Click here to switch to the map view.

The map label for this gene is tilS [H]

Identifier: 89256102

GI number: 89256102

Start: 702998

End: 704194

Strand: Reverse

Name: tilS [H]

Synonym: FTL_0713

Alternate gene names: 89256102

Gene position: 704194-702998 (Counterclockwise)

Preceding gene: 89256103

Following gene: 89256099

Centisome position: 37.14

GC content: 30.24

Gene sequence:

>1197_bases
ATGTCTATAAGCAAATCTCTTGTTTTAAATGAAATAAAAAAATTCTCGCCTTCTCATATTATTATTGGTTATAGTGGTGG
CGTTGACTCAAGTGTTTTACTAAATATTAGCAAAGAATTAGATATTCCACTGATTGCAATTTATATTAATCATAATCTTC
ATCGTGACTCATTAAAATGGCAAATTCATTGTCAACAAACTTGTCAAAAATATAATCTACAATTTATTAGCCACTCATTA
GATAAAGTTCCTAAGGGTGAGAGTTTTGAGGCTTGGGCAAGTAAACAAAGAATGGCATTTTTTCAAAAAATAATGCAGCA
GTACTCTAAGCCACTACTTCTATTAGGACATCACCAAGATGATCAAGCCGAGACATTCTTAATTCAAGCTATACGTGGCT
CGGGATTAGCAGGCTTAGCAAGTATCCCACATTATAAGGAACTTCATCATGGAGGTGTATTACGACCATTATTAAAATAT
AGTAAAATTGATATTGAAGGGTTTGCTAAACTAAATAATATTTCATATATATATGATGATAGTAATGAAGATATAAAATA
CCGCAGAAACTTAATTCGCAATCAAATAATCCCGATACTACAGCAAGTAAATCCAAATATCAGTCAAACCCTCTCTCGTA
GTGCAAATATTTGTGCAGAAAGTAATAATATTTTGCAAAAGCTCCTTACAGAAAGATTACAATCGATATCTCAAGATACT
AATCTAATAATTAGCGAGTTAATAAAATTAGATGATGATATTCAAAAAAATCTTCTGCATCTTTGGTTTAAACAAAATAC
TCAACAAAGTCTTAAAAGTAAGCAAATAAAAGAGTTACATCTAGCTGTCAATAACCCCTCAACAGGTTGGCAAATCGATA
TAAGTAACTATTATCAAATTCACATCCAATATAATCAGCTTATAATAAAGTATCCAACAACTATCAATGATATAAGCAAA
GAAGATATCATAAGTTGGCTTAGCAAAAATCTTAATGAAGAAATCGATCTAACCAAAATAGTAATTCGTGATAGAAAGCC
TGATGATAAATGCAAATATCGTGGCAGAAATAAGCCTAATAAACTCAAAATACTCTTTCAAGAACTACAAATTCCTACCA
CTGAAAGATCAAAAGCAAAAATAATTTTAAAAGATCAGCAAATAATCGCTGTATATCCTTTTTTTATTTGTGGGTAA

Upstream 100 bases:

>100_bases
TGTTGTTATGGATATTAAATCAAGCTCAGATGAGGCTAAAGAACTTATTGATGAATTCAAAAAAGTTAAAGCTACCATAA
AAGCCCGTTATTTAGTATAA

Downstream 100 bases:

>100_bases
TTTTTAACTACTTTAATTTCTTTATGATTAAAGCAACAATATACCATAGTATAATTGTCACTGCCAAGATAGCCATGATA
TTGGTAAATAGCCCTTGGAA

Product: PP-loop family protein

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 398; Mature: 397

Protein sequence:

>398_residues
MSISKSLVLNEIKKFSPSHIIIGYSGGVDSSVLLNISKELDIPLIAIYINHNLHRDSLKWQIHCQQTCQKYNLQFISHSL
DKVPKGESFEAWASKQRMAFFQKIMQQYSKPLLLLGHHQDDQAETFLIQAIRGSGLAGLASIPHYKELHHGGVLRPLLKY
SKIDIEGFAKLNNISYIYDDSNEDIKYRRNLIRNQIIPILQQVNPNISQTLSRSANICAESNNILQKLLTERLQSISQDT
NLIISELIKLDDDIQKNLLHLWFKQNTQQSLKSKQIKELHLAVNNPSTGWQIDISNYYQIHIQYNQLIIKYPTTINDISK
EDIISWLSKNLNEEIDLTKIVIRDRKPDDKCKYRGRNKPNKLKILFQELQIPTTERSKAKIILKDQQIIAVYPFFICG

Sequences:

>Translated_398_residues
MSISKSLVLNEIKKFSPSHIIIGYSGGVDSSVLLNISKELDIPLIAIYINHNLHRDSLKWQIHCQQTCQKYNLQFISHSL
DKVPKGESFEAWASKQRMAFFQKIMQQYSKPLLLLGHHQDDQAETFLIQAIRGSGLAGLASIPHYKELHHGGVLRPLLKY
SKIDIEGFAKLNNISYIYDDSNEDIKYRRNLIRNQIIPILQQVNPNISQTLSRSANICAESNNILQKLLTERLQSISQDT
NLIISELIKLDDDIQKNLLHLWFKQNTQQSLKSKQIKELHLAVNNPSTGWQIDISNYYQIHIQYNQLIIKYPTTINDISK
EDIISWLSKNLNEEIDLTKIVIRDRKPDDKCKYRGRNKPNKLKILFQELQIPTTERSKAKIILKDQQIIAVYPFFICG
>Mature_397_residues
SISKSLVLNEIKKFSPSHIIIGYSGGVDSSVLLNISKELDIPLIAIYINHNLHRDSLKWQIHCQQTCQKYNLQFISHSLD
KVPKGESFEAWASKQRMAFFQKIMQQYSKPLLLLGHHQDDQAETFLIQAIRGSGLAGLASIPHYKELHHGGVLRPLLKYS
KIDIEGFAKLNNISYIYDDSNEDIKYRRNLIRNQIIPILQQVNPNISQTLSRSANICAESNNILQKLLTERLQSISQDTN
LIISELIKLDDDIQKNLLHLWFKQNTQQSLKSKQIKELHLAVNNPSTGWQIDISNYYQIHIQYNQLIIKYPTTINDISKE
DIISWLSKNLNEEIDLTKIVIRDRKPDDKCKYRGRNKPNKLKILFQELQIPTTERSKAKIILKDQQIIAVYPFFICG

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=420, Percent_Identity=27.1428571428571, Blast_Score=150, Evalue=2e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR015262
- InterPro:   IPR020825
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3; PF09179 DUF1946; PF11734 TilS_C [H]

EC number: NA

Molecular weight: Translated: 46040; Mature: 45909

Theoretical pI: Translated: 9.58; Mature: 9.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSISKSLVLNEIKKFSPSHIIIGYSGGVDSSVLLNISKELDIPLIAIYINHNLHRDSLKW
CCCCHHHHHHHHHHCCCCEEEEEECCCCCCEEEEEECCCCCCCEEEEEEECCCCCCCCEE
QIHCQQTCQKYNLQFISHSLDKVPKGESFEAWASKQRMAFFQKIMQQYSKPLLLLGHHQD
EEEHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCC
DQAETFLIQAIRGSGLAGLASIPHYKELHHGGVLRPLLKYSKIDIEGFAKLNNISYIYDD
CHHHHHHHHHHHCCCCCCHHCCCCHHHHHCCCHHHHHHHHHCCCHHHHHEECCEEEEEEC
SNEDIKYRRNLIRNQIIPILQQVNPNISQTLSRSANICAESNNILQKLLTERLQSISQDT
CCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHCCCHHHHHHHHHHHHHHHCCCH
NLIISELIKLDDDIQKNLLHLWFKQNTQQSLKSKQIKELHLAVNNPSTGWQIDISNYYQI
HHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHEEEEEECCCCCCEEEEECCEEEE
HIQYNQLIIKYPTTINDISKEDIISWLSKNLNEEIDLTKIVIRDRKPDDKCKYRGRNKPN
EEEEEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHCCCCCCCH
KLKILFQELQIPTTERSKAKIILKDQQIIAVYPFFICG
HHHHHHHHHCCCCCCCCCEEEEEECCEEEEEECEEECC
>Mature Secondary Structure 
SISKSLVLNEIKKFSPSHIIIGYSGGVDSSVLLNISKELDIPLIAIYINHNLHRDSLKW
CCCHHHHHHHHHHCCCCEEEEEECCCCCCEEEEEECCCCCCCEEEEEEECCCCCCCCEE
QIHCQQTCQKYNLQFISHSLDKVPKGESFEAWASKQRMAFFQKIMQQYSKPLLLLGHHQD
EEEHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCC
DQAETFLIQAIRGSGLAGLASIPHYKELHHGGVLRPLLKYSKIDIEGFAKLNNISYIYDD
CHHHHHHHHHHHCCCCCCHHCCCCHHHHHCCCHHHHHHHHHCCCHHHHHEECCEEEEEEC
SNEDIKYRRNLIRNQIIPILQQVNPNISQTLSRSANICAESNNILQKLLTERLQSISQDT
CCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHCCCHHHHHHHHHHHHHHHCCCH
NLIISELIKLDDDIQKNLLHLWFKQNTQQSLKSKQIKELHLAVNNPSTGWQIDISNYYQI
HHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHEEEEEECCCCCCEEEEECCEEEE
HIQYNQLIIKYPTTINDISKEDIISWLSKNLNEEIDLTKIVIRDRKPDDKCKYRGRNKPN
EEEEEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHCCCCCCCH
KLKILFQELQIPTTERSKAKIILKDQQIIAVYPFFICG
HHHHHHHHHCCCCCCCCCEEEEEECCEEEEEECEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA