTGAATAAAATGCTATTGATAGATATTTTAGATAGAGATAATGAAGATAAAAATGACAATCAAGTTCAGATTATAACT
ATGCATGCCTCGAAAGGTCTAGAATTTAAAAAAGTCTATATCATGGGAATGGAAGAGGGGATATTACCACATCAACAAAG
TATTGAGGATGATTCAATAGAAGATGAGCGTCGCCTTGCCTATGTCGCGATTACTCGAGCTAGAGAGAATCTCACCATCA
CGATGACAAAACATCGTAAAAAATTTGGTGAAAAACAAGTTTCAGTTCCAAGTAGATTTATTGATGAGTTACCAGAGACA
GATCTATACTGGGTTGGCACAGAAAAAGAATGTGCAGAAACCCGTAAGCAAAATTCAAAACAAAATATTTCAGCATTAAA
GGATATGTTTGGCTAG

Upstream 100 bases:

>100_bases
ATAAAGTATTTGTTGATAACCAGTGCTATATGCTAGTTGTGTTTCATAAAGAAGCTTCTGAGCAAGAGTATCAATTAGAT
GATGGCAAAATTATAACTAA

Downstream 100 bases:

>100_bases
TTTAAGCAAATATATGGTATGATTTACCTGCATAAAAATATCAAAGGTAATTATTTTGATTTCAGTTGGAAAATATCACA
AATTAGAAGTTCTCGATAAA

Product: ATP-dependent DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 671; Mature: 671

Protein sequence:

>671_residues
MLDNLNQQQQQAVKYIATPLLVLAGAGSGKTSVIIEKISYLIEQLLYPAKSILAVTFTNKAAKEMQERVKSRLDKEKSKG
LMISTFHSLGLSILKKHFNELGYKKNFTLFDSHDSLALIYDIAYDEYQLPKQNASFIQAKISFWKSALLTPDQVKPKDEL
EEQAAFIYQEYQKYLKSYNSFDFDDLIFQPIQLFRNHSNIQKFWADKFRYILIDEYQDTNESQYQLLKYLTLGKNKFTVV
GDDDQSIYAWRGSRPENLRHLQEDFADLKVIKLEQNYRSTGRILNVANKLIENNSHIFDKKLWSNKDYGEQIKVISLIND
EDEAQFIASDIFFDRVKTKSKNSDYAVLIRSNYQAYLLERYMQIHKIPYTISGGSSFFSKAEIKDIISYLRLIVNPDDDR
AFLRVINTPKREVGSATVHKLGEYASQHHCSFFHTLYSLENFELRDFTKRNLISFKDLILNTQQQINTSISVQELKNIIN
SFIDNISYRQWLIDSSSSEKQAEFRYANIIEVTKWIVNQLEDESYNGLESLATVLNKMLLIDILDRDNEDKNDNQVQIIT
MHASKGLEFKKVYIMGMEEGILPHQQSIEDDSIEDERRLAYVAITRARENLTITMTKHRKKFGEKQVSVPSRFIDELPET
DLYWVGTEKECAETRKQNSKQNISALKDMFG

Sequences:

>Translated_671_residues
MLDNLNQQQQQAVKYIATPLLVLAGAGSGKTSVIIEKISYLIEQLLYPAKSILAVTFTNKAAKEMQERVKSRLDKEKSKG
LMISTFHSLGLSILKKHFNELGYKKNFTLFDSHDSLALIYDIAYDEYQLPKQNASFIQAKISFWKSALLTPDQVKPKDEL
EEQAAFIYQEYQKYLKSYNSFDFDDLIFQPIQLFRNHSNIQKFWADKFRYILIDEYQDTNESQYQLLKYLTLGKNKFTVV
GDDDQSIYAWRGSRPENLRHLQEDFADLKVIKLEQNYRSTGRILNVANKLIENNSHIFDKKLWSNKDYGEQIKVISLIND
EDEAQFIASDIFFDRVKTKSKNSDYAVLIRSNYQAYLLERYMQIHKIPYTISGGSSFFSKAEIKDIISYLRLIVNPDDDR
AFLRVINTPKREVGSATVHKLGEYASQHHCSFFHTLYSLENFELRDFTKRNLISFKDLILNTQQQINTSISVQELKNIIN
SFIDNISYRQWLIDSSSSEKQAEFRYANIIEVTKWIVNQLEDESYNGLESLATVLNKMLLIDILDRDNEDKNDNQVQIIT
MHASKGLEFKKVYIMGMEEGILPHQQSIEDDSIEDERRLAYVAITRARENLTITMTKHRKKFGEKQVSVPSRFIDELPET
DLYWVGTEKECAETRKQNSKQNISALKDMFG
>Mature_671_residues
MLDNLNQQQQQAVKYIATPLLVLAGAGSGKTSVIIEKISYLIEQLLYPAKSILAVTFTNKAAKEMQERVKSRLDKEKSKG
LMISTFHSLGLSILKKHFNELGYKKNFTLFDSHDSLALIYDIAYDEYQLPKQNASFIQAKISFWKSALLTPDQVKPKDEL
EEQAAFIYQEYQKYLKSYNSFDFDDLIFQPIQLFRNHSNIQKFWADKFRYILIDEYQDTNESQYQLLKYLTLGKNKFTVV
GDDDQSIYAWRGSRPENLRHLQEDFADLKVIKLEQNYRSTGRILNVANKLIENNSHIFDKKLWSNKDYGEQIKVISLIND
EDEAQFIASDIFFDRVKTKSKNSDYAVLIRSNYQAYLLERYMQIHKIPYTISGGSSFFSKAEIKDIISYLRLIVNPDDDR
AFLRVINTPKREVGSATVHKLGEYASQHHCSFFHTLYSLENFELRDFTKRNLISFKDLILNTQQQINTSISVQELKNIIN
SFIDNISYRQWLIDSSSSEKQAEFRYANIIEVTKWIVNQLEDESYNGLESLATVLNKMLLIDILDRDNEDKNDNQVQIIT
MHASKGLEFKKVYIMGMEEGILPHQQSIEDDSIEDERRLAYVAITRARENLTITMTKHRKKFGEKQVSVPSRFIDELPET
DLYWVGTEKECAETRKQNSKQNISALKDMFG

Specific function: Rep helicase is a single-stranded DNA-dependent ATPase involved in DNA replication; it can initiate unwinding at a nick in the DNA. It binds to the single-stranded DNA and acts in a progressive fashion along the DNA in the 3' to 5' direction [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI48994965, Length=673, Percent_Identity=45.7652303120357, Blast_Score=593, Evalue=1e-171,
Organism=Escherichia coli, GI2367296, Length=653, Percent_Identity=36.906584992343, Blast_Score=386, Evalue=1e-108,
Organism=Escherichia coli, GI1787196, Length=426, Percent_Identity=25.5868544600939, Blast_Score=95, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6322369, Length=461, Percent_Identity=29.2841648590022, Blast_Score=162, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6324477, Length=687, Percent_Identity=22.5618631732169, Blast_Score=95, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005752
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 78189; Mature: 78189

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDNLNQQQQQAVKYIATPLLVLAGAGSGKTSVIIEKISYLIEQLLYPAKSILAVTFTNK
CCCCCCHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHCHHHEEEEEECCH
AAKEMQERVKSRLDKEKSKGLMISTFHSLGLSILKKHFNELGYKKNFTLFDSHDSLALIY
HHHHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCEEEEE
DIAYDEYQLPKQNASFIQAKISFWKSALLTPDQVKPKDELEEQAAFIYQEYQKYLKSYNS
EECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
FDFDDLIFQPIQLFRNHSNIQKFWADKFRYILIDEYQDTNESQYQLLKYLTLGKNKFTVV
CCHHHHHHHHHHHHHCCHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHCCCCEEEEE
GDDDQSIYAWRGSRPENLRHLQEDFADLKVIKLEQNYRSTGRILNVANKLIENNSHIFDK
CCCCCEEEEECCCCCHHHHHHHHHHHCEEEEEEECCCHHHHHHHHHHHHHHCCCCCEEHH
KLWSNKDYGEQIKVISLINDEDEAQFIASDIFFDRVKTKSKNSDYAVLIRSNYQAYLLER
HHCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHH
YMQIHKIPYTISGGSSFFSKAEIKDIISYLRLIVNPDDDRAFLRVINTPKREVGSATVHK
HHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHH
LGEYASQHHCSFFHTLYSLENFELRDFTKRNLISFKDLILNTQQQINTSISVQELKNIIN
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHCCCCCHHHHHHHHH
SFIDNISYRQWLIDSSSSEKQAEFRYANIIEVTKWIVNQLEDESYNGLESLATVLNKMLL
HHHHCCCHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
IDILDRDNEDKNDNQVQIITMHASKGLEFKKVYIMGMEEGILPHQQSIEDDSIEDERRLA
HHHHCCCCCCCCCCEEEEEEEECCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCHHHHEE
YVAITRARENLTITMTKHRKKFGEKQVSVPSRFIDELPETDLYWVGTEKECAETRKQNSK
EEEEEECCCCEEEEEEHHHHHHCCHHCCCHHHHHHHCCCCCEEEECCCHHHHHHHHCCCH
QNISALKDMFG
HHHHHHHHHCC
>Mature Secondary Structure
MLDNLNQQQQQAVKYIATPLLVLAGAGSGKTSVIIEKISYLIEQLLYPAKSILAVTFTNK
CCCCCCHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHCHHHEEEEEECCH
AAKEMQERVKSRLDKEKSKGLMISTFHSLGLSILKKHFNELGYKKNFTLFDSHDSLALIY
HHHHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCEEEEE
DIAYDEYQLPKQNASFIQAKISFWKSALLTPDQVKPKDELEEQAAFIYQEYQKYLKSYNS
EECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
FDFDDLIFQPIQLFRNHSNIQKFWADKFRYILIDEYQDTNESQYQLLKYLTLGKNKFTVV
CCHHHHHHHHHHHHHCCHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHCCCCEEEEE
GDDDQSIYAWRGSRPENLRHLQEDFADLKVIKLEQNYRSTGRILNVANKLIENNSHIFDK
CCCCCEEEEECCCCCHHHHHHHHHHHCEEEEEEECCCHHHHHHHHHHHHHHCCCCCEEHH
KLWSNKDYGEQIKVISLINDEDEAQFIASDIFFDRVKTKSKNSDYAVLIRSNYQAYLLER
HHCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHH
YMQIHKIPYTISGGSSFFSKAEIKDIISYLRLIVNPDDDRAFLRVINTPKREVGSATVHK
HHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHH
LGEYASQHHCSFFHTLYSLENFELRDFTKRNLISFKDLILNTQQQINTSISVQELKNIIN
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHCCCCCHHHHHHHHH
SFIDNISYRQWLIDSSSSEKQAEFRYANIIEVTKWIVNQLEDESYNGLESLATVLNKMLL
HHHHCCCHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
IDILDRDNEDKNDNQVQIITMHASKGLEFKKVYIMGMEEGILPHQQSIEDDSIEDERRLA
HHHHCCCCCCCCCCEEEEEEEECCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCHHHHEE
YVAITRARENLTITMTKHRKKFGEKQVSVPSRFIDELPETDLYWVGTEKECAETRKQNSK
EEEEEECCCCEEEEEEHHHHHHCCHHCCCHHHHHHHCCCCCEEEECCCHHHHHHHHCCCH
QNISALKDMFG
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]