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The map label for this gene is truA [H]

Identifier: 89256403

GI number: 89256403

Start: 1021016

End: 1021792

Strand: Direct

Name: truA [H]

Synonym: FTL_1068

Alternate gene names: 89256403

Gene position: 1021016-1021792 (Clockwise)

Preceding gene: 89256402

Following gene: 89256404

Centisome position: 53.85

GC content: 33.2

Gene sequence:

>777_bases
ATGAAAAACTACCTACTACAAATCGAATACTTTGGCAAAAACTACTGTGGTTGGCAAAGACAATCTCATTCACTAAGTGT
GCAAGAAGAGCTTGAAAAAGCACTTTCAAAAATAGCAAATCAAAATATAGAAGTTACTTGTGCAGGGCGAACTGATACAG
GCGTGCATGCAACTTCACAGATTGTTAATTTTTATTCTAATGCATATAGGCCTTTAAGCGCATGGCAGCGTGGGGTAAAT
GCTTTGCTACCTCAAGATATTAAAATATTAGCAGTTCAACAAGTTGATAATAATTTTAATTCTAGGTTTACAGCTATAAA
TAGAACTTACAATTATATTATCTACAATTCTGCAACTAGCTCACCAATATTCGCGGAACATTGCTTATGGGAGAATAGAG
AACTTGATATAGATAAGATGAATCAAGCTTGTGAATATCTTCTTGGCGAACAAGATTTTAGTTCTTTTAGATCATCACAA
TGTCAGTCAAATACACCATTTAGAAATATCCAAAAAGCTGAGTTTATCAAGCAGGGTAGTTTTATTGTTTTTGAGGTTGT
TGGTAATGCTTTTTTACATCATATGATTAGAAATTTAGTAGGCTCATTATTAAAAGTAGGCTTAGGTTTTGAATCTCCAG
AATGGATTAAAGTAGTATTAGAAGCTAAAGATAGAACACAAGCAGCTGAGACAGCTAAAGCACATGGTTTGTATTTTGTC
GGTGTTGAATATCCAGAATTTAGCTTCAAACGGCAAATAATTAAATTGTTTTGTTAG

Upstream 100 bases:

>100_bases
ATTTTGATAGTTTGGTTCATGGTTTTATCAATTTCTCAAAACTTATTCCAAAAGAAATGGATGTTCTGCATTCAAGAGTT
GTTAAATTTCTTGGCTAATT

Downstream 100 bases:

>100_bases
GTTTAATCTGATAATAATTATTCAATAGATATAAAATTATAAATAAAAGATAGCGATGAAAAAACTATCTACAATGGCAG
TAGTGTTTATTTCCACTGGG

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I [H]

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MKNYLLQIEYFGKNYCGWQRQSHSLSVQEELEKALSKIANQNIEVTCAGRTDTGVHATSQIVNFYSNAYRPLSAWQRGVN
ALLPQDIKILAVQQVDNNFNSRFTAINRTYNYIIYNSATSSPIFAEHCLWENRELDIDKMNQACEYLLGEQDFSSFRSSQ
CQSNTPFRNIQKAEFIKQGSFIVFEVVGNAFLHHMIRNLVGSLLKVGLGFESPEWIKVVLEAKDRTQAAETAKAHGLYFV
GVEYPEFSFKRQIIKLFC

Sequences:

>Translated_258_residues
MKNYLLQIEYFGKNYCGWQRQSHSLSVQEELEKALSKIANQNIEVTCAGRTDTGVHATSQIVNFYSNAYRPLSAWQRGVN
ALLPQDIKILAVQQVDNNFNSRFTAINRTYNYIIYNSATSSPIFAEHCLWENRELDIDKMNQACEYLLGEQDFSSFRSSQ
CQSNTPFRNIQKAEFIKQGSFIVFEVVGNAFLHHMIRNLVGSLLKVGLGFESPEWIKVVLEAKDRTQAAETAKAHGLYFV
GVEYPEFSFKRQIIKLFC
>Mature_258_residues
MKNYLLQIEYFGKNYCGWQRQSHSLSVQEELEKALSKIANQNIEVTCAGRTDTGVHATSQIVNFYSNAYRPLSAWQRGVN
ALLPQDIKILAVQQVDNNFNSRFTAINRTYNYIIYNSATSSPIFAEHCLWENRELDIDKMNQACEYLLGEQDFSSFRSSQ
CQSNTPFRNIQKAEFIKQGSFIVFEVVGNAFLHHMIRNLVGSLLKVGLGFESPEWIKVVLEAKDRTQAAETAKAHGLYFV
GVEYPEFSFKRQIIKLFC

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs [H]

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family [H]

Homologues:

Organism=Homo sapiens, GI23503259, Length=272, Percent_Identity=27.2058823529412, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI199559785, Length=282, Percent_Identity=25.531914893617, Blast_Score=79, Evalue=3e-15,
Organism=Escherichia coli, GI1788657, Length=241, Percent_Identity=50.207468879668, Blast_Score=254, Evalue=4e-69,
Organism=Saccharomyces cerevisiae, GI6321112, Length=276, Percent_Identity=27.536231884058, Blast_Score=86, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24648455, Length=235, Percent_Identity=27.2340425531915, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI281362146, Length=235, Percent_Identity=27.2340425531915, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI19922728, Length=265, Percent_Identity=25.6603773584906, Blast_Score=65, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094 [H]

Pfam domain/function: PF01416 PseudoU_synth_1 [H]

EC number: =5.4.99.12 [H]

Molecular weight: Translated: 29595; Mature: 29595

Theoretical pI: Translated: 7.83; Mature: 7.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNYLLQIEYFGKNYCGWQRQSHSLSVQEELEKALSKIANQNIEVTCAGRTDTGVHATSQ
CCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHH
IVNFYSNAYRPLSAWQRGVNALLPQDIKILAVQQVDNNFNSRFTAINRTYNYIIYNSATS
HHHHHHHCCCCHHHHHHHHHHCCCCCEEEEEEEHHCCCCCCCEEEEECEEEEEEECCCCC
SPIFAEHCLWENRELDIDKMNQACEYLLGEQDFSSFRSSQCQSNTPFRNIQKAEFIKQGS
CCHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCHHHHHHHHHHHCCC
FIVFEVVGNAFLHHMIRNLVGSLLKVGLGFESPEWIKVVLEAKDRTQAAETAKAHGLYFV
EEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHEEHHHHHHHHHHHHHHCCEEEE
GVEYPEFSFKRQIIKLFC
EECCCCHHHHHHHHHHCC
>Mature Secondary Structure
MKNYLLQIEYFGKNYCGWQRQSHSLSVQEELEKALSKIANQNIEVTCAGRTDTGVHATSQ
CCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHH
IVNFYSNAYRPLSAWQRGVNALLPQDIKILAVQQVDNNFNSRFTAINRTYNYIIYNSATS
HHHHHHHCCCCHHHHHHHHHHCCCCCEEEEEEEHHCCCCCCCEEEEECEEEEEEECCCCC
SPIFAEHCLWENRELDIDKMNQACEYLLGEQDFSSFRSSQCQSNTPFRNIQKAEFIKQGS
CCHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCHHHHHHHHHHHCCC
FIVFEVVGNAFLHHMIRNLVGSLLKVGLGFESPEWIKVVLEAKDRTQAAETAKAHGLYFV
EEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHEEHHHHHHHHHHHHHHCCEEEE
GVEYPEFSFKRQIIKLFC
EECCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA