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The map label for this gene is prs [H]

Identifier: 89256298

GI number: 89256298

Start: 918621

End: 919589

Strand: Direct

Name: prs [H]

Synonym: FTL_0949

Alternate gene names: 89256298

Gene position: 918621-919589 (Clockwise)

Preceding gene: 89256296

Following gene: 89256299

Centisome position: 48.45

GC content: 36.64

Gene sequence:

>969_bases
ATGTCAGAAGATTTGATGATTTTTAGCGGTAATGCTTCTAAAAAGCTTGCTAGTGAAGTAGCTAAAGAGCTAGGTGCAAC
TCTTGGTAATGCAACGGTTGATAGGTTTAAAGATGGCGAAATACATGTTGTCCTAAACGAGAATGTGCGTGGTAAGGATG
TATTTGTAATCCAATCAACTTGTCCACCATCTGATAATTTGATGGAACTTATTCTATTAATAGATGCGCTTAAAAGATCA
TCAGCAGAGAGGGTAACAGCAGTATTACCATATTTTGGCTATGCTAGGCAAGATAGAAGATCAAAATCAGCGAGAGTGCC
TATATCTGCTAAAGTTGTCGCAAATCTTCTTCAAGCTGTTGGCTTAGATAGGATATTATCAGTAGATATTCATGCTGAGC
AAATCCAAGGATTCTTTGACATACCATTTGATAATGCTTTTGCAACTAAAATATTCCTAGAATATGTGCGTAAAAATCCA
GAGAAATATCAAAATATCAAAATAGTATCACCTGACATGGGTGGTGTGGTTAGAGCTAGATCTGTAGCTAAAAACTTAGG
TGTTGAGATTGCTGTAGTTGATAAAAGAAGACCTAAACCAAATGTTGCAGAGGTTATGAACATAATTGGCGAAGTTGATG
GCAAACATTGTATACTTGTCGATGACATTATGGATACTGGTGGCACAATGTGTCAAGCAGCAAAAGCATTGATAGAGAAG
GGTGGAGCTGCTAAAGTATCAGCATTTTGTGTACATCCATTACTTTCTGGCGATGCGATTAAGAATATCGAGGATTCAGC
AATTGATGAACTCATAGTTACTGATTCTATACCTCTTAAACCTCATGCTGAAGCATGTAGCAAAATCAAAGTCATAACAT
TAGCACCATTACTTGCTCAAATTGTTGAAAAAACTAATGGAGAGGAATCAGTTAGTGATATTTTCCGTACTGATGGCTTA
GTTGATTAA

Upstream 100 bases:

>100_bases
ATAAAATAATTATCGAAATATTCTTTTTAGGCTTTTTTAAAATTAAAAAAAACATTAGAATTAGTATTTTGTAAGGTGTG
TGAATTTTTAAGGTTATAAG

Downstream 100 bases:

>100_bases
TTACTTGCATTAATAAATATAATCTACTACAATACAACAGTTAATCTAGGGTCGCATAGATTAAGTTTATTTGTTTAAAA
ATTAATAAGGAATCTAACAA

Product: ribose-phosphate pyrophosphokinase

Products: NA

Alternate protein names: RPPK; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase [H]

Number of amino acids: Translated: 322; Mature: 321

Protein sequence:

>322_residues
MSEDLMIFSGNASKKLASEVAKELGATLGNATVDRFKDGEIHVVLNENVRGKDVFVIQSTCPPSDNLMELILLIDALKRS
SAERVTAVLPYFGYARQDRRSKSARVPISAKVVANLLQAVGLDRILSVDIHAEQIQGFFDIPFDNAFATKIFLEYVRKNP
EKYQNIKIVSPDMGGVVRARSVAKNLGVEIAVVDKRRPKPNVAEVMNIIGEVDGKHCILVDDIMDTGGTMCQAAKALIEK
GGAAKVSAFCVHPLLSGDAIKNIEDSAIDELIVTDSIPLKPHAEACSKIKVITLAPLLAQIVEKTNGEESVSDIFRTDGL
VD

Sequences:

>Translated_322_residues
MSEDLMIFSGNASKKLASEVAKELGATLGNATVDRFKDGEIHVVLNENVRGKDVFVIQSTCPPSDNLMELILLIDALKRS
SAERVTAVLPYFGYARQDRRSKSARVPISAKVVANLLQAVGLDRILSVDIHAEQIQGFFDIPFDNAFATKIFLEYVRKNP
EKYQNIKIVSPDMGGVVRARSVAKNLGVEIAVVDKRRPKPNVAEVMNIIGEVDGKHCILVDDIMDTGGTMCQAAKALIEK
GGAAKVSAFCVHPLLSGDAIKNIEDSAIDELIVTDSIPLKPHAEACSKIKVITLAPLLAQIVEKTNGEESVSDIFRTDGL
VD
>Mature_321_residues
SEDLMIFSGNASKKLASEVAKELGATLGNATVDRFKDGEIHVVLNENVRGKDVFVIQSTCPPSDNLMELILLIDALKRSS
AERVTAVLPYFGYARQDRRSKSARVPISAKVVANLLQAVGLDRILSVDIHAEQIQGFFDIPFDNAFATKIFLEYVRKNPE
KYQNIKIVSPDMGGVVRARSVAKNLGVEIAVVDKRRPKPNVAEVMNIIGEVDGKHCILVDDIMDTGGTMCQAAKALIEKG
GAAKVSAFCVHPLLSGDAIKNIEDSAIDELIVTDSIPLKPHAEACSKIKVITLAPLLAQIVEKTNGEESVSDIFRTDGLV
D

Specific function: Utilized by both the de novo and the salvage pathways by which endogenously formed or exogenously added pyrimidine, purine, or pyridine bases are converted to the corresponding ribonucleoside monophosphates. [C]

COG id: COG0462

COG function: function code FE; Phosphoribosylpyrophosphate synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribose-phosphate pyrophosphokinase family [H]

Homologues:

Organism=Homo sapiens, GI4506129, Length=313, Percent_Identity=45.3674121405751, Blast_Score=279, Evalue=2e-75,
Organism=Homo sapiens, GI84875539, Length=315, Percent_Identity=44.7619047619048, Blast_Score=278, Evalue=7e-75,
Organism=Homo sapiens, GI4506127, Length=313, Percent_Identity=44.7284345047923, Blast_Score=277, Evalue=1e-74,
Organism=Homo sapiens, GI28557709, Length=313, Percent_Identity=43.7699680511182, Blast_Score=267, Evalue=8e-72,
Organism=Homo sapiens, GI4506133, Length=353, Percent_Identity=33.9943342776204, Blast_Score=181, Evalue=6e-46,
Organism=Homo sapiens, GI194018537, Length=345, Percent_Identity=32.7536231884058, Blast_Score=170, Evalue=1e-42,
Organism=Homo sapiens, GI310128524, Length=140, Percent_Identity=32.8571428571429, Blast_Score=79, Evalue=7e-15,
Organism=Homo sapiens, GI310115209, Length=140, Percent_Identity=32.8571428571429, Blast_Score=79, Evalue=7e-15,
Organism=Homo sapiens, GI310118259, Length=140, Percent_Identity=32.8571428571429, Blast_Score=79, Evalue=7e-15,
Organism=Homo sapiens, GI310119946, Length=140, Percent_Identity=32.8571428571429, Blast_Score=79, Evalue=7e-15,
Organism=Escherichia coli, GI1787458, Length=314, Percent_Identity=54.140127388535, Blast_Score=362, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI25149168, Length=313, Percent_Identity=43.7699680511182, Blast_Score=270, Evalue=8e-73,
Organism=Caenorhabditis elegans, GI17554702, Length=313, Percent_Identity=43.7699680511182, Blast_Score=270, Evalue=9e-73,
Organism=Caenorhabditis elegans, GI17554704, Length=310, Percent_Identity=44.1935483870968, Blast_Score=269, Evalue=1e-72,
Organism=Caenorhabditis elegans, GI71989924, Length=313, Percent_Identity=43.7699680511182, Blast_Score=269, Evalue=2e-72,
Organism=Caenorhabditis elegans, GI17570245, Length=343, Percent_Identity=31.7784256559767, Blast_Score=176, Evalue=1e-44,
Organism=Saccharomyces cerevisiae, GI6321776, Length=317, Percent_Identity=37.8548895899054, Blast_Score=232, Evalue=6e-62,
Organism=Saccharomyces cerevisiae, GI6319403, Length=312, Percent_Identity=37.8205128205128, Blast_Score=223, Evalue=3e-59,
Organism=Saccharomyces cerevisiae, GI6320946, Length=317, Percent_Identity=36.2776025236593, Blast_Score=218, Evalue=9e-58,
Organism=Saccharomyces cerevisiae, GI6322667, Length=196, Percent_Identity=38.265306122449, Blast_Score=143, Evalue=4e-35,
Organism=Saccharomyces cerevisiae, GI6324511, Length=115, Percent_Identity=36.5217391304348, Blast_Score=81, Evalue=2e-16,
Organism=Drosophila melanogaster, GI21355239, Length=313, Percent_Identity=44.408945686901, Blast_Score=263, Evalue=1e-70,
Organism=Drosophila melanogaster, GI45551540, Length=335, Percent_Identity=41.4925373134328, Blast_Score=253, Evalue=1e-67,
Organism=Drosophila melanogaster, GI281362873, Length=365, Percent_Identity=32.3287671232877, Blast_Score=185, Evalue=5e-47,
Organism=Drosophila melanogaster, GI24651454, Length=365, Percent_Identity=32.3287671232877, Blast_Score=185, Evalue=5e-47,
Organism=Drosophila melanogaster, GI24651458, Length=365, Percent_Identity=32.3287671232877, Blast_Score=184, Evalue=5e-47,
Organism=Drosophila melanogaster, GI24651456, Length=365, Percent_Identity=32.3287671232877, Blast_Score=184, Evalue=5e-47,
Organism=Drosophila melanogaster, GI45552010, Length=384, Percent_Identity=30.46875, Blast_Score=176, Evalue=2e-44,
Organism=Drosophila melanogaster, GI24651462, Length=384, Percent_Identity=30.46875, Blast_Score=176, Evalue=2e-44,
Organism=Drosophila melanogaster, GI24651464, Length=384, Percent_Identity=30.46875, Blast_Score=176, Evalue=2e-44,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000842
- InterPro:   IPR005946
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00156 Pribosyltran [H]

EC number: =2.7.6.1 [H]

Molecular weight: Translated: 34910; Mature: 34779

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00114 PRPP_SYNTHETASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEDLMIFSGNASKKLASEVAKELGATLGNATVDRFKDGEIHVVLNENVRGKDVFVIQST
CCCCEEEEECCCHHHHHHHHHHHHHHHHCCCHHHHCCCCCEEEEECCCCCCCEEEEEECC
CPPSDNLMELILLIDALKRSSAERVTAVLPYFGYARQDRRSKSARVPISAKVVANLLQAV
CCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
GLDRILSVDIHAEQIQGFFDIPFDNAFATKIFLEYVRKNPEKYQNIKIVSPDMGGVVRAR
CHHHEEEEEEEHHHCCCEEECCCCCHHHHHHHHHHHHCCHHHHCCEEEECCCCCCHHHHH
SVAKNLGVEIAVVDKRRPKPNVAEVMNIIGEVDGKHCILVDDIMDTGGTMCQAAKALIEK
HHHHHCCEEEEEECCCCCCCCHHHHHHHHHCCCCCEEEEEHHHHCCCCHHHHHHHHHHHC
GGAAKVSAFCVHPLLSGDAIKNIEDSAIDELIVTDSIPLKPHAEACSKIKVITLAPLLAQ
CCCCEEHHHHHHHHHCCHHHCCCCHHHHHHEEECCCCCCCCCHHHHHHEEHHHHHHHHHH
IVEKTNGEESVSDIFRTDGLVD
HHHHCCCHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SEDLMIFSGNASKKLASEVAKELGATLGNATVDRFKDGEIHVVLNENVRGKDVFVIQST
CCCEEEEECCCHHHHHHHHHHHHHHHHCCCHHHHCCCCCEEEEECCCCCCCEEEEEECC
CPPSDNLMELILLIDALKRSSAERVTAVLPYFGYARQDRRSKSARVPISAKVVANLLQAV
CCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
GLDRILSVDIHAEQIQGFFDIPFDNAFATKIFLEYVRKNPEKYQNIKIVSPDMGGVVRAR
CHHHEEEEEEEHHHCCCEEECCCCCHHHHHHHHHHHHCCHHHHCCEEEECCCCCCHHHHH
SVAKNLGVEIAVVDKRRPKPNVAEVMNIIGEVDGKHCILVDDIMDTGGTMCQAAKALIEK
HHHHHCCEEEEEECCCCCCCCHHHHHHHHHCCCCCEEEEEHHHHCCCCHHHHHHHHHHHC
GGAAKVSAFCVHPLLSGDAIKNIEDSAIDELIVTDSIPLKPHAEACSKIKVITLAPLLAQ
CCCCEEHHHHHHHHHCCHHHCCCCHHHHHHEEECCCCCCCCCHHHHHHEEHHHHHHHHHH
IVEKTNGEESVSDIFRTDGLVD
HHHHCCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]