Click here to switch to the map view.

The map label for this gene is pilD [H]

Identifier: 89256308

GI number: 89256308

Start: 926676

End: 927494

Strand: Reverse

Name: pilD [H]

Synonym: FTL_0959

Alternate gene names: 89256308

Gene position: 927494-926676 (Counterclockwise)

Preceding gene: 89256309

Following gene: 89256307

Centisome position: 48.92

GC content: 32.11

Gene sequence:

>819_bases
ATGTACTACGATATCTATATAATTTACTTGTTTATATTTCTATTTGGTGCTGCTATTGGCAGTTTTTTGAATGTTGTTAT
ATATCGAGTGCCAAATAAACTATTTGCTGATGAGCAAGCTATTGCACGAGAAATACTGGGGATTGATAAGCAACAATCTC
CACAGAATTTTAGTTTATTGACACCATCAAAATGCCCAAAATGTCATAATAAACTAAAGTATCGTCATAATATTCCGATA
ATCGGATGGTTTTTGCTTAAAGGCAAATGCTTTTTCTGTAAAGAAAAAATATCTTTTGAATACCCATTAATAGAGTTTAT
CACAGCTAGTTTATTTATAACTATTTTTTACTGTTTTGGTTTTACATTTCAGAGTTTAGCTCTAGTAACTTTGGCTAGCT
TTTTTATACCATTATTCTTTATTGATGCCAAACATCAAATCCTTCCTGATTCGTTAACACTACCATTACTATGGCTTGGG
ATAATACTTAATTACTACCATACGTTTACTACCCTAGAGCAATCTGTCTGGGGAGCTATAATTGGCTATCTTTCACTTTG
GTTGGTTTTTTGGATATACAAAATCCTTACAGGTAAAGAAGGCTTTGGTTATGGTGATTTTAAACTCTTAGCTGCAGTTG
GTGCTTGGTTTGGCTATCCAATGTTACTGTATACAATTTTCGCTAGTTGTATATTTGGGATTATAATCGCTATTGCTATA
AATCTTGTTGCTAAGCGTACTAATGTAATAGCTTTTGGGCCCGCGATAATTCTAGCAACATTTTTTTATCTACTGACTAA
AGATAATATCTATGTATAA

Upstream 100 bases:

>100_bases
CTACCCTACTATGGCTGAGGCTTATCGTATTGCTGGTATTGACGGTCTTAATAAGCTTAAACCTAAAAATAAACAATTTG
TCCCAGAATACCAATAATCC

Downstream 100 bases:

>100_bases
TATAATCACGTAATGCTAATTCAATCTTAATAATAACTAATGCAAAAAAAAAGCTTCTCTATTCTAGTTTTATTTGTTGT
TATACTATTAGTTGTACTGT

Product: Type IV pili leader peptidase and methylase.

Products: NA

Alternate protein names: Leader peptidase; Prepilin peptidase; N-methyltransferase [H]

Number of amino acids: Translated: 272; Mature: 272

Protein sequence:

>272_residues
MYYDIYIIYLFIFLFGAAIGSFLNVVIYRVPNKLFADEQAIAREILGIDKQQSPQNFSLLTPSKCPKCHNKLKYRHNIPI
IGWFLLKGKCFFCKEKISFEYPLIEFITASLFITIFYCFGFTFQSLALVTLASFFIPLFFIDAKHQILPDSLTLPLLWLG
IILNYYHTFTTLEQSVWGAIIGYLSLWLVFWIYKILTGKEGFGYGDFKLLAAVGAWFGYPMLLYTIFASCIFGIIIAIAI
NLVAKRTNVIAFGPAIILATFFYLLTKDNIYV

Sequences:

>Translated_272_residues
MYYDIYIIYLFIFLFGAAIGSFLNVVIYRVPNKLFADEQAIAREILGIDKQQSPQNFSLLTPSKCPKCHNKLKYRHNIPI
IGWFLLKGKCFFCKEKISFEYPLIEFITASLFITIFYCFGFTFQSLALVTLASFFIPLFFIDAKHQILPDSLTLPLLWLG
IILNYYHTFTTLEQSVWGAIIGYLSLWLVFWIYKILTGKEGFGYGDFKLLAAVGAWFGYPMLLYTIFASCIFGIIIAIAI
NLVAKRTNVIAFGPAIILATFFYLLTKDNIYV
>Mature_272_residues
MYYDIYIIYLFIFLFGAAIGSFLNVVIYRVPNKLFADEQAIAREILGIDKQQSPQNFSLLTPSKCPKCHNKLKYRHNIPI
IGWFLLKGKCFFCKEKISFEYPLIEFITASLFITIFYCFGFTFQSLALVTLASFFIPLFFIDAKHQILPDSLTLPLLWLG
IILNYYHTFTTLEQSVWGAIIGYLSLWLVFWIYKILTGKEGFGYGDFKLLAAVGAWFGYPMLLYTIFASCIFGIIIAIAI
NLVAKRTNVIAFGPAIILATFFYLLTKDNIYV

Specific function: Cleaves type-4 fimbrial leader sequence and methylates the N-terminal (generally Phe) residue [H]

COG id: COG1989

COG function: function code NOU; Type II secretory pathway, prepilin signal peptidase PulO and related peptidases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A24 family [H]

Homologues:

Organism=Escherichia coli, GI87082194, Length=266, Percent_Identity=33.8345864661654, Blast_Score=135, Evalue=4e-33,
Organism=Escherichia coli, GI1789732, Length=164, Percent_Identity=40.2439024390244, Blast_Score=86, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010627
- InterPro:   IPR014032
- InterPro:   IPR000045 [H]

Pfam domain/function: PF06750 DiS_P_DiS; PF01478 Peptidase_A24 [H]

EC number: =3.4.23.43 [H]

Molecular weight: Translated: 31236; Mature: 31236

Theoretical pI: Translated: 8.88; Mature: 8.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHCCEEEEHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure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HHHHHHCCEEEEHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8100347; 7906688; 7565116 [H]